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Citations for BioLegend :
151 - 200 of 4140 citations for GM CSF Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... and 20ng/mL recombinant murine M-CSF (Shenandoah 200-08 or BioLegend 576404) in non-TC treated Petri dishes ...
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bioRxiv - Microbiology 2021Quote: ... Binding of His-tagged S1-RBD was detected with Alexa Fluor 488 anti-his tag antibody (clone J095G46, Biolegend, UK) 1:5000 in 25 μL PBST + 0.1% BSA per well ...
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bioRxiv - Immunology 2022Quote: ... This is followed by secondary staining for 30 min at 4°C with APC-conjugated mouse anti-His antibody (BioLegend, #362605, 1:50) or isotype control ...
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bioRxiv - Immunology 2020Quote: ... APC-anti-his tag antibody (Biolegend, clone: J095G46)) ...
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bioRxiv - Biochemistry 2022Quote: ... and Purified anti-His Tag antibody (BioLegend 652502) for 1 hour in blocking buffer shaking at rt ...
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bioRxiv - Microbiology 2023Quote: ... Phycoerythrin (PE) anti-His tag antibody (BioLegend 362603) was added to the cell suspension at a dilution of 1:20 and incubated for 30 minutes on ice in the dark ...
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bioRxiv - Immunology 2020Quote: ... BMDMs were generated by culturing BM cells with rmM-CSF (20 ng/ml, BioLegend) and BMDCs were generated with rmGM-CSF (20 ng/ml ...
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bioRxiv - Immunology 2022Quote: ... 50 μg/mL streptomycin and supplemented with 10ng/ml M-CSF (Biolegend; cat#576402) at time of culture and again at day 3 of culture ...
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bioRxiv - Pathology 2023Quote: ... 50 ng/ml macrophage colony-stimulating factor (M-CSF, BioLegend, San Diego, CA, USA), 50 U/ml penicillin/streptomycin for 7 days.
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bioRxiv - Microbiology 2021Quote: ... 100μl of HRP anti-His Tag Antibody (BioLegend, USA) (diluted 1:20000 ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... and a HRP anti-His tag antibody (Biolegend, #652504) diluted 1:1000 in Superblock (Thermo #37515 ...
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bioRxiv - Bioengineering 2020Quote: ... HRP conjugated anti-His tag secondary antibody (BioLegend, 652503) diluted 1:2000 in blocking buffer was then added to the plates and incubated at RT for 1 hour ...
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bioRxiv - Bioengineering 2024Quote: ... we used HRP anti-His tag antibody (BioLegend 652503).
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bioRxiv - Cancer Biology 2022Quote: ... 50 μL of macrophage colony stimulating factor (M-CSF) (100 ng/mL, BioLegend, cat. 574802) was added and replenished every other day for four days by substituting the half of the medium with fresh medium containing M-CSF ...
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bioRxiv - Immunology 2022Quote: ... life technologies) or Legendplex (M1 macrophage panel, with added G-CSF capture beads,740848, Biolegend) in accordance with the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... and transduction with the M-CSF construct was measured with anti-Thy1.1 PE (B336285, BioLegend). M-CSF differentiated macrophages were stained with Fc block and Oligoblock (made in-house) ...
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bioRxiv - Immunology 2024Quote: ... B-mercaptoethanol and Pen/Strep in the presence of 100ng/ml M-CSF (576406, Biolegend) followed by 3 more days with renewed media and cytokines ...
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bioRxiv - Bioengineering 2023Quote: ... incubating the cells with APC anti-His Tag Antibody (BioLegend) for 20 minutes on ice ...
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bioRxiv - Immunology 2020Quote: ... 100 U/mL penicillin and 100 μg/mL streptomycin supplemented with 20 ng/mL M-CSF (BioLegend) at a density of 2×105 cells/mL ...
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bioRxiv - Immunology 2022Quote: ... and M2 macrophages with 20ng/mL of macrophage –colony-stimulating factor (M-CSF, Cat. No. 574804, BioLegend). After 6 days ...
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bioRxiv - Cell Biology 2019Quote: HEK293 cells stably expressing EPO-eGFP and A1At-mCherry were incubated with 1ug/mL Brefeldin A (Biolegend) for 12 hr ...
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bioRxiv - Cell Biology 2022Quote: ... Bone marrow cells were differentiated into BMDMs using 40 ng/mL recombinant M-CSF (BioLegend, catalog number 576406) in the same media formulation as TR-AMs ...
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bioRxiv - Immunology 2024Quote: ... and 10 ng/ml recombinant macrophage colony-stimulating factor (M-CSF; Cat# 576402; BioLegend, San Diego, CA, USA) for 7 days ...
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bioRxiv - Microbiology 2020Quote: ... The cells were differentiated into macrophages by incubation with 50 ng/ml macrophage colony–stimulating factor (M-CSF) (BioLegend) in Dulbecco’s Modified Eagle’s Medium (DMEM ...
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bioRxiv - Immunology 2022Quote: ... Monocytes from BM were differentiated into macrophages with the addition of 20 μg/ml M-CSF (Cat#556904, BioLegend) after 7 days of culturing ...
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bioRxiv - Immunology 2022Quote: ... was counted in an automated Vicell cell counter before 2X106 cells/well were cultured in Iscove’s Modified Dulbecco’s Medium (IMDM) supplemented with 10% FBS and 10ng/ml Macrophage colony- stimulating factor (M-CSF) (Biolegend), (growth media ...
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bioRxiv - Biochemistry 2024Quote: ... the BMMs were seeded into 6-well plates and incubated with macrophage colony-stimulating factor (M-CSF) (20_ng/ml, Biolegend) and Receptor activator of nuclear factor kappa-Β ligand (RANKL ...
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bioRxiv - Cell Biology 2020Quote: ... before plating the cells in petri dishes in RPMI 1640 medium containing 10 % heat-inactivated FCS and 50 ng/ml M-CSF (BioLegend) or 50 ng/ml GM-CSF (BioLegend) ...
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bioRxiv - Immunology 2021Quote: ... were produced by differentiating peripheral blood mononuclear cells (PBMC) in RPMI 1640 medium containing human M-CSF (10ng/mL, Biolegend) and 10% human platelets-free plasma for 5-7 days (42 ...
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bioRxiv - Immunology 2019Quote: ... Some cultures were supplemented with combinations of 8µg/ml IL-34 (Generon, UK), 100 µg/ml BMP7 (RD systems, USA and 10µg/ml CSF-1 (Biolegend, USA) for the duration of the culture.
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bioRxiv - Immunology 2020Quote: ... IFN-γ or GM-CSF release in the cell culture supernatant was measured using ELISA according to the manufacturer’s protocol (Sanquin and BioLegend, respectively).
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bioRxiv - Bioengineering 2022Quote: ... Cytokine-primed BMDMs were cultured in RPMI growth media + 20 ng/mL M-CSF + 20 ng/mL IFNγ (BioLegend 575302) or 20 ng/mL IL-4 (BioLegend 574302 ...
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bioRxiv - Physiology 2023Quote: ... zoledronic acid (Novartis Pharmaceuticals) and/or 50 μg/kg (i.p.) recombinant human granulocyte-colony stimulating factor (G-CSF; carrier-free, BioLegend #578604) for three days ...
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bioRxiv - Immunology 2023Quote: ... Live/dead staining and the M-CSF CAR-M viability assay was conducted with ZombieNIR Fixable Viabilty Dye (423106, Biolegend). Flow cytometry data was acquired on a Cytek NL-3000 ...
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bioRxiv - Synthetic Biology 2021Quote: ... Anti-bodies used in this study included a monoclonal α-his-antibody (BioLegend, 652502) and polyclonal α-CsgA-antibody and α-TasA-antibody.
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bioRxiv - Bioengineering 2022Quote: ... Confirmation of successful bead conjugation was determined by staining with anti-His Tag (Biolegend) and flow cytometry analysis ...
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bioRxiv - Pharmacology and Toxicology 2019Quote: ... The Western blot membranes were stained with HRP anti-His tag antibody (Biolegend, #652504) diluted 1:2,000 in 0.5% (w/v ...
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bioRxiv - Cell Biology 2021Quote: ... non-adherent cells were moved to bacteriological Petri dishes the next day and differentiated over 1 week in cDMEM containing 20 ng/mL recombinant murine M-CSF (Biolegend, 576404). Generation of murine bone-marrow derived dendritic cells (BMDC ...
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bioRxiv - Cancer Biology 2022Quote: ... 0.01% 55 mM 2-mercaptoethanol) supplemented with rmGM-CSF (50 ng/mL) and rmFlt3-L (200ng/mL) (Biolegend, San Diego CA). The culture medium was changed on day 9 of culture ...
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bioRxiv - Immunology 2023Quote: ... MΦs were generated by incubating bone marrow cells at 37°C with 40 ng/ml M-CSF (Biolegend, San Diego, CA) in complete RPMI 1640 medium (Gibco ...
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bioRxiv - Cell Biology 2024Quote: ... and the resulting suspension was plated at 1-2×106 cells/ml in complete medium containing 20% FCS and 20 ng/ml M-CSF (BioLegend, 574804). Cells were incubated for 6 days with a medium replacement at day 3 ...
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bioRxiv - Bioengineering 2024Quote: ... followed by the staining with anti-His-APC (1:20, clone J095G46, BioLegend cat# 362605) with Fc block (1:20 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Mononuclear cells were collected and washed with complete medium and 104 cells per well in 96 wells plate were plated in the presence of 40ng/ml M-CSF(Biolegend; cat#576402) and 60ng/ml RANKL (R&D Systems ...
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bioRxiv - Immunology 2024Quote: ... were generated from the femur’s bone marrow single cell suspension,n cultured in the complete RPMI with 20 ng/ml of rmGM-CSF (Biolegend, cat#576308) for 9 days.
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bioRxiv - Immunology 2021Quote: ... RBD was then detected using an Alexa Fluor 488 conjugated anti-His antibody (clone J099B1, Biolegend). Cells were analyzed on a flow cytometry (BD).
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bioRxiv - Biochemistry 2022Quote: ... one slide was probed with Alexa Fluor® 647 anti-his antibody (1:250, BioLegend, 362611). Sera/plasma samples were single or serial diluted (three-fold dilutions from 1:90 ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were passed through 70-micron cell strainer and subsequently cultured 10mL complete RPMI media supplemented with 20 ng/mL of recombinant murine M-CSF (576406; BioLegend, 315-02 PeproTech). On day 3 ...
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bioRxiv - Neuroscience 2023Quote: ... blood cells were lysed using VersaLyse buffer and blood and CSF cells were stained using the following anti-human antibodies (Biolegend; clone names indicated): CD3 (UCHT1) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and a further 1 h incubation with anti-His HRP conjugate (652504; BioLegend, San Diego, CA, USA) diluted (1:20000 ...
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bioRxiv - Bioengineering 2023Quote: The antibodies used for flow cytometry were as follows: anti-His Tag APC (BioLegend, USA, Cat: #362605), anti-human IgG (G18-145 ...