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Citations for BioLegend :
51 - 100 of 4140 citations for GM CSF Mouse HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... granulocyte macrophage colony-stimulating factor (GM-CSF, catalogue no. 578602; BioLegend), 1 ng/ml interleukin-6 (IL6 ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were cultured with 20 ng/mL GM-CSF (Biolegend, 576302) for 7 days with DMEM (5% pen/strep ...
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bioRxiv - Cancer Biology 2024Quote: ... Media were supplemented with 2 ng/ml GM-CSF (BioLegend 572902) for OCI-AML5 ...
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bioRxiv - Immunology 2022Quote: Bone marrow cells from femurs and tibias were cultured in presence of 20 ng/ml mouse recombinant GM-CSF (Biolegend) for 8 days ...
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bioRxiv - Immunology 2020Quote: ... and were cultured with 50 ng/ml recombinant human GM-CSF (BioLegend #572904) and 50 ng/ml recombinant human M-CSF (BioLegend #574806 ...
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bioRxiv - Immunology 2019Quote: ... this medium was supplemented with GM-CSF (40 ng/mL, Biolegend Catalog # 575906), and IL-13 (80 ng/mL ...
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bioRxiv - Immunology 2023Quote: ... Culture medium is supplemented with 1000U/well murine GM-CSF (Biolegend, Cat#576302) and a predetermined dose of P815-IL4 supernatant ...
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bioRxiv - Cancer Biology 2023Quote: ... and 50 ng/mL granulocyte macrophage colony stimulating factor (GM–CSF, for M1 polarization) or 50 ng/mL macrophage colony-stimulating factor (M–CSF, for M2 polarization) (BioLegend, San Diego ...
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bioRxiv - Cancer Biology 2019Quote: ... The cells were washed with HBSS twice by centrifugation at 250 x g for 8 min and then suspended in BMDC culture medium (RPMI-1640 medium [Thermo Fisher Scientific] containing 10% FBS, 20 ng/ml recombinant mouse GM-CSF [BioLegend] ...
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bioRxiv - Microbiology 2020Quote: ... and 50 ng/ml GM-CSF + 500 U/ml IL-4 (Biolegend, https://www.biolegend.com), respectively ...
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bioRxiv - Cell Biology 2021Quote: ... 20 ng/ml granulocyte-macrophage colony-stimulating factor (GM-CSF) (Biolegend, San Diego, CA) and 10 ng/ml IL-4 (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... or mouse G-CSF (BioLegend). The blocking of H3 and G-CSF was performed by preincubating the different 3% plasma with 0.5 µg/ml of anti-hH3 ...
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bioRxiv - Genomics 2024Quote: ... expanded CD34+ cells were cultured in basal medium with 20 ng/μl GM-CSF (Biolegend) and 1 x CC100 (StemCell Technologies ...
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bioRxiv - Immunology 2022Quote: ... and resulting cells were cultured in complete RPMI-1640 medium containing various concentrations of recombinant murine GM-CSF (rGM-CSF, BioLegend, San Diego, CA, USA), GPI-GM-CSF ...
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bioRxiv - Immunology 2021Quote: ... For intracellular cytokine staining the following antibodies were used: anti-GM-CSF-PE (MP1-22E9, BioLegend); anti-IL-17A (TC11-18H10.1 ...
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bioRxiv - Immunology 2023Quote: ... supplemented with 10% FBS in presence of GM-CSF (20 ng ml−1, BioLegend Cat# 572903) and IL-4 (20 ng ml−1 ...
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The loss-of-function of SOCS2 increases the inflammatory response to Staphylococcus aureus infectionbioRxiv - Immunology 2023Quote: ... Individual cytokine detection kits were also used to quantify mouse IL-6 and TNF-α (Bio-techne, France) and GM-CSF (Biolegend, San Diego, USA).
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bioRxiv - Immunology 2022Quote: ... 96-well ELISA plates were coated with 3 μg/ml GPI-RBD-GM-CSF in coating buffer (BioLegend) overnight at 4 °C ...
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bioRxiv - Pathology 2021Quote: ... followed by detection of GM-CSF with HRP-conjugated detection antibody and TMB solution according to the manufacturer’s instructions (Biolegend).
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bioRxiv - Bioengineering 2022Quote: ... BV785), GM-CSF (MP1-22E9, PE-Cy7), CD49b (DX5, PE) and CD94 (18d3, PerCP/Cy5.5) were purchased from BioLegend. Anti-Ly49I (YLI-90 ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: Both FLT3L and GM-CSF BMDCs were stained with 1:1000 Zombie Green Fixable Viability Kit (BioLegend, Cat# 423111) and were blocked with anti-mouse CD16/32 (BioLegend ...
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bioRxiv - Microbiology 2020Quote: ... cells were counted and plated on 6 well plates in RPMI + 5% FBS + 20 ng/ml GM-CSF (BioLegend). Cells were maintained at 37° C 5% CO2 and supplemented on day 3 ...
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bioRxiv - Immunology 2019Quote: ... 2 ml medium/well) were cultured in growth media supplemented with GM-CSF (100 ng/ml, BioLegend, San Diego), TGF-β (10 ng/ml ...
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bioRxiv - Genetics 2021Quote: ... and 20 ng/ml mouse M-CSF (BioLegend) for the purpose of differentiation ...
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bioRxiv - Immunology 2019Quote: ... The presence of human GM-CSF in the supernatant of eMSCs co-cultures with CD34+ HSPCs was assessed by enzyme-linked immunosorbent assay (ELISA) using the human GM-CSF ELISA MAX kit (Biolegend) as per manufacturer’s instructions.
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bioRxiv - Immunology 2020Quote: ... Cytokine content of cell free supernatants was determined using standard sandwich ELISA according to the manufacturer’s instructions (IFN-γ and GM-CSF; Sanquin and Biolegend). Some target cells were pre-incubated for 2 days in the presence or absence of UGCG inhibitors.
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bioRxiv - Immunology 2022Quote: ... were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS) in the presence of 30 ng/ml GM-CSF (BioLegend) for 9-12 days ...
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bioRxiv - Immunology 2022Quote: ... M1 cells were generated by addition of 20ng/mL of granulocyte-macrophage colony-stimulating factor (GM-CSF, Cat. No. 572904, BioLegend) and M2 macrophages with 20ng/mL of macrophage –colony-stimulating factor (M-CSF ...
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bioRxiv - Cancer Biology 2023Quote: ... 2×106 bone marrow cells were cultured at 37°C in 10ml bmDC culture medium (RPMI 1640 medium with 10% FBS, penicillin/streptomycin and 2-Mercaptoethanol) with 40ng/mL GM-CSF (BioLegend). At day 3 ...
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bioRxiv - Microbiology 2023Quote: ... BMDMs were stimulated for 2 hours prior to infection with vehicle or 10 ng/mL of each cytokine: GM- CSF (Biolegend), M-CSF (Miltenyi) ...
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bioRxiv - Immunology 2023Quote: ... in the presence of 40 ng/ml of granulocyte-macrophage colony-stimulating factor (GM-CSF) and IL-4 (both from Peprotech or Biolegend). Cells were cultured in 24-well plates in DMEM (Gibco) ...
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bioRxiv - Immunology 2023Quote: DCs were differentiated from bone marrow precursors obtained from naive BALB/c mice in the presence of murine GM-CSF (10ng/ml) and IL-4 (10ng/ml) both from Biolegend for 7 days ...
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bioRxiv - Microbiology 2024Quote: ... and where indicated containing 25 ng/mL human granulocyte-macrophage colony-stimulating factor (GM-CSF) ((# 572903, Biolegend, San Diego, CA) at 37 °C in a humidified chamber with 5% CO2 in 96-well ...
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bioRxiv - Immunology 2019Quote: ... Recombinant mouse CSF-2 (carrier free) was from BioLegend. L-α-phosphatidylcholine (PC ...
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bioRxiv - Microbiology 2022Quote: ... and 40 ng/ml mouse M-CSF (BioLegend 576406) and incubated for 7 days at 37°C with 5% CO2 ...
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bioRxiv - Microbiology 2020Quote: ... 5 × 105 cells/mL of CD14+ enriched cells were stimulated using recombinant human (rh) GM-CSF and rhIL-4 (BioLegend, USA) at a concentration of each 500 IU/mL in RPMI1640 media supplemented with 10% FCS at 37°C in 5% CO2 ...
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bioRxiv - Microbiology 2021Quote: ... were cultured in RPMI-1640 medium supplemented with 10% fetal bovine serum (FBS) in the presence of GM-CSF (20 ng/ml, BioLegend, Cat# 576304) for 9-12 days as described in (Dai et al. ...
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bioRxiv - Immunology 2023Quote: ... IL-1b, IL-6, IL-10, IL-17A, IL-12p70, GM-CSF, IL-23, IFN-b, MCP-1, IL-27; BioLegend Cat# 740446), Mouse TH Panel v4 (TNF-α ...
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bioRxiv - Cell Biology 2024Quote: ... and 2 x 106 cells were plated in 10 cm Petri-dishes with 10 ml of complete medium containing 10 ng / ml GM-CSF (BioLegend, Cat. # 576306). At day 3 and 6 fresh medium with GM-CSF was added ...
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bioRxiv - Cell Biology 2020Quote: ... were prepared from mT/mG mice as described with modifications.55 Ten million bone marrow cells/well were plated overnight in medium containing 20ng/ml murine GM-CSF(BioLegend, San Diego, CA), replenishing the medium daily for three days ...
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bioRxiv - Molecular Biology 2023Quote: ... and mouse anti-His (1:2500, 362601, BioLegend) were diluted in 5% BSA in 1× PBS + 0.2% Tween20 for blotting ...
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bioRxiv - Cancer Biology 2021Quote: ... and 20 ng/mL mouse macrophage colony stimulating factor (M-CSF) (BioLegend) for 8 days (66) ...
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bioRxiv - Biochemistry 2022Quote: ... Two microliters of mouse anti-hexa His antibody (BioLegend) were added to the 10 mL sample and incubated for 1 h at room temperature ...
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bioRxiv - Immunology 2022Quote: Mouse anti-His Tag antibody (clone J099B12, BioLegend #652502), goat anti-mouse IgG ...
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bioRxiv - Immunology 2021Quote: BMDMs were generated by culturing total BM cells for 7 days in complete RPMI supplemented with 10 ng/mL recombinant mouse M-CSF (BioLegend, Cat# 576406M-CSF). C8-S cells (ATCC ...
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bioRxiv - Molecular Biology 2020Quote: ... Purified anti-His tag mouse antibody was from BioLegend (USA). Secondary antibodies Alexa Fluor® 680 AffiniPure Donkey anti-mouse IgG and Alexa Fluor® 790 AffiniPure Donkey Anti-human IgG (H+L ...
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bioRxiv - Immunology 2023Quote: ... 5% M-CSF conditioned media or 50ng/mL M-CSF (Biolegend) (results did not differ) ...
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bioRxiv - Immunology 2021Quote: Sorted mMDSCs (50,000) were stimulated with 50 ng/ml recombinant mouse M-CSF (Biolegend) in Iscove’s Modified Dulbecco’s Medium (IMDM ...
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bioRxiv - Bioengineering 2022Quote: ... and 20 ng/mL recombinant mouse macrophage colony-stimulating factor (M-CSF, BioLegend, 576406). Successful differentiation was confirmed by flow cytometry staining with antibodies against macrophage markers ...
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bioRxiv - Cancer Biology 2022Quote: ... and 20 ng/mL recombinant mouse macrophage colony-stimulating factor (M-CSF, BioLegend 576406). 72 h after initial plating ...