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Citations for BioLegend :
401 - 450 of 1157 citations for C Reactive Protein Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2023Quote: ... samples were incubated with 20 μL of staining buffer containing 0.5 μL of TruStain FcX anti–mouse CD16/32 blocking agent (BioLegend 101319) for 30 min at room temperature ...
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bioRxiv - Immunology 2023Quote: ... then cut in half and transferred to Ce3D™ Permeabilization/Blocking Buffer (48 hr, room temperature, gentle shaking; 427706, Biolegend). The Ce3D™ Permeabilization/Blocking Buffer was discarded and replaced with Alexa Fluor 647 conjugated anti-mouse Iba-1 (1:100 ...
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bioRxiv - Immunology 2023Quote: All flow cytometry was performed at Fred Hutchison Cancer Center utilizing a 28-color panel focused on T cell phenotyping (Supplementary Table 1) [33] CVC and PBMC were incubated with Fc-blocking reagent (BioLegend) and fixable UV Blue Live/Dead reagent (ThermoFisher ...
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bioRxiv - Cancer Biology 2022Quote: ... Concentration of stable complexes following peptide exchange was quantified using the Flex-T HLA class I ELISA assay (Biolegend) per manufacturer’s instructions for HLA-A*02:01 ...
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bioRxiv - Microbiology 2023Quote: ... MBL) or H-2q-restricted LCMV NP118 peptide (MBL) and 50 U/mL of recombinant mouse IL-2 (BioLegend) for 1 h ...
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bioRxiv - Cancer Biology 2023Quote: ... MEFs were trypsinized and stained with Apotracker-Green (Apo-15 peptide) and 7-AAD according to manufacturer’s instructions (Biolegend) to detect apoptotic cells ...
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bioRxiv - Genomics 2024Quote: ... were plated in a 96 well V-bottom plate (200,000 cells/well) and 1ug/mL peptides added to restimulate cells along with Monensin and Brefeldin A (Biolegend). Iberdomide (10nM ...
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bioRxiv - Biophysics 2021Quote: ... This step was performed in order to break the existing cadherin junctions and allow the blocking antibodies (anti N-Cadherin antibody: LEAF™ Purified anti-human CD325, #350804, Biolegend, USA ...
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bioRxiv - Developmental Biology 2021Quote: ... ENR media was supplemented with 500 ng/ml rAspn and 1 μg/ml of ultra-LEAF™ CD44 function blocking antibody (Clone IM7; Biolegend) or equal amount of isotype control IgG2b kappa (Clone RTK4533 ...
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bioRxiv - Cancer Biology 2021Quote: ... resuspended in 100 µl of FACS buffer and incubated in 5 µl of Human TruStain FcX™ (Fc receptor blocking solution, BioLegend) for 10 minutes at RT ...
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bioRxiv - Cancer Biology 2020Quote: ... were treated with 10 Gy (as described above) and either PMX205 or vehicle and either IL-10 blocking antibody (LEAF Purified clone JES5-16E3, Biolegend #505012) (50 μg 24 hours before irradiation ...
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bioRxiv - Neuroscience 2022Quote: ... Primary immunoreaction solution consisted of blocking buffer that contained 1:1000 rabbit anti-melanopsin (ATS Bio AB N38) and 1:250 mouse anti-SMI32 (Biolegend 801701). After incubation in the primary immunoreaction solution ...
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bioRxiv - Cancer Biology 2022Quote: The following primary antibodies with indicated dilution in blocking buffer were used: Rabbit anti-Tuj1 (Covance, BioLegend, MRB-435P, 1:1,000), Mouse anti-Tuj1 (Covance ...
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bioRxiv - Immunology 2021Quote: ... Cells were stained for 10 minutes at room temperature with Ghost Dye Violet 510 and Fc receptor blocking solution (Human TruStain FcX™, BioLegend) and washed once in FACS buffer ...
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bioRxiv - Immunology 2021Quote: ... The frozen tissue sections were treated with blocking solution (5% normal donkey serum in PBS) and then stained with FITC-conjugated anti-Ly6G antibody (BioLegend, 1A8). Nuclei were stained by DAPI ...
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bioRxiv - Genomics 2021Quote: Both iMGs and oMGs were resuspended in staining buffer and were blocked with Fc receptor blocking antibody (Human TruStain FcX, BioLegend 422302) for 10 minutes ...
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bioRxiv - Immunology 2023Quote: ... Cells were pelleted at 500xg for 5 minutes and then resuspended in 50uL of PBS+ 2% HI-FBS + 2mM EDTA in the presence of Fc receptor blocking antibody (BioLegend, 101302). Cells were labelled with antibodies as described below ...
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bioRxiv - Immunology 2023Quote: ... Cells were stained with Zombie-violet or Zombie-NIR in PBS at 1:2000 dilution for 5 minutes at room temperature prior to resuspending in PBS + 2% HI-FBS + 2mM EDTA in the presence of Fc receptor blocking antibody (BioLegend, 101302) for blocking ...
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bioRxiv - Bioengineering 2023Quote: ... Successful blocking was confirmed by staining with anti-human CD44 antibody conjugated with Alexa Fluor 594 (clone: C44 Mab-5; Biolegend, USA) and quantified using the Cytek Aurora flow cytometer (4L 16V-14B-10YG-8R ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were incubated with primary anti-HA antibodies diluted in PBS-tween 0.2% blocking medium (mouse anti-HA, 1:5000, Biolegend cat. # 901513) over night at 4°C under agitation ...
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bioRxiv - Immunology 2023Quote: ... Cells were rinsed with FACS buffer (PBS containing 0.5% BSA and 2mM EDTA) for 3 min at 300g and incubated with Fc Receptor Blocking solution (Human TruStain FcX™, Biolegend) for 10 minutes prior to cell surface staining with fluorescently labeled antibodies in Brilliant Stain buffer (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... stained for 10 minutes at room temperature with Ghost Dye Violet 510 and Fc receptor blocking solution (Human TruStain FcX™, BioLegend), and washed once in FACS buffer ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed once with PBS supplemented with 1mM EDTA and resuspend in FcR blocking buffer using Human TruStain FcX (Biolegend, 422302) and Mouse TruStain FcX PLUS (Biolegend ...
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bioRxiv - Genetics 2021Quote: ... The cells were then incubated for 20 min at 4°C with the TotalSeq-C antibody mix: TotalSeq - C0048 anti-human CD45 Antibody (Biolegend; Cat# 368545), TotalSeq - C0103 anti-mouse/human CD45R/B220 (Biolegend ...
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bioRxiv - Cell Biology 2022Quote: ... recombinant proteins Serpin E2 (BioLegend, 769002) and Serpin A3 (R&D systems ...
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Mapping of the autophagosomal degradome identifies IL-7Rα as key cargo in proliferating CD4+ T-cellsbioRxiv - Immunology 2021Quote: ... γc (132307, BioLegend, manually conjugated with AF647, 1:100).
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bioRxiv - Biophysics 2019Quote: ... and HLA-A,B,C (Biolegend, clone W6/32) primarily conjugated antibodies ...
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bioRxiv - Immunology 2019Quote: ... overnight at 4°C in FOXP3 perm buffer (Biolegend). T-cells were analyzed with a BD LSRFortessa or FACSCanto II (BD Biosciences) ...
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bioRxiv - Biochemistry 2020Quote: ... CD117 (c-Kit) (Biolegend, clone 2B8, APC, 1:50), CD127 (IL-7Rα ...
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bioRxiv - Immunology 2020Quote: ... CD138 (281-2) and c-kit (2B8) from BioLegend.
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bioRxiv - Immunology 2021Quote: ... Furthermore a panel of 15 TotalSeq-C antibodies (BioLegend) was pooled ...
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bioRxiv - Immunology 2022Quote: ... 0.25ug total-seq C streptavidin-PE (C0951, Biolegend 405261), and 1ul total-seq C hashtag antibody (C0301-C0310 ...
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bioRxiv - Microbiology 2023Quote: ... DT-9 (anti-HLA-C, BioLegend, 373302, RRID AB_2650941), 3D12 (anti-HLA-E ...
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bioRxiv - Immunology 2022Quote: ... an individual TotalSeq Hashtag antibody (Biolegend, TotalSeq™-C) was added to the single-cell suspension of each mouse ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... anti-CD117 (c-Kit, BioLegend, San Diego, CA, USA), anti-61 (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... TotalSeq-C hashtag antibodies 1–12 (BioLegend, 1:100) and Spike and RBD antigen tetramers ...
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bioRxiv - Immunology 2023Quote: ... anti-CD117 (c-Kit)-PE (BioLegend, clone 2B8, #105808), anti-Ly-6A/E (Sca-1)-APC (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... and the fluorescence-conjugated antibodies: c-Kit (#105812, BioLegend), Sca-1 (#108126 ...
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bioRxiv - Cancer Biology 2024Quote: ... HLA-A/B/C clone W6/32 (Biolegend #311413), mouse MHC-I FITC conjugated H2-Dd/H-2Kd clone 34-1-2S (Invitrogen #11-5998-82) ...
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bioRxiv - Immunology 2024Quote: ... APC-anti-HLA-A/B/C (W6/32; BioLegend), PE-anti-CD4 (RPA-T4 ...
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bioRxiv - Cancer Biology 2024Quote: ... or HLA-A/B/C (BioLegend #311418, BioLegend #311406) for 30 minutes at 4 °C in PBS ...
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bioRxiv - Cancer Biology 2024Quote: ... or HLA-A/B/C (BioLegend #311418, BioLegend #311406) for 30 minutes at 4 °C in PBS ...
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bioRxiv - Bioengineering 2021Quote: ... using a modified version of the commercial protocol as previously described.7 Concentration of stable complexes following peptide exchange was quantified using the Flex-T HLA class I ELISA assay (BioLegend) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... biotinylated NP peptide loaded SLA monomers were freshly assembled into tetramer with streptavidin BV421 or BV650 (both from Biolegend, UK). Two million mononuclear cells were incubated with protease kinase inhibitor in PBS for 30 minutes at 37°C and tetramers added to the cells on ice for another 30 minutes ...
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bioRxiv - Immunology 2021Quote: ... cells were cultured in stimulation media without peptide stimulation or with phorbol 12-myristate 13-acetate (PMA) and ionomycin (BioLegend), respectively ...
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bioRxiv - Biochemistry 2020Quote: ... Traditional antibodies based labeling procedure was done by using c-Myc Antibody (9E10, Cat # 626801, BioLegend, USA; incubation 1 h at 4°C) and Anti-Mouse IgG (Fc specific)-FITC antibody produced in goat (Cat # F4143 ...
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bioRxiv - Immunology 2019Quote: After isolation cells were treated with Fc receptor blocking solution and stained with anti-CD14-APC (2 μg/ml, 61D3; Biolegend, San Diego, USA), anti-CD41-PE (2 μg/ml ...
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bioRxiv - Immunology 2020Quote: ... washed twice with cell staining buffer (0.5% bovine serum albumin/PBS) and incubated with 5 µl of human Fc receptor blocking solution (Trustain FcX, Biolegend, San Diego, CA) for 10 minutes at room temperature ...
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bioRxiv - Immunology 2020Quote: ... washed twice with cell staining buffer (0.5% bovine serum albumin/PBS) and incubated with 5 μl of human Fc receptor blocking solution (Trustain FcX, Biolegend, San Diego, CA) for 10 minutes at room temperature ...
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bioRxiv - Immunology 2020Quote: ... Cells were resuspended in 75µL of Fc blocking medium (5 µg/ml Purified anti-mouse CD16/32 Antibody, Biolegend in FACS buffer), and incubated for 20 minutes on ice ...