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Citations for BioLegend :
351 - 400 of 1157 citations for C Reactive Protein Blocking Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2023Quote: ... cells were washed with FACS buffer (PBS + 2% FBS + 0.05% NaN3) and incubated with Fc receptor blocking solution (Human TruStain FcX, BioLegend) for 5 minutes ...
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bioRxiv - Cell Biology 2022Quote: ... one million cells were first resuspended in 100 μL buffer containing 5 μL Fc Receptor Blocking Solution (Biolegend, #422302) and incubated for 15 minutes ...
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bioRxiv - Immunology 2023Quote: ... and single cell preparations were obtained by passing the cell mixture through a PluriStrainer (500 – 70µM cell strainers; 43-50500, PluriSelect) before blocking with Fc block for 10 min (422302, BioLegend). CD45+ cells were enriched using anti-CD45 magnetic MicroBeads and LS columns (130-045-801 and 130-042-401 ...
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bioRxiv - Bioengineering 2024Quote: ... Cells in each well were centrifuged at 350 g for 5 minutes and resuspended in 100 μL of PBS premixed with 0.5 μL of Trustain FcX plus blocking solution (BioLegend) on ice for 10 min ...
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bioRxiv - Cancer Biology 2024Quote: ... Barcoded cells were combined into a composite sample before incubation in Human TruStain FcX Fc Receptor Blocking Solution (BioLegend) for 10 min at room temperature ...
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bioRxiv - Developmental Biology 2024Quote: ... 45 µL of cell suspension was transferred to a new 1.5 mL Eppendorf tube (approximately 350,000 cells) and 5 µL of Human TruStain FcX™ Fc Blocking reagent (Biolegend) were added to block unspecific binding of the hashing antibodies ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were blocked overnight in Odyssey PBS Blocking Buffer (Li-Cor) and stained for 2 hours at RT with Syn1 (1:1,000; Biolegend) or γH2AX (1:1,000 ...
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bioRxiv - Immunology 2021Quote: 30 µL peptide-exchanged monomer formed in the above steps was mixed with 3.3 µL PE streptavidin (BioLegend) on a new plate and incubated on ice in the dark for 30 min ...
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bioRxiv - Cell Biology 2021Quote: ... Thrombin Receptor Activator Peptide-6 (TRAP-6; 30μM) or Adenosine Diphosphate (ADP; 30μM) along with CD42b-BV421 (1:60, Biolegend), PAC-1 FITC (1:12 ...
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bioRxiv - Immunology 2021Quote: Biotinylated DPB1*04:01-monomers loaded with TFEYVSQPFLMDLE peptide (S167-180) were tetramerised using PE-Streptavidin (Biolegend, 405204). 1.1 volume PE-conjugated streptavidin was added to 1 volume of DPB1*04:01-monomer (1 mg/mL) ...
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bioRxiv - Cell Biology 2021Quote: ... for 10 min at 4°C and stained with fluorophore conjugated antibodies for 20 min at 4°C (anti-Pdpn PE-Cy7, Biolegend 127411 ...
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bioRxiv - Cell Biology 2023Quote: ... then incubated for 30 minutes at 4°C with pre-chilled 4°C PBS (200 uL) containing 4 uL FITC-conjugated anti-CD63 (clone H5C6, Biolegend) and 4 uL APC conjugated anti-CD9 (clone H19a ...
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bioRxiv - Immunology 2024Quote: ... were isolated ex vivo from mouse peritoneal cavity by intraperitoneal lavage followed by magnetic bead-based enrichment for c-Kit+ cells using anti-CD117 (c-Kit)-APC antibody (Biolegend) and APC microbeads (Miltenyi Biotec) ...
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bioRxiv - Cancer Biology 2022Quote: ... and BV510 anti-mouse c-KIT (BioLegend,105839); T cell specific markers used were BV 421 PD-1 (BioLegend,135217) ...
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Inhibition of major histocompatibility complex-I antigen presentation by sarbecovirus ORF7a proteinsbioRxiv - Microbiology 2022Quote: ... C antibody conjugated to AF647 (W6/32, Biolegend). In some experiments ...
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bioRxiv - Immunology 2022Quote: ... anti-c-Kit-APC (BioLegend; 135108, 1 : 300), anti-CD1d-PE (Thermo Fisher Scientific ...
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bioRxiv - Molecular Biology 2019Quote: ... APC anti-mouse c-Kit (Biolegend, Cat# 105811), PE/Cy7 anti-mouse Gr1 (Biolegend ...
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bioRxiv - Molecular Biology 2020Quote: ... Anti-C/EBPβ antibody (Biolegend, Cat. no. 606202) was used at 1:2000 dilution for overnight at 4°C ...
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bioRxiv - Neuroscience 2020Quote: ... rabbit anti-Myh10 (C-terminus) antibody (Biolegend, 909901), rabbit anti-GFP antibody (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... BV421- conjugated c-kit (105827, Biolegend, clone 2B8), PE-Cy7-conjugated Ly-6A (122513 ...
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bioRxiv - Immunology 2021Quote: ... CD66a/c/e-A488 (clone ASL-32, Biolegend), CD326-BV650 (Clone 9C4 ...
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bioRxiv - Immunology 2022Quote: ... and anti-c-Kit FITC (Biolegend, clone 2B8). Cells were then washed into holding buffer (0.04% BSA in PBS) ...
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bioRxiv - Cancer Biology 2023Quote: ... and HLA-A,B,C-APC (BioLegend, #311410). Antibodies used for mouse studies ...
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bioRxiv - Immunology 2023Quote: ... and mouse anti-c-Myc (clone 9E10, Biolegend) were used as 1:300 ...
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bioRxiv - Cancer Biology 2021Quote: ... the cells were stained at the same time with the APC conjugated surface marker antibody CD45 after a 5 min blocking step with Human TruStain FcX solution (20x, BioLegend) diluted in 1 % BSA/PBS ...
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bioRxiv - Immunology 2021Quote: ... cells were subsequently blocked in flow buffer supplemented with either anti-mouse CD16/CD32 antibody (eBioscience) or Human TruStain FcX Fc Receptor Blocking Solution (BioLegend) for 30 min on ice ...
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bioRxiv - Immunology 2021Quote: ... flow buffer was supplemented either with anti-mouse CD16/CD32 antibody (eBioscience) or Human TruStain FcX Fc Receptor Blocking Solution (BioLegend). The following antibodies were used for flow analysis ...
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bioRxiv - Microbiology 2019Quote: ... Human B cells were incubated in human Fc receptor blocking antibody then stained with Zombie Aqua fixable viability dye (1:500 dilution) (BioLegend), according to manufacturer’s protocol ...
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bioRxiv - Neuroscience 2020Quote: For analysis of LILRB2 expression on marmoset and human monocyte derived macrophages were first blocked with human TruStain FcX Fc blocking solution (Biolegend), then labelled with and without anti-human LILRB2 (5 µg/mL ...
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bioRxiv - Neuroscience 2020Quote: ... Permeabilization was followed with blocking step by incubating the samples for 2 hr in PBS containing 3% normal goat serum (NGS, 927503, BioLegend) and 3% BSA and then stained overnight at 4°C with the primary monoclonal antibody (mouse anti-radixin ...
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bioRxiv - Neuroscience 2021Quote: ... Tissue samples were blocked/permeabilized using 10% normal goat serum in 0.3% Triton-X in PBS for 3 hours at room temperature followed by additional avidin biotin blocking using a kit according to manufacturer’s instructions (BioLegend, 927301). Primary immuno-staining was performed using antibodies ...
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bioRxiv - Microbiology 2021Quote: ... Cells were filtered through a 70 µm strainer and red blood cells were lysed before blocking FC receptors using anti-CD16/CD32 (BioLegend). Cell-specific proteins were labeled with the following antibodies ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were blocked for 10 min on ice following addition of 10 μL Fc blocking solution (BioLegend, cat. no. 422301), 4 μL of a 1% dextran sulfate solution (Research Products International ...
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bioRxiv - Cancer Biology 2022Quote: ... were added to the in vivo samples to obtain absolute counts of events acquired on the cytometer as well as using human and mouse Fc blocking antibodies (Biolegend) to reduce non-specific antibody staining ...
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bioRxiv - Neuroscience 2022Quote: ... Slices were then kept in PBS solution containing Triton X-100 and blocking agent (Normal Serum Block, CAT# 927502, Biolegend) for 2 h after which they were incubated in the solution containing ...
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bioRxiv - Immunology 2021Quote: ... Single cell suspensions were blocked with rat anti-mouse FcγIII/II receptor (CD16/CD32) blocking antibodies (“Fc-Block”) and stained with live/dead cell-exclusion dye (Zombie UV dye; Biolegend). The cells were then incubated with fluorophore-conjugated antibodies directed against cell surface antigens ...
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bioRxiv - Immunology 2020Quote: ... followed by incubation for 1h at RT in a 1:1000 dilution in blocking buffer of primary Ab (purified anti-Filaggrin polyclonal Ab, clone Poly19058, Biolegend). After washing ...
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bioRxiv - Microbiology 2022Quote: ... the BrdU-labeled splenocytes were surface stained for T-cell (CD3e-APC; eBioscience) marker after blocking with anti-mouse CD16/32 antibodies (BioLegend). Cells were then permeabilized and treated with DNase to expose BrdU epitopes followed by anti-BrdU-FITC and 7-AAD (7-amino-actinomycin D ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 million cells were resuspended in 1 mL of FACS buffer and 50 μL of Human TruStain FcX Fc Receptor Blocking Solution (BioLegend) was added and left to bind the cells on ice for 15 minutes ...
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bioRxiv - Immunology 2023Quote: ... Enriched cells were washed once in wash buffer and an Fc blocking step was performed using TruStain FcX (anti-mouse CD16/32) antibody (BioLegend) according to the manufacturer’s instruction.
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bioRxiv - Immunology 2023Quote: ... Cells were then washed in FACS stain buffer and resuspended in diluted Human TruStain FcX Fc blocking reagent according to manufacturer’s instructions (Biolegend, USA) at 4□°C for 10 minutes protected from light ...
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bioRxiv - Immunology 2023Quote: ... Samples were then split into 7 FACS tubes and incubated with 100uL of diluted Human TruStain FcX Fc blocking reagent according to manufacturer’s instructions (Biolegend, USA) at 4□°C for 10 minutes protected from light ...
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bioRxiv - Cell Biology 2023Quote: ... iMACs were then washed with PBS plus 2% FBS and incubated for 15 min at RT with 50 ul/mL Human TruStain FcX Blocking solution (BioLegend) in PBS + 2% FBS ...
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bioRxiv - Cell Biology 2023Quote: Flow cytometry of circulating leukocytes was performed by first blocking Fc receptors by incubating cells with TruStain FcX PLUS (anti-mouse CD16/32, Biolegend) for 10 minutes ...
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bioRxiv - Genomics 2023Quote: ... Cells were pre-incubated with 5 µl of Human TruStain FcX™ (Fc Receptor Blocking Solution, BioLegend Cat. No. 422301) per 100µl of cell suspension for 5–10 minutes at room temperature ...
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bioRxiv - Immunology 2023Quote: ... After blocking of free capture sites on the biosensors with an isotype control (10 µg/mL, human IgG1κ, clone QA16A12, BioLegend) for 600 s ...
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bioRxiv - Immunology 2023Quote: ... were resuspended in labeling buffer (1% BSA in PBS) and underwent Fc blocking for 10 minutes on ice using Human TruStain FcX (BioLegend). Next ...
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bioRxiv - Immunology 2023Quote: ... single cell suspensions were incubated on ice for 10 minutes with Fc receptor-blocking anti-CD16/32 (BioLegend Cat# 101301). Cell suspensions were first stained for 5 minutes at room temperature with LIVE/DEAD® Fixable Red Dead Cell Stain Kit (ThermoFisher Cat# L23102) ...
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bioRxiv - Cancer Biology 2023Quote: ... for dead cell exclusion followed by Fc-receptor blocking with purified mouse CD16/32 antibody (BioLegend, catalog #101301, clone 93). After cell surface marker staining with fluorescent-conjugated antibodies ...
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bioRxiv - Cell Biology 2024Quote: ... 2 mM EDTA in PBS) and incubated with Fc-blocking reagent (1:200, TruStain FcX, anti-mouse CD16/32, BioLegend). After washing ...