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Citations for BioLegend :
401 - 450 of 7197 citations for Adrenocorticotropic Hormone ACTH ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... cell-free supernatants were collected followed by ELISA to detect IL-10 and TGFβ using capture and detecting antibodies (Biolegend). Other M2 phenotype characteristics including the cell surface expressions of CD206 ...
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bioRxiv - Bioengineering 2021Quote: ... using a modified version of the commercial protocol as previously described.7 Concentration of stable complexes following peptide exchange was quantified using the Flex-T HLA class I ELISA assay (BioLegend) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... IFN-γ or GM-CSF release in the cell culture supernatant was measured using ELISA according to the manufacturer’s protocol (Sanquin and BioLegend, respectively).
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bioRxiv - Immunology 2021Quote: Organ homogenates were thawed and the resulting supernatants analyzed for cytokines and proteins by ELISA (Biolegend, BD, and R&D) and Luminex (R&D ...
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bioRxiv - Immunology 2020Quote: ... Culture supernatants were removed and analyzed for IL-2 production as a correlate for T cell activation using an ELISA MAX mouse IL-2 set (BioLegend). IL-2 values measured in supernatants from the wells containing BMDCs pulsed with proteins for different time periods were transformed into µM peptide equivalents by interpolating in a standard curve generated from the values obtained from hybridomas co-cultured with BMDCs at indicated peptide concentrations.
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bioRxiv - Immunology 2020Quote: ... The supernatants were collected and the amount of IL10 in the supernatants were measured using the Human IL10 ELISA MAX (Biolegend) kit according to the manufacturer’s protocol ...
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bioRxiv - Synthetic Biology 2021Quote: ... The samples were then flash frozen and defrosted for 4 rounds to lyse the cells and used on an IL-10 detection ELISA (Catalog 430601, Biolegend).
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bioRxiv - Immunology 2020Quote: ... Cytokine content of cell free supernatants was determined using standard sandwich ELISA according to the manufacturer’s instructions (IFN-γ and GM-CSF; Sanquin and Biolegend). Some target cells were pre-incubated for 2 days in the presence or absence of UGCG inhibitors.
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bioRxiv - Immunology 2022Quote: ... TNF and IL-2 in the supernatant of in vitro activated cells and murine IL-10 in the serum were determined using ELISA MAX Deluxe Set (BioLegend) according to manufacturer instructions.
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bioRxiv - Microbiology 2023Quote: ... Secreted levels of IFN-β were measured from BMDM supernatants twenty-four hours following the start of the infection following the Sandwich ELISA protocol published by BioLegend. Anti-IFN-β capture antibody (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... Cells were lysed in RLT buffer and supernatants were collected for analysis using ELISA (Total Tgf-β1 Legend MAX and Free Active Tgf-β1 Legend MAX, Biolegend). The limit of detection was 8 pg/mL for Tgf-β1.
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bioRxiv - Molecular Biology 2023Quote: ... Conditioned medium was collected and IL-1β levels were assessed with ELISA MAXTM Deluxe Set Mouse IL-1β (Biolegend, 432604) following manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... and GM-CSF (purified anti-mouse GM-CSF; biotin anti-mouse GM-CSF) ELISA antibodies and standards were purchased separately from Biolegend.
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bioRxiv - Immunology 2023Quote: ... Tgf-β concentration was measured using ELISA (Total Tgf-β1 Legend MAX and Free Active Tgf-β1 Legend MAX, Biolegend). The limit of detection was 8 pg/mL for Tgf-β1 ...
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bioRxiv - Immunology 2023Quote: ... Serum samples were assessed for titers of different antibody isotypes using LegendPlex Mouse Immunoglobulin Isotyping Panel and IgE ELISA (BioLegend) according to manufacturer’s instructions ...
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bioRxiv - Microbiology 2024Quote: ... TNF-α production was quantified in the supernatant of RAW 264.7 cells using a sandwich ELISA Max Deluxe Set (Biolegend) according to the manufacturers protocol.
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bioRxiv - Immunology 2022Quote: ... Cells were plated in a 24-well plate at a concentration of 500,000 cells/mL on either plate-bound α-CD3ε (2 μg/mL; 145-2C11; BioLegend) and soluble α-CD28 (1 μg/mL ...
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bioRxiv - Immunology 2024Quote: ... Mice CD4+ T cells (4x105/well in 96-well plate) were activated with plate-coated αCD3 (5 µg/ml, Biolegend) and αCD28 (10 µg/ml ...
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bioRxiv - Immunology 2019Quote: PBMCs were seeded in a 24 well plate at 2 × 106 cells/well in RPMI-1640 with anti-CD107a antibody (Biolegend, 1:200). Then ...
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bioRxiv - Bioengineering 2023Quote: ... T-cells that have been incubating with conditioned media were carefully transferred to the CD3-coated plates and further stimulated with 1 µg/mL anti-CD28 (BioLegend, clone: CD28.2) for another 48 hours ...
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bioRxiv - Microbiology 2023Quote: ... the plates were incubated with TMB substrate (BioLegend) for 15 minutes ...
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bioRxiv - Cell Biology 2021Quote: ... they were stained with Zombie Violet™ Fixable Viability Kit (1:1000, BioLegend) for 30 minutes on ice during secondary antibody staining ...
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bioRxiv - Developmental Biology 2022Quote: ... were incubated with Zombie Aqua™ Fixable Viability Kit (Biolegend, #423101, 1:1000) for 15 min at room temperature ...
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PRC1 directs PRC2-H3K27me3 deposition to shield adult spermatogonial stem cells from differentiationbioRxiv - Developmental Biology 2023Quote: ... PE/Cy7-conjugated anti-mouse CD117 (c-Kit) antibody (1:200, Biolegend, 105814) and FITC-conjugated anti-mouse CD9 antibody (1:500 ...
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bioRxiv - Cancer Biology 2023Quote: ... To exclude dead cells Zombie Aqua Fixable Viability Kit (#423102, 1:100, BioLegend) was added ...
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bioRxiv - Immunology 2022Quote: The concentration of IFN-γ was determined in the supernatant of 6×106 cells from each donor (6 healthy donors) using the Human IFN-γ ELISA deluxe set (BioLegend) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2020Quote: ... TNFα and IL-23 expression in the culture medium was examined using an ELISA (Invitrogen for TNFα Biolegend for IL-23).
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bioRxiv - Cancer Biology 2023Quote: ... and IL-2 were investigated with an enzyme-linked immunosorbent assay (ELISA MAXTM Deluxe Set, Biolegend®, San Diego, CA, USA) according to the manufacturer’s instruction ...
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bioRxiv - Immunology 2024Quote: ... that were pulsed with epitope (10 ng/mL) was collected and IFN-γ production was measured with an enzyme-linked immunosorbent assay (ELISA, BioLegend) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... NK cells from HD or MM patients were stimulated in 96 wells plates with plate bound anti-CD16 (clone 3G8 Biolegend, 10ug/ml) or with IL-12 (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... or co-stimulated with plate-bound CD3 (Biolegend #300333) and soluble CD28 (Biolegend #302943 ...
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bioRxiv - Cancer Biology 2019Quote: ... cells were removed from their plates with Accutase (BioLegend) and counted ...
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bioRxiv - Immunology 2021Quote: ... AMs were lifted from plates using Accutase™ (BioLegend) and seeded for experiments.
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bioRxiv - Cell Biology 2021Quote: ... Azide-Free (LEAF) plate-bound anti-mouse CD3 (Biolegend) was used at 2 μg/ml and soluble anti-mouse CD28 (Biolegend ...
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bioRxiv - Biochemistry 2022Quote: Flat-bottom 96 well microtitre plates (Nunc MaxiSorp, Biolegend) were coated overnight at 4°C with either r3CLpro-RBD chimer (2 μg/mL) ...
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bioRxiv - Biochemistry 2022Quote: Flat-bottom 96 well microtitre plates (Nunc MaxiSorp, Biolegend) were coated with r3CL-pro ...
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bioRxiv - Immunology 2022Quote: ... Plates were again washed and TMB substrate solution (BioLegend) was added for 15 minutes at room temperature prior to addition of stop solution (2M hydrochloric acid) ...
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bioRxiv - Immunology 2022Quote: ... Plates were washed and developed using TMB substrate (BioLegend). Reaction was stopped using 50μL of H2SO4 ...
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bioRxiv - Immunology 2022Quote: ... plate-bound antibodies (Abs) anti-NK1.1 (clone PK136; Biolegend), anti-NKG2D (clone A10 ...
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bioRxiv - Immunology 2023Quote: ... Plates were developed with 50 μl TMB (421101, BioLegend) and stopped using 50 μl 1.5 N H2SO4 ...
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bioRxiv - Immunology 2023Quote: ... stimulated with plate-bound anti-CD3 (Biolegend, 1μg/ml) and anti-CD28 (Biolegend ...
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bioRxiv - Cancer Biology 2020Quote: ... 2 mM EDTA) and stained with Zombie Aqua Fixable Viability Kit (BioLegend, 1:200). Living ...
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bioRxiv - Immunology 2021Quote: ... fixed for 1 h at 25 °C using the True Nuclear fixation kit (BioLegend), washed twice with True Nuclear Permeabilization/Wash buffer ...
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bioRxiv - Immunology 2022Quote: ... fixed for 1 h at 25°C using the True Nuclear fixation kit (BioLegend), washed twice with True Nuclear Permeabilization/Wash buffer ...
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bioRxiv - Microbiology 2023Quote: ... Cytokine levels were determined using the human inflammation panel 1 LEGENDplex™ kit (Biolegend) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... resuspended in 1 ml of PBS containing 1 µl live/dead stain (Zombie UV Fixable Viability kit (Biolegend, cat. no. 423107)) and incubated on ice for 30 min ...
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bioRxiv - Immunology 2022Quote: Supernatants were assessed for cytokine production using the LEGENDplex™ Human Inflammation Panel 1 (13-plex) in a V-bottom plate per the manufacturer’s instructions (BioLegend, San Diego, CA, USA). Flow cytometry data were acquired on a BD Fortessa analyzer (BD Biosciences ...
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bioRxiv - Biophysics 2023Quote: ... were isolated from buffy coats by density gradient centrifugation (Lymphoprep, StemCell ref: 07851) and activated on plates coated with anti-CD3 (1 mg/ml, Biolegend, ref: 317347, clone: OKT3) and anti-CD28 (1 mg/ml ...
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bioRxiv - Immunology 2020Quote: ... Culture supernatants thereof were removed and analyzed for IL-2 production as a correlate for T-cell activation using an ELISA MAX mouse IL-2 set (BioLegend, SanDiego, USA).
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bioRxiv - Immunology 2020Quote: ... The cytokine production was measured using the ELISA Deluxe Set Human IL-8/CCL20/IL-1β (Biolegend, San Diego, California, United States), following the manufacturer’s protocol.