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Citations for BioLegend :
601 - 650 of 7197 citations for Adrenocorticotropic Hormone ACTH ELISA Kit 1 Plate since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2023Quote: ... permeabilized (BioLegend cytokine staining kit) and stained with anti-cytokine antibodies.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... Cytokine levels in macaque plasma were measured using the LEGENDplex NHP Inflammation Panel in filter plates (BioLegend #740332). Plasma samples were diluted 1:4 in assay buffer ...
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bioRxiv - Immunology 2022Quote: ... All cells were differentiated for 48 hours in the presence of plate-bound αCD3 (2.5ug/ml; Biolegend, #100339), rIL-6 (20 ng/ml ...
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bioRxiv - Immunology 2019Quote: Cells were lifted off culture plates with 1X PBS + EDTA (BMDMs, RAW 264.7 and microglia) or Accutase (Biolegend) (MEFs and astrocytes) ...
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bioRxiv - Immunology 2019Quote: Polarized CD4+ effector T cells were generated by activating purified HD naïve CD4 T cells with plate-bound anti-CD3 (UCHT1) and anti-CD28 (CD28.2) (both 5μg/ml, Biolegend) in the presence of IL-2 (50U/ml) ...
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bioRxiv - Immunology 2021Quote: ... plates were coated with CD3 antibody (clone OKT3 at 0.5μg/mL) purchased from BioLegend (San Diego, CA USA) 24 hours prior to start of experiment ...
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bioRxiv - Microbiology 2021Quote: ... primary CD4+T cells were activated for 4 days with 1μg/ml plate-bound αCD3 antibody (cloneOKT3, Biolegend) in the presence of 2 μg/mL soluble αCD28 antibody(clone CD28.2 ...
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bioRxiv - Immunology 2022Quote: ... 5x105 cells were plated per well of a 48-well plate coated with CD3 (5 µg/ml) (Biolegend) and cultured in regular T cell media (RPMI 1640 supplemented with 10% heat inactivated FBS (Thermo Fisher Scientific) ...
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bioRxiv - Immunology 2020Quote: ... according to the manufacturer’s protocol and activated in vitro using a combination of plate bound anti-CD3 (2μg/mL) (BioLegend) and soluble anti-CD28 (2μg/mL ...
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bioRxiv - Immunology 2020Quote: ... Cells were placed in V-bottom plates and stimulated with 500X Cell Activation Cocktail with Brefeldin A (Biolegend) in IMDM medium supplemented with 8% FCS ...
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bioRxiv - Immunology 2020Quote: ... 2.5 × 105 CTV-labelled T-cells were stimulated with 5µg/ml of plate-bound anti-CD3 (300331, Biolegend) and 5µg/ml of soluble anti-CD28 (302913 ...
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bioRxiv - Immunology 2022Quote: ... activated with SIINFEKL (63ng/ml - 500ng/ml) or plate-bound anti-CD3 (1μg ml, clone 145-2C11, Biolegend) and anti-CD28 (1μg/ml ...
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bioRxiv - Immunology 2022Quote: ... Single-cell suspensions of rested PBMCs were aliquoted into plates pre-coated with anti-CD3 (clone : UCHT1, Biolegend) and anti-CD28 (Clone:CD28.2 ...
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bioRxiv - Microbiology 2022Quote: Nunc 96-well maxisorp plates (Thermo) were coated overnight at 4°C with 100 ng of FcγRI (Biolegend) in coating buffer (0.1M sodium bicarbonate ...
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bioRxiv - Cancer Biology 2022Quote: ... from healthy human donor Leukopaks and expanded either on anti-CD3 coated plates (+anti-CD28/IL-2, BioLegend), or in the presence of matched donor-derived monocyte-derived dendritic cells (Wölfl and Greenberg ...
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bioRxiv - Immunology 2023Quote: ... Cells were plated in a 96-well round-bottom plate coated with 3 μg/ml anti-CD3 (Biolegend), uncoated well or with PMA (1 ng/ml ...
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bioRxiv - Immunology 2023Quote: ... The plates were washed and detection antibodies (biotinylated mouse anti-IgG1, anti-IgG2a or anti-IgE from BioLegend) were added and incubated at room temperature for 1 hour ...
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bioRxiv - Immunology 2023Quote: ... 5×106 splenocytes were added to each well of a 24-well plate and stimulated with anti-CD3 and -CD28 mAb (1μg/ml and 5μg/ml, respectively; Biolegend) for IFN-γ production or LPS (1μg/ml ...
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bioRxiv - Cancer Biology 2024Quote: Pre-coat a 12-well plate overnight at 4 °C using CD3 antibody (5 μg/ml, 317326, Biolegend), and before inoculation ...
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bioRxiv - Developmental Biology 2021Quote: ... and Sca1-AF647 (Biolegend, clone D7, 1 µg ml−1, 1:500). Cells were then stained with streptavidin-APC or Streptavidin-PE/Cy7 (BD-Biosciences ...
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bioRxiv - Cancer Biology 2023Quote: ... CD49f-PE/Cy7 (Biolegend, clone GoH3, 1 µg ml−1, 1:200). DAPI was used to detect dead cells ...
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bioRxiv - Immunology 2022Quote: ... Sca-1-PerCP-Cy5.5 (Biolegend, 1:1,000), CD45-APC-Cy7 (Biolegend,1:200) ...
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bioRxiv - Physiology 2022Quote: ... Mac-1 and Gr-1 (Biolegend, 133307), CD4 (Biolegend ...
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bioRxiv - Microbiology 2023Quote: ... PD-1(Biolegend, RMP1-30, 1:200), KLRG1(Biolegend ...
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bioRxiv - Cell Biology 2024Quote: ... Icam-1 Antibody (Biolegend, 116101, 1:60), Anti-Cytokeratin19 (Abcam ...
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bioRxiv - Immunology 2021Quote: ... and transferred to round-bottom 96-well plates for staining with Aqua live/dead stain (Life Technology or Biolegend), followed by Fc receptor blocking (normal immunoglobulin ...
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bioRxiv - Immunology 2022Quote: ... followed by stimulation with plate bound anti-CD3 (2 μg/ml) and anti-ICOS (5 μg/ml; C398.4A; Biolegend) for 24 hours.
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bioRxiv - Immunology 2022Quote: ... CAR T cells were incubated for 24 hours in flat bottom 96 well plates that were pre-coated overnight with 10 μg/mL of purified anti-CD244 (clone C1.7, BioLegend), CD2 (clone LT2 ...
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bioRxiv - Microbiology 2020Quote: ... cells were counted and plated on 6 well plates in RPMI + 5% FBS + 20 ng/ml GM-CSF (BioLegend). Cells were maintained at 37° C 5% CO2 and supplemented on day 3 ...
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bioRxiv - Immunology 2020Quote: ... Th1 cells were differentiated for 4 days in the presence of plate-bound αCD3 (5 μg/ml; Biolegend, #100339), rIL-12 (25 ng/ml ...
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bioRxiv - Immunology 2021Quote: ... 1×106 splenocytes were isolated from mice and cultured on Immulon U-bottom plates coated with antibodies for Ly49D (0.5mg/ml) (Biolegend) or NKp46 (1mg/ml ...
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bioRxiv - Immunology 2020Quote: ... naïve CD4 T cell were activated in vitro using a combination of plate bound anti-CD3 (2μg/mL) (BioLegend) and soluble anti-CD28 (2μg/mL ...
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bioRxiv - Immunology 2020Quote: ... naïve CD4 T cells (106 cells/mL) were activated in vitro using a combination of plate bound anti-CD3 (2μg/mL) (BioLegend) and soluble anti-CD28 (2μg/mL ...
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bioRxiv - Immunology 2022Quote: ... CD69-) from OT-I mice were stimulated with plate-bound anti-CD3 mAb (5µg/ml, clone 145-2C11, Biolegend), and soluble anti-CD28 (1µg/ml ...
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bioRxiv - Cancer Biology 2022Quote: Cells cultured in 2D were seeded at 5 × 105 cells/well in 6-well plates and incubated with recombinant human interferon gamma (IFNγ) (0.5 ng/mL) (Biolegend) and/or recombinant tumour necrosis factor alpha (TNFα ...
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bioRxiv - Immunology 2022Quote: ... Cell cultures were stimulated for 3 days with either 100 ng/mL soluble or 1.25 μg/mL immobilised (plate-bound) anti-CD3 mAb (clone OKT3, BioLegend) in combination with 1 μg/mL soluble anti-CD28 mAb (clone CD28.2 ...
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bioRxiv - Immunology 2022Quote: Plates (48- or 96-well, flat bottom) were coated with 10 μg/ml αCD3ε mAb (BioLegend, San Diego, CA) and incubated at 37°C for 5 hours ...
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bioRxiv - Immunology 2023Quote: ... PBMCs were seeded in 96-well plates and cultured alone or stimulated with anti-CD3 antibody (Clone: OKT3, Biolegend) or treated with 50 ng/ml IL-15 for 4 days ...
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bioRxiv - Immunology 2023Quote: ... 130-120-662) for 3 days in 24-well plates coated with 3μg/ml anti-CD3 (145-2c11; Biolegend) and 3μg/ml anti-CD28 (37.51 ...
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bioRxiv - Molecular Biology 2023Quote: ... Penicillin-Streptomycin and IL-2 (100 IU/ml) and activated for 24 h in the presence of plate-bound anti-CD3 antibody (2.5 µg/mL; BioLegend) and soluble anti-CD28 antibody (2.5 µg/mL ...
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bioRxiv - Immunology 2023Quote: ... A 96 well plate was pre-incubated with a 5 µg/ml solution of anti-mouse CD3e (100314, Biolegend) in sterile PBS at 37°C for 2 h ...
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bioRxiv - Immunology 2023Quote: Activation consisted of 10μg/mL plate-bound anti-human CD3 antibody (300437) and 1μg/mL soluble anti-human CD28 antibody (302902) purchased from BioLegend, USA ...
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bioRxiv - Immunology 2023Quote: ... Activation consisted of 5μg/mL plate-bound anti-mouse CD3ε antibody (100340) and 5μg/mL soluble anti-mouse CD28 antibody (102116) purchased from BioLegend, USA ...
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bioRxiv - Immunology 2023Quote: ... 3x105 sorted naïve CD4 T cells were stimulated with plate-bound anti-CD3 (145-2C11, Cytek) (5 μg/ml) and soluble anti-CD28 (clone 37.51, Biolegend) (1 μg/ml ...
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bioRxiv - Biochemistry 2024Quote: ... the BMMs were seeded into 6-well plates and incubated with macrophage colony-stimulating factor (M-CSF) (20_ng/ml, Biolegend) and Receptor activator of nuclear factor kappa-Β ligand (RANKL ...
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bioRxiv - Cell Biology 2024Quote: ... were added to the isolated lymphocyte suspension before seeding at 1mL/well in a 24-well plates coated with 1µg/mL of anti-CD3ε (100340, Biolegend) 24 h before the procedure.
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bioRxiv - Genomics 2024Quote: ... were plated in a 96 well V-bottom plate (200,000 cells/well) and 1ug/mL peptides added to restimulate cells along with Monensin and Brefeldin A (Biolegend). Iberdomide (10nM ...
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bioRxiv - Immunology 2024Quote: ... cells were removed by washing and the plates were washed and probed with biotin α-IFNγ (clone XMG1.2, Biolegend) followed by incubation with Streptavidin-ALP (Mabtech) ...
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bioRxiv - Immunology 2024Quote: ... 8×105 naïve CD8+ or CD4+ T cells have been stimulated with 10µg/ml anti-CD3 (plate-coated) (Biolegend 300465) and 2µg/ml anti-CD28 (Biolegend 302943 ...
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bioRxiv - Cancer Biology 2022Quote: ... CD106 (1:100, MVCAM.A, #105717), CD62L (1:200, MEL-14, #104435), PD-1 (1:100, 29F.1A12, #135215) (Biolegend); Streptavidin (1:200 ...