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Citations for BioLegend :
401 - 450 of 909 citations for 6 4 MORPHOLINYL PYRAZINAMINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2022Quote: ... and stained (20 min, 4°C) with B220-PerCP/Cyanine5.5 (#103235; BioLegend) and CD138-PE (#142504 ...
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bioRxiv - Cancer Biology 2023Quote: ... PerCP/Cyanine5.5 anti-mouse CD4 Antibody (BioLegend, catalog #116011, clone RM4-4), PE/Cyanine7 anti-mouse CD8α Antibody (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... Standards for both IL-4 and IL-13 were purchased from BioLegend. IL-10 ELISA kit was purchased from BioLegend
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bioRxiv - Immunology 2023Quote: ... and stained for 30mins at 4°C with: TruStain FcX (Biolegend #422302), CD45-BV421 (clone HI30 ...
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bioRxiv - Immunology 2023Quote: ... and stained for 30mins at 4°C with: TruStain FcX (Biolegend #422302), CD45-AF647 (clone QA17A19 ...
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bioRxiv - Neuroscience 2024Quote: ... The primary incubation was performed overnight at 4°C (phospho-Ser129, BioLegend Cat # 825701 ...
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bioRxiv - Immunology 2024Quote: ... and 10 μg/mL anti-mouse IL-4 antibody (clone 11B11, BioLegend)) or Th2-polarizing condition (10 ng/mL recombinant mouse IL-4 (#574304 ...
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bioRxiv - Immunology 2024Quote: ... CD4 (clone RM4-4) and CD8 (clone 53-6.7) were from Biolegend. Cleaved caspase 3-AF647 (Clone D3E9 ...
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bioRxiv - Cancer Biology 2021Quote: NK cells were isolated from C57BL/6 mice (male and female) with the MojoSort mouse NK cell isolation kit (BioLegend) (purity >85 % based on the flow cytometric analysis with NK1.1 and CD49b antibodies) ...
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bioRxiv - Cancer Biology 2021Quote: ... The following anti-mouse antibodies we used for surface staining: Alexa Fluor 647-labeled H-2Kb/H-2Db – MHC Class I (clone 28-8-6, Biolegend); PE-labeled I-A/I-E – MHC Class II (Clone M5/114.15.2 ...
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bioRxiv - Immunology 2022Quote: ... Every 10^6 B cells in 100 μL were then stained with 2.5 μL FITC anti-human CD19 antibody (BioLegend, 392508), 2.5 μL FITC anti-human CD20 antibody (BioLegend ...
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bioRxiv - Immunology 2020Quote: ... Phenotypes of B cells before and after 6 days of culture were determined by flow cytometric analysis using mouse monoclonal antibody clones: CD19 PECy7 HIB19 (Biolegend), CD27 BV421 O323 (Biolegend) ...
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bioRxiv - Genomics 2020Quote: ... 1.25 × 10^6 PBMCs were spun down and resuspended in 25 μl CITEseq antibody cocktail (TotalSeq™-A Antibodies, BioLegend) containing among others one hashing antibody per each sample ...
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bioRxiv - Microbiology 2021Quote: ... prior to staining cells in 100 µL perm/wash with 0.5 µL APC anti-human IL-6 antibody (Cat# 501112, Biolegend®) overnight at 4°C ...
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bioRxiv - Immunology 2023Quote: ... with recombinant IL-15/IL-15RA complex (100 ng/ml; Thermo Fischer, Waltham, MA) or recombinant mouse IL-6 (50 ng/ml, BioLegend), TGF- β1 (1ng/mL) ...
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bioRxiv - Immunology 2023Quote: ... Single cell suspensions were then tagged with mouse Total-Seq anti-mouse hashtags C0306 (Brafi: Hashtag 6) and C0307 (Brafi + C-Me: Hashtag 7) (BioLegend) according to manufacturers’ protocols.
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Mice generated with induced pluripotent stem cells derived from mucosal-associated invariant T cellsbioRxiv - Immunology 2023Quote: ... CD3+ T cells were purified from C57BL/6 spleen cells with MojoSort mouse CD3 T cell isolation kit (Biolegend, USA), while CD8+T cells were isolated by adding biotin-labeled anti-CD4 antibody into MojoSort mouse CD3 T cell isolation kit ...
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bioRxiv - Immunology 2023Quote: BMMCs were generated from the BM cells of C57BL/6 mice (Japan SLC, Hamamatsu, Japan) by cultivation in the presence of 5 ng/mL of mouse IL-3 (BioLegend) as previously described 18 ...
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bioRxiv - Physiology 2023Quote: ... IL-6 and IL-10 in the homogenates were measured using LEGENDplexTM Mouse Custom Panel (BioLegend, San Diego, CA, USA). Assay was performed in the Attune NxT flow cytometer (Thermo Fisher) ...
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bioRxiv - Immunology 2023Quote: ... from uninfected or MCMV-infected WT C57BL/6 mice were sorted as described and stained with barcoded antibodies (Total-Seq B, BioLegend). After hash-staining ...
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bioRxiv - Cancer Biology 2023Quote: 1 × 106 target cells (B16-F10 and Hepa1-6) were suspended in 1ml DPBS containing 5 μM Calcein-AM (BioLegend) and incubated at 37℃ for 30 minutes in the dark ...
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bioRxiv - Immunology 2024Quote: ... Flow cytometry analysis of CTLA4-mediated trans-endocytosis was performed as previously described [6] using the True-NuclearTM Transcription Factor Buffer Set (BioLegend) and the following intracellular antibody panel ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies were applied overnight at 4°C and included SMI31 (Biolegend, 801601), IBA1 (Wako ...
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bioRxiv - Neuroscience 2019Quote: ... were incubated with a combination of 4 µg Tau12 (Biolegend, cat no 8065), 4 µg Tau 5 (Abcam ...
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bioRxiv - Immunology 2019Quote: Neutrophils obtained after 4-day differentiation were washed and labeled using CFSE (BioLegend). Delta T dishes (Bioptech ...
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bioRxiv - Immunology 2021Quote: ... MDM were suspended in 100μL of 4°C annexin V binding buffer (BioLegend) and the two pHrodo loaded sets were stained with 2μL of FITC Annexin V (BioLegend) ...
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bioRxiv - Neuroscience 2021Quote: ... Cells were then fixed in 4% paraformaldehyde and permeabilized using Permeabilization buffer (Biolegend). Intracellular proteins were stained with antibodies against TNF (MP6-XT22 ...
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bioRxiv - Cancer Biology 2020Quote: ... Samples were incubated overnight at 4°C in primary antibody mixtures (HIF1α, Biolegend; EZH2 ...
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bioRxiv - Cancer Biology 2022Quote: ... and incubation with Human TruStain FxC (10 minutes at 4°C; Biolegend, #422302) to block Fc receptor-mediated binding ...
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bioRxiv - Immunology 2020Quote: ... and cultured for 4 days +/- αIL10 antibody +/- isotype control (5μg/ml, both Biolegend). Assessment of the direct effect of IL-10 on human CD4 T cells was performed by plate bound αCD3/28 (1μg/ml ...
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bioRxiv - Neuroscience 2020Quote: ... Remaining volume was incubated at 4°C with anti-HA antibody (Biolegend, #901513) at 1:150 dilution for 4hrs on a gentle spinner ...
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bioRxiv - Immunology 2020Quote: ... cells were fixed for 10 min at 4°C using IC fix (Biolegend) and cells were then incubated in for 20 min at RT in Permeabilization buffer (biolegend) ...
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bioRxiv - Cancer Biology 2022Quote: ... we added 4 μg/ml PE-labeled anti-CD63 antibody (BioLegend, clone H5C6). We imaged the nanowell using Zeiss Axio Observer Z1 microscope equipped with 20x/0.8 NA objectives and a Hamamatsu Orca Flash v2 camera.
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bioRxiv - Developmental Biology 2023Quote: ... additional staining with anti-CD34 (4 µL CD34 APC/Cy7, clone: 581, Biolegend) and anti-CD14 antibodies (3 µL CD14 PE-CF594 ...
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bioRxiv - Immunology 2023Quote: ... Cells were then washed and stained with CTLA-4-PE-Dazzle 594 (Biolegend), Ki67-PerCP-eFluor 710 (Invitrogen) ...
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bioRxiv - Immunology 2023Quote: ... 4 µL of cocktail containing PMA/ionomycin + Brefeldin A (Biolegend, San Diego, CA) was added ...
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bioRxiv - Neuroscience 2024Quote: ... at 4°C for 48 hrs: goat anti-mouse Alexa Fluor 488 (Biolegend), goat anti-rabbit Alexa Fluor 594 (Abcam) ...
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bioRxiv - Immunology 2024Quote: ... or Th2-polarizing condition (10 ng/mL recombinant mouse IL-4 (#574304, BioLegend), and 10 μg/mL anti-mouse IL-12/23p40 antibody (clone C17.8 ...
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bioRxiv - Developmental Biology 2023Quote: ... Embryos were then incubated overnight at 4 °C with Epcam (cat#118202, Biolegend) (1:100 ...
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bioRxiv - Cancer Biology 2019Quote: ... at concentration 2×10^6/ml in IMDM containing 10% FBS and 100U/ml of recombinant mouse IL-2 (BioLegend, CA). Cells were cultured for up to 7 days and used for functional experiments in vitro and in vivo.
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bioRxiv - Microbiology 2021Quote: ... and resuspended in RPMI with 10% FBS medium and were stimulated for 6 h with a cell activation cocktail containing PMA and ionomycin (Biolegend, # 423302) in the presence of protein transport inhibitor ...
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bioRxiv - Immunology 2022Quote: ... Every 10^6 B cells in 100 μL solution were then stained with 3 μL FITC anti-human CD20 antibody (BioLegend, 302304), 3.5 μL Brilliant Violet 421 anti-human CD27 antibody (BioLegend ...
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bioRxiv - Immunology 2020Quote: Supernatants of stimulated T cells were harvested on day 6 of culture and analyzed using the LEGENDplex™ human Thelper 1/2/9/17 multiplex kit (BioLegend). Beads were acquired on a BD Canto II (BD Bioscience ...
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bioRxiv - Developmental Biology 2021Quote: ... Surface staining was done by adding the flow cytometry antibody cocktail outlined in Extended Data Table 6 containing F4/80 (BioLegend, 123120), CD11b (BioLegend ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were induced to differentiate by culturing in the absence of β-Estradiol for 6 days before cells were stained for CD11b (Biolegend) and analyzed on the LSRII flow cytometer (BD Biosciences) ...
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bioRxiv - Microbiology 2023Quote: ... and subsequently with biotinylated α- CADM1 antibody (MBL, Cat. No. CM004-6) and streptavidin-conjugated secondary antibody (BioLegend, Cat. No. 405226). Cells were resuspended in 4◦C PBS 2% FCS before cell sorting.
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bioRxiv - Physiology 2023Quote: The levels of TNF-α and IL-6 in the serum and the supernatant of the crushed muscle tissues were measured using a commercial kit (mouse TNF-α and IL-6 ELISA max standard set, BioLegend). The values of TA and GA muscles were revised to the protein concentration measured by BCA kit and expressed as pg/mg.
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bioRxiv - Cancer Biology 2023Quote: For inflammatory activation colon fibroblasts were plated in 6-wells and after 24 hours treated with 1 ng/ml murine recombinant IL-1α (Biolegend, 575002) in PBS for the indicated time periods ...
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bioRxiv - Cancer Biology 2023Quote: Plasma Ig were analysed using the LEGENDplex Mouse Immunoglobulin Isotyping panel (6-plex) by flow cytometry (Cat #740493, BioLegend, CA, USA). Plasma sample and standards was prepared using assay buffer as per the manufacturer’s instruction ...
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bioRxiv - Immunology 2023Quote: ... CD3+ T cells were isolated from spleens of 6-to-8-week-old OT-II mice by MojoSort mouse CD3+ T cell isolation kit (Biolegend 480024). Stimulated FLS were washed with PBS and co-cultured with CD3+ T cells at a concentration of 5-10×105 cells/mL in 10% RPMI for 5 days with either the cognate OVA323-339 antigen (2μg/mL ...