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Citations for BioLegend :
501 - 550 of 909 citations for 6 4 MORPHOLINYL PYRAZINAMINE since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: Whole PBMCs were seeded at 1-2×10^6 cells per well in 48-well plates in the presence of 200ng/ml soluble anti-CD3 (OKT3 clone, Biolegend, San Diego, CA) and 10ng/ml IL-2 (Peprotech ...
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bioRxiv - Immunology 2020Quote: ... DCs were isolated from spleens and lymph nodes of C57BL/6 mice using a MojoSort™ Mouse Pan Dendritic Cell Isolation Kit (BioLegend cat. 480097). DCs were cultured with supernatants of necrotic keratinocytes generated by an overnight culture of UV-irradiated (2 J/m2/s for 40 min ...
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bioRxiv - Immunology 2020Quote: Concentrations of IL-1β and IL-6 in cell culture supernatants or blood serum were measured using cytokine-specific ELISAs (BioLegend ELISA Max kits), according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2022Quote: ... unpulsed splenocytes and the S-peptide-pulsed splenocytes were stained with 0.5 μM and 5 μM (respectively) of 5-(and 6-) carboxyfluorescein diacetate succinimidyl ester (CFSE; BioLegend, San Diego, CA, USA). The unpulsed and peptide-pulsed splenocytes were mixed at a 1:1 ratio (5 × 106 cells each) ...
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bioRxiv - Immunology 2021Quote: Serum samples were collected three hours after the first STING agonist injection and analyzed for inflammatory cytokines (IFN-α, IFN-ß, TNF-α and IL-6) using a LEGENDplex Mouse Inflammation Panel (BioLegend). For cell culture supernatants ...
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bioRxiv - Cancer Biology 2022Quote: CAR-T cells were co-cultured with tumor at ratio of 2:1 for 6 hours at 37o C in CO2 incubator with CD107a antibody (Biolegend, 328620, clone-H4A3) and golgistop (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: Purified and biotinylated IL-6 (504502 and 504601, respectively) and IL-10 (505002 and 504906, respectively) antibodies were purchased from Biolegend (San Diego, CA). Purified antibodies were used to coat an ELISA plate ...
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bioRxiv - Immunology 2024Quote: NK cells were enriched from splenocytes from WT or Pvrig KO C57BL/6 mice by using the MojoSort™ Mouse NK Cell Isolation Kit (Biolegend, cat. 480049). Enriched NK cells were cultured and stimulated in vitro in RPMI-1640 Medium (ATCC ...
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bioRxiv - Pharmacology and Toxicology 2024Quote: Determination of the cytokine levels in the supernatants was carried out using commercial ELISA kits for human IL-6 and TNF (BioLegend, San Diego, US). ELISA was carried out according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2022Quote: ... To measure the cytokines/chemokines present in the SN we chose the LEGENDplex™ bead-based immunoassay and quantified the following cytokines/chemokines from the panels COVID-19 Cytokine Storm 1 (CCL2, CCL5, CXCL10, IFN-γ, IL-10, IL-6; Biolegend cat. No. 741088) and Human Neurodegeneration Biomarkers 1 (NFL ...
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The loss-of-function of SOCS2 increases the inflammatory response to Staphylococcus aureus infectionbioRxiv - Immunology 2023Quote: ... Individual cytokine detection kits were also used to quantify mouse IL-6 and TNF-α (Bio-techne, France) and GM-CSF (Biolegend, San Diego, USA).
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bioRxiv - Immunology 2021Quote: ... prior to staining for 1 h at 4°C with the following antibodies from BioLegend (most) or Novus Biologicals (MARCO ...
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bioRxiv - Immunology 2021Quote: ... cells were incubated overnight at 4 °C with the primary antibodies against ASC (HASC-71, BioLegend). Cells were washed 3 times with washing buffer (1x PBS and 0.025% of Tween20 ...
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bioRxiv - Genomics 2019Quote: ... cells were stained in batches of 1×107 cells for 30 min at 4°C (BioLegend: CD46 clone TRA-2-10 #352405 - 3μl per 1×106 cells ...
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bioRxiv - Immunology 2019Quote: Neutrophils obtained after 4-day differentiation (“in vitro-derived”) were washed and labeled using CFSE (BioLegend). Murine bone marrow neutrophils were isolated by negative selection (StemCell Technologies ...
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bioRxiv - Genomics 2021Quote: ... This was followed by 30min incubation at 4 °C with a Streptavidin-PE (Biolegend, 1:5000), anti c-kit-APC (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... After 4 hours BMDMs were harvested and stained with an antibody against F4/80 (BM8, Biolegend) and propidium iodide (BD Biosciences) ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... cell surfaces were stained for 30 min at 4°C with anti-mouse B220 (Biolegend, APC), GL7 (Biolegend ...
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bioRxiv - Cell Biology 2022Quote: ... and the cells were incubated for 15 minutes at 4°C in streptavidin-PE/Cy7 (BioLegend) to detect NCAM-biotin ...
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bioRxiv - Molecular Biology 2022Quote: ... samples were first incubated for 1h at 4°C with 2μl of anti- HA antibody (BioLegend) and then for another hr after adding 30μl of washed magnetic ProteinG beads (Cytiva) ...
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Two subsets of human marginal zone B cells resolved by global analysis of lymphoid tissues and bloodbioRxiv - Immunology 2021Quote: ... Cells were then incubated for 10 minutes at 4°C with True stain Fc block (BioLegend) and washed ...
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bioRxiv - Immunology 2019Quote: ... macrophage activation was induced by adding either IL-4 (20 ng/ml, Biolegend, San Diego, USA), IL-13 (20 ng/ml ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by overnight staining at 4°C with rat anti-mouse CD45 primary antibody (103102, Biolegend) diluted 1:500 in 1x D-PBS ...
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bioRxiv - Immunology 2020Quote: ... Th2 cells were cultured for 4 days with plate-bound αCD3 (5 μg/ml; Biolegend, #100339), rIL-4 (4 ng/ml ...
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bioRxiv - Immunology 2022Quote: ... Concentrations of IFN-γ and IL-4 in supernatants were analyzed by DuoSet ELISA kit (Biolegend) according to the manufacturer’s instructions.
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bioRxiv - Neuroscience 2022Quote: ... that had previously been linked with 4 μg each of mouse monoclonal 6E10 and 4G8 (Biolegend) were added to the neutralized FA fraction ...
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bioRxiv - Neuroscience 2023Quote: ... supernatant was removed by aspiration and 500 microliters of fixation buffer (4% paraformaldehyde in PBS, Biolegend) added to each well and the slide kept in the dark at room temperature ...
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bioRxiv - Immunology 2023Quote: ... Plates were washed 4 times with PBS and 40μl of HRP anti-rabbit IgG antibody (Biolegend) 1:1500 was added to each well and incubated for 2 hours at room temperature ...
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bioRxiv - Immunology 2023Quote: ... and stained (30 min at 4°C) with PE anti-TLR4 (Biolegend; clone Sa15-21; 145404) in blocking buffer (PBS with 2% rat serum) ...
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bioRxiv - Genetics 2021Quote: ... production from LPS- or OL-treated BMDM were performed using Mouse TNF-α ELISA MAX Deluxe kit and Mouse IL-6 ELISA MAX Deluxe kit (BioLegend, San Diego, CA, USA). Follow-up cytokine (IL1β ...
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bioRxiv - Cell Biology 2019Quote: ... cells were then incubated for 10 minutes at 4°C in human Fc Blocking reagent (FcX, BioLegend). Cells were incubated at 4°C for a further 30 minutes with 0.5µg of TotalSeq™-A (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... lymph node cell IL-1β/IL-23 stimulation group (LN stim) with hashtag 4 (BioLegend, Cat# 155807); lymph node cell IL-1β/IL-23 plus spermidine treatment group (LN sper ...
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bioRxiv - Genetics 2019Quote: ... anti-CD25-PE (8 µl) and anti-CD45RA-BV785 (4 µl, clone HI100, Cat. no. 304140, BioLegend), incubated at RT in the dark for at least 30 min. ...
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bioRxiv - Bioengineering 2021Quote: ... which were incubated overnight at 4 °C with primary antibodies diluted 1:5,000 in blocking buffer (BioLegend Way ...
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bioRxiv - Cell Biology 2021Quote: ... cells were then incubated for 10 minutes at 4°C in human Fc Blocking reagent (FcX, BioLegend). Cells were incubated at 4°C for a further 30 minutes with 0.5μg of TotalSeq-A (Biolegend ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were incubated at 4°C for a further 30 minutes with 0.5μg of TotalSeq-A (Biolegend) monoclonal anti-CD10 ...
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bioRxiv - Immunology 2021Quote: ... thymocytes were cultured for 4 hours in the presence of PMA and Ionomycin (Cell Stimulation Cocktail, Biolegend) and Brefeldin A (Biolegend) ...
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bioRxiv - Cancer Biology 2020Quote: ... we incubated the nanowell array with 4 µg/ml PE anti-CD63 antibody (BioLegend, clone NVG-2) for 45 minutes at 37°C ...
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Nanoparticle-delivered TLR4 and RIG-I agonists enhance immune response to SARS-CoV-2 subunit vaccinebioRxiv - Immunology 2022Quote: ... cell surfaces were stained for 30 min at 4°C with anti-mouse CD8a (Biolegend, PE/Cy5), CD4 (Biolegend ...
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bioRxiv - Microbiology 2022Quote: ... stained for 30 minutes at 4℃ with the following antibody: APC/Cyanine7 anti-mouse CD45 antibody (Biolegend), PE/Cyanine7 anti-mouse CD4 antibody (Biolegend) ...
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bioRxiv - Cancer Biology 2022Quote: ... intracranial and systemic lymphocyte samples were stained with anti-human CTLA-4 antibody (Biolegend, clone BNI3, BV421). Additionally ...
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bioRxiv - Immunology 2020Quote: The isolated cells were then stained for 30 min at 4°C with the following antibodies (Biolegend): APC-Fire 750-anti CD11b (Cat ...
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bioRxiv - Genomics 2021Quote: ... Cells sorted for CTLA-4 were treated with Cell Activation Cocktail without Brefeldin A (Biolegend, Cat #423302) for 4 hours prior to fixation ...
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bioRxiv - Cell Biology 2021Quote: ... were blocked with 5ul Human TruStain FcX™ Blocking Reagent for 10 minutes at 4°C (BioLegend). 1ug of each TotalSeq™-A antibody was added to the cell suspension ...
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bioRxiv - Microbiology 2020Quote: ... cells were stained for 30 min at 4°C with FITC-conjugated anti-human CD3 antibody (Biolegend), followed by fixation and permeabilization of cells with BD Cytofix/Cytoperm™ (BD Bioscience) ...
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bioRxiv - Immunology 2021Quote: ... CD8 BUV496 or BV650 (2:100 or 4:100; BD Biosciences Cat# 612942 or Biolegend Cat# 301042), and Live/Dead eFluor506 (5:1000 ...
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bioRxiv - Immunology 2022Quote: ... at 4°C) and stained with a fixable viability dye (Zombie NIR™ Fixable Viability Kit, Biolegend) in PBS for 20 min at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Intracellular IFN-γ and IL-4 were stained with anti-IFN-γ-PE/Cyanine7 (clone XMG1.2, BioLegend), and anti-IL-4-PE (clone 11B11 ...
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bioRxiv - Immunology 2022Quote: ... Samples were incubated overnight at 4°C with anti-CD15(HI90)-Bi209 (primary antibody purchased from Biolegend; conjugated with Fluidigm’s MaxPar labeling kit ...
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Podoplanin expression in fibroblasts determines lymph node architecture and adaptive immune functionbioRxiv - Immunology 2022Quote: ... Cells were permeabilised for 20 minutes at 4°C using the FOXP3 Fix/perm buffer set (Biolegend) containing purified rat IgG2b anti-mouse CD16/CD32 receptor antibody ...