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Citations for BioLegend :
4651 - 4700 of 5050 citations for Recombinant Mouse CD19 Antigen His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... Placental neutrophils contained in the resulting single cell suspension were enriched via positive selection using 0.2μg anti-mouse Ly6G PE (Biolegend, clone 1A8) per 1×106 cells followed by anti-PE microbeads (Biolegend) ...
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bioRxiv - Microbiology 2022Quote: ... cells were stained in PBS with 0.5% BSA with Fixable Viability Dye eFluor 780 (eBioscience) and a combination of the following antibodies: anti-mouse CD45 (PE/Cy5, 30-F11, BioLegend), CD45 (Alexa Fluor 700 ...
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bioRxiv - Pathology 2022Quote: ... The pellet was further washed once with FACS buffer (0.5% BSA/0.5 mM EDTA in PBS) and stained with APC-conjugated anti-mouse CD45 (1:300, BioLegend, CAT#103112), BV510-conjugated anti-mouse CD11b (1:300 ...
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bioRxiv - Immunology 2022Quote: Circulating CD20+ cells were depleted by intraperitoneal administration of 200 μg of rat-anti-mouse-CD20-depleting antibody (Biolegend, 152104) two days before the start of TAM administration ...
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bioRxiv - Neuroscience 2022Quote: ... according to manufactures protocol and blocked in 5% BSA/TBS for 1 h and incubated overnight at 4 °C with the following antibodies: mouse anti-CD9 (1:1000; Biolegend), mouse anti-CD63 (1:1000 ...
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bioRxiv - Physiology 2022Quote: ... CD11b+-SVF were incubated with were with 0.2 mg/mL of anti-mouse PE-F4/80 (BioLegend, #12-4801-82) with utilization of PE rat IgG2a K isotype Control (BioLegend#12-4321-80 ...
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bioRxiv - Immunology 2022Quote: Slices were placed on a microscopy slide and Fc receptors on cells were blocked to avoid nonspecific staining using 20 μl purified anti-mouse CD16/32 antibody (BioLegend) in 1X PBS with 2% FBS at a concentration of 20μg/ml for 30 min at 37 °C ...
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bioRxiv - Immunology 2022Quote: ... Intra-cellular labelling for detection of IL-10 was performed using the Intracellular Fixation & Permeabilization Buffer Set (eBioscience) and the PE mouse anti-human Anti-IL-10 mAb (Biolegend). Cells were kept at 4°C in 1.25% paraformaldehyde until analysis ...
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bioRxiv - Immunology 2022Quote: Cells were stained with the following surface marker antibodies: anti-mouse CD45 (BV510™, BioLegend, clone 30-F11, cat# 103138), anti-mouse CD11c (BV785™ ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Cytokines in frozen cell culture supernatant or frozen plasma were analyzed with a customized multiplex human or mouse cytokine Legendplex kit (BioLegend). Samples were measured at the MACS Quant Analyzer10 (Miltenyi Biotec ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and at passage 1 a depletion of CD45+ cells was performed using a Biotin anti-mouse CD45 Antibody (Biolegend, 103104) and Dynabeads™ Biotin Binder (ThermoFisher 11047) ...
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bioRxiv - Microbiology 2022Quote: ... using 1:1000 of polyclonal rabbit anti-human MCT1 IgG (Cell Signalling) and 1:1000 mouse anti-human CD147 IgG (Biolegend) followed by HRP-conjugated secondary IgG antibody of specific host type ...
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bioRxiv - Microbiology 2022Quote: ... Identity and purity of eluted proteins was assessed by coomassie staining on 10 % SDS PAGE followed by Western blotting with mouse anti-human basigin IgG (Biolegend). Fractions were pooled and concentrated using Amicon centrifugal filter units 100 kDa (Merck ...
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bioRxiv - Immunology 2022Quote: IL-2 ELISA was performed using an ELISA MAX™ Deluxe Set Mouse IL-2 as described in manufacturer’s protocol (BioLegend). The O.D ...
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bioRxiv - Synthetic Biology 2024Quote: ... mice were intravenously injected with 20 µg of anti-mouse CD31 antibodies coupled with Alexa Fluor 647 (clone 390, Biolegend) to label blood vessels and ear was allowed to stabilize for 30 min ...
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bioRxiv - Immunology 2024Quote: ... CD4 conv T cells were resuspended in complete RPMI 10% FBS and incubated for 1 hour at 37C with 1 ug/ml of anti-mouse CD3 (2c11 clone; Biolegend) or left untreated ...
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bioRxiv - Immunology 2024Quote: ... CD4+ T-cells were enriched from the spleen of OT-II mice using the MojoSort mouse CD4+ T-cell isolation kit (BioLegend) and labelled with CellTrace Violet (5 μM ...
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bioRxiv - Cell Biology 2024Quote: ... 2 mM EDTA in PBS) and incubated with Fc-blocking reagent (1:200, TruStain FcX, anti-mouse CD16/32, BioLegend). After washing ...
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bioRxiv - Microbiology 2023Quote: ... The BALF was processed to isolate the cells upon RBC lysis and stained with PE anti-mouse Ly6G clone 1A8 (Biolegend) and FITC anti-mouse F4/80 clone BM8 (Biolegend ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cells were counted and then incubated at 4 °C for 25 min with FITC anti-mouse CD45 (Biolegend, 103107, USA), Brilliant Violet 650 Ly6G (Biolegend ...
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bioRxiv - Cancer Biology 2024Quote: ... PL1 or KPL1 antibodies were incubated for 1 h followed by PE conjugated goat anti-mouse secondary antibody (cat. no. 405307, Biolegend) for another hour ...
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bioRxiv - Neuroscience 2024Quote: ... and heavy non-phosphorylated neurofilament (NF-H) enriched in large pyramidal neurons (Anti-NF-H mouse monoclonal antibody, clone SMI32, 1:2000, Biolegend). All sections were counterstained with hematoxylin for further visualization of cell organization used in cytoarchitectonic type identification explained below and in Supplementary Table 1.
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bioRxiv - Molecular Biology 2024Quote: ... The cell suspensions were then labeled with lineage related antibodies: PE/Dazzle™ 594 anti-mouse/human CD11b (Biolegend 101256), APC anti-mouse Ly-6G/Ly-6C (Gr-1 ...
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bioRxiv - Microbiology 2024Quote: ... parasites were washed twice with FACS buffer and stained with (1:100) goat anti-mouse IgG-APC (BioLegend, cat# Poly4053). After incubation ...
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bioRxiv - Microbiology 2024Quote: Levels of cytokines and soluble mediators present in serum of infected mice were quantified using LEGENDplex Mouse Inflammatory Panel kit (BioLegend) according to the manufacturer’s protocol ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were immunolabeled in blocking solution for 1 hour at RT with antibodies against the following proteins: ALIX at 5 μg/ml (mouse; no. 634502, Biolegend); IST1 at 0.4 μg/ml (rabbit ...
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bioRxiv - Physiology 2023Quote: ... the resulting single cell suspension was incubated at 37°C for 30 minutes with Integrin β1 antibody (APC anti-mouse/rat CD29; 102216; Biolegend) at 1:100 dilution ...
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bioRxiv - Microbiology 2024Quote: ... The amount of Ag-specific IgG was then quantitated by running a western blot using mouse IgG (BioLegend CAT#944803) of known mass as a standard ...
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bioRxiv - Immunology 2024Quote: ... cells were washed again and stained with anti-mouse antibodies to intracellular markers anti-TNF-α-Alexa Flour 700 (Biolegend, Alexa Fluor® 700 anti-mouse TNF-α Antibody ...
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bioRxiv - Immunology 2024Quote: ... and anti-dsDNA antibodies were detected with a 1/1000 dilution of alkaline phosphatase–conjugated goat anti-mouse IgG (BioLegend) for 1 hour at 37°C and developed with a phosphatase substrate for 30 min at 37°C.
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bioRxiv - Immunology 2024Quote: ... Mouse anti-human HLA-E (clone 4D12) primary antibody (MBL Lifescience) and then secondary antibody goat anti-mouse conjugated with APC (BioLegend) were applied with staining buffer (DPBS ...
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bioRxiv - Immunology 2024Quote: ... Naive CD8+ T cells were isolated by negative selection using the MojoSort Mouse CD8+ Naïve T cell Isolation Kit as per manufacturer’s instructions (BioLegend, >90% purity). Live cells were counted by trypan blue dye exclusion and resuspended at a concentration of 106 cells/mL in complete T cell media (RPMI-1640 (Cytiva ...
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bioRxiv - Immunology 2024Quote: ... FcγII/III receptors were blocked by prior 10 min incubation with Fc block (anti mouse CD16/32 antibody Clone 93, Biolegend) on ice.
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bioRxiv - Immunology 2024Quote: ... for 1 h at room temperature in the dark following pretreatment with Ultra-LEAF purified anti-mouse CD16/32 Ab (clone 93, BioLegend) for 30 min ...
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bioRxiv - Immunology 2024Quote: ... which were obtained from indicated sources: APC anti-mouse I-A/I-E (MHC II) (clone M5/114.15.2, BioLegend, 1:500), FITC anti-mouse I-A/I-E (MHC II ...
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bioRxiv - Immunology 2024Quote: ... Cytokine concentration in these samples were determined using CBA mouse Inflammation Kit (BDTM) or ELISA according to the manufacturers’ instructions (Biolegend).
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bioRxiv - Immunology 2024Quote: ... The following antibodies were used for staining: Alexa Fluor 488 anti-mouse CD3 (17A2) (1:100) (BioLegend, San Diego, CA), PE anti-mouse CD4 (GK1.5 ...
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bioRxiv - Bioengineering 2023Quote: ... The culture supernatant was collected and the Th2 cytokines were measured using a LEGENDplex™ mouse Th cytokine assay (Biolegend) according to the manufacturer’s protocol.
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bioRxiv - Biochemistry 2023Quote: ... Cells were stained for sgRNA expression with an APC conjugated anti-mouse CD90.1/Thy1.1 antibody (no. 202526, BioLegend; 1:400) and Human TruStain FcX Fc receptor blocking solution (no ...
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bioRxiv - Immunology 2023Quote: ... the cells were re-suspended in flow cytometry staining buffer (PBS containing 4% FBS and 2 mM EDTA) and blocked with TruStain FcX (mouse or human, Biolegend) for 5 min at 4°C ...
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bioRxiv - Immunology 2023Quote: ... Enriched cells were washed once in wash buffer and an Fc blocking step was performed using TruStain FcX (anti-mouse CD16/32) antibody (BioLegend) according to the manufacturer’s instruction.
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bioRxiv - Genomics 2023Quote: ... control samples (Mouse IgG2a) were sorted on the basis of live single cells that are CD69− (PE-Dazzle 594, Biolegend) and the stimulated samples (anti-CD3 plus either anti-CD28 ...
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bioRxiv - Neuroscience 2023Quote: ... were used to confirm results with McSA1: a mouse monoclonal antibody to Aβ residues 1-16 (1:1000; clone 6E10; Cat#803001, Biolegend) or a mouse monoclonal antibody to Aβ residues 17-24 (1:1000 ...
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bioRxiv - Immunology 2023Quote: ... The phenotype of differentiated HL-60 cells was assessed by flow cytometry using mouse antihuman CD35 PE conjugated antibody (BioLegend) and mouse anti-human CD71 APC conjugated antibody (BioLegend) ...
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bioRxiv - Molecular Biology 2023Quote: ... Conditioned medium was collected and IL-1β levels were assessed with ELISA MAXTM Deluxe Set Mouse IL-1β (Biolegend, 432604) following manufacturer’s instructions.
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bioRxiv - Pathology 2023Quote: ... followed by the surface monoclonal mouse anti-human conjugated antibodies: anti-CD15-PE (clone H198) and anti-CD11b-FITC (ICRF44) from Biolegend. Intracellular staining was performed after fixation and permeabilization with Fix/Perm kit (eBiosciences) ...
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bioRxiv - Microbiology 2023Quote: ... Cells were re-suspended in flow cytometry buffer and incubated with TruStain FeX™ PLUS anti-mouse CD16/32 (BioLegend) (0.25 μg per 106 cells in 100 μL ...
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bioRxiv - Immunology 2023Quote: Staining for flow cytometry was done using the following anti-mouse mAbs: CD3 (17A2) conjugated to PE/Dazzle 594 (BioLegend), CD4 (RM4-5 ...
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bioRxiv - Immunology 2023Quote: ... were isolated from single cell suspension of lymph nodes and spleen by negative selection using the MojoSort Mouse CD8 T cell isolation kit (BioLegend). Alternatively ...
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bioRxiv - Microbiology 2023Quote: ... Adjacent sections were deparaffinized in citrate and stained with monoclonal mouse anti-F4/80 antibody at a 1:400 dilution (BioLegend), or polyclonal goat anti-MPO antibody at a 1:500 dilution (R&D Systems) ...