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Citations for BioLegend :
4451 - 4500 of 5050 citations for Recombinant Mouse CD19 Antigen His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Cells were resuspended at 20 ×106 cells/mL in Cell Staining Buffer and non-specific staining was then blocked by addition of 2μg/mL anti-CD16/32 (mouse TrueStain FcX Biolegend), and 2% rat serum (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... mouse monoclonal anti-Aβ (anti-β-amyloid 17-24, clone 4G8, #800712, 1:500, Biolegend, San Diego, CA, USA) overnight at 4°C ...
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bioRxiv - Molecular Biology 2021Quote: ... Fc receptors were blocked by incubating cells with TruStain FcX™ PLUS anti-mouse CD16/32 antibody (Biolegend, USA) for 10 min ...
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bioRxiv - Microbiology 2021Quote: ... in PBS containing the primary antibodies at a 1:100 dilution: mouse anti-DC/L-SIGN (Biolegend, cat. 845002), rabbit anti-DC-SIGN (Cell Signaling ...
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bioRxiv - Neuroscience 2019Quote: ... in Alexa Fluor® 488) and rat anti-mouse CD31 (1:100; Biolegend 390 in APC/Fire™ 750) for 30 minutes at 4°C ...
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bioRxiv - Immunology 2020Quote: ... Following antibodies were used for flow cytometry (all 5µg/ml, except indicated differently): rat anti mouse CD45 (clone 30-F11, BioLegend), rat anti mouse CD11b (clone M1/70) ...
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bioRxiv - Bioengineering 2020Quote: ... and treated with Fc-receptor blocker (2.5 μ g/mL, anti-mouse CD16/32; BioLegend, Cat# 101301, RRID: AB_312800) for 20 minutes at 4 °C in the dark ...
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bioRxiv - Cancer Biology 2021Quote: Single cell suspensions isolated from treated tumors are individually barcoded with hashtag antibodies (Anti-mouse TotalSeq-C antibodies, Biolegend). Each sample was washed 4 times with PBS + 2% BSA before pooling ...
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bioRxiv - Immunology 2019Quote: 106 cells in 100 μL FACS buffer were stained with fluorochrome-labelled antibodies for macrophage type-specific cell surface markers: anti-mouse CD23 (cat. # 101618, clone B3B4, Per.CP/Cy5.5, 7 μl, Biolegend, CA), anti-mouse CD38 (cat ...
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bioRxiv - Immunology 2019Quote: ... dissociated GL261 tumors (5 × 106 cells) were pre-incubated with 1.0 μg of TruStain fcX (anti-mouse CD16/32, clone 93; BioLegend) in 100 μL of FACs Buffer (DPBS with 1% BSA ...
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bioRxiv - Immunology 2021Quote: T cells were enriched from single cell suspensions of splenocytes isolated from naïve C57BL/6 mice as for flow cytometry using a mouse CD3 T cell negative selection kit (Biolegend). Enriched CD3+ T cells were cell trace stained and cultured at 1x106 cells/mL and stimulated with mouse T-activator anti-CD3/CD28 Dynabeads (ThermoFisher ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were collected by scraping and stained with APC-conjugated anti-mouse H-2Kb bound to SIINFEKL (141606, BioLegend), PerCP-conjugated anti-mouse CD86 (105025 ...
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bioRxiv - Cell Biology 2021Quote: ... Then, cells were staining with CD8 (mouse CD8-APC-Cy7, eBioscience, 47-0081-82) and Zombie UV (Biolegend, 427107), followed by flow cytometry analysis.
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bioRxiv - Bioengineering 2021Quote: ... and the supernatant was used to measure secreted cytokines using a LEGENDplex™ Mouse Th Cytokine Panel kit (BioLegend) according to the manufacturer’s instruction ...
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bioRxiv - Cell Biology 2021Quote: ... Unfractionated or c-Kit-enriched BM cells were then incubated with purified rat anti-mouse lineage antibodies (CD3, BioLegend, 100202 ...
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bioRxiv - Immunology 2020Quote: ... Monoclonal mouse IgG1 anti-human CD64 FITC-conjugated (clone 10.1) and monoclonal mouse IgG2a anti-human CD59 PE-conjugated were from BioLegend, San Diego ...
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bioRxiv - Microbiology 2021Quote: ... Alexa Fluor 647-labelled monoclonal mouse anti-human CD62P (P-Selectin) antibody (Clone AK4, BioLegend, San Diego, CA, USA), Alexa Fluor 647-labelled goat anti-mouse IgG (GAMIG AF-647 ...
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bioRxiv - Immunology 2020Quote: CD4+ T cells were harvested from the lymph nodes and spleens of naive NR1 mice and enriched with a mouse naïve CD4 negative isolation kit (BioLegend) following the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... followed by 10-minute incubation on ice with blocking buffer containing 10% mouse serum with 0.25 μg/ml anti CD16/CD32 (BioLegend). Cells where incubated with indicated antibodies (Supplementary Table 5 ...
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bioRxiv - Immunology 2020Quote: ... stimulated library was stained for its respective marker with the following antibody: MHCII (APC anti-mouse I-A/I-E Antibody, Clone M5/114.15.2 Biolegend 107613), CD40 (APC anti-mouse CD40 Antibody ...
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bioRxiv - Immunology 2020Quote: ... Blood and lymphatic vessels were stained with anti-CD31 AF647 (10μg per mouse, retro-orbital 15 min prior to imaging, clone 390, Biolegend) and anti-LYVE-1 AF647 (10μg per mouse ...
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bioRxiv - Molecular Biology 2020Quote: ... The following primary antibodies were used at the indicated concentrations: mouse anti-HA (1:200, HA.11 901501 Biolegend), mouse anti-BRCA1 D-9 (1:40 ...
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bioRxiv - Cancer Biology 2020Quote: ... T cells were isolated from spleen using MojoSort™ Mouse CD3 T Cell Isolation Kit (Biolegend Cat no; 480024) as per the manufacturers protocol ...
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bioRxiv - Bioengineering 2021Quote: ... cells were blocked (Biolegend FACS buffer with 0.05% anti-mouse CD16/32 Biolegend, 101320 and 0.05% Tru-stain monocyte blocker Biolegend, 426103) for 10 min at RT ...
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bioRxiv - Immunology 2021Quote: Human and mouse IL-6 and TNF-α protein were measured by an enzyme-linked immunosorbent assay (ELISA) (BioLegend) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... MR1 surface expression was evaluated by flow cytometry with APC-labeled mouse anti-MR1 mAbs 26.5 (Biolegend, Cat#361108) and APC-labeled mouse IgG2a (clone MOPC-173 Biolegend ...
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Bacterial Polyphosphates Induce CXCL4 and Synergize with Complement Anaphylatoxin C5a in Lung InjurybioRxiv - Immunology 2022Quote: ... preincubated for 20 min with anti-mouse CD16/CD32 antibody (TruStain FcX, BioLegend, San Diego, CA, USA, 1:50) in FACS buffer followed by 20 min incubation with fluorescent dye-conjugated ...
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bioRxiv - Immunology 2022Quote: ... CD8+ OT-I splenic T cells were isolated from crushed spleens of naïve OT-I mice using the Mouse CD8 T cell Isolation Kit (BioLegend). Sorted tumor APC populations were pulsed with 0.01 ng/mL SIINFEKL peptide (Invivogen ...
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bioRxiv - Immunology 2022Quote: ... Naïve CD4+ T cells were isolated from the spleen by using the MojoSort Mouse Naïve CD4+ T Cell Isolation Kit (#480040, BioLegend). CD4+ T cells isolated from the OT-II spleen were cocultured with BMDCs ...
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bioRxiv - Cell Biology 2022Quote: ... 1µg/mL for FC) APC mouse IgG1, k isotype Control (400120, 1µg/mL for FC) were purchased from Biolegend.
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bioRxiv - Immunology 2022Quote: ... and nonspecific staining was then blocked by addition of anti-CD16/32 (2 μg/ml; mouse TrueStain FcX, BioLegend), 2% rat serum (Invitrogen) ...
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bioRxiv - Immunology 2022Quote: After thawing cells were washed and Fc receptors were blocked with TruStain FcX (mouse or human as appropriate; Biolegend) for 5 minutes at room temperature before staining with diluted antibodies (Supplemental Tables 2 ...
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bioRxiv - Neuroscience 2023Quote: ... cat no: 130-123-284) and allophycocyanin (APC) conjugated rat anti-mouse CD45 (1:100, Biolegend, cat no: 103111) in blocking solution for 30 minutes on ice in the dark ...
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bioRxiv - Immunology 2022Quote: ... All enriched samples were stained with the following panel of TotalSeq-A-labeled anti-mouse antibodies to detect protein expression in the scRNA-seq dataset: Ly6C (HK1.4, BioLegend), CD44 (IM7 ...
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bioRxiv - Biochemistry 2024Quote: ... and then in a T cell activation analysis cocktail including: anti-mouse CD3 clone 17A2 APC conjugate (BioLegend, 100235), rat anti-mouse CD4 AlexaFluor700 conjugate (BioLegend ...
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bioRxiv - Cancer Biology 2024Quote: ... The cells were stained for VEGF-A using antibodies against mouse VEGF-A (cat# 512809, clone 2G11-2A05, Biolegend) and AlexaFluor647-conjugated secondary antibodies ...
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bioRxiv - Immunology 2024Quote: ... These fixed and permeabilized cells were further stained with mouse anti-human IFN-γ antibody conjugated with PE (BioLegend) and Brilliant Violet 421 conjugated mouse anti-human TNF antibody (BioLegend) ...
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bioRxiv - Neuroscience 2024Quote: ... a subset of mice were intravenously injected with 0.1mL solution containing 0.4mg/kg FITC anti-mouse CD45.2 antibody (Clone 104, Biolegend, Cat #109806) and 1% Texas Red solution.
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bioRxiv - Immunology 2023Quote: ... Antibody staining was performed on ice for 1 hour with APC/Cy7 anti-mouse CD45 (clone 30-F11; BioLegend), rat monoclonal FITC anti-mouse Ly6B.2 (clone 7/4 ...
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bioRxiv - Immunology 2023Quote: Cells harvested from in vitro Th17 differentiated cells were stained with the following antibodies against mouse CD3 (17A2, Biolegend), CD4 (GK1.5 ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies were: Syn303 [a mouse monoclonal antibody that recognises residues 1-5 of human α-synuclein (7)] (BioLegend) at 1:4,000 ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed once with cold HMI-9 and resuspended in 200μl cold HMI-9 with rat anti-mouse IgM-FITC (1:500, Biolegend). After one wash with cold HMI-9 ...
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bioRxiv - Immunology 2023Quote: ... and CD4+ cells were magnetically enriched using mouse negative selection CD4+ T cell isolation kits (STEMCELL Technologies and Biolegend). Live Tregs were sorted as fixable viability dyeneg (Thermo Fisher Scientific and Tonbo Biosciences) ...
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bioRxiv - Cell Biology 2023Quote: ... tumor cell phenotyping and mouse blood phenotyping were evaluated with the following anti-human antibodies: CD155 (SKII.4, Biolegend), CD19 (HIB19 ...
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bioRxiv - Cancer Biology 2023Quote: ... expanded CD4+ T cells were cocultured at different ratios with CFSE/CTV labeled CD8+ T cells and stimulated with Mouse T-Activator CD3/CD28 Dynabeads at 2 cells per bead ratio and 0.5ng/mL IL-2 (BioLegend). Cell culture media consisted with RPMI 1640 ...
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bioRxiv - Neuroscience 2023Quote: ... The supernatants were used to measure cytokines and chemokines using LEGENDplex™ Mouse Inflammation Panel (13-plex) (BioLegend 740446). In the case of CSF cell transplant ...
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bioRxiv - Neuroscience 2023Quote: ... 17-24 Antibody (1:1000, Clone 4G8, N-terminal, a.a. 17-24, human & mouse specific, 800708 BioLegend, CA, USA); Amyloid Precursor Protein (1:1000 ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies used for immunofluorescence on these tissues include: mouse anti-myelin basic protein (MBP) (1:1000; #808401 BioLegend), rabbit anti-MBP (1:200 ...
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bioRxiv - Immunology 2023Quote: ... followed by 100ul of Streptavidin nanobeads for 1×10^8 cells in 1ml of MACS buffer for 15min on ice (MojoSort Mouse pan B cell isolation kit II, Cat.no. 480088, Biolegend). The cell suspension was diluted and put on a pre-rinsed LS Column (Cat ...
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bioRxiv - Immunology 2023Quote: ... They were cultured in Th1-polarising media consisting of R10 with 1 μg/mL anti-mouse CD28 (102101, BioLegend), 5 μg/mL anti-mouse IL-4 (504102 ...