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Citations for BioLegend :
4501 - 4550 of 4647 citations for Recombinant Mouse Cd40lg Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2021Quote: ... and then resuspended in 10% FCS in PBS (100μl/well) containing TruStain FcX™ (anti-mouse CD16/32) antibody (Biolegend; 1:200) and incubated on ice for 20mins ...
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bioRxiv - Immunology 2020Quote: ... Culture supernatants thereof were removed and analyzed for IL-2 production as a correlate for T-cell activation using an ELISA MAX mouse IL-2 set (BioLegend, SanDiego, USA).
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bioRxiv - Immunology 2022Quote: Ten 10-week-old male wild type C57BL/6J mice were intravenously injected with an APC/Cyanine 7-conjugated anti-mouse CD45.2 antibody (Clone 104, BioLegend 109824, RRID: AB_830789) five minutes prior to euthanasia by CO2 inhalation ...
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bioRxiv - Immunology 2020Quote: ... The cells were subsequently resuspended in 100 µl of FACS buffer with a secondary anti-mouse-APC antibody (BioLegend, catalogue number-405308). The cells were incubated at 4 °C for 30 minutes on ice and washed with PBS as before ...
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bioRxiv - Immunology 2020Quote: ... Cells were subsequently washed twice with PBSF and labeled with 50 μL of APC-conjugated monoclonal mouse anti-hemagglutinin tag (HA).11 antibody (BioLegend, Cat # 901524), PE-conjugated goat anti-human IgG polyclonal antibodies (Southern Biotech ...
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bioRxiv - Developmental Biology 2019Quote: ... Cells were then incubated overnight at 4°C with primary antibody diluted in blocking solution (rabbit anti-FLAG, Sigma, 1:250 or mouse anti-Tuj1, Biolegend 1:500). After washing in PBS and incubation with secondary antibody (Alexa 647 anti-goat IgG 1:500 ...
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bioRxiv - Immunology 2021Quote: ... Slides were incubated ON at 4°C with mouse anti-human CD66b antibody (BioLegend, 305102, 1:100 for both IHC and IF), anti-human CD8 (Invitrogen ...
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bioRxiv - Microbiology 2021Quote: ... Proteins were electrophoretically transferred to a PVDF membrane and incubated with both of the following primary antibodies: α-TcpA polyclonal rabbit and α-RpoA monoclonal mouse (Biolegend). Then ...
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bioRxiv - Immunology 2020Quote: ... For investigating the formation of iBALT B cell and T cells were stained in lungs using primary antibody cocktail containing rat anti-mouse CD45R/B220 (BioLegend, Cat#103202) and Hamster anti mouse CD3 (BD ...
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bioRxiv - Immunology 2020Quote: ... the individual antibody hit at 5 μg/ml and/or ACE2-mFc was incubated with 1-2 million 1D8 spike+ cells and detected by anti-human-Fc PE (invitrogen, 12-4998-82) and FITC Goat anti-mouse-IgG (Biolegend, cat#405305) or APC Goat anti-mouse-IgG (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... both FITC conjugated anti-FLAG (CST, cat#8146) and ACE2-mFc (Sinobiologics, cat#10108-H05H) and FITC-anti-mouse-IgG (Biolegend, cat#405305) were incubated with 1-2 million 1D8 cells following standard FACS protocol and analyzed by Beckman.
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bioRxiv - Immunology 2020Quote: ... a combination of the following anti-mouse antibodies was used to stain for surface markers (all from BioLegend, eBioscience, or BD Biosciences): anti-CD3ε (145-2C11) ...
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bioRxiv - Immunology 2020Quote: ... Cells were resuspended in 75µL of Fc blocking medium (5 µg/ml Purified anti-mouse CD16/32 Antibody, Biolegend in FACS buffer), and incubated for 20 minutes on ice ...
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bioRxiv - Immunology 2020Quote: ... CD4T cells were negatively isolated from the digested spleen and LNs of indicated donor mice with Mouse CD4T Cell Isolation Kit (STEMCELL Technologies 19852 or BioLegend 480033) according to the manufacturer’s protocol and labeled with 1.0 μM CFSE (Thermo Fisher ...
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bioRxiv - Neuroscience 2022Quote: ... Cell suspensions were treated with anti-mouse-CD16/32 to block Fc receptors (BioLegend, 5 μg/mL, 20 min at 4 °C) before washing and transferring to PBS and treating with Zombie NIR (BioLegend ...
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bioRxiv - Microbiology 2022Quote: ... This membrane was blocked with 5% milk and then incubated simultaneously with rabbit polyclonal α-MshA (1:1000, gift of J. Zhu) and mouse monoclonal α-RpoA (1:1000, Biolegend) (as a loading control ...
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bioRxiv - Immunology 2022Quote: ... The following reagents and anti-mouse antibodies were used for flow cytometry: Zombie Violet™ fixable viability kit (BioLegend, San Diego, CA), CD45 (clone 30-F11 ...
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bioRxiv - Physiology 2023Quote: Concentrations of tumour necrosis factor (TNF)-α from tissue culture supernatants post- stimulation were measured using ELISA MAX Standard Set Mouse TNF-α kit (Biolegend) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2024Quote: ... and then surface labelled for 30 min at 4°C with TotalSeq-C0182 anti-mouse CD3 (clone 17A2, dilution 1:50, Biolegend, cat # 100263) and TotalSeq-C0002 anti-mouse CD8α (clone 53-6.7 ...
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bioRxiv - Immunology 2024Quote: ... CD4+ T cells were isolated by negative selection with the aid of magnetic beads (MojoSort™ Mouse CD4+ T Cell Isolation Kit, BioLegend #480033) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... FITC (or APC)-conjugated anti-CD177 mAb (clone MEM-166, mouse IgG1) and respective isotype controls were from BioLegend (San Diego, CA). FITC-conjugated goat anti-human Ig(H+L ...
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bioRxiv - Immunology 2024Quote: ... Primary lymphocytes isolated from skin draining lymph nodes from adult C57BL/6 mice were cultured on plates precoated with anti-mouse α-CD3 (3 μg/ml, Biolegend, #100340) and anti-mouse α-CD28 (3 μg/ml ...
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bioRxiv - Cell Biology 2024Quote: ... Cells was washed with PBS and preincubated with 5 ml of TruStain FcX™ PLUS (anti-mouse CD16/32) Antibody (Fc Receptor Blocking Solution, BioLegend, 156603) for 10 minutes on ice to reduce nonspecific immunofluorescent staining ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were washed 3 times with Cell Staining Buffer and stained with either Alexa Fluor (AF) 488 mouse anti-human BAX antibody (0.5 μg/mL, Biolegend, Cat. No. 633603) or AF488 Mouse IgG1 κ isotype control (5 μg/mL ...
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bioRxiv - Bioengineering 2024Quote: ... Selection for transgene-positive cells was confirmed by flow cytometry for mouse Lyt2 expression as a reporter gene (Biolegend, San Diego, CA). 3T3 cells were grown in DMEM with 10% FBS and 1% P/S ...
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bioRxiv - Bioengineering 2024Quote: ... were first stained with Zombie Aqua™ fixable viability kit (1:500 dilution in PBS) and Fc-blocked with TruStain FcXTM (anti-mouse CD16/32) antibody (BioLegend, CA, U.S.A.) (1:20 dilution in PBS (1% BSA) ...
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bioRxiv - Immunology 2023Quote: HLA-B heavy chain and β2-microglobulin (β2m) were probed using HC10 (mouse hybridoma procured from University of Michigan Hybridoma Core) and purified anti-β2m (Biolegend; catalog # 316302) antibodies.
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bioRxiv - Immunology 2023Quote: ... cells were incubated at 4degC with the following fluorochrome conjugated primary anti-mouse antibodies for 45 minutes (all Biolegend, San Diego, CA): anti-CD45 PE-CF594 (clone 30-F11) ...
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bioRxiv - Microbiology 2023Quote: Cytokine levels in the serum and spleen of mice infected with SPN were measured using ELISA MAX™ Standard Set Mouse IL-6 (BioLegend 431301) and ELISA MAX™ Standard Set Mouse IL-1β (BioLegend 432601 ...
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bioRxiv - Cancer Biology 2023Quote: Mice were sedated with ketamine and xylazine and then were injected with 2 ug of APC anti-CD45 (2 ug per mouse diluted in 100 uL PBS, Biolegend 30-F11) retro-orbitally ...
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bioRxiv - Cancer Biology 2023Quote: ... CD8+ T cells were isolated from the spleens of C57BL/6 mice using the MojoSort™ Mouse CD8+ T Cell Isolation Kit (Biolegend, #480035), followed by 1X Red Blood Cell lysis buffer (eBioscience ...
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bioRxiv - Neuroscience 2023Quote: CD4+ cells were isolated from pooled splenocytes and lymph node cells with the aid of magnetic beads (MojoSort™ Mouse CD4+ T Cell Isolation Kit, BioLegend #480033) as per the manufacturer’s instructions ...
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bioRxiv - Bioengineering 2022Quote: ... 100 µl of 105/ml aAPC-BLMs containing all three ligands were stained with 5 µl of anti-mouse IgG PE (BioLegend, Cat#406607) and anti-human HLA-DR PE (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... and anti-CD45.2 APC antibodies together with TotalSeq-C anti-mouse hashtag antibodies (anti-CD45 clone 30-F11, anti-H-2 clone M1/42, Biolegend, #155869 (MH5), #155871 (MH6) ...
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bioRxiv - Immunology 2023Quote: ... IFN-α and IL-6 were detected by the Mouse Anti-Virus Response Panel (13-plex) (Cat# 740622 BioLegend, San Diego, CA) and analyzed on the NovoCyte Flow Cytometer (Agilent ...
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bioRxiv - Immunology 2023Quote: ... PE rat IgG2a-isotype control antibody and PE mouse IgG2b-d κ isotype control antibody were purchased from BioLegend (Sandiego, CA, USA) and PE mouse IgG1-κ Isotype control antibody from BD Biosciences ...
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bioRxiv - Immunology 2023Quote: Serum BAFF quantitation was performed on plasma samples from 12-14 week-old Sh2b3E372K mice using the LEGENDplex Mouse B cell Panel – S/P (1-plex) w/VbP kit (BioLegend; cat # 740983) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... Sections were stained using a three-colour protocol with 1:100 of each of the following antibodies in IF blocking buffer at 4 °C O/N: Alexa Fluor 488® anti-mouse GL-7 (GL-7; Biolegend, #144612), PE anti-mouse CD3 (145-2C11 ...
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bioRxiv - Cancer Biology 2023Quote: The following antibodies were used for flow cytometry: Brilliant Violet 510™ anti-mouse CD45 Antibody (BioLegend, catalog #103137, clone 30-F11), PE/Dazzle™ 594 anti-mouse CD3ε Antibody (BioLegend ...
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bioRxiv - Neuroscience 2023Quote: ... were rehydrated in distilled H2O then stained with Nissl or immunohistochemical labelling was performed as above with anti-mouse Ly6G primary antibody (BioLegend, London, UK) and goat anti-rabbit secondary antibody.
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bioRxiv - Immunology 2023Quote: ... for 10 min at RT then stained for the following cell surface markers for 30 min at 4°C: CD3 (T cell marker, mouse anti-CD3-BV510, BioLegend, 1:50), CD14 (monocyte marker ...
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bioRxiv - Immunology 2023Quote: Cytokine production was quantified from cell supernatants or serum using the following ELISA kits: ELISA MAX Deluxe Set Mouse IL-10 (BioLegend, Cat# 431414), ELISA MAX Deluxe Set Human IL-10 (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... PerCP/Cy5.5-conjugated anti-mouse Ly6G mAb (Cat. #.: 127615) and 7-AAD Viability Staining Solution (Cat. #.: 420404) were purchased from BioLegend (CA, USA).
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bioRxiv - Immunology 2023Quote: CD8+ T cells were isolated from in vitro treated CAR-T cells using Mojosort™ Mouse CD8 T Cell Isolation Kit (Biolegend, 480035). After washing cells with PBS twice ...
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bioRxiv - Neuroscience 2023Quote: ... and stained overnight with Alexa 488-conjugated Alexa Fluor® 488 anti-tubulin β3 and Alexa Fluor® 647 anti-mouse/human CD324 (E-cadherin) antibodies (#801203 and #147308, BioLegend). Each batch of anti-tubulin β3 antibody was titrated before use to minimize background staining ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were stained with the following antibody cocktail diluted in flow cytometry staining buffer: mouse anti-human CD3 BV750 (BioLegend Cat 344846), mouse anti-human CD4 AF700 (BioLegend Cat 300526) ...
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bioRxiv - Neuroscience 2024Quote: ... cell preparation and cell surface marker staining was carried out as previously described.20 The following antibodies were used for staining: mouse anti-CD11b (Biolegend, Cat# 101257), mouse anti-CD19 (Biolegend ...
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bioRxiv - Neuroscience 2020Quote: 250 μg protein from each human brain sample lysate was used for immunoprecipitation (IP) with a combination of Tau12 (Biolegend, cat. no. 806501), Tau 5 (Abcam ...
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bioRxiv - Neuroscience 2019Quote: ... Supernatants containing 70 µg of total protein were used to determine IL-34 concentration using an IL-34 ELISA kit (BioLegend, San Diego, CA).
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bioRxiv - Immunology 2024Quote: ... Surface proteins were then stained at 4°C for 30 minutes using the following markers: NIR Zombie dye APC-Cy7 (Biolegend, Cat No. 423105), CD20 PE (Biolegend ...