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Citations for BioLegend :
4201 - 4250 of 4647 citations for Recombinant Mouse Cd40lg Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... Mouse IgG and human IgG were detected with separate secondary antibodies (goat anti-human IgG Fc, goat anti-mouse IgG Fc, BioLegend) at a dilution of 1:4,000 in blocking buffer and PNPP as described above.
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bioRxiv - Cell Biology 2021Quote: ... Unfractionated or c-Kit-enriched BM cells were then incubated with purified rat anti-mouse lineage antibodies (CD3, BioLegend, 100202; CD4, eBioscience, 16-0041-82; CD5, BioLegend, 100602; CD8, BioLegend, 100702 ...
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bioRxiv - Cell Biology 2021Quote: ... Unfractionated or c-Kit-enriched BM cells were then incubated with purified rat anti-mouse lineage antibodies (CD3, BioLegend, 100202; CD4, eBioscience, 16-0041-82; CD5, BioLegend, 100602 ...
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bioRxiv - Immunology 2020Quote: The following anti-mouse antibodies were used for tissue immunofluorescence: B220-PE (RA3-6B2) and CD3 FITC anti-CD3 (145-2C11) from Biolegend and CD138-APC (281-2 ...
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bioRxiv - Microbiology 2020Quote: ... Cell-line ACE2 expression levels were quantified by staining with anti-ACE2 antibody (NOVUS) and Alexa-fluor 594-conjugated goat anti-mouse IgG (Biolegend) and pacific blue viability dye ...
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bioRxiv - Immunology 2021Quote: ... Resulting single cell suspensions of splenocytes or bone marrow cells were Fc blocked with Trustain FcX (anti-mouse CD16/32 clone 93-Biolegend) and stained with combinations of the following antibodies ...
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bioRxiv - Immunology 2021Quote: ... Cells were stained with fluorescently-labeled antibodies directed against cell-specific surface marker for 30 min one ice in the dark (anti-mouse CD45-APC/Cy7, #103116, BioLegend; anti-mouse CD45-FITC ...
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bioRxiv - Immunology 2021Quote: ... The plates were washed twice in FACS buffer and stained with 50 μl/well of 1:200 dilution of PE-conjugated mouse anti-human IgG Fc antibody (BioLegend) on ice in dark for 1h ...
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bioRxiv - Immunology 2021Quote: ... fluorochrome-conjugated antibodies (anti-mouse CD45-BioLegend Cat. No. 103126, anti-human CD45-BioLegend Cat. No. 304014, anti-human CD3-BioLegend Cat ...
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bioRxiv - Immunology 2021Quote: ... Induced GC B-cells were then replated onto a fresh CD40LB feeder line and cultured with mouse IL-21 (10 ng/mL; BioLegend) or mouse IL-4 for another 3 to 4 days.
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bioRxiv - Immunology 2021Quote: ... splenocytes from immunized mice were stained with 1 μg/mL of goat F(ab’)2 anti-mouse Igκ-biotin followed by 1 μg/mL of streptavidin (BioLegend) at 4°C ...
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bioRxiv - Immunology 2021Quote: ... were coated overnight at 4°C with 50 µL/well of either 10 µg/mL (flat-bottom) or 5 µg/mL (round-bottom) anti-mouse CD3ε (BioLegend) in PBS (for stimulated wells) ...
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bioRxiv - Immunology 2021Quote: ... and finally with a mixture of AlexaFluor 647-conjugated rat anti-mouse Ly6C antibody (BioLegend, clone HK1.4; diluted 1:500) and DAPI (Molecular Probes ...
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bioRxiv - Immunology 2021Quote: ... CD8+ T cells were then isolated from the spleens using the MojoSort™ Mouse CD8 T Cell Isolation Kit (BioLegend), following the manufacturer’s guidelines ...
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bioRxiv - Cancer Biology 2022Quote: ... and 1×PBS) mixed with TruStain FcX™ (anti-mouse CD16/32) Antibody (1:200, Clone: 93, BioLegend, CA, USA) and eBioscience™ Fixable Viability Dye eFluor™ 780 (1:1000 ...
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bioRxiv - Cancer Biology 2022Quote: ... Alexa Fluor 647 anti-human SSEA-4 Antibody (#330408, clone MC-813-70, Mouse IgG3 κ, dilution 1:100, Biolegend); Cetuximab (#A2000 ...
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bioRxiv - Cancer Biology 2022Quote: ... For detection, anti-human IgG (clone HP6017, APC) or anti-mouse IgG2a (clone RMG2a-62, APC or BV421) both from BioLegend, were incubated for 20 min on ice ...
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bioRxiv - Genomics 2022Quote: ... Thermo Cat# 00-5523-00) followed by intra-cellular stains with anti-mouse IFNψ-PerCP/Cyanine5.5 (1:200, clone XMG1.2, BioLegend Cat#505821), T-bet monoclonal antibody-PE-Cyanine 7 (1:200 ...
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bioRxiv - Immunology 2022Quote: ... single cell suspensions of mouse lungs were washed with PBS prior to staining with Zombie NIR Fixable live/dead dye (Biolegend) for 20 min at room temperature (RT) ...
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bioRxiv - Immunology 2022Quote: ... The 1G4 murine CD8+ T cells were additionally stained with: mouse-anti-human TCR Vβ 13.1-PE (clone: H131, Biolegend, 362409). For in vivo studies ...
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bioRxiv - Neuroscience 2022Quote: ... Retinas were then placed in primary antibody solution containing mouse anti-SMI-32 (1:1000, BioLegend, Cat#801701, RRID: AB_509997) in blocking solution for 2-4 days at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... the BrdU-labeled splenocytes were surface stained for T-cell (CD3e-APC; eBioscience) marker after blocking with anti-mouse CD16/32 antibodies (BioLegend). Cells were then permeabilized and treated with DNase to expose BrdU epitopes followed by anti-BrdU-FITC and 7-AAD (7-amino-actinomycin D ...
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bioRxiv - Immunology 2022Quote: ... The membrane was washed three times with TBS-T for 10 min and subjected to the HRP-conjugated secondary goat anti-mouse antibody (Biolegend) in a solution of 3% non-fat milk in TBS-T for 1 h ...
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bioRxiv - Immunology 2022Quote: ... Placental neutrophils contained in the resulting single cell suspension were enriched via positive selection using 0.2μg anti-mouse Ly6G PE (Biolegend, clone 1A8) per 1×106 cells followed by anti-PE microbeads (Biolegend) ...
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bioRxiv - Immunology 2022Quote: Splenocytes isolated from four female mice (two Ezh2fl/fl and two CD19-Cre Ezh2fl/fl) were stained with BV421 anti-mouse CD19 antibody (Clone 6D5, BioLegend) and live/dead dye ...
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bioRxiv - Microbiology 2022Quote: ... cells were stained in PBS with 0.5% BSA with Fixable Viability Dye eFluor 780 (eBioscience) and a combination of the following antibodies: anti-mouse CD45 (PE/Cy5, 30-F11, BioLegend), CD45 (Alexa Fluor 700 ...
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bioRxiv - Immunology 2022Quote: The following antibodies were used to detect B cell populations in the BM: brilliant violet (BV) 421 anti-mouse CD19 antibody (Clone 6D5, BioLegend), PE/Cyanine5 anti-mouse/human CD45R/B220 (Clone RA3-6B2 ...
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bioRxiv - Pathology 2022Quote: ... The pellet was further washed once with FACS buffer (0.5% BSA/0.5 mM EDTA in PBS) and stained with APC-conjugated anti-mouse CD45 (1:300, BioLegend, CAT#103112), BV510-conjugated anti-mouse CD11b (1:300 ...
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bioRxiv - Immunology 2022Quote: Circulating CD20+ cells were depleted by intraperitoneal administration of 200 μg of rat-anti-mouse-CD20-depleting antibody (Biolegend, 152104) two days before the start of TAM administration ...
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bioRxiv - Neuroscience 2022Quote: ... according to manufactures protocol and blocked in 5% BSA/TBS for 1 h and incubated overnight at 4 °C with the following antibodies: mouse anti-CD9 (1:1000; Biolegend), mouse anti-CD63 (1:1000 ...
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bioRxiv - Physiology 2022Quote: ... CD11b+-SVF were incubated with were with 0.2 mg/mL of anti-mouse PE-F4/80 (BioLegend, #12-4801-82) with utilization of PE rat IgG2a K isotype Control (BioLegend#12-4321-80 ...
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bioRxiv - Immunology 2022Quote: Slices were placed on a microscopy slide and Fc receptors on cells were blocked to avoid nonspecific staining using 20 μl purified anti-mouse CD16/32 antibody (BioLegend) in 1X PBS with 2% FBS at a concentration of 20μg/ml for 30 min at 37 °C ...
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bioRxiv - Immunology 2022Quote: ... Intra-cellular labelling for detection of IL-10 was performed using the Intracellular Fixation & Permeabilization Buffer Set (eBioscience) and the PE mouse anti-human Anti-IL-10 mAb (Biolegend). Cells were kept at 4°C in 1.25% paraformaldehyde until analysis ...
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bioRxiv - Immunology 2022Quote: Cells were stained with the following surface marker antibodies: anti-mouse CD45 (BV510™, BioLegend, clone 30-F11, cat# 103138), anti-mouse CD11c (BV785™ ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Cytokines in frozen cell culture supernatant or frozen plasma were analyzed with a customized multiplex human or mouse cytokine Legendplex kit (BioLegend). Samples were measured at the MACS Quant Analyzer10 (Miltenyi Biotec ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and at passage 1 a depletion of CD45+ cells was performed using a Biotin anti-mouse CD45 Antibody (Biolegend, 103104) and Dynabeads™ Biotin Binder (ThermoFisher 11047) ...
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bioRxiv - Microbiology 2022Quote: ... using 1:1000 of polyclonal rabbit anti-human MCT1 IgG (Cell Signalling) and 1:1000 mouse anti-human CD147 IgG (Biolegend) followed by HRP-conjugated secondary IgG antibody of specific host type ...
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bioRxiv - Microbiology 2022Quote: ... Identity and purity of eluted proteins was assessed by coomassie staining on 10 % SDS PAGE followed by Western blotting with mouse anti-human basigin IgG (Biolegend). Fractions were pooled and concentrated using Amicon centrifugal filter units 100 kDa (Merck ...
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bioRxiv - Immunology 2022Quote: IL-2 ELISA was performed using an ELISA MAX™ Deluxe Set Mouse IL-2 as described in manufacturer’s protocol (BioLegend). The O.D ...
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bioRxiv - Synthetic Biology 2024Quote: ... mice were intravenously injected with 20 µg of anti-mouse CD31 antibodies coupled with Alexa Fluor 647 (clone 390, Biolegend) to label blood vessels and ear was allowed to stabilize for 30 min ...
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bioRxiv - Immunology 2024Quote: ... CD4 conv T cells were resuspended in complete RPMI 10% FBS and incubated for 1 hour at 37C with 1 ug/ml of anti-mouse CD3 (2c11 clone; Biolegend) or left untreated ...
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bioRxiv - Immunology 2024Quote: ... CD4+ T-cells were enriched from the spleen of OT-II mice using the MojoSort mouse CD4+ T-cell isolation kit (BioLegend) and labelled with CellTrace Violet (5 μM ...
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bioRxiv - Cell Biology 2024Quote: ... 2 mM EDTA in PBS) and incubated with Fc-blocking reagent (1:200, TruStain FcX, anti-mouse CD16/32, BioLegend). After washing ...
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bioRxiv - Microbiology 2023Quote: ... The BALF was processed to isolate the cells upon RBC lysis and stained with PE anti-mouse Ly6G clone 1A8 (Biolegend) and FITC anti-mouse F4/80 clone BM8 (Biolegend ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cells were counted and then incubated at 4 °C for 25 min with FITC anti-mouse CD45 (Biolegend, 103107, USA), Brilliant Violet 650 Ly6G (Biolegend ...
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bioRxiv - Cancer Biology 2024Quote: ... PL1 or KPL1 antibodies were incubated for 1 h followed by PE conjugated goat anti-mouse secondary antibody (cat. no. 405307, Biolegend) for another hour ...
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bioRxiv - Neuroscience 2024Quote: ... and heavy non-phosphorylated neurofilament (NF-H) enriched in large pyramidal neurons (Anti-NF-H mouse monoclonal antibody, clone SMI32, 1:2000, Biolegend). All sections were counterstained with hematoxylin for further visualization of cell organization used in cytoarchitectonic type identification explained below and in Supplementary Table 1.
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bioRxiv - Molecular Biology 2024Quote: ... The cell suspensions were then labeled with lineage related antibodies: PE/Dazzle™ 594 anti-mouse/human CD11b (Biolegend 101256), APC anti-mouse Ly-6G/Ly-6C (Gr-1 ...
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bioRxiv - Microbiology 2024Quote: ... parasites were washed twice with FACS buffer and stained with (1:100) goat anti-mouse IgG-APC (BioLegend, cat# Poly4053). After incubation ...
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bioRxiv - Immunology 2024Quote: ... Following volumes were added in a single tube containing 100ul FACS buffer per sample: 0.625uL per sample of APC/Cyanine7 anti-mouse CD19 (Biolegend 152412), 1.25uL per sample of Brilliant Violet 421™ anti-mouse CD38 (Biolegend 102732) ...