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Citations for BioLegend :
4451 - 4500 of 4522 citations for IFN gamma Mouse HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2019Quote: ... mouse anti-human CD247 (CD3ζ) mAb (clone 6B10.2) and mouse anti-human EGFR antibody (clone AY13) were purchased from Biolegend (San Diego, CA, USA). Anti-human CD32 mAb (clone 8.26 ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were washed with 2 ml of FACS buffer followed by 300 μl of FACS buffer and resuspended in a total of 100 μl of Brilliant Violet 650 mouse anti-human CD45 (Biolegend catalog no. 304043), Alexa Fluor 700 mouse anti-human CD4 (Biolegend ...
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bioRxiv - Neuroscience 2020Quote: ... anti-neurofilament to a phosphorylated heavy chain neurofilament (NF-H; mouse anti-human monoclonal antibody SMI-34, dilution 1:100, epitope retrieval time 10 min, Biolegend 835503; Biolegend, San Diego, CA). Hemosiderin deposition was evaluated on a section with only counterstain ...
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bioRxiv - Biochemistry 2021Quote: ... For detection in immunoassays monoclonal mouse anti-CD81 (clone M38), anti-CD9 (Clone VJ1/20) (Immunostep, S.L., Salamanca Spain) and IgG1 (MOPC-21, Biolegend, San Diego, California, USA), all directly conjugated to PE were used at 0.02 μg/μl.
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bioRxiv - Molecular Biology 2021Quote: ... 11 Satb1 cKO) of varying age 1-7 months by the LEGENDplex (13-plex) Mouse Th Cytokine Panel V02 (Biolegend, 740741; Lot B289245) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: Mice were bled from the submandibular vein and the erythrocytes in the blood were lysed with ACK lysis buffer and stained with the following antibodies: mouse-CD45-PECy7 (BioLegend; clone 30-F11), human-CD45-Pacific Orange (Thermo Fisher ...
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bioRxiv - Immunology 2021Quote: BMDMs were generated by culturing total BM cells for 7 days in complete RPMI supplemented with 10 ng/mL recombinant mouse M-CSF (BioLegend, Cat# 576406M-CSF). C8-S cells (ATCC ...
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bioRxiv - Cancer Biology 2022Quote: ... stained for 20 minutes on ice with the following antibodies at the following concentrations: anti-mouse CD49b 1:500 (#103511 Biolegend, San Diego, CA), anti-human CD49b 1:750 (#359310 Biolegend) ...
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bioRxiv - Immunology 2022Quote: Activated γδT cells were seeded in round-bottom plates with FITC conjugated anti-mouse CD107a (1D4B) antibody or anti-human CD107a (H4A3) (Biolegend, San Diego, CA). γδT cells were co-cultured with A20 or K562 at a ratio of 1:1 ...
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bioRxiv - Neuroscience 2022Quote: ... human Aβ was immunodepleted from TgCM with the mouse antibody 6E10 (Purified anti-β-Amyloid, 1-16 antibody BioLegend Cat# 803004, RRID: AB_2715854) and Protein G Sepharose beads (Sigma-Aldrich) ...
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bioRxiv - Immunology 2022Quote: ... Samples were washed with 15 mL of wash buffer (RPMI with 1% FBS) and the CD4+ T cells were enriched using MojoSort™ Mouse CD4 cell isolation (Biolegend, Cat#480006). The cells were stained and sorted as explained before in scRNAseq section as well ...
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bioRxiv - Bioengineering 2024Quote: ... Sections were then stained overnight at 4 °C in a humidified chamber with mouse monoclonal anti-human CD68 (hCD68, 1:400, BioLegend, clone Y1/82A) and rabbit polyclonal anti-mouse IL-10 (mIL-10 ...
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bioRxiv - Cancer Biology 2024Quote: ... Spleenocytes were resuspended in 3 ml MojoSort™ buffer and enriched for CD8+ T cells using the MojoSort™ Mouse CD8 T Cell Isolation Kit (BioLegend, 480035), following manufactureŕs instructions.
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bioRxiv - Cancer Biology 2024Quote: ... Filtered single cell suspensions were blocked (anti-mouse CD16 / CD32) and stained for live/dead discrimination (Zombie Aqua, BioLegend, or EMA, Thermo Fisher) prior to staining with fluorophore-conjugated antibodies (30 min ...
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bioRxiv - Molecular Biology 2023Quote: ... and spleen were counted on a Pentra ES60 Haematology Analyser (Horiba) and stained with anti-mouse antibodies against the following antigens as appropriate (all from BioLegend unless otherwise stated) in FACS buffer (PBS with 2% BSA and 0.025% sodium azide) ...
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bioRxiv - Physiology 2024Quote: IL-1β concentrations in the conditioned medium from murine splenic cells were measured by ELISA kits (mouse IL-1β, 432604, BioLegend, San Diego, USA) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: NK cells were enriched from splenocytes from WT or Pvrig KO C57BL/6 mice by using the MojoSort™ Mouse NK Cell Isolation Kit (Biolegend, cat. 480049). Enriched NK cells were cultured and stimulated in vitro in RPMI-1640 Medium (ATCC ...
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bioRxiv - Immunology 2024Quote: ... and incubated at 37°C for 30 min with either a 1/500 dilution of PE-labeled anti-6xHis tag mouse MAb (Biolegend, San Diego, CA) to detect VHHs or Alexa Fluor 647-labeled goat anti-human IgG (H+L ...
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bioRxiv - Developmental Biology 2023Quote: ... 10 mg of protein G agarose beads were crosslinked to 5 μL of mouse anti-HA antibody (BioLegend, 901501, 1:1000, RRID: AB_2565006) using an effective concentration of 6.5 mg mL-1 DMP ...
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bioRxiv - Neuroscience 2023Quote: ... Extracellular deposits of Aβ peptide were immunostained with the mouse anti-β-amyloid 17-24 antibody (1:5000, clone 4G8, BioLegend, Koblenz, Germany). Alpha-synuclein pathology was detected with the anti-syn-1 antibody (1:2000 ...
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bioRxiv - Microbiology 2023Quote: ... Samples were stained immediately after fixation with Alexa Fluor 647-labeled mouse-anti-human CD56 (10 μg/mL, Biolegend, San Diego, CA, USA) for 1 h and washed with PBS (Gibco ...
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bioRxiv - Microbiology 2023Quote: ... was added to measure and gate on the % live HL-60 cells and anti-mouse/human CD11b PE (Biolegend, Catalog #101207, 1:50) was added to measure and gate on differentiated HL-60 cells ...
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bioRxiv - Immunology 2023Quote: ... peripheral blood or BM were incubated for 20 min at 4°C with the following anti-mouse conjugated antibodies: anti-CD45-PerCP/Cy5.5 (BioLegend, clone 30-F11, #103132), anti-CD45.1-PE/Cyanine7 (BD Biosciences ...
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bioRxiv - Cell Biology 2023Quote: ... The following primary antibodies were used to validate siRNA knock-down in fibroblasts: anti-α2-spectrin (mouse IgG2b clone D8B7, Biolegend #803201, 1:1000), anti-β2-spectrin (BD Biosciences #612563 ...
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bioRxiv - Microbiology 2023Quote: ... A combination of selected antibodies (anti-mouse) was used depending on the purpose of each study: CD45 APC-Cy7 (Biolegend, clone 30-F11), CD8a FITC (Tonbo ebioscience ...
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bioRxiv - Immunology 2023Quote: ... On day of being assayed aliquots were thawed slowly on ice before being tested using the flow cytometry based LEGENDplex™ Mouse Inflammation Panel (BioLegend Cat: 740446) according to the manufacturer’s instructions for sample processing ...
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bioRxiv - Cancer Biology 2023Quote: ... NKG2D and DNAM-1 surface receptors on NK cells were blocked utilizing 25 μg/ml of purified anti-mouse NKG2D (Biolegend, clone CX5, 130202), purified anti-mouse DNAM-1 (Biolegend ...
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bioRxiv - Bioengineering 2024Quote: ... Sections were then stained overnight at 4 °C in a humidified chamber with rat monoclonal anti-mouse CD68 (1:400, BioLegend, clone FA-11) and rabbit polyclonal anti-mouse IL-10 (1:25 ...
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bioRxiv - Physiology 2023Quote: CD8+ cells were isolated from the spleens of four 8-week-old diabetic female NY8.3 NOD mice using a MojoSort Mouse CD8 T-cell isolation kit (Cat# 480043, BioLegend, San Diego, CA, USA) and MojoSort buffer (Cat# 480017 ...
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bioRxiv - Immunology 2024Quote: ... Cell pellets were resuspended in 100 µL of Fc-blocking medium (5 µg/ml Purified anti-mouse CD16/32 Antibody (Biolegend, San Diego, CA) in FACS buffer) ...
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bioRxiv - Cell Biology 2024Quote: ... non-specific Fc receptor binding was blocked using 1:100 dilution of TruStain FcX anti-mouse CD16/32 antibody (BioLegend, RRID:AB_1574975, Cat#101320). 5 min after Fc blocker treatment ...
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bioRxiv - Immunology 2024Quote: ... Cells were resuspended by mild vortexing and 50 ul of 2.5 µg/ml final (meaning 1/200 dilution of the stock) Fc-block (anti-mouse anti-CD16/32 antibodies, TruStain FcX from Biolegend, San Diego, CA) were added with the following addition of 1/200 dilution of antibodies for surface marker staining and Aqua Live/Dead dye (Thermo Fisher Scientific ...
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bioRxiv - Cell Biology 2020Quote: ... for 10 minutes at 4°C followed by staining with antibody cocktail containing Pacific-Blue-conjugated anti-mouse CD45 (clone 30-F11; Biolegend, San Diego, CA, USA), PE-conjugated anti-mouse Ly-6G (Gr-1 ...
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bioRxiv - Microbiology 2021Quote: ... transferred to a nitro-cellulose membrane and probed with polyclonal rabbit anti CupA (1:1000) and monoclonal mouse anti-RNA polymerase (1:3000, Biolegend, San 397 Diego, CA). Western blots were then probed with IRDye-conjugated anti-mouse and anti-rabbit secondary antibodies (both at 1:15,000 ...
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bioRxiv - Cancer Biology 2022Quote: ... was analyzed and monitored longitudinally by weekly bleeding to quantify hCD45+ cells in the peripheral blood by flow cytometry with human CD45-PE and anti-mouse CD45-APC-Cy7 antibodies (Biolegend, San Diego, CA, USA). Mice were monitored closely to detect disease onset and treatment started when hCD45+ cells were detectable in the peripheral blood ...
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bioRxiv - Immunology 2019Quote: Mice were intranasally dosed with 3 µg in 20 µl per mouse recombinant murine TNF-α (r-TNF-α, carrier-free, 575204, BioLegend Ltd, UK) every day for 3 days ...
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bioRxiv - Immunology 2020Quote: ... and incubated with FC block and subsequently stained with a combination of the following anti-mouse antibodies (all from BioLegend, eBioscience, or BD Biosciences): anti-CD8α (53-6.7) ...
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bioRxiv - Immunology 2021Quote: ... Naïve CD8+ T cells were then negatively-selected (CD8+CD44- purity > 90%) from the resulting single-cell splenocyte suspension using the MojoSort™ Mouse CD8 Naïve T Cell Isolation Kit (BioLegend, San Diego, CA, USA). Two modifications were introduced relative to the manufacturer’s guidelines to increase purity ...
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bioRxiv - Immunology 2021Quote: ... The cells were stimulated with 20μg/ml of a purified F(ab’)2 goat anti-mouse IgM (μ chain) antibody (BioLegend, Clone Poly21571, Cat# 157102) at 37°C for 10 seconds ...
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bioRxiv - Cancer Biology 2024Quote: ... APC-labeled EGFR monoclonal antibody (clone AY13, cat: #352906), Ki67 (cat: # 350514), and isotype control (mouse IgG1, κ, clone MOPC-21, cat: #400120) were purchased from BioLegend (San Diego, CA). EGFR monoclonal antibodies (cetuximab and nimotuzumab ...
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bioRxiv - Cancer Biology 2023Quote: ... peripheral blood and spleens were harvested from each mouse and the infiltrated primary cells were identified using flow cytometry by staining for human CD45 antibody (BioLegend; BLG-368522; 1:200).
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Pathogenic CD8 T cell responses are driven by neutrophil-mediated hypoxia in cutaneous leishmaniasisbioRxiv - Immunology 2023Quote: ... The slides were washed with PBS and incubated for 4 hours at room temperature with rat anti- mouse Ly6G (cone 1A8, BioLegend, San Diego, CA, USA). Slides were washed with PBS and incubated separately with Alexa Fluor 568 goat anti-rabbit (Invitrogen ...
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bioRxiv - Immunology 2023Quote: ... cells were washed with 3ml flow buffer (PBS with 2% FBS) at 300xg and stained with goat anti-mouse IgG-APC (BioLegend, clone Poly4053, catalog # 405308) and rat anti-mouse IgM-PE (ThermoFisher ...
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The loss-of-function of SOCS2 increases the inflammatory response to Staphylococcus aureus infectionbioRxiv - Immunology 2023Quote: ... Individual cytokine detection kits were also used to quantify mouse IL-6 and TNF-α (Bio-techne, France) and GM-CSF (Biolegend, San Diego, USA).
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bioRxiv - Bioengineering 2023Quote: ... the slices were transferred into 30% sucrose in water for >24 hours and then sub-sectioned on a sliding microtome to 15-30 μm for immunostaining using the following antibodies: mouse anti-HA (Biolegend catalog #901513, 1:1000) and rabbit anti-NeuN (Millipore catalog #ABN78 ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: Isolated aortic single cells were stained with Fc receptor blocker (TruStain FcX anti-mouse CD16/32; 101319; BioLegend, San Diego, CA, USA, 1:100) for 10 min at room temperature ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were stained with unconjugated rat anti-mouse lineage-specific antibodies (Ter-119, Mac1, Gr-1, B220, CD5, CD3, CD4, CD8, and CD127, Biolegend, San Diego, CA, USA), followed by incubating with Dynabeads anti-rat IgG (Thermo Fisher) ...
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bioRxiv - Microbiology 2023Quote: ... the FOXP3/Transcription Factor Staining Buffer kit (eBioscences – 00-5523-00) was used along with the following anti-mouse antibodies: BV605-conjugated anti-CD4 (Biolegend, RM4-5; 1/700), BV650-conjugated anti-B220 (BD Biosciences ...
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bioRxiv - Cell Biology 2024Quote: ... A C-terminal fragment of Sch9–5HA was detected using a mouse monoclonal anti-HA antibody (AB2565007, cat. # 901502; BioLegend, San Diego, CA, USA). Atg13 was detected using an anti-Atg13 antibody gifted by Dr ...
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bioRxiv - Neuroscience 2024Quote: ... flow cytometry staining master mix was prepared containing 1:200 dilutions of PE anti-mouse/human CD11b Antibody (BioLegend, Cat#:101208, clone M1/70), APC anti-mouse CX3CR1 (Antibody BioLegend ...