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Citations for BioLegend :
4101 - 4150 of 4522 citations for IFN gamma Mouse HEK293 since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2022Quote: ... Thermo Cat# 00-5523-00) followed by intra-cellular stains with anti-mouse IFNψ-PerCP/Cyanine5.5 (1:200, clone XMG1.2, BioLegend Cat#505821), T-bet monoclonal antibody-PE-Cyanine 7 (1:200 ...
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bioRxiv - Immunology 2022Quote: ... single cell suspensions of mouse lungs were washed with PBS prior to staining with Zombie NIR Fixable live/dead dye (Biolegend) for 20 min at room temperature (RT) ...
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bioRxiv - Immunology 2022Quote: ... The 1G4 murine CD8+ T cells were additionally stained with: mouse-anti-human TCR Vβ 13.1-PE (clone: H131, Biolegend, 362409). For in vivo studies ...
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bioRxiv - Neuroscience 2022Quote: ... Retinas were then placed in primary antibody solution containing mouse anti-SMI-32 (1:1000, BioLegend, Cat#801701, RRID: AB_509997) in blocking solution for 2-4 days at 4°C ...
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bioRxiv - Microbiology 2022Quote: ... the BrdU-labeled splenocytes were surface stained for T-cell (CD3e-APC; eBioscience) marker after blocking with anti-mouse CD16/32 antibodies (BioLegend). Cells were then permeabilized and treated with DNase to expose BrdU epitopes followed by anti-BrdU-FITC and 7-AAD (7-amino-actinomycin D ...
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bioRxiv - Immunology 2022Quote: ... The membrane was washed three times with TBS-T for 10 min and subjected to the HRP-conjugated secondary goat anti-mouse antibody (Biolegend) in a solution of 3% non-fat milk in TBS-T for 1 h ...
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bioRxiv - Immunology 2022Quote: ... Placental neutrophils contained in the resulting single cell suspension were enriched via positive selection using 0.2μg anti-mouse Ly6G PE (Biolegend, clone 1A8) per 1×106 cells followed by anti-PE microbeads (Biolegend) ...
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bioRxiv - Immunology 2022Quote: Splenocytes isolated from four female mice (two Ezh2fl/fl and two CD19-Cre Ezh2fl/fl) were stained with BV421 anti-mouse CD19 antibody (Clone 6D5, BioLegend) and live/dead dye ...
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bioRxiv - Microbiology 2022Quote: ... cells were stained in PBS with 0.5% BSA with Fixable Viability Dye eFluor 780 (eBioscience) and a combination of the following antibodies: anti-mouse CD45 (PE/Cy5, 30-F11, BioLegend), CD45 (Alexa Fluor 700 ...
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bioRxiv - Immunology 2022Quote: The following antibodies were used to detect B cell populations in the BM: brilliant violet (BV) 421 anti-mouse CD19 antibody (Clone 6D5, BioLegend), PE/Cyanine5 anti-mouse/human CD45R/B220 (Clone RA3-6B2 ...
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bioRxiv - Pathology 2022Quote: ... The pellet was further washed once with FACS buffer (0.5% BSA/0.5 mM EDTA in PBS) and stained with APC-conjugated anti-mouse CD45 (1:300, BioLegend, CAT#103112), BV510-conjugated anti-mouse CD11b (1:300 ...
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bioRxiv - Immunology 2022Quote: Circulating CD20+ cells were depleted by intraperitoneal administration of 200 μg of rat-anti-mouse-CD20-depleting antibody (Biolegend, 152104) two days before the start of TAM administration ...
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bioRxiv - Neuroscience 2022Quote: ... according to manufactures protocol and blocked in 5% BSA/TBS for 1 h and incubated overnight at 4 °C with the following antibodies: mouse anti-CD9 (1:1000; Biolegend), mouse anti-CD63 (1:1000 ...
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bioRxiv - Physiology 2022Quote: ... CD11b+-SVF were incubated with were with 0.2 mg/mL of anti-mouse PE-F4/80 (BioLegend, #12-4801-82) with utilization of PE rat IgG2a K isotype Control (BioLegend#12-4321-80 ...
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bioRxiv - Immunology 2022Quote: Slices were placed on a microscopy slide and Fc receptors on cells were blocked to avoid nonspecific staining using 20 μl purified anti-mouse CD16/32 antibody (BioLegend) in 1X PBS with 2% FBS at a concentration of 20μg/ml for 30 min at 37 °C ...
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bioRxiv - Immunology 2022Quote: ... Intra-cellular labelling for detection of IL-10 was performed using the Intracellular Fixation & Permeabilization Buffer Set (eBioscience) and the PE mouse anti-human Anti-IL-10 mAb (Biolegend). Cells were kept at 4°C in 1.25% paraformaldehyde until analysis ...
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bioRxiv - Immunology 2022Quote: Cells were stained with the following surface marker antibodies: anti-mouse CD45 (BV510™, BioLegend, clone 30-F11, cat# 103138), anti-mouse CD11c (BV785™ ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Cytokines in frozen cell culture supernatant or frozen plasma were analyzed with a customized multiplex human or mouse cytokine Legendplex kit (BioLegend). Samples were measured at the MACS Quant Analyzer10 (Miltenyi Biotec ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and at passage 1 a depletion of CD45+ cells was performed using a Biotin anti-mouse CD45 Antibody (Biolegend, 103104) and Dynabeads™ Biotin Binder (ThermoFisher 11047) ...
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bioRxiv - Microbiology 2022Quote: ... using 1:1000 of polyclonal rabbit anti-human MCT1 IgG (Cell Signalling) and 1:1000 mouse anti-human CD147 IgG (Biolegend) followed by HRP-conjugated secondary IgG antibody of specific host type ...
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bioRxiv - Microbiology 2022Quote: ... Identity and purity of eluted proteins was assessed by coomassie staining on 10 % SDS PAGE followed by Western blotting with mouse anti-human basigin IgG (Biolegend). Fractions were pooled and concentrated using Amicon centrifugal filter units 100 kDa (Merck ...
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bioRxiv - Immunology 2022Quote: IL-2 ELISA was performed using an ELISA MAX™ Deluxe Set Mouse IL-2 as described in manufacturer’s protocol (BioLegend). The O.D ...
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bioRxiv - Cancer Biology 2024Quote: ... PL1 or KPL1 antibodies were incubated for 1 h followed by PE conjugated goat anti-mouse secondary antibody (cat. no. 405307, Biolegend) for another hour ...
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bioRxiv - Molecular Biology 2024Quote: ... The cell suspensions were then labeled with lineage related antibodies: PE/Dazzle™ 594 anti-mouse/human CD11b (Biolegend 101256), APC anti-mouse Ly-6G/Ly-6C (Gr-1 ...
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bioRxiv - Synthetic Biology 2024Quote: ... mice were intravenously injected with 20 µg of anti-mouse CD31 antibodies coupled with Alexa Fluor 647 (clone 390, Biolegend) to label blood vessels and ear was allowed to stabilize for 30 min ...
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bioRxiv - Neuroscience 2024Quote: ... and heavy non-phosphorylated neurofilament (NF-H) enriched in large pyramidal neurons (Anti-NF-H mouse monoclonal antibody, clone SMI32, 1:2000, Biolegend). All sections were counterstained with hematoxylin for further visualization of cell organization used in cytoarchitectonic type identification explained below and in Supplementary Table 1.
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bioRxiv - Immunology 2024Quote: ... Following volumes were added in a single tube containing 100ul FACS buffer per sample: 0.625uL per sample of APC/Cyanine7 anti-mouse CD19 (Biolegend 152412), 1.25uL per sample of Brilliant Violet 421™ anti-mouse CD38 (Biolegend 102732) ...
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bioRxiv - Pharmacology and Toxicology 2023Quote: ... Cells were counted and then incubated at 4 °C for 25 min with FITC anti-mouse CD45 (Biolegend, 103107, USA), Brilliant Violet 650 Ly6G (Biolegend ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were immunolabeled in blocking solution for 1 hour at RT with antibodies against the following proteins: ALIX at 5 μg/ml (mouse; no. 634502, Biolegend); IST1 at 0.4 μg/ml (rabbit ...
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bioRxiv - Immunology 2024Quote: ... FcγII/III receptors were blocked by prior 10 min incubation with Fc block (anti mouse CD16/32 antibody Clone 93, Biolegend) on ice.
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bioRxiv - Immunology 2024Quote: ... for 1 h at room temperature in the dark following pretreatment with Ultra-LEAF purified anti-mouse CD16/32 Ab (clone 93, BioLegend) for 30 min ...
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bioRxiv - Immunology 2024Quote: ... which were obtained from indicated sources: APC anti-mouse I-A/I-E (MHC II) (clone M5/114.15.2, BioLegend, 1:500), FITC anti-mouse I-A/I-E (MHC II ...
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bioRxiv - Immunology 2024Quote: ... The following antibodies were used for staining: Alexa Fluor 488 anti-mouse CD3 (17A2) (1:100) (BioLegend, San Diego, CA), PE anti-mouse CD4 (GK1.5 ...
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bioRxiv - Immunology 2024Quote: ... Cytokine concentration in these samples were determined using CBA mouse Inflammation Kit (BDTM) or ELISA according to the manufacturers’ instructions (Biolegend).
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bioRxiv - Immunology 2024Quote: ... cells were washed again and stained with anti-mouse antibodies to intracellular markers anti-TNF-α-Alexa Flour 700 (Biolegend, Alexa Fluor® 700 anti-mouse TNF-α Antibody ...
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bioRxiv - Immunology 2024Quote: ... and anti-dsDNA antibodies were detected with a 1/1000 dilution of alkaline phosphatase–conjugated goat anti-mouse IgG (BioLegend) for 1 hour at 37°C and developed with a phosphatase substrate for 30 min at 37°C.
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bioRxiv - Immunology 2024Quote: ... Mouse anti-human HLA-E (clone 4D12) primary antibody (MBL Lifescience) and then secondary antibody goat anti-mouse conjugated with APC (BioLegend) were applied with staining buffer (DPBS ...
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bioRxiv - Immunology 2024Quote: ... Naive CD8+ T cells were isolated by negative selection using the MojoSort Mouse CD8+ Naïve T cell Isolation Kit as per manufacturer’s instructions (BioLegend, >90% purity). Live cells were counted by trypan blue dye exclusion and resuspended at a concentration of 106 cells/mL in complete T cell media (RPMI-1640 (Cytiva ...
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bioRxiv - Microbiology 2024Quote: ... parasites were washed twice with FACS buffer and stained with (1:100) goat anti-mouse IgG-APC (BioLegend, cat# Poly4053). After incubation ...
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bioRxiv - Microbiology 2024Quote: Levels of cytokines and soluble mediators present in serum of infected mice were quantified using LEGENDplex Mouse Inflammatory Panel kit (BioLegend) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... CD4+CD62LhighCD44low/- T cells were purified from the spleen of wild-type BALB/c mice post staining with antibodies to mouse CD3 (17A2, Biolegend), CD4 (RM4-5 ...
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bioRxiv - Cell Biology 2023Quote: ... and cultured in DMEM/F-12 medium containing 10% FBS and 20 ng/mL mouse macrophage colony-stimulating factor (BioLegend) for 7 days ...
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bioRxiv - Microbiology 2024Quote: ... The amount of Ag-specific IgG was then quantitated by running a western blot using mouse IgG (BioLegend CAT#944803) of known mass as a standard ...
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bioRxiv - Molecular Biology 2023Quote: ... Conditioned medium was collected and IL-1β levels were assessed with ELISA MAXTM Deluxe Set Mouse IL-1β (Biolegend, 432604) following manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... Soluble His6-tagged M6G at varying concentrations was incubated for 10 min with HRP-conjugated mouse anti-His antibody (BioLegend) diluted 1:2000 in 0.1% BSA/TBST ...
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bioRxiv - Immunology 2023Quote: ... Wells were then incubated with 100 μl HRP-conjugated mouse anti-His antibody (1:2000 dilution in 1% BSA/TBST, BioLegend).
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bioRxiv - Pathology 2023Quote: ... followed by the surface monoclonal mouse anti-human conjugated antibodies: anti-CD15-PE (clone H198) and anti-CD11b-FITC (ICRF44) from Biolegend. Intracellular staining was performed after fixation and permeabilization with Fix/Perm kit (eBiosciences) ...
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bioRxiv - Immunology 2023Quote: ... with recombinant IL-15/IL-15RA complex (100 ng/ml; Thermo Fischer, Waltham, MA) or recombinant mouse IL-6 (50 ng/ml, BioLegend), TGF- β1 (1ng/mL) ...
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bioRxiv - Microbiology 2023Quote: ... Cells were re-suspended in flow cytometry buffer and incubated with TruStain FeX™ PLUS anti-mouse CD16/32 (BioLegend) (0.25 μg per 106 cells in 100 μL ...
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bioRxiv - Immunology 2023Quote: ... the cells were re-suspended in flow cytometry staining buffer (PBS containing 4% FBS and 2 mM EDTA) and blocked with TruStain FcX (mouse or human, Biolegend) for 5 min at 4°C ...