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Citations for BioLegend :
401 - 450 of 3163 citations for PD L1 Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Biochemistry 2022Quote: ... Cells were then washed once with ice-cold FACS buffer and incubated with 2 μg/mL PE anti-human IgG Fc (BioLegend) at 4 °C with gentle shaking in the dark for 1 hour ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were washed once with ice-cold FACS buffer and incubated with 1 μg/mL PE anti-human IgG Fc (Biolegend) at 4 °C with gentle shaking in the dark for 1 hour ...
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bioRxiv - Immunology 2022Quote: ... Cells were prepared for flow cytometry by washing once with PBS containing 2% HI-FBS followed by incubation with Brilliant Violet 421 anti-human IgG Fc Antibody (clone M1310G05) (Biolegend) or Brilliant Violet 421 Streptavidin (BioLegend ...
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bioRxiv - Immunology 2021Quote: ... resuspended in 100 µL PBE with a 1:100 dilution of biotin anti-human IgG Fc antibody (clone HP6017, BioLegend) for human serum or plasma derived antibodies or a 1:25 dilution of biotin goat anti-rat or anti-mouse IgG antibody (A16088 ...
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bioRxiv - Immunology 2020Quote: ... then cells were washed with 2% FCS-PBS and surface-stained with anti-human HLA-DR-Pacific Blue or -APC (clone L243; Biolegend) and anti-human CD123-FITC or -PeCy5 (clone 6H6 ...
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bioRxiv - Immunology 2021Quote: ... The plates were washed twice in FACS buffer and stained with 50 μl/well of 1:200 dilution of PE-conjugated mouse anti-human IgG Fc antibody (BioLegend) on ice in dark for 1h ...
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bioRxiv - Immunology 2021Quote: ... Cells were then blocked with human Fc-block (Miltenyi) in the presence or absence of purified anti-CD36 antibody (Clone 5-271; Biolegend) at 10 μg/ml for a further 15 min at 4°C ...
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bioRxiv - Molecular Biology 2023Quote: ... 10 million cells were resuspended in 1 mL of FACS buffer and 50 μL of Human TruStain FcX Fc Receptor Blocking Solution (BioLegend) was added and left to bind the cells on ice for 15 minutes ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... and resuspended in 100 μL Buffer B containing 100 nM M1 anti-FLAG antibody labeled with Alexa Fluor 488 (In house) and Alexa Fluor 647 anti-human IgG Fc (BioLegend) diluted 1:100 (v/v) ...
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bioRxiv - Microbiology 2023Quote: ... Cells were washed twice and resuspended for 30 minutes on ice in 1/250 anti-human IgG1-Fc-FITC (clone HP6017, BioLegend) and 1/1000 anti-hemagglutinin (HA ...
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bioRxiv - Immunology 2023Quote: ... Cells were then washed in FACS stain buffer and resuspended in diluted Human TruStain FcX Fc blocking reagent according to manufacturer’s instructions (Biolegend, USA) at 4□°C for 10 minutes protected from light ...
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bioRxiv - Immunology 2023Quote: ... Samples were then split into 7 FACS tubes and incubated with 100uL of diluted Human TruStain FcX Fc blocking reagent according to manufacturer’s instructions (Biolegend, USA) at 4□°C for 10 minutes protected from light ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were first blocked with 2% mouse serum (eBioscience San Diego, California) and Human Fc Block (Biolegend San Diego, California) for 10 minutes on ice ...
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bioRxiv - Genomics 2023Quote: ... Cells were pre-incubated with 5 µl of Human TruStain FcX™ (Fc Receptor Blocking Solution, BioLegend Cat. No. 422301) per 100µl of cell suspension for 5–10 minutes at room temperature ...
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bioRxiv - Immunology 2023Quote: ... were resuspended in labeling buffer (1% BSA in PBS) and underwent Fc blocking for 10 minutes on ice using Human TruStain FcX (BioLegend). Next ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were washed once with PBS and incubated in 50 µL PBS containing human Fc block (Supplementary Table 1) and Zombie Aqua viability dye (1:200, BioLegend) for 15 min at 4°C ...
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bioRxiv - Immunology 2019Quote: ... Fc-blocked (FcX, Biolegend) and stained for 30 minutes on ice with a lyophilized core antibody cocktail comprised of markers to allow identification of all major immune subsets (Supplementary Table 1) ...
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bioRxiv - Immunology 2024Quote: ... Fc-blocked (#158002, Biolegend), then stained for surface markers (B220 PE-Cy5 #103210 ...
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bioRxiv - Immunology 2024Quote: ... Fc-blocked (#158002, Biolegend), then stained for surface markers (CD45 APC #157606 and CD4 FITC #100406 ...
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bioRxiv - Immunology 2021Quote: ... The cells were then stained with PE-conjugated anti-Human IgG Fc antibody (clone M1310G05, BioLegend 410720, San Diego, CA, USA) to detect bound anti-CTLA-4 and with fluorescein isothiocyanate (FITC)-conjugated anti-CD27 (clone O323,
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bioRxiv - Bioengineering 2020Quote: ... cells were washed twice with wash buffer and then incubated with FITC-conjugated anti-cMyc antibody and PE-conjugated anti-human Fc (BioLegend, #342303) or PE-conjugated anti-Fab (Thermo Scientific ...
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bioRxiv - Cancer Biology 2021Quote: Wildtype RMC219 and RMC2C cells were harvested and 1 x 106 cells were resuspended in buffer A (PBS 1X, EDTA 2mM, inactivated FCS 1%) and 5uL of Human TruStain FcX (Biolegend, 422301) was added for 10 min at room temperature ...
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bioRxiv - Molecular Biology 2021Quote: ... incubated with an Alexa Fluor 647-labeled mouse monoclonal antibody against the human IgG1-Fc (Biolegend, San Diego, USA, cat #409320) and analyzed in a Gallios flow cytometer (Beckman Coulter ...
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bioRxiv - Cancer Biology 2021Quote: ... resuspended in 100 µl of FACS buffer and incubated in 5 µl of Human TruStain FcX™ (Fc receptor blocking solution, BioLegend) for 10 minutes at RT ...
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bioRxiv - Bioengineering 2022Quote: ... and resuspended in 100 μL of the FCM buffer with 5μL of the secondary Antibody (PE-anti human IgG Fc Recombinant, Biolegend, Catalogue 366903) and incubated for 30 minutes at 4°C. ...
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bioRxiv - Immunology 2021Quote: ... Cells were stained for 10 minutes at room temperature with Ghost Dye Violet 510 and Fc receptor blocking solution (Human TruStain FcX™, BioLegend) and washed once in FACS buffer ...
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bioRxiv - Immunology 2020Quote: ... once a week every week with IL-15 Receptor alpha Fc Chimera (R&D; 2.5μg/mouse) and recombinant human IL-15 (Biolegend, 5 μg/mouse). For immune blockade of PD-1 ...
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bioRxiv - Genomics 2021Quote: Both iMGs and oMGs were resuspended in staining buffer and were blocked with Fc receptor blocking antibody (Human TruStain FcX, BioLegend 422302) for 10 minutes ...
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bioRxiv - Bioengineering 2020Quote: ... Cells were washed twice with wash buffer and then incubated with FITC-conjugated anti-cMyc antibody and PE-conjugated anti-human Fc (BioLegend, #342303) or PE-conjugated anti-Fab (Thermo Scientific ...
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bioRxiv - Immunology 2022Quote: ... Blocking and antibody solutions were further supplemented with 1:150 Human TruStain FcX™ Fc Receptor Blocking Solution (BioLegend, London, UK), to reduce non-specific binding ...
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bioRxiv - Immunology 2023Quote: ... for 30 min at 4C in the dark before being incubated with Fc blocker (Human TruStain FcX, BioLegend, San Diego, California) in PBS with 1% BSA for 10 min at 4C ...
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bioRxiv - Immunology 2023Quote: ... Cells were rinsed with FACS buffer (PBS containing 0.5% BSA and 2mM EDTA) for 3 min at 300g and incubated with Fc Receptor Blocking solution (Human TruStain FcX™, Biolegend) for 10 minutes prior to cell surface staining with fluorescently labeled antibodies in Brilliant Stain buffer (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... for 30 min at 4C in the dark before being incubated with Fc blocker (Human TruStain FcX, Biolegend, San Diego, California) in PBS with 1% BSA for 10 min at 4C ...
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bioRxiv - Immunology 2023Quote: ... stained for 10 minutes at room temperature with Ghost Dye Violet 510 and Fc receptor blocking solution (Human TruStain FcX™, BioLegend), and washed once in FACS buffer ...
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bioRxiv - Immunology 2023Quote: ... buffer (PBS containing 2% FBS and 2 mM EDTA) and then incubated with human Fc receptor block (1:100 dilution, BioLegend, 422302) for 15 minutes on ice ...
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bioRxiv - Microbiology 2023Quote: ... and the Fc receptor was blocked with an anti-Fc receptor antibody (BioLegend). The cells were stained with a cocktail of antibodies against three surface makers ...
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bioRxiv - Immunology 2022Quote: ... PE-Cy7-labeled anti PD-1 (clone: RMPI-30; BioLegend), Super Bright 600–labeled anti-CD4 (Clone ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... anti-mouse PD-1 (Biolegend, cat#109121, clone#RMP1-30), anti-mouse CD19 (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... BV421-anti-PD-1 (29F.1A12) was purchased from BioLegend. Anti-CD16/CD32 FcBlock (2.4G2) ...
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bioRxiv - Cancer Biology 2022Quote: ... CD279-PECy7 (anti-PD-1, clone EH12.2H, ref. 329917, BioLegend), CD223-APC (anti-LAG-3 ...
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bioRxiv - Immunology 2022Quote: ... and anti-mouse PD-1 PE-Cy7 (29F.1, BioLegend).
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bioRxiv - Neuroscience 2023Quote: ... and/or 1.0 μg/mL PD-1 agonist (BioLegend; 758204) for 48 h ...
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bioRxiv - Bioengineering 2023Quote: ... anti-mouse CD279 (PD-1) PE/Cyanine7 (Biolegend, Cat: #109109), anti-mouse/human GL7 PE/Cyanine7 (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... anti-mouse CD279 (PD-1) (clone: RMP1-30, PE, Biolegend, cat# 109104 ...
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bioRxiv - Cell Biology 2023Quote: ... APCCy7-CD279 (PD-1) (clone 29F.1A12, Cat#135224, Biolegend).
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bioRxiv - Microbiology 2024Quote: ... PD-1 PE/Cy7 (clone RMP1-30; Biolegend Cat# 109110), CXCR-5 BV421 (clone L138D7 ...
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bioRxiv - Immunology 2024Quote: ... Brilliant Violet 421 anti-PD-1 clone 29F.1A12 (Biolegend), PE and Brilliant Violet 421 anti-ST2 clone DIH9 (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... PD-1-PE-Cy7(Clone EH12.2H7, BioLegend 329918, 1:30), CD3-Alexa Fluor 700 (Clone UCHT1 ...
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bioRxiv - Immunology 2020Quote: ... washed twice with cell staining buffer (0.5% bovine serum albumin/PBS) and incubated with 5 µl of human Fc receptor blocking solution (Trustain FcX, Biolegend, San Diego, CA) for 10 minutes at room temperature ...
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bioRxiv - Immunology 2020Quote: ... washed twice with cell staining buffer (0.5% bovine serum albumin/PBS) and incubated with 5 μl of human Fc receptor blocking solution (Trustain FcX, Biolegend, San Diego, CA) for 10 minutes at room temperature ...