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Citations for BioLegend :
451 - 500 of 3163 citations for PD L1 Human HEK293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: ... and bound antibodies were detected with a mouse IgG2a Alexa647-conjugated antibody directed against human IgG-Fc (BioLegend, San Diego, USA #409320). After washing the mean fluorescence intensities of transfected cells were determined with an Attune Nxt (Thermo Fisher Scientific ...
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bioRxiv - Immunology 2020Quote: ... the individual antibody hit at 5 μg/ml and/or ACE2-mFc was incubated with 1-2 million 1D8 spike+ cells and detected by anti-human-Fc PE (invitrogen, 12-4998-82) and FITC Goat anti-mouse-IgG (Biolegend, cat#405305) or APC Goat anti-mouse-IgG (Biolegend ...
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bioRxiv - Immunology 2020Quote: Cells were first incubated in 100 μL of PBS with 2mM EDTA for 15 min with 5μL Fc Block (Human TruStain FcX™, Biolegend, cat #422301) and a 1:500 dilution of Zombie UV viability dye (Biolegend ...
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bioRxiv - Microbiology 2023Quote: ... Nonspecific antigen binding to Fc receptors was inhibited via 10 minutes of blocking with Human TruStain FcX (BioLegend, San Diego, CA, USA). Cells were subsequently stained with mouse anti-human CD20 (Alexa-700) ...
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bioRxiv - Immunology 2023Quote: ... cells were resuspended in 90 μl of 1% BSA in PBS and Fc blocked with 10 μl Human Trustain FcX (Biolegend cat. 422302) for 10 minutes on ice then stained on ice for 45 minutes with a pool of 268 antibodies in 100 μl ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed and incubated in FACS buffer (1xPBS, 2%FCS) with the following anti-human antibodies: CD19-BV785 (BioLegend, 1:10), CD3-APC-eFluor 780 (Invitrogen ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed twice and resuspended in PBS containing 2% FBS before staining in the presence of Fc block (Miltenyi 130-059-901) with anti-human CD45 Alexa700 (clone HI30, BioLegend cat #304024) and anti-mouse CD45.1 PE-Cy7 (clone A20 ...
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bioRxiv - Neuroscience 2021Quote: ... The cells were blocked using an Fc blocking antibody (Biolegend Fc-block clone: 2.4G2) and lymphocytes were stained with CD3 and CD4 ...
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bioRxiv - Immunology 2021Quote: ... in PBS with 2% FCS and 2% EDTA (FACS buffer) with Fc block (Biolegend) for 25 minutes ...
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bioRxiv - Bioengineering 2019Quote: ... incubated with Fc Block (BioLegend) then with fluorochrome-conjugated antibodies (BioLegend unless noted ...
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bioRxiv - Immunology 2022Quote: ... anti-IgG Fc (Biolegend; M1310G05), anti-CD27 (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... Fc-blocking reagent (Biolegend, 422301) was used to block Fc receptor-introduced non-specific staining ...
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bioRxiv - Microbiology 2022Quote: ... CD16/32 (Fc block-Biolegend), B220 (AF700-Biolegend) ...
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bioRxiv - Neuroscience 2023Quote: ... Fc receptor blockade (Biolegend 101319), and anti-CD31 antibody (BD Biosciences 561073) ...
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bioRxiv - Genomics 2024Quote: ... Fc-blocking reagent (BioLegend, 422301) was used to block Fc receptor-introduced nonspecific staining ...
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bioRxiv - Immunology 2021Quote: ... PD-1 (29F.1A12) and Bcl-6 (K112-91) from BioLegend and BD Biosciences ...
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bioRxiv - Genomics 2019Quote: ... anti-PD-1 APC/Cy7 (clone EH12.2H7, cat. no. 329921, BioLegend), and anti-HLA-DR eVolve 605 (clone LN3 ...
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bioRxiv - Immunology 2020Quote: ... CXCR5 PE-Cy7 (MU5UBEE, eBioscience) and PD-1 BV421 (EH12.2H7, Biolegend). S-specific B cells were identified using biotinylated SARS-CoV-2 S probe conjugated to Streptavidin-PE (Thermo Fisher) ...
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bioRxiv - Immunology 2022Quote: ... α-PD-1 (BD Biosciences, 1:100, J43 or BioLegend, 29F.1A12), α-CXCR5 biotin (BD Biosciences ...
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bioRxiv - Cell Biology 2023Quote: ... anti-PD-1-APC (clone 29F.1a12, BioLegend 135209, diluted 200×), anti-B220-BV510 (clone RA3-6B2 ...
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bioRxiv - Immunology 2024Quote: ... PD-1-PE-Cy7 (Clone 29F.1A12, BioLegend 135216, 1:50), KLRG1-PE-dazzle (Clone 2F1/KLRG1 ...
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bioRxiv - Immunology 2024Quote: ... PD-1-PE-Cy7 (Clone 29F.1A12, BioLegend 135216, 1:50), KLRG1-PE-dazzle (Clone 2F1/KLRG1 ...
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bioRxiv - Immunology 2024Quote: ... PD-1-PE-Cy7 (Clone 29F.1A12, BioLegend 135216, 1:50), CD62L-Alexa 700 (Clone MEL-14 ...
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bioRxiv - Immunology 2024Quote: ... and in some experiments with APC-PD-1 antibody (BioLegend 329908). Data was acquired on SONY SA3800 spectral flow cytometer and analyzed using FlowJo software.
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bioRxiv - Microbiology 2023Quote: ... Fc receptors were blocked with 0.5% anti-CD16/32 Fc block antibody (Biolegend, clone 93) diluted in PBS containing 2% goat serum (Vector Laboratories ...
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bioRxiv - Biophysics 2022Quote: ... coverslips of each batch were incubated with 30 µg/ml anti-human IgG Fc (cross-reacts with rabbit) conjugated to Alexa Fluor-488 (Cat# 409321; BioLegend, San Diego, CA) for 45 minutes at room temperature in the dark ...
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bioRxiv - Neuroscience 2020Quote: ... in PBS and then incubated with an Fc receptor blocking solution (Human TruStain FcX, 1:20 in staining buffer; Biolegend, San Diego, CA) for 10 min ...
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bioRxiv - Cancer Biology 2023Quote: E0771 or E0771hPD-L1 cells were washed twice with ice-cold cell staining buffer (BioLegend) and stained with IgG control ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed in FACS buffer and stained on ice for 30 minutes with an Alexa647-labelled anti-human IgG-Fc antibody (Biolegend, San Diego, USA, #409320). Alexa647 mean fluorescence intensities were determined for GFP-positive HEK-293T cells with a FACS Attune Nxt and analyzed with the software Flow Logic™ (IInivai Technologies Mentone ...
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bioRxiv - Developmental Biology 2021Quote: ... and then Fc blocked (BioLegend, 101320), before staining with an antibody cocktail containing anti-MPO (Abcam ...
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bioRxiv - Cancer Biology 2019Quote: ... and Fc receptor blocking solution (Biolegend), before staining with fluorochrome-conjugated anti-human antibodies specific to anti-CD3 (UCHT1)/anti-CD45 (2D1) ...
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bioRxiv - Neuroscience 2022Quote: ... After fragment crystallizable (Fc) blocking (Biolegend), cells were stained for surface antigens (Extended Data Table 4) ...
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bioRxiv - Cancer Biology 2020Quote: ... After adding Fc receptor blocker (BioLegend), cells were labeled overnight at 4°C with a cocktail of antibodies recognizing chromatin modifications or intracellular components ...
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bioRxiv - Immunology 2020Quote: ... and incubated with FC block (BioLegend), followed by tetramer incubation (25μg/ml ...
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bioRxiv - Immunology 2022Quote: ... 5µg/ml CD80-Fc (555404, Biolegend), and 2µg/ml PD-L1-Fc (758206 ...
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bioRxiv - Immunology 2022Quote: ... stained with Fc receptor block (BioLegend) for 10 minutes at 4°C ...
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bioRxiv - Immunology 2023Quote: ... After Fc blocking (TruStain FcX; BioLegend), cells were stained with a metal-conjugated surface antibody cocktail (Table 2) ...
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bioRxiv - Cancer Biology 2023Quote: ... performed with Fc blocking (422302, BioLegend), and labelled with anti-CD138 (142508 ...
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bioRxiv - Immunology 2024Quote: ... and incubated with Fc Block (Biolegend) for 10 min at room temperature ...
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bioRxiv - Immunology 2021Quote: ... PD-1-AlexaFluor647 (29F.1A12) and Ter119-BV421 (TER-119) from BioLegend, BP-1-biotin (6C3) ...
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bioRxiv - Cancer Biology 2022Quote: ... T cell specific markers used were BV 421 PD-1 (BioLegend,135217), BV510 CD69 (BioLegend,104531) ...
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bioRxiv - Immunology 2022Quote: ... PD-1-PE-Cy7 (1:50; clone 29F.1A12; BioLegend Cat. 135216); Cxcr5-APC (1:50 ...
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bioRxiv - Immunology 2020Quote: ... CD279 (PD-1) BV650 (clone EH12.2H7, BioLegend, cat# 329950, dilution 2:100); CD45RA BV510 (clone HI100 ...
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bioRxiv - Immunology 2022Quote: ... CD4 (RM4-5; BV 711) and PD-1 (J43; FITC; all BioLegend). After fixation and permeabilization (Foxp3 Transcription Factor Staining Buffer Set ...
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bioRxiv - Cancer Biology 2023Quote: ... and PE anti-mouse CD279 (PD-1) Antibody (Biolegend, USA, Cat#135206). CD45 antibody is used to screen monocytes in cell suspension ...
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bioRxiv - Bioengineering 2020Quote: ... mice were treated with 200 μg of anti– PD-1 (Ultra-LEAF, BioLegend) via intraperitoneal (i.p. ...
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bioRxiv - Immunology 2020Quote: ... IL-17 (clone BL168) and PD-1 (clone EH12.2H7) were purchased from BioLegend, San Diego ...
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bioRxiv - Immunology 2021Quote: ... and PE/Cyanine7 anti-mouse CD279 (PD-1) antibody (BioLegend, catalog no. 135216) were then mixed with the cells and placed on ice for 30 min ...
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bioRxiv - Cancer Biology 2019Quote: ... PD-1 (BV605) and Tim-3 (BV421) which were all purchased from BioLegend for 1h on ice ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were labeled with the following antibodies: PD-1 FITC (1:50, Biolegend), NKp46-PE (1:50 ...