Acetaldehyde 3a 4 5 6 7 7a hexahydro 4 7 methano 1H inden 5 yl oxy citations for BioLegend

Quote: ... Cell surface expression of CTLA-4 was measured by adding anti-CTLA-4-PE-Dazzle594 mAb (BioLegend) in culture during restimulation for 24 h ...

The CD4 transmembrane GGXXG and juxtamembrane (C/F)CV+C motifs mediate pMHCII-specific signaling independently of CD4-LCK interactions

Mark S. Lee, et al., bioRxiv - Immunology 2023

Quote: ... were coated overnight at 4°C with 8μg of biotinylated anti-CD4 antibody (clone RM4-4, BioLegend). Prior to immunoprecipitation (IP) ...

Mouse Memory CD8 T cell subsets defined by Tissue Resident Memory (T_RM) Integrin Expression Exhibit Distinct Metabolic Profiles

Mike Sportiello, et al., bioRxiv - Immunology 2023

Quote: ... and plated in TRM-like culture media with 10 ng/mL mouse IL-7 (Biolegend Catalog No. 577804) and 10 ng/mL human TGFβ1 (Fisher Scientific PHG9214 ...

Circular single-stranded DNA is a superior homology-directed repair donor template for efficient genome engineering

Keqiang Xie, et al., bioRxiv - Bioengineering 2022

Quote: ... resuspended in fluorescence-activated cell sorting (FACS) buffer (2% FBS in PBS) and stained with 7-AAD (BioLegend), and the indicated cell-surface marker ...

Antagonism among DUX family members evolved from an ancestral toxic single homeodomain protein

Darko Bosnakovski, et al., bioRxiv - Molecular Biology 2023

Quote: Cells were trypsinized and stained with Annexin V and 7-AAD using APC Annexin V staining kit (BioLegend) according to the manufacturer’s instructions ...

A Gallium-68-Labeled Peptide Radiotracer For CD38-Targeted Imaging In Multiple Myeloma With PET

Ajay Kumar Sharma, et al., bioRxiv - Cancer Biology 2023

Quote: ... cells (1x106) were stained with PE-labelled anti-human CD38 monoclonal antibody (clone HB-7, BioLegend Cat # 356604) in FACS buffer (0.5% FBS with 2 mM EDTA ...

TESOGENASE, An Engineered Nuclease Editor for Enhanced Targeted Genome Integration

Hangu Nam, et al., bioRxiv - Bioengineering 2023

Quote: ... resuspended in fluorescence-activated cell sorting (FACS) buffer (2% BSA in PBS) and stained with 7-AAD (BioLegend) as a dye for cell viability assay ...

ALDH1A3-acetaldehyde metabolism potentiates transcriptional heterogeneity in melanoma

Yuting Lu, et al., bioRxiv - Cancer Biology 2024

Quote: ... This was centrifuged again (300xg for 7 minutes) and resuspended in 1X red blood cell lysis buffer (BioLegend) in deionized water ...

Most L1CAM is not associated with extracellular vesicles in human biofluids and iPSC–derived neurons

Vaibhavi Kadam, et al., bioRxiv - Neuroscience 2024

Quote: ... medium was changed on day 7 to fresh APEL medium supplemented with IL-3 (50 ng/ml) (BioLegend) and G-CSF (10 ng/ml ...

Catalytic inhibition of KAT6/KAT7 enhances the efficacy and overcomes primary and acquired resistance to Menin inhibitors in MLL leukaemia

Shellaina J. V. Gordon, et al., bioRxiv - Cancer Biology 2024

Quote: ... After 7-days treatment cells were stained with anti-CD11b antibody (Clone M1/70, Biolegend, Cat. 101212, RRID:AB_312794) and DAPI before analysis by flow cytometry ...

Aging trajectories of memory CD8⁺ T cells differ by their antigen specificity

Ines Sturmlechner, et al., bioRxiv - Immunology 2024

Quote: ... Cells were washed 3 times with PBS containing 2% FBS before 7-AAD viability staining solution (BioLegend, #420404) was added and cells were subjected to FACS (BD FACSAria 4-laser digital flow cytometer with FACSDiva v8 software) ...

HIV-1 Vpr drives a tissue residency-like phenotype during selective infection of resting memory T cells

Ann-Kathrin Reuschl, et al., bioRxiv - Microbiology 2021

Quote: ... Intracellular P-STAT5 staining was then performed in PBS with wash steps performed at 1800 rpm for 6 min at 4°C.The following antibody clones and fluorochromes were used: CD3 (UCHT1, Biolegend; Bv510, Bv711, FITC), CD8 (SK1 ...

Potent antitumor activity of a designed interleukin-21 mimic

Jung-Ho Chun, et al., bioRxiv - Biochemistry 2024

Quote: ... in RPMI complete medium in a 6-well plate that was pre-coated overnight at 4°C with anti-CD3 antibody (BioLegend Cat# 100340) at 100 pg/mL ...

Macrophage-Based iNos Reporter Reveals Polarization and Reprogramming in the Context of Breast Cancer

Javier A. Mas-Rosario, et al., bioRxiv - Immunology 2022

Quote: ... culture media was removed and replaced with media containing either 50 ng/mL interleukin 4 (IL-4) (BioLegend) for 48 hr to generate M2 macrophages or 50 ng/mL interferon-gamma (IFN-γ ...

Soluble CTLA-4 raises the threshold for T-cell activation and modulates anti-tumour immunity

Paul T. Kennedy, et al., bioRxiv - Cancer Biology 2023

Quote: ... and supplemented with sCTLA-4 conditioned media or murine CTLA-4-Fc fusion protein (10 µg/mL, BioLegend). After 4 days ...

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