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Citations for BioLegend :
201 - 250 of 2110 citations for Acetaldehyde 3a 4 5 6 7 7a hexahydro 4 7 methano 1H inden 5 yl oxy since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: 96-well flat-bottomed plates were pre-coated overnight at 4°C with 5 µg/ml anti-NKp30 (clone P30-15, Biolegend) and -CD18 (clone IB4 ...
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bioRxiv - Immunology 2021Quote: ... Splenocytes from uninfected or infected mice were incubated at 37C° in 5% CO2 for 4 h in medium containing Monensin and Brefeldin A solutions (Biolegend). Cells were then stained for surface antigens (TCRβ-PacificBlue and NK1.1-PE) ...
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bioRxiv - Immunology 2021Quote: Cell samples went through a series of centrifugations (400g, 5 minutes, at 4°C) and were stained with PE anti-CD45 (30-F11, BioLegend) in staining buffer (see flow cytometry section ...
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bioRxiv - Microbiology 2023Quote: ... for 5 minutes at 4°C and then fixed using commercial fixation buffer for 3 minutes (ref no. 420801, BioLegend). For intracellular staining of Granzyme B and Perforin ...
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bioRxiv - Molecular Biology 2024Quote: ... IP was performed overnight at 4° C using 5 μg antibody (anti-H3K9me3 Diagenode, ref. C15410056; anti-HA.11 BioLegend ref ...
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bioRxiv - Immunology 2024Quote: ... isolated naïve splenic B cells were cultured at a density of 0,5 x 106 cells/ml in the presence of 25 μg/ml LPS and 5 ng/ml IL-4 and stained with anti-CD138 (BioLegend). B cell proliferation was assessed by CellTrace Violet (Thermofisher ...
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bioRxiv - Pathology 2023Quote: ... Ldlr−/− mice fed a chow diet or HC diet for 4 days were anesthetized using Isoflurane and injected intravenously with 5 μg anti-mouse TIM4-APC antibody (RMT4-54, BioLegend). They were then euthanized 5 minutes after injection by cervical dislocation and perfused through the portal vein with PBS and then with 4% ice-cold PFA ...
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bioRxiv - Immunology 2023Quote: Single-cell suspensions were washed with cell staining buffer twice (300g, 5 min, 4⁰C) and incubated in 100 μl of Cell Staining Buffer (10 ml; 420201, Biolegend) with TruStain FcX™ (1:100 ...
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bioRxiv - Cancer Biology 2023Quote: ... and bone marrow were washed with cell staining buffer twice (300g, 5 min, 4⁰C) and incubated in 100 μl of Cell Staining Buffer (10 ml; 420201, Biolegend) with TruStain FcX™ (1:100 ...
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bioRxiv - Immunology 2023Quote: ... were derived from a cell sort that included an additional cell hashing step (TotalSeq-C0251/C0252/C0253/C0254/C0255 anti-human Hashtag 1/2/3/4/5 antibodies, cat # 394661/394663/394665/394667/394669, Biolegend) prior to B cell enrichment and staining ...
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bioRxiv - Immunology 2023Quote: ... Cells were sorted and purified cells were stained on ice for 30 min in PBS containing 1 % (w/v) BSA with hashtag antibodies (TotalSeq-B0301/B0302/B0303/B0304B0305 anti-mouse Hashtag 1/2/3/4/5, respectively, Biolegend). Cells were washed ...
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bioRxiv - Immunology 2024Quote: ... cells were centrifuged at 400 g for 5 minutes at 4°C and resuspended in cell staining buffer (CSB; Biolegend). The number of cells in the suspension was established using the CountessTM II cell counter (Invitrogen).
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bioRxiv - Neuroscience 2024Quote: ... centrifuged at 1500 rpm for 5 min at 4°C and resuspended in 500µl Cell Staining buffer (BioLegend, Cat#: 420201) containing 1:4000 dilution of SYTOX Blue (ThermoFisher ...
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bioRxiv - Neuroscience 2024Quote: ... centrifuged at 1500 rpm for 5 min at 4°C and resuspended in 500µl Cell Staining buffer (BioLegend, Cat#: 420201) containing 1:4000 dilution of SYTOX Blue (ThermoFisher ...
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bioRxiv - Neuroscience 2024Quote: ... centrifuged at 1500 rpm for 5 min at 4°C and resuspended in 500µl Cell Staining buffer (BioLegend, Cat#: 420201) containing 1:4000 dilution of SYTOX Blue (ThermoFisher ...
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bioRxiv - Neuroscience 2022Quote: ... The homogenates were first blocked in PBS with 10% rat serum with gentle rotation in a cold room and then stained for 1h protected from light at 4°C with CD206-FITC (cat no. 141704, Biolegend®) + MHCII-PE (cat no ...
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bioRxiv - Immunology 2022Quote: ... prM-DENV2-treated PBMCs were treated (1h, 37 °C) in the presence or absence of anti-TNF-α antibody (4 µg/mL, BioLegend). The TNF-α depleted supernatants were used to stimulate HUVEC for 6 hours and the expression of activation markers were analyzed by flow cytometry ...
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bioRxiv - Developmental Biology 2024Quote: ... blocked in 3% BSA/ 5% normal goat serum in PBS for 1h and incubated overnight at 4°C with primary antibodies (β-III tubulin/anti-tuj-1, 1/1000, Biolegend n°801202 ...
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bioRxiv - Immunology 2024Quote: ... The cells were expanded for additional 3 days and Siglec+ population was sorted by staining cells with anti-Siglec-7 APC (BioLegend, clone 6-434) or anti-Siglec-9 APC (BioLegend ...
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bioRxiv - Immunology 2020Quote: ... Annexin V/7-AAD staining kit was purchased from Biolegend (#640930).
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bioRxiv - Microbiology 2021Quote: ... and an anti-Siglec-1 7-239 Alexa 647 mAb (Biolegend). Cells were cytospun into coverslips ...
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bioRxiv - Cancer Biology 2020Quote: ... CD38 (HB-7, PE-Cy7, BioLegend, Cat no. 356608, 1:80) and HLA-DR (L243 ...
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bioRxiv - Cancer Biology 2022Quote: ... or 7-AAD Viability Staining Solution (BioLegend, San Diego, CA, USA). The combination of monoclonal antibodies used to identify T-cell subsets is described in the main text ...
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bioRxiv - Immunology 2022Quote: Pacific Blue Annexin V Apoptosis Detection Kit with 7-AAD (BioLegend) was used to characterize cell death by flow cytometry ...
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bioRxiv - Immunology 2022Quote: ... Dead cells were stained with 7-aminoactinomycin D (Biolegend, Cat# 420404). After incubation for 20 min on ice ...
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bioRxiv - Immunology 2022Quote: ... Dead cells were stained with 7-aminoactinomycin D (Biolegend, Cat# 420404). After incubation at 4°C for 20 min ...
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bioRxiv - Immunology 2022Quote: ... GL-7: PerCP-Cy5.5 (1:200; clone: 1D3, BioLegend Cat.152410); Fas-BV421 (1:300 ...
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bioRxiv - Immunology 2022Quote: ... Dead cells were stained with 7-aminoactinomycin D (Biolegend, Cat# 420404). After incubation for 20 min on ice ...
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bioRxiv - Physiology 2021Quote: ... APC Annexin V apoptosis detection kit with 7-AAD (Biolegend, #640930); Sytox™ Blue Dead Cell Stain for flow cytometry (ThermoFisher ...
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bioRxiv - Immunology 2023Quote: ... and Phycoerythrin Cyanine 7 (PE-Cy7) mouse anti-human CD11c (BioLegend). The cells were then incubated in the dark on ice for 20 min ...
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Cell therapy with IL-10-producing group 2 innate lymphoid cells suppresses Graft-versus-Host diseasebioRxiv - Immunology 2023Quote: ... 20ng/mL IL-7 and 20 ng/mL IL-33(BioLegend).
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bioRxiv - Neuroscience 2023Quote: ... Cells were also incubated with 7-AAD (Biolegend, USA, Catalog #420404) prior to sorting ...
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bioRxiv - Immunology 2024Quote: ... anti-CD4-APC-CY-7 (clone GK1.5) (BioLegend, SanDiego, CA, USA) (1:200) ...
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bioRxiv - Immunology 2024Quote: ... anti-CD107a-PE-Cy-7 (clone 1D4B) (BioLegend, SanDiego, CA, USA) (1:200 ...
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bioRxiv - Cancer Biology 2022Quote: ... the cells were resuspended in sorting buffer with 7-AAD (BioLegend) to gate the viable cells ...
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bioRxiv - Cancer Biology 2023Quote: ... PE/Cyanine 7 anti-mouse CD325 (Ep-CAM) antibody (118216, BioLegend); CD31 (PECAM-1 ...
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bioRxiv - Microbiology 2024Quote: ... before staining of total DNA with 7-AAD (BioLegend, Cat# 420404) for 15 minutes ...
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bioRxiv - Microbiology 2020Quote: ... 5 × 105 cells/mL of CD14+ enriched cells were stimulated using recombinant human (rh) GM-CSF and rhIL-4 (BioLegend, USA) at a concentration of each 500 IU/mL in RPMI1640 media supplemented with 10% FCS at 37°C in 5% CO2 ...
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bioRxiv - Cancer Biology 2022Quote: ... then incubated with their respective primary antibodies diluted in 5% skim milk in PBST at 4 °C overnight: purified mouse anti-βIII-tubulin (1:1,000) (BioLegend, cat. 801202), monoclonal mouse anti-GAPDH (1:50,000 ...
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bioRxiv - Immunology 2023Quote: ... cells were washed three times (300g, 5 min, 4⁰C) in cell staining buffer and kept in FluoroFix™ Buffer (422101, Biolegend) prior to run in a BD FACSymphony™ A5 Cell Analyzer (BD Biosciences) ...
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bioRxiv - Cancer Biology 2023Quote: ... cells were washed three times (300g, 5 min, 4⁰C) in cell staining buffer and kept in FluoroFix™ Buffer (422101, Biolegend) before run in a BD FACSymphony™ A5 Cell Analyzer (BD Biosciences) ...
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bioRxiv - Immunology 2022Quote: ... samples were pre-incubated for 5 minutes at 4°C with TruStain FcX™ (anti-mouse CD16/32) Fc block (clone 93; BioLegend). Samples were then stained for extracellular markers in the presence of Fc block for 30 minutes at 4°C protected from light using the following antibodies ...
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bioRxiv - Immunology 2024Quote: ... The fluorochrome-conjugated antibodies were as follows for flow cytometry: BV605 Rat Anti-Mouse CD4 (RM4-5/RM4-4; Biolegend #116027), PE-Cy™7 Rat Anti-Mouse CD8a (53-6.7 ...
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bioRxiv - Cancer Biology 2024Quote: ... or after a 4-day incubation period at 37°C with 5% CO2 (Day 4) following 30 min antibody staining and additional 15 min Annexin V APC binding (Biolegend, 640920). The antibodies used were as follow ...
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bioRxiv - Microbiology 2022Quote: ... unpulsed splenocytes and the S-peptide-pulsed splenocytes were stained with 0.5 μM and 5 μM (respectively) of 5-(and 6-) carboxyfluorescein diacetate succinimidyl ester (CFSE; BioLegend, San Diego, CA, USA). The unpulsed and peptide-pulsed splenocytes were mixed at a 1:1 ratio (5 × 106 cells each) ...
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bioRxiv - Microbiology 2020Quote: ... PBS supplemented with 0.1 µg/ml antifade-4 6-diamidino-2-phenylindole (DAPI) (BioLegend, USA) was added to the cells for at least 30 min before imaging ...
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bioRxiv - Bioengineering 2023Quote: ... and IL-4 and IL-6 using a LEGENDplex™ mouse inflammation cytokine assay (Biolegend). Collected blood was incubated at room temperature for 1 hr and centrifuged for 7 min at 12,000 g at room temperature ...
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bioRxiv - Immunology 2023Quote: ... The nuclei were stained with 3 µM DAPI (4’,6-diamidino-2-phenylindole; BioLegend, #422801) for 5 min ...
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bioRxiv - Immunology 2020Quote: ... Unbound antibodies were removed by washing with PBS were added at 5 × 104 cells/well in the presence of 4 μg/mL brefeldin A (Biolegend, Cat # 420601), 5 μg/mL GolgiStop (BD Biosciences ...
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bioRxiv - Cell Biology 2024Quote: S6K phosphorylation was measured by intracellular staining in murine CD8+ T cells activated for 24 h with plate-bound anti-CD3 (coated at 5 µg / ml for 14 h at 4 °C in PBS; BioLegend, Cat. # 100331) and 1 µg / ml soluble anti-CD28 (BioLegend ...