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Citations for BioLegend :
401 - 450 of 1106 citations for 4 4 Methylphenyl 1H pyrrole 3 carbonitrile since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... and incubated overnight at 4°C with the corresponding dilutions of the antibodies CD45 (BioLegend, clone 30-F11) and CD31 (Santa Cruz ...
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bioRxiv - Immunology 2021Quote: ... Naive P14 Thy1.1+ Il2ra−/− cells were isolated from 4-5 week old mice by depleting CD44hi cells (Biolegend biotin anti-mouse/human CD44 ...
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bioRxiv - Immunology 2021Quote: ... The following directly conjugated antibodies were used in these experiments: CD4 Alexa Fluor 700 (Clone RM4-4, BioLegend), TCRβ Alexa Fluor 700 (clone H57-597 ...
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bioRxiv - Immunology 2021Quote: ... Cells were also stained to measure activation with the following markers: CD137 APC (4:100; Biolegend Cat# 309810), OX40 PE-Cy7 (2:100 ...
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bioRxiv - Microbiology 2022Quote: Nunc 96-well maxisorp plates (Thermo) were coated overnight at 4°C with 100 ng of FcγRI (Biolegend) in coating buffer (0.1M sodium bicarbonate ...
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bioRxiv - Neuroscience 2024Quote: ... centrifuged at 1500rpm for 5mins at 4°C and resuspended in 500µl Cell Staining buffer (BioLegend, Cat#: 420201) containing 1:4000 dilution of SYTOX Blue (ThermoFisher ...
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bioRxiv - Neuroscience 2024Quote: ... centrifuged at 1500rpm for 5mins at 4°C and resuspended in 500µl Cell Staining buffer (BioLegend, Cat#: 420201) containing 1:4000 dilution of SYTOX Blue (ThermoFisher ...
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bioRxiv - Neuroscience 2024Quote: ... centrifuged at 1500rpm for 5mins at 4°C and resuspended in 500µl Cell Staining buffer (BioLegend, Cat#: 420201) containing 1:4000 dilution of SYTOX Blue (ThermoFisher ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated for 10 min at 4 °C with Fc receptor block (TruStain FcX, BioLegend, 1:10). Cells were washed and incubated in FACS buffer (1xPBS ...
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bioRxiv - Cancer Biology 2024Quote: Pre-coat a 12-well plate overnight at 4 °C using CD3 antibody (5 μg/ml, 317326, Biolegend), and before inoculation ...
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bioRxiv - Immunology 2023Quote: ... for 15 minutes at 4°C followed by incubation with AntiHA.11 Epitope Tag Affinity Matrix (BioLegend 900801) for 4 hours at 4°C under constant rotation ...
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bioRxiv - Immunology 2024Quote: ... in FACS buffer for 30 minutes at 4°C prior to being stained with Zombie NIR (Biolegend, #423105) for 15 minutes at room temperature ...
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bioRxiv - Immunology 2024Quote: ... Cells were cultured for 4-hours and then washed and stained with Zombie RedTM Fixable Viability dye (BioLegend). CountBright Absolute Counting Beads (ThermoFisher Scientific ...
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bioRxiv - Immunology 2024Quote: ... and stained for an additional 30 min at 4° C either with biotinylated anti-CD16 (clone 3G8, BioLegend) or anti-CD3 (clone UCHT1 ...
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bioRxiv - Immunology 2024Quote: ... and cells were surface stained at 4°C for 20 minutes: anti-F4/80:FITC (Biolegend, clone BM8), anti-CD64:PerCP-Cy5.5 (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... Surface markers were then stained for 20 minutes at 4°C with antibodies against TCRb-Fitc (Biolegend #109205), TCRb-PE-Cy7 (Biolegend #109221) ...
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bioRxiv - Cell Biology 2024Quote: ... then incubated overnight at 4 °C with either α-PS1 NTF antibody (1:2000; Biolegend PS1 NT1 823401) or α-PS2 NTF antibody (1:1000 ...
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bioRxiv - Cancer Biology 2024Quote: Cells were stained in suspension for 30 min at 4°C with anti-CD31-AlexaFluor 488 (Biolegend, 303110), anti-CD274-PE (BD ...
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bioRxiv - Immunology 2022Quote: ... for 1h and then stained overnight with the following directly conjugated mAbs: GFP AF488 (clone FM264G, BioLegend 338007), NK1.1 AF647 (clone PK136 ...
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bioRxiv - Immunology 2020Quote: ... Tim-3 clone RMT 3-23 (BioLegend) and clone FAB1529 (R&D) ...
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bioRxiv - Cell Biology 2020Quote: ... anti-mouse interleukin (IL)-4 PE (504104) and anti-mouse IL-17 PE (517008) (BioLegend, San Diego, CA, USA) were used ...
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bioRxiv - Developmental Biology 2021Quote: ... E14 cells were collected from culture dishes and incubated for 30 min at 4 °C with CITE-seq cell hashing62 antibody Ab_CD15 (1:200) (Biolegend). XEN-eGFP were collected from culture plates and incubated for 30 min at 4 °C with CITE-seq cell hashing antibody Ab_CD140 (1:200 ...
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bioRxiv - Developmental Biology 2021Quote: ... XEN-eGFP were collected from culture plates and incubated for 30 min at 4 °C with CITE-seq cell hashing antibody Ab_CD140 (1:200) (Biolegend). In the gastruloid sample ...
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bioRxiv - Immunology 2021Quote: ... The depleted cell fraction was then stained for 20 min at 4 °C with the CD45-PerCPCy5.5 antibody (1:50) (Biolegend) and the EpCAM-FITC antibody (G8.8 clone ...
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bioRxiv - Immunology 2021Quote: ... The depleted cell fraction was then stained for 20 min at 4 °C with a cocktail of fluorophore-labeled antibodies CD45-PerCPCy5.5 (1:50) (Biolegend), Ly51-PE (1:800 ...
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bioRxiv - Neuroscience 2021Quote: ... the membranes were incubated overnight at 4°C with anti-Aβ primary antibody (6E10 Biolegend, SIG-39320, 1:1000) following incubation with goat anti-mouse secondary antibody (Thermo Scientific ...
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bioRxiv - Immunology 2021Quote: ... Cells were incubated at 4°C for 15 minutes with an Fc receptor blocker (BioLegend TruStain FcX, cat # 422302) according to manufacturer instructions before cocktails of surface antibodies were added for 30 minutes at 4°C ...
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bioRxiv - Immunology 2021Quote: ... Samples were next stained for surface markers for 20min at 4°C with: PerCP-Cy5.5-conjugated anti-TCRβ (H57–597, BioLegend), APC-CY7-conjugated anti-B220 (RA3-6B2 ...
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bioRxiv - Immunology 2021Quote: ... about 4 × 106 cells were stained with 1 μl mouse Fc-block (anti-CD16/32; BioLegend, San Diego, CA) in 50 μl FACS buffer (PBS containing 2% FBS ...
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bioRxiv - Immunology 2021Quote: ... and stained with fluorochrome-labeled anti-mouse IFN-γ/TNF-α/IL-2/IL-4/IL-5 mAbs (Biolegend). LSRFortessa was used to acquire the flow cytometry data ...
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bioRxiv - Biochemistry 2020Quote: ... cells were cultured overnight with or without 4 μg/mL Syrian hamster anti-mouse CD28 (clone 37.51, BioLegend, USA) and plate-bound Armenian hamster anti-mouse CD3ε (clone 145-2C11 ...
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bioRxiv - Cell Biology 2020Quote: After coating with poly-L-Lysine, fluorodishes were coated overnight at 4 °C with αCD3ε (OKT3, #16-0037-85, eBioscience) + αCD28 (CD28.2, #302914 Biolegend), washed three times and pre-warmed at 37 °C for 10–15 min ...
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bioRxiv - Cancer Biology 2020Quote: ... we collected the beads and labeled them with 4 µg/ml PE anti-CD63 antibody (BioLegend, clone NVG-2) for 45 minutes at 37°C ...
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bioRxiv - Cancer Biology 2022Quote: ... or TFH mimicking signals (including 0.02 μg/mL recombinant human IL-4, Peprotech; 0.05 μg/ml recombinant human IL-21, Peprotech, and 5 μg/mL anti-human CD40, Biolegend). Complete RPMI medium contained RPMI 1640 (Gibco ...
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bioRxiv - Immunology 2022Quote: ... and stained for 30 min at 4°C with a fluorescein isothiocyanate (FITC)-conjugated anti-mouse CD4 Ab (BioLegend). The cells were then fixed ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated at 4°C overnight with the following primary antibodies: mouse anti-C3 (1:200 dilution, 846302, Biolegend), rabbit anti-CD31 (1:200 dilution ...
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bioRxiv - Immunology 2020Quote: ... then stained 15 minutes for surface markers and fixed for 20 minutes with 4% paraformaldehyde (PFA) in PBS (BioLegend). Cells were then permeabilized with 0.5 % saponin in Running buffer and stained for intracellular markers ...
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bioRxiv - Molecular Biology 2020Quote: ... 600 μL of WCE was incubated overnight at 4°C with 10μg of monoclonal RNA Poll antibody (Biolegend, USA), 2μg of anti-myc antibody (Sigma) ...
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bioRxiv - Immunology 2020Quote: ... Th1 cells were differentiated for 4 days in the presence of plate-bound αCD3 (5 μg/ml; Biolegend, #100339), rIL-12 (25 ng/ml ...
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bioRxiv - Pharmacology and Toxicology 2021Quote: ... Tissue was then blocked with 10% normal donkey serum/0.1% Triton X- 100/0.01% NaN3 followed by staining with Alexa Fluor 488 conjugated anti-human CD4 OKT 4) antibody (Biolegend) at 4° Celsius overnight (1:200 diluted in PBS) ...
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bioRxiv - Immunology 2020Quote: ... stained for 20 min at 4 °C in PBS/2% FCS in presence of Fc receptor blocking reagent (Biolegend) with anti-murine CD326-BV605 ...
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bioRxiv - Microbiology 2021Quote: ... Primary CD4+ T cells were activated for 4-5 days on T25 flasks coated with 5μg anti-CD3 antibody (clone OKT3, Biolegend) in the presence of 2μg/ml soluble anti-CD28 antibody (clone CD28.2 ...
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bioRxiv - Immunology 2020Quote: The following antibodies and reagents were used for flow cytometry: CD4 clone RM-4-5 (BD Biosciences, BioLegend, Tonbo), CD8α clone53-6.7 (BD ...
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bioRxiv - Immunology 2020Quote: ... Sections were stained at 4°C on an agitation stage overnight with anti-CD169 and anti-Ly6G antibodies (BioLegend). Stained sections were microscopically analyzed using Leica SP8 confocal microscope (Leica Microsystem ...
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bioRxiv - Cancer Biology 2022Quote: ... we collected the beads and labeled them with 4 μg/ml PE-labeled anti-CD63 antibody (BioLegend, clone H5C6) for 45 min at 37 °C ...
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bioRxiv - Immunology 2022Quote: ... enriched CD8 T cells were incubated for 10’ at 4°C in Staining buffer (2%BSA/0.01% Tween in PBS) containing FcBlock (1/10 dilution ;TruStainTM FcX, Biolegend) followed by a 30’ incubation in Staining Buffer containing TotalSeqB antibodies against Ly108 ...
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bioRxiv - Immunology 2022Quote: ... Samples were washed in PBS and were incubated at 4°C with CD16/32 Fc block (cat#101302, BioLegend) in PBS for 10 minutes before staining with antibodies (Supplementary Table 1 ...
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bioRxiv - Immunology 2024Quote: ... cells were harvested and stained for 30min at 4°C with anti-CD25 APC (Biolegend, Cat: 102012, 1:50), and ZombieUV (Biolegend ...
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bioRxiv - Genomics 2024Quote: ... The sorted cells were centrifuged at 400xg for 5mins at 4°C and re-suspended in 25μl Cell Staining Buffer (BioLegend). 2.5μl of 1:10 TruStain FcX™ PLUS (antimouse CD16/32 ...
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bioRxiv - Immunology 2024Quote: ... Stained spleen or LN samples were fixed with 1-4% phosphate-buffered paraformaldehyde solution (FluoroFix or Fixation Buffer, Biolegend). To detect transcription factors and certain intracellular proteins ...