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Citations for BioLegend :
451 - 500 of 1106 citations for 4 4 Methylphenyl 1H pyrrole 3 carbonitrile since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Single-cell suspensions were stained 30min at 4°C in the dark with PerCP/Cyanine5.5 anti-mouse Ly6G (clone:1A8, cat. no. 127615, BioLegend), FITC anti-mouse/human CD11b (clone:M1/70 ...
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bioRxiv - Immunology 2023Quote: ... Slides were then blocked and incubated overnight at 4°C with anti-Lysozyme antibody diluted 1:400 (Biolegend, #860001). Slides were washed and incubated with anti-rabbit Alexa Fluor 555 diluted 1:500 (Invitrogen ...
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bioRxiv - Immunology 2024Quote: ... Stained spleen or LN samples were fixed with 1-4% phosphate-buffered paraformaldehyde solution (FluoroFix or Fixation Buffer, Biolegend). To detect transcription factors and certain intracellular proteins ...
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bioRxiv - Immunology 2023Quote: ... Supernatant was discarded and cells were stained for 10 min at 4 °C with 2.5 μl Human TruStain FcX block (BioLegend) and 22.5 μl FACS buffer ...
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bioRxiv - Neuroscience 2023Quote: ... and then incubated overnight at 4 °C with primary antibodies directed against amino acids 6-18 (Tau 12, 1:1,000 dilution, ref: 806501, Biolegend), 159–163 (Tau HT7 ...
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The spindle protein CKAP2 regulates microtubule dynamics and ensures faithful chromosome segregationbioRxiv - Cell Biology 2023Quote: ... Primary incubation was done overnight at 4 °C with rabbit anti-CKAP2 1:2000 or mouse anti-GAPDH (BioLegend, 649201 ...
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bioRxiv - Immunology 2024Quote: ... cells were harvested and stained for 30min at 4°C with anti-CD25 APC (Biolegend, Cat: 102012, 1:50), and ZombieUV (Biolegend ...
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bioRxiv - Genomics 2024Quote: ... The sorted cells were centrifuged at 400xg for 5mins at 4°C and re-suspended in 25μl Cell Staining Buffer (BioLegend). 2.5μl of 1:10 TruStain FcX™ PLUS (antimouse CD16/32 ...
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bioRxiv - Cancer Biology 2022Quote: ... we collected the beads and labeled them with 4 μg/ml PE-labeled anti-CD63 antibody (BioLegend, clone H5C6) for 45 min at 37 °C ...
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bioRxiv - Immunology 2022Quote: ... enriched CD8 T cells were incubated for 10’ at 4°C in Staining buffer (2%BSA/0.01% Tween in PBS) containing FcBlock (1/10 dilution ;TruStainTM FcX, Biolegend) followed by a 30’ incubation in Staining Buffer containing TotalSeqB antibodies against Ly108 ...
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bioRxiv - Immunology 2022Quote: ... Samples were washed in PBS and were incubated at 4°C with CD16/32 Fc block (cat#101302, BioLegend) in PBS for 10 minutes before staining with antibodies (Supplementary Table 1 ...
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bioRxiv - Systems Biology 2022Quote: Sorted live single cells were centrifuged at 400 x g for 5min at 4°C and resuspended in 25μL of cell staining buffer (BioLegend). Human TruStain FcX FC blocking reagent (BioLegend ...
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bioRxiv - Neuroscience 2023Quote: ... Slides were incubated in primary antibody in 0.1 M Tris/2% FBS in a humidified chamber overnight at 4°C (SMI-32, Biolegend 801702 ...
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bioRxiv - Immunology 2024Quote: ... or 96-well plates precoated overnight at 4°C with 2 µg/ml anti-CD3ε antibody (145-2C11, BioLegend). For Th0 and Th17 differentiation ...
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bioRxiv - Immunology 2024Quote: ... Sections were then incubated overnight at 4°C with the primary antibody chicken anti-mouse Keratin 14 (Poly9060, BioLegend) at a 1:400 dilution in blocking buffer with anti-CD16/32 ...
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bioRxiv - Cancer Biology 2024Quote: ... Pluripotency marker expression was assessed every 10 passages by flow cytometry (PE anti-human SSEA-4 antibody, BioLegend, #330405) or RT-qPCR for DNMT3B ...
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bioRxiv - Bioengineering 2024Quote: ... anti-mouse IgG2a/b-FITC (5 g/ml) and anti-mouse CD138-PE (4 ug/ml; BioLegend cat# 142504); ii ...
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bioRxiv - Bioengineering 2024Quote: ... CD8+ T cells were stained overnight at 4°C using Alexa Fluor 647-conjugated anti-human CD8 antibody (BioLegend). Confocal imaging was performed using an inverted laser scanning confocal microscope ...
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bioRxiv - Cancer Biology 2024Quote: ... were incubated for 10 minutes at 4°C with TruStain FcXTM PLUS (clone S17011E, BioLegend, San Diego, CA, USA) to saturate Fc receptors ...
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bioRxiv - Cancer Biology 2024Quote: ... appropriate concentrations of specific antibodies were added for 30C:min at 4°C with 1µg/ml anti-CD31-AlexaFluor 488 (Biolegend, 303110), 1:800 anti-CD45-BUV805 (BD Biosciences) ...
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bioRxiv - Immunology 2020Quote: ... membranes were incubated with primary antibodies for 1h at RT with shaking (1:2000 anti-filaggrin clone Poly19058, Biolegend; 1:2000 anti-β-actin ...
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bioRxiv - Microbiology 2023Quote: ... for 1h at 25°C and then transferred to TBST supplemented with purified anti-HA antibody clone 16B12 (Biolegend) at a 1:2000 dilution for 1h at 25°C ...
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bioRxiv - Microbiology 2020Quote: ... Cleared lysates were transferred to new tubes and incubated overnight at 4°C with anti-GFP Nanobody Affinity gel (BioLegend). The affinity gel was then pelleted and washed twice using TEN buffer with 0.1% NP-40 and resuspended in equal volumes of NuPage LDS Sample Buffer (Thermo ...
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bioRxiv - Biophysics 2021Quote: ... DC were stained with PE CD45 (30-F11) and APC CD11c (N418) for 25 minutes at 4°C (all Biolegend). Excess antibodies were removed by washing with HBSS + 0.1% BSA ...
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bioRxiv - Immunology 2021Quote: ... Cells were stained directly for determining NK cell numbers and Ki67 staining or incubated for 4 hours in medium containing Brefeldin A (Biolegend) and Monensin (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... The sorted fraction was sequentially stained for 20 min at 4 °C with the Ly51-PE antibody (1:800) (Biolegend) and depleted of Ly51-positive cells using anti-PE Microbeads (Miltenyi Biotec) ...
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bioRxiv - Neuroscience 2021Quote: ... and incubated at 4□ overnight with the following primary antibodies: mouse anti-C3 (1:200 dilution; Biolegend cat. no. 846302), chicken anti-GFAP (1:300 dilution ...
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bioRxiv - Genomics 2022Quote: ... Cells were stained for the respective cell surface protein for 30 min at 4°C and measured by fluorescence-activated cell sorting (Sony SH800) (BioLegend: CD46 clone TRA-2-10 #352405 - 3μl per 1×106 cells ...
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bioRxiv - Immunology 2021Quote: ... The resultant cell suspension was stained with the following antibodies directed against cell surface antigens for 30 minutes at 4°C: CD45::BV421 (H130, BioLegend) and HLA-DR::PE-Cy7 (L243 ...
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bioRxiv - Immunology 2021Quote: ... cells stained with antibodies were distributed evenly and exposed to biotinylated trimer or peptide probes preincubated with streptavidin (30 minutes at molar ratio of 1:4 at 25°C) conjugated to PE (405203, BioLegend), BV421 (405226 ...
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bioRxiv - Immunology 2021Quote: FACS purified cell subsets were labeled by incubation for 30 minutes at 4°C with TotalSeq anti-mouse Hashtag antibodies (BioLegend), then washed ...
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bioRxiv - Immunology 2021Quote: ... 2×106 single splenocytes or BAL cells were incubated for 4 hours at 37° C in the presence of PMA/Ionomycin and Brefeldin A (Cell Activation Cocktail; BioLegend) or GolgiPlug control (BD Biosciences) ...
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bioRxiv - Immunology 2021Quote: ... Cells were washed in FACS buffer and fixed for 20 minutes at 4°C in 100 μl of Fixation Buffer (BioLegend).
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bioRxiv - Neuroscience 2020Quote: ... samples have been incubated overnight at 4°C with primary antibody anti β-amyloid (Aβ) (clone 6E10, 1:500, BioLegend) diluted in blocking buffer and stained with the corresponding goat secondary antibody ...
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bioRxiv - Genetics 2020Quote: ... and stained at 4°C in a final volume of 50µL with the following antibodies: CD14-FITC (1:50)(Biolegend 301803), CD16-PE (1:50)(Biolegend 3G8) ...
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bioRxiv - Cell Biology 2020Quote: Jurkat cells expressing both the GalT-GFP-SNAP and the HA-LAT encoding constructs were incubated for 30 min at 4°C on a wheel with 1:100 of mouse anti-HA Ab (901515; Biolegend). The cells were washed with cold PBS and incubated for 45 min at 4°C on a wheel with membrane-impermeable BG–PEG9–NHS29 ...
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bioRxiv - Cancer Biology 2022Quote: ... for 24h at 4°C on a shaker and then stained with antibodies (rat anti-CD3 [clone 17A2, cat#100202, Biolegend] ...
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bioRxiv - Immunology 2022Quote: ... or DC in vitro culture (5×105) were washed with PBS at 400 g for 5 min at 4°C and blocked with FcX (BioLegend) for 15 min ...
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bioRxiv - Neuroscience 2022Quote: ... Membranes were blocked in TBS-T/5% BSA for one hour at RT and incubated overnight at 4 °C with anti-P62 (SQSTM1) (1:500, #814802, BioLegend), anti-CD107b (LAMP-2 ...
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bioRxiv - Microbiology 2022Quote: ... and then stained for 30 minutes at 4℃ with the following antibody panel each diluted in PBS: APC/Cyanine7 anti-mouse CD45 antibody (Biolegend) or FITC anti-mouse CD45.2 antibody (Biolegend) ...
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bioRxiv - Cell Biology 2022Quote: ... bone marrow cells were incubated with cell surface markers for one hour at 4 °C and then washed with PBS before being resuspended with Annexin V binding buffer (Biolegend). Cells were then incubated with FITC Annexin V (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... fixed with 4% PFA for 15 minutes and subsequently stained for 30 minutes with anti-CD4-APC and anti-CD8-BV605 (Biolegend) in FACS buffer ...
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bioRxiv - Immunology 2020Quote: The cytokines IFNγ and TNFα contained in supernatants derived from 1-4 h BxPC3 - CAR T cell co-cultures were quantified by ELISA using Abs from Biolegend.
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bioRxiv - Immunology 2021Quote: ... Cell-free BAL fluid was stored at 4°C and processed within 24hr to measure cytokine/chemokine content using a LEGENDplex bead array kit (BioLegend).
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bioRxiv - Immunology 2020Quote: ... 2 × 105 WT or CD56-KO NK92 and YTS cell lines were added to 12-well flat-bottomed plates that were pre-coated overnight at 4°C with anti-NKp30 (clone P30-15, Biolegend) and -CD18 (clone IB4 ...
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bioRxiv - Immunology 2020Quote: 96-well flat-bottomed plates were pre-coated overnight at 4°C with 5 µg/ml anti-NKp30 (clone P30-15, Biolegend) and -CD18 (clone IB4 ...
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bioRxiv - Neuroscience 2021Quote: ... and finally incubated overnight at 4°C with primary antibodies against NKCC1 (T4, 1:1000, DSHB) and tubulin (anti-Tuj1, 1:5000, Biolegend) diluted in the blocking buffer with 0.1% tween ...
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bioRxiv - Cell Biology 2021Quote: ... for 10 min at 4°C and stained with fluorophore conjugated antibodies for 20 min at 4°C (anti-Pdpn PE-Cy7, Biolegend 127411; anti-Thy1 A647, Biolegend 105318 ...
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bioRxiv - Cell Biology 2021Quote: ... for 10 min at 4°C and stained with fluorophore conjugated antibodies for 20 min at 4°C (anti-Pdpn PE-Cy7, Biolegend 127411; anti-Thy1 A647, Biolegend 105318; anti-CD31 APC/Fire 750, Biolegend 102433 ...
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bioRxiv - Microbiology 2021Quote: ... and cells were stained for 30 min at 4°C with a combination of the following anti mouse immunophenotyping antibodies: from Biolegend: PacificBlue-conjugated anti-Ly-6C (clone HK1.4 ...