Labshake search
Citations for BioLegend :
4101 - 4150 of 4650 citations for Recombinant Mouse IL22 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
-
bioRxiv - Cancer Biology 2022Quote: ... and anti-CD20-APC for cells not incubated with protein or with anti-CD20-APC and anti-ROR1-PE (BioLegend) for samples treated with protein ...
-
bioRxiv - Immunology 2020Quote: ... the biotinylated proteins were incubated for a minimum of 1 h at 4°C with the streptavidin-conjugates AF647 (Biolegend), BV421 (Biolegend ...
-
bioRxiv - Immunology 2021Quote: Organ homogenates were thawed and the resulting supernatants analyzed for cytokines and proteins by ELISA (Biolegend, BD, and R&D) and Luminex (R&D ...
-
bioRxiv - Immunology 2021Quote: ... we processed PBMC samples for scRNAseq with paired measurements of 192 surface proteins (CITE-seq, using TotalSeq reagents from Biolegend). The list of the antibodies included in the TotalSeq custom panel are detailed in Supplementary Data 12 ...
-
bioRxiv - Immunology 2021Quote: ... Protein localization was confirmed by flow cytometry for either intact or fixed and permeabilized Intracellular Perm Buffer (BioLegend, Cat # 421002)) cells ...
-
bioRxiv - Immunology 2024Quote: ... in the presence of S2 mAbs (5 μg/mL), protein transport inhibitor cocktail (1:500, eBioscience) and anti-CD107a APC (clone H4A3, BioLegend). Antibody-dependent NK cell activation was allowed to occur for 4 hours at 37°C and 5% CO2 ...
-
bioRxiv - Immunology 2024Quote: ... and HRP-conjugated goat anti-mouse secondary antibody (1:3750 dilution) or a purified rat anti-SARS-CoV-2 spike protein S1 antibody (1:1000 dilution, BioLegend) and HRP-conjugated goat anti-rat secondary antibody (1:3000 dilution ...
-
bioRxiv - Immunology 2023Quote: ... Nucleocapsid level on exosomes was measured by ELISA using precoated ELISA plates (Legend Max SARS-CoV2 Nucleocapsid protein ELISA Kit, 448007, BioLegend) according to the manufacturer’s instructions ...
-
bioRxiv - Immunology 2023Quote: ... cells were incubated with Cell Stimulation Cocktail (plus protein transport inhibitors) (eBioscience) for 4 hrs or Cell Activation Cocktail (Biolegend) in the presence of Brefeldin A and Monensin for 5 hrs before staining.
-
bioRxiv - Immunology 2023Quote: ... Purified proteins were then verified by SDS-PAGE as well as by capturing proteins on streptavidin beads and immunocytometry with anti-CD40L and anti-His tag antibodies (Biolegend), and compared to commercial soluble CD40L (ACROBiosystems).
-
bioRxiv - Genomics 2022Quote: ... The custom oligos were synthesized by Integrated DNA Technologies and conjugated to anti-Nuclear Pore Complex Proteins antibody (Clone Mab414, BioLegend) by BioLegend ...
-
bioRxiv - Immunology 2023Quote: Biotinylated protein antigens were individually multimerized with fluorescently labeled streptavidin (BB515, BD Biosciences; BUV615, BD Biosciences; AF647, Biolegend; BV421, Biolegend) as described previously (25) ...
-
bioRxiv - Immunology 2023Quote: Biotinylated protein antigens were individually multimerized with fluorescently labeled streptavidin (BB515, BD Biosciences; BUV615, BD Biosciences; AF647, Biolegend; BV421, Biolegend) as described previously (25) ...
-
bioRxiv - Plant Biology 2024Quote: ... The protein levels were detected by immunoblot analysis using the primary antibodies: anti-HA antibody (Biolegend, #901503, dilution 1:5000), anti-FLAG antibody (CST ...
-
bioRxiv - Neuroscience 2024Quote: Aggregated α-synuclein was quantified in PBS- and TrX-soluble tissue fractions (5µg total protein/well) using the LEGEND MAXTM α-Synuclein Aggregate ELISA (BioLegend) according to manufacturer’s instructions.
-
bioRxiv - Immunology 2024Quote: ... for Fc receptors blocking and then simultaneously incubated with HpARI proteins tetramers and stained for CD45 (APC-Cy7, clone 30-F11; Biolegend). Cells were then acquired on a LSR Fortressa (BD ...
-
bioRxiv - Cancer Biology 2021Quote: NK cells were isolated from C57BL/6 mice (male and female) with the MojoSort mouse NK cell isolation kit (BioLegend) (purity >85 % based on the flow cytometric analysis with NK1.1 and CD49b antibodies) ...
-
bioRxiv - Immunology 2021Quote: ... and CD4+ CD25- and CD8+ T cells were purified using the MojoSort Mouse CD4 (supplemented with biotinylated anti-CD25) and CD8 T Cell Isolation Kits (BioLegend), respectively ...
-
bioRxiv - Immunology 2021Quote: ... The membrane was washed 3 times in TBST and incubated in TBST containing 5% non-fat dry milk and 1:1000 mouse anti-His (Biolegend) overnight ...
-
bioRxiv - Immunology 2021Quote: ... cells were subsequently blocked in flow buffer supplemented with either anti-mouse CD16/CD32 antibody (eBioscience) or Human TruStain FcX Fc Receptor Blocking Solution (BioLegend) for 30 min on ice ...
-
bioRxiv - Immunology 2021Quote: ... flow buffer was supplemented either with anti-mouse CD16/CD32 antibody (eBioscience) or Human TruStain FcX Fc Receptor Blocking Solution (BioLegend). The following antibodies were used for flow analysis ...
-
bioRxiv - Immunology 2021Quote: ... the cell suspensions were stained with the following antibodies: PE anti-mouse Ly-6C (BioLegend, 128008, clone HK1.4, 1:200), PerCP/Cyanine5.5 anti-mouse/human CD11b (BioLegend ...
-
bioRxiv - Immunology 2021Quote: ... total thymocytes were processed to single cell suspension in 10% FBS/HBSS (20×106 cells/ml) and incubated with rat anti-mouse CD24 antibodies (M1/69, Biolegend) (30 μg/100 ×106 cells ...
-
bioRxiv - Immunology 2021Quote: Cytokine levels in supernatants from stimulated cells were determined using the LEGENDplex™ Mouse anti-viral response panel (Biolegend, USA) as per manufacturer’s instructions and read on BD Accuri™ C6 Plus Flow Cytometer (BD Biosciences) ...
-
bioRxiv - Neuroscience 2021Quote: ... Sections were permeabilized and blocked with 0.1% Triton X-100 in PBS supplemented with 10% normal goat serum for 1 hour at room temperature and incubated with mouse anti-Tuj1 (1:300; BioLegend) overnight at 4°C ...
-
bioRxiv - Microbiology 2022Quote: IL-1 cytokine concentrations in the sera of mice or supernatants from cultured BMDMΦ were assessed using the Legendplex mouse inflammation kit (BioLegend) following the manufacturer’s instructions as described previously (42).
-
bioRxiv - Neuroscience 2021Quote: ... and incubated at 4□ overnight with the following primary antibodies: mouse anti-C3 (1:200 dilution; Biolegend cat. no. 846302), chicken anti-GFAP (1:300 dilution ...
-
bioRxiv - Cell Biology 2022Quote: ... 105 - 106 cells were resuspended in FACS buffer with 1 µg/ml TruStain FcXTM anti-mouse CD16/32 antibody (BioLegend) to block for 5 minutes before adding conjugated surface antibodies in FACS buffer at a 2X concentration ...
-
bioRxiv - Immunology 2022Quote: ... total lung tissue was marked with a lineage antibody (Lin, anti-mouse CD3ε, Ly-6G / Ly-6C, CD11b, CD45R / B220, TER-119 PE, Biolegend), anti-EpCAM for 10 minutes at temperature environment ...
-
bioRxiv - Immunology 2022Quote: ... total lung tissue was marked with a lineage antibody (Lin, anti-mouse CD3ε, Ly-6G / Ly-6C, CD11b, CD45R / B220, TER-119 PE, Biolegend), anti-CD45 (BD Bioscience ...
-
bioRxiv - Genomics 2020Quote: ... isolated single cells were co-stained in FACS buffer with FITC-conjugated mouse anti-human CD45 antibody (1:25; clone HI30, Biolegend), PE-conjugated mouse anti-human CD31 antibody (1:25 ...
-
bioRxiv - Immunology 2021Quote: ... and donor-derived CD4 T cells were analyzed by flow cytometry following magnetic bead–enrichment with MojoSort Mouse CD4 T Cell Isolation Kit (BioLegend).
-
bioRxiv - Immunology 2021Quote: ... CD4 T cells from the spleen and peripheral lymph nodes of C57BL/6 mice on 22-24 dpi with LCMV-c13 were harvested and enriched for CD4 T cells using a MojoSort Mouse CD4 T Cell Isolation Kit (BioLegend) prior to surface staining ...
-
bioRxiv - Immunology 2021Quote: FACS purified cell subsets were labeled by incubation for 30 minutes at 4°C with TotalSeq anti-mouse Hashtag antibodies (BioLegend), then washed ...
-
bioRxiv - Immunology 2021Quote: ... TCR expression was analyzed the next day by flow cytometry using an anti-mouse TCR antibody (H57-597, BV421, BioLegend).
-
bioRxiv - Immunology 2020Quote: Cytokine concentrations in supernatants of in vitro cultures were analyzed by the LegendPlex Mouse Th Cytokine Panel (13-plex) (BioLegend) according to the manufacturer’s instructions and analyzed on a FACS LSR II (BD Biosciences).
-
bioRxiv - Immunology 2019Quote: Splenic TFH and TFR cell were stained with antibodies against mouse CD4 (Clone: RM4-5, Biolegend, San Diego, CA, USA), CD44 (Clone:IM7 ...
-
bioRxiv - Immunology 2019Quote: ... mice were injected intraperitoneally with 100μg anti-TNFα (Ultra-LEAF™ Purified anti-mouse TNFα Antibody, clone MP6-XT22 Biolegend) or 200μg intraperitoneally (ip ...
-
bioRxiv - Immunology 2019Quote: ... IFNγ in serum was measured by ELISA according to the manufacturer’s instructions (Mouse IFN-γ ELISA MAX™ Deluxe, Biolegend). For both assays ...
-
bioRxiv - Immunology 2019Quote: The following antibodies were used for extracellular flow cytometry: anti-mouse CD45 BV650 (clone 30-F11, Biolegend, San Diego, CA), anti-mouse/human CD11b APC-Cy7 (clone M1/70 ...
-
bioRxiv - Immunology 2019Quote: ... (IRF4:rat monoclonal, eBiosciences, UK, mouse monoclonal: IRF8, eBiosciences, BATF, R&D Systems, JUN Millimark, UK, PU.1 Biolegend, UK). IRF1 staining was done using rabbit polyclonal anti-human IRF1 antibody (Abcam ...
-
bioRxiv - Immunology 2021Quote: ... Cells were then blocked in 10% BSA for 15 min and stained with 1:20 APC-anti-mouse IgD Ab (clone 11-26c.2a; 405713, BioLegend), FITC-anti-mouse IgM mAb (11-5790-81 ...
-
bioRxiv - Immunology 2021Quote: Mouse cytokines and chemokines were quantitated in graft and spleen tissue using the LEGENDplex Mouse Inflammation and custom plex panel (Biolegend) according to manufacturer’s instruction 22 ...
-
bioRxiv - Immunology 2021Quote: CD3+ T cells from lung single-cell suspensions (1×107 cells) were isolated by negative selection using the MojoSort mouse CD3+ T-cell Isolation kit (Biolegend) according to the manufacturer’s instructions ...
-
bioRxiv - Neuroscience 2021Quote: The following antibodies were used at the indicated concentrations (IHC-immunohistochemistry; ICC-immunocytochemistry; IB-immunoblot): purified anti-HA mouse (Biolegend Cat# 901501 ...
-
bioRxiv - Cancer Biology 2020Quote: ... This was followed by a 10 min incubation with anti-mouse Fc block and a 30 minute cell surface staining with anti-mouse CD4-APC/Cy7 and CD8-PE/Cy7 (Both from Biolegend). CFSE labeled CD8+ CTLs were subsequently sorted on a BD FACS Aria III (BD Biosciences ...
-
bioRxiv - Cell Biology 2021Quote: ... Platelet activation was detected by direct immunofluorescence labeling of alpha granule release marker CD62P (P-selectin) with mouse anti-human monoclonal antibody CD62P-AlexaFluor647 (Clone AK4, Cat.No. 304918, BioLegend, USA). and activation associated conformational change in integrin αIIbβ3 was detected with a mouse anti-human monoclonal antibody PAC1-FITC (Clone PAC-1 ...
-
bioRxiv - Microbiology 2021Quote: ... and the membranes were probed with a rabbit polyclonal antibody generated against the FHA mature C-terminal domain (MCD)(10) and a mouse monoclonal antibody generated against an HA epitope (BioLegend). Corresponding α-rabbit and α-mouse IRDye secondary antibodies (LI-COR Biosciences ...
-
bioRxiv - Microbiology 2021Quote: ... Plates were washed with water three times before applying 25 μL of secondary antibody (goat anti-mouse IgG Fc, BioLegend) at a dilution of 1:4,000 in blocking solution ...
-
bioRxiv - Cancer Biology 2020Quote: ... The homogenates were then resuspended in PBS buffer and stained with anti-mouse CD45.1 antibody conjugated with Brilliant Violet 421 (clone A20; BioLegend, 103134), CD11b monoclonal antibody conjugated with APC (M1/70 ...