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Citations for BioLegend :
3951 - 4000 of 4650 citations for Recombinant Mouse IL22 Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2021Quote: ... the cells were incubated for 1 h on ice in PE/Cy7 anti-mouse CD45.2 antibody (clone:104, BioLegend) and PE-conjugated anti-mouse IL1a antibody (clone:ALF161 ...
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bioRxiv - Neuroscience 2021Quote: ... Primary antibodies included in this study were: mouse IgG2a-anti-β-III-tubulin (Tuj1, 1:400, catalog #801202, Biolegend), chicken-anti-NF200 (1:1000 ...
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bioRxiv - Immunology 2021Quote: ... Secreted cytokines were measured with ELISA MAX Standard Set Mouse or Human kits according to the manufacturer’s protocol (BioLegend). Cellular viability was determined by resuspending cells in cRPMI with 0.1mM resazurin (Sigma Aldrich ...
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bioRxiv - Microbiology 2022Quote: ... The followed anti-mouse antibody panels were used for cell subset analysis: CD45 (clone 30-F11, Pe-Cy7, Biolegend), CD3e (clone 145-2C11 ...
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bioRxiv - Genomics 2020Quote: ... Tissues were immunostained with the primary antibodies rat anti-mouse CD31:Biotin (clone 390, Biolegend, San Diego, CA, USA) and Living Colors DsRed Polyclonal Antibody (rabbit anti-E2-Crimson ...
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bioRxiv - Immunology 2021Quote: ... for 30 min, washed with FACS buffer (PBS, 5% BSA, 0.05% sodium azide) and stained with anti-mouse IgG (Poly4053; Biolegend), IgA (Clone 11-44-2 ...
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bioRxiv - Immunology 2021Quote: ... about 4 × 106 cells were stained with 1 μl mouse Fc-block (anti-CD16/32; BioLegend, San Diego, CA) in 50 μl FACS buffer (PBS containing 2% FBS ...
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bioRxiv - Immunology 2019Quote: ... OT-I thymocytes were depleted of mature CD8 single positives using anti-human/mouse □7 integrin antibody (FIB504, Biolegend) and the EasySep Biotin Positive Selection Kit (Stemcell Technologies ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were incubated overnight at 4°C with mouse anti-HA primary antibody (1:1000, Anti-HA.11, Biolegend), washed 3× in PBS and incubated with Alexa-conjugated anti-mouse secondary antibody (1:500 ...
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bioRxiv - Synthetic Biology 2019Quote: ... Pre-conjugated primary antibodies used for flow cytometry and microscopy include: Pe/Cy7 anti-mouse CD274 (Biolegend cat: 124313), PE/Cy7 Rat IgG2b (Biolegend cat ...
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bioRxiv - Immunology 2019Quote: ... HBcAg and VSV-G antibodies listed above followed by rat monoclonal anti-mouse IgG1 conjugated to PE-Cy7 (BioLegend), goat anti-mouse IgG2a conjugated to AlexaFluor® 647 and goat anti-rabbit conjugated to AlexaFluor® 488 (ThermoFisher ...
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bioRxiv - Immunology 2019Quote: ... 0.2% BSA in PBS with 0.02% sodium azide), blocked with human (humans, NHPs) or mouse (mice) TruStain FcX block (Biolegend) for 10 minutes at room temperature with shaking ...
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bioRxiv - Immunology 2019Quote: The serum immunoglobulin levels were measured with LEGENDplex Mouse Immunoglobulin Isotyping Panel according to the manufacturer’s protocol (Biolegend, 740493).
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bioRxiv - Developmental Biology 2021Quote: ... Hosts were culled at 4 weeks and engraftment frequencies were estimated by flow cytometry using only naked mole-rat markers not cross-reactive with mouse cells and CD45.1 (A20, BioLegend). Engraftment rates were adjusted for input cell dose to 100k/Tx ...
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bioRxiv - Immunology 2021Quote: ... the cells were incubated for 5 min on ice with Fc Block (TruStain fcX anti-mouse CD16/32, Biolegend) and washed with 200 μL FACS buffer ...
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bioRxiv - Immunology 2021Quote: ... and stained with fluorochrome-labeled anti-mouse IFN-γ/TNF-α/IL-2/IL-4/IL-5 mAbs (Biolegend). LSRFortessa was used to acquire the flow cytometry data ...
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bioRxiv - Microbiology 2021Quote: ... resuspended in 100 μl FACS Buffer plus 5 μl TruStain fcX™ (anti-mouse CD16/32) (Biolegend Cat#101320), and incubated for 10 min on ice ...
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bioRxiv - Microbiology 2021Quote: ... Unspecific antibody binding was blocked by adding 10 μl TruStain fcX™ (anti-mouse CD16/32) (Biolegend, Cat#101320) to each sample and incubating for 10 min on ice ...
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bioRxiv - Microbiology 2021Quote: ... 400 μl cell suspension were incubated with 80 μl TruStain fcX™ (anti-mouse CD16/32) (Biolegend, Cat#101320) for 10 min on ice ...
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bioRxiv - Microbiology 2021Quote: ... Unspecific bindings were blocked by addition of 5 μl TruStain fcX™ (anti-mouse CD16/32) (Biolegend, Cat#101320) and incubation for 10 min on ice ...
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bioRxiv - Biochemistry 2020Quote: ... cells were cultured overnight with or without 4 μg/mL Syrian hamster anti-mouse CD28 (clone 37.51, BioLegend, USA) and plate-bound Armenian hamster anti-mouse CD3ε (clone 145-2C11 ...
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bioRxiv - Cancer Biology 2020Quote: ... Antibodies used for immunostaining: FITC-conjugated or Alexa Fluor 594-conjugated anti-mouse CD8 antibody (BioLegend, clone 53-6.7). Confocal microscopy was performed on a Zeiss LSM 780 microscope.
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bioRxiv - Cancer Biology 2020Quote: Single cell suspension obtained by trypsin treatment was washed and incubated with an anti-mouse CD326 (EpCAM) AlexaFluor 647 antibody (1:2000, clone G8.8, Biolegend). DAPI (10 μg/mL ...
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bioRxiv - Cell Biology 2021Quote: ... CD4+ T cells were then isolated by negative selection using the MojoSort Mouse CD4 T Cell Isolation Kit (Biolegend). Isolated CD4+ T cells were seeded at 1×106/mL in RPMI supplemented with 10% FBS ...
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bioRxiv - Neuroscience 2019Quote: ... followed by overnight incubation at 4°C with primary antibodies diluted in 1% NDS-PBS-Tx (1:1,000 for mouse anti-HA, #901514 BioLegend; 1:1,000 for rabbit anti-TOM20 ...
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bioRxiv - Neuroscience 2020Quote: ... cortical cells from embryonic mice were labelled with Brilliant Violet 421™ anti-mouse CD90.2 (Thy1.2) antibody (BioLegend, #140327). Cells were incubated with antibody at 4 °C for 20 minutes followed by washing with wash buffer (Ca2+/Mg2+ free PBS ...
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bioRxiv - Microbiology 2019Quote: ... Corresponding single-stained Ab isotype controls were also prepared (PerCP/Cy5.5 mouse IgG1, κ isotype (MOPC-21) from Biolegend; mouse IgG1 ...
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Naked Mole-Rat Hematopoietic Stem and Progenitors are Highly Quiescent with an Inherent Myeloid BiasbioRxiv - Cell Biology 2021Quote: ... 8 or 12 weeks and engraftment frequencies were estimated by flow cytometry using only naked mole-rat markers not cross-reactive with mouse cells and CD45.1 (A20, BioLegend). Engraftment rates were adjusted for input cell dose to 100k/Tx ...
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bioRxiv - Immunology 2022Quote: ... and stained for 30 min at 4°C with a fluorescein isothiocyanate (FITC)-conjugated anti-mouse CD4 Ab (BioLegend). The cells were then fixed ...
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bioRxiv - Cell Biology 2022Quote: ... The cell suspension was stained using allophycocyanin (APC)/Cy7-conjugated rat anti-mouse CD45 antibody (1:200, BioLegend, #101319), PE-conjugated rat anti-mouse EpCam antibody (1:500 ...
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bioRxiv - Immunology 2022Quote: ... Cell surface staining was performed for 30 min at 4ºC with fluorochrome-conjugated monoclonal antibodies to mouse antigens purchased from Biolegend: CD45 (30-F11) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Neurovascular inflammation was induced in mice via a unilateral injection of TNF-α (0.5 μg/mouse, 2.5 μL, BioLegend) into the striatum using a stereotaxic frame at the following coordinates relative to the bregma ...
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bioRxiv - Pathology 2022Quote: Serum levels of IL-6 and TNFα were measured by a mouse ELISA MAX Standard IL-6 kit (BioLegend) and a mouse ELISA MAX Standard TNFα kit (BioLegend).
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bioRxiv - Neuroscience 2022Quote: ... and incubated at 4°C overnight with the following primary antibodies: mouse anti-C3 (1:200 dilution, 846302, Biolegend), rabbit anti-CD31 (1:200 dilution ...
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bioRxiv - Microbiology 2022Quote: The HA epitope was detected with mouse monoclonal antibody (mAb) HA.11 (diluted 1:1000) (BioLegend, item no. 901515) or rabbit polyclonal antibody (pAb ...
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bioRxiv - Immunology 2022Quote: ... BMDMs supernatant and intracellular IL-12p70 were quantified by using ELISA MAXTM Deluxe Set Mouse IL-12 (p70) (Biolegend).
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bioRxiv - Immunology 2022Quote: ... Anti-Gr1-mediated neutrophil depletion: 10μg (female mice) or 20μg (male mice) of anti-Gr1/mouse (Ultra-LEAF, clone RB6-8C5, Biolegend, 108436) diluted in sterile low endotoxin PBS was injected intraperitoneally into mice ...
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bioRxiv - Immunology 2022Quote: ... The suspension of mouse CD4+ T cells and the spinal cord sections were stained with APC-Cy7-conjugated anti-mouse CD4 (clone GK1.5, Biolegend), BV510-conjugated anti-mouse CD4 (clone RM4-5 ...
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bioRxiv - Microbiology 2022Quote: Serum cytokine levels were determined using a beads-based immunoassay with a custom-designed mouse cytokine panel (LEGENDplex, BioLegend) following the manufacturer’s instructions ...
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bioRxiv - Pathology 2021Quote: The levels of serum TNF-α were determined using the LEGEND MAX Mouse TNF-α ELISA kit (Biolegend, CA) according to the manufacturer′s instructions.
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bioRxiv - Immunology 2020Quote: The serum IgE levels of the mice were measured using Mouse IgE ELISA MAX™ Deluxe (BioLegend cat. 432404) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Cells overexpressing Rab35 were detected using an anti-HA primary antibody (data not shown; Rabbit, Cell Signaling; Mouse, Biolegend) + Alexa Fluor-405 secondary antibody ...
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bioRxiv - Immunology 2020Quote: Non-specific binding of antibodies to Fc-receptors was blocked with anti-mouse CD16/CD32 (TruStain fcX from BioLegend) in single cell suspensions prepared from Spl ...
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bioRxiv - Immunology 2019Quote: ... OVA-specific IgG1 was detected using biotinylated anti-mouse IgG1 monoclonal antibody (0.02µg/mL RMG1-1, Biolegend – CA, USA) followed by SA-HRP (1:2000 dilution #P030701-2 ...
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bioRxiv - Pathology 2022Quote: ... cell suspensions were centrifuged again at 300 g for 5 min and then preincubated with 2.5 µg/ml Fc blocker (156604 for mouse, 422302 for human, BioLegend) on ice for 10 min ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies were: Syn303 [a mouse monoclonal antibody that recognizes residues 1-5 of human α-synuclein (50)] (BioLegend) at 1:4,000 ...
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bioRxiv - Immunology 2022Quote: ... sera were collected from mice blood and measured by the LEGENDplex mouse inflammation panel according to the manufacturer’s instructions (Cat#740446, BioLegend). Samples were read on the BD FACSymphony Flow Cytometer.
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bioRxiv - Microbiology 2019Quote: ... SERINC5 levels were assessed via C-terminal HA tag using the mouse monoclonal HA.11 (Biolegend, San Diego, CA) at 1 μg/ml in Odyssey blocking buffer (LI-COR Biotechnology ...
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bioRxiv - Bioengineering 2019Quote: ... and CD31+ cells were isolated using flow cytometry sorting after staining cells with anti-mouse CD31 antibody (Biolegend 102417) and plated onto Vitronectin-coated plates (1mL/well at 1µg/cm2 ...
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bioRxiv - Microbiology 2020Quote: IL-1β release was measured in murine serum using the LEGENDplex™ mouse inflammation panel bead-based immunoassay (BioLegend) and flow cytometry as described above except shaking was performed at 800 rpm.