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Citations for BioLegend :
3951 - 4000 of 4647 citations for Recombinant Mouse Cd40lg Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2022Quote: ... The followed anti-mouse antibody panels were used for cell subset analysis: CD45 (clone 30-F11, Pe-Cy7, Biolegend), CD3e (clone 145-2C11 ...
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bioRxiv - Genomics 2020Quote: ... Tissues were immunostained with the primary antibodies rat anti-mouse CD31:Biotin (clone 390, Biolegend, San Diego, CA, USA) and Living Colors DsRed Polyclonal Antibody (rabbit anti-E2-Crimson ...
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bioRxiv - Immunology 2021Quote: ... for 30 min, washed with FACS buffer (PBS, 5% BSA, 0.05% sodium azide) and stained with anti-mouse IgG (Poly4053; Biolegend), IgA (Clone 11-44-2 ...
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bioRxiv - Immunology 2021Quote: ... about 4 × 106 cells were stained with 1 μl mouse Fc-block (anti-CD16/32; BioLegend, San Diego, CA) in 50 μl FACS buffer (PBS containing 2% FBS ...
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bioRxiv - Immunology 2019Quote: ... OT-I thymocytes were depleted of mature CD8 single positives using anti-human/mouse □7 integrin antibody (FIB504, Biolegend) and the EasySep Biotin Positive Selection Kit (Stemcell Technologies ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were incubated overnight at 4°C with mouse anti-HA primary antibody (1:1000, Anti-HA.11, Biolegend), washed 3× in PBS and incubated with Alexa-conjugated anti-mouse secondary antibody (1:500 ...
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bioRxiv - Synthetic Biology 2019Quote: ... Pre-conjugated primary antibodies used for flow cytometry and microscopy include: Pe/Cy7 anti-mouse CD274 (Biolegend cat: 124313), PE/Cy7 Rat IgG2b (Biolegend cat ...
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bioRxiv - Immunology 2019Quote: ... HBcAg and VSV-G antibodies listed above followed by rat monoclonal anti-mouse IgG1 conjugated to PE-Cy7 (BioLegend), goat anti-mouse IgG2a conjugated to AlexaFluor® 647 and goat anti-rabbit conjugated to AlexaFluor® 488 (ThermoFisher ...
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bioRxiv - Immunology 2019Quote: ... 0.2% BSA in PBS with 0.02% sodium azide), blocked with human (humans, NHPs) or mouse (mice) TruStain FcX block (Biolegend) for 10 minutes at room temperature with shaking ...
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bioRxiv - Immunology 2019Quote: The serum immunoglobulin levels were measured with LEGENDplex Mouse Immunoglobulin Isotyping Panel according to the manufacturer’s protocol (Biolegend, 740493).
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bioRxiv - Developmental Biology 2021Quote: ... Hosts were culled at 4 weeks and engraftment frequencies were estimated by flow cytometry using only naked mole-rat markers not cross-reactive with mouse cells and CD45.1 (A20, BioLegend). Engraftment rates were adjusted for input cell dose to 100k/Tx ...
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bioRxiv - Immunology 2021Quote: ... the cells were incubated for 5 min on ice with Fc Block (TruStain fcX anti-mouse CD16/32, Biolegend) and washed with 200 μL FACS buffer ...
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bioRxiv - Immunology 2021Quote: ... and stained with fluorochrome-labeled anti-mouse IFN-γ/TNF-α/IL-2/IL-4/IL-5 mAbs (Biolegend). LSRFortessa was used to acquire the flow cytometry data ...
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bioRxiv - Microbiology 2021Quote: ... resuspended in 100 μl FACS Buffer plus 5 μl TruStain fcX™ (anti-mouse CD16/32) (Biolegend Cat#101320), and incubated for 10 min on ice ...
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bioRxiv - Microbiology 2021Quote: ... Unspecific antibody binding was blocked by adding 10 μl TruStain fcX™ (anti-mouse CD16/32) (Biolegend, Cat#101320) to each sample and incubating for 10 min on ice ...
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bioRxiv - Microbiology 2021Quote: ... 400 μl cell suspension were incubated with 80 μl TruStain fcX™ (anti-mouse CD16/32) (Biolegend, Cat#101320) for 10 min on ice ...
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bioRxiv - Microbiology 2021Quote: ... Unspecific bindings were blocked by addition of 5 μl TruStain fcX™ (anti-mouse CD16/32) (Biolegend, Cat#101320) and incubation for 10 min on ice ...
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bioRxiv - Biochemistry 2020Quote: ... cells were cultured overnight with or without 4 μg/mL Syrian hamster anti-mouse CD28 (clone 37.51, BioLegend, USA) and plate-bound Armenian hamster anti-mouse CD3ε (clone 145-2C11 ...
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bioRxiv - Cancer Biology 2020Quote: ... Antibodies used for immunostaining: FITC-conjugated or Alexa Fluor 594-conjugated anti-mouse CD8 antibody (BioLegend, clone 53-6.7). Confocal microscopy was performed on a Zeiss LSM 780 microscope.
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bioRxiv - Cancer Biology 2020Quote: Single cell suspension obtained by trypsin treatment was washed and incubated with an anti-mouse CD326 (EpCAM) AlexaFluor 647 antibody (1:2000, clone G8.8, Biolegend). DAPI (10 μg/mL ...
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bioRxiv - Cell Biology 2021Quote: ... CD4+ T cells were then isolated by negative selection using the MojoSort Mouse CD4 T Cell Isolation Kit (Biolegend). Isolated CD4+ T cells were seeded at 1×106/mL in RPMI supplemented with 10% FBS ...
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bioRxiv - Neuroscience 2019Quote: ... followed by overnight incubation at 4°C with primary antibodies diluted in 1% NDS-PBS-Tx (1:1,000 for mouse anti-HA, #901514 BioLegend; 1:1,000 for rabbit anti-TOM20 ...
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bioRxiv - Neuroscience 2020Quote: ... cortical cells from embryonic mice were labelled with Brilliant Violet 421™ anti-mouse CD90.2 (Thy1.2) antibody (BioLegend, #140327). Cells were incubated with antibody at 4 °C for 20 minutes followed by washing with wash buffer (Ca2+/Mg2+ free PBS ...
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bioRxiv - Microbiology 2019Quote: ... Corresponding single-stained Ab isotype controls were also prepared (PerCP/Cy5.5 mouse IgG1, κ isotype (MOPC-21) from Biolegend; mouse IgG1 ...
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Naked Mole-Rat Hematopoietic Stem and Progenitors are Highly Quiescent with an Inherent Myeloid BiasbioRxiv - Cell Biology 2021Quote: ... 8 or 12 weeks and engraftment frequencies were estimated by flow cytometry using only naked mole-rat markers not cross-reactive with mouse cells and CD45.1 (A20, BioLegend). Engraftment rates were adjusted for input cell dose to 100k/Tx ...
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bioRxiv - Immunology 2022Quote: ... and stained for 30 min at 4°C with a fluorescein isothiocyanate (FITC)-conjugated anti-mouse CD4 Ab (BioLegend). The cells were then fixed ...
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bioRxiv - Cell Biology 2022Quote: ... The cell suspension was stained using allophycocyanin (APC)/Cy7-conjugated rat anti-mouse CD45 antibody (1:200, BioLegend, #101319), PE-conjugated rat anti-mouse EpCam antibody (1:500 ...
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bioRxiv - Immunology 2022Quote: ... Cell surface staining was performed for 30 min at 4ºC with fluorochrome-conjugated monoclonal antibodies to mouse antigens purchased from Biolegend: CD45 (30-F11) ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: Neurovascular inflammation was induced in mice via a unilateral injection of TNF-α (0.5 μg/mouse, 2.5 μL, BioLegend) into the striatum using a stereotaxic frame at the following coordinates relative to the bregma ...
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bioRxiv - Pathology 2022Quote: Serum levels of IL-6 and TNFα were measured by a mouse ELISA MAX Standard IL-6 kit (BioLegend) and a mouse ELISA MAX Standard TNFα kit (BioLegend).
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bioRxiv - Neuroscience 2022Quote: ... and incubated at 4°C overnight with the following primary antibodies: mouse anti-C3 (1:200 dilution, 846302, Biolegend), rabbit anti-CD31 (1:200 dilution ...
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bioRxiv - Microbiology 2022Quote: The HA epitope was detected with mouse monoclonal antibody (mAb) HA.11 (diluted 1:1000) (BioLegend, item no. 901515) or rabbit polyclonal antibody (pAb ...
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bioRxiv - Immunology 2022Quote: ... BMDMs supernatant and intracellular IL-12p70 were quantified by using ELISA MAXTM Deluxe Set Mouse IL-12 (p70) (Biolegend).
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bioRxiv - Immunology 2022Quote: ... Anti-Gr1-mediated neutrophil depletion: 10μg (female mice) or 20μg (male mice) of anti-Gr1/mouse (Ultra-LEAF, clone RB6-8C5, Biolegend, 108436) diluted in sterile low endotoxin PBS was injected intraperitoneally into mice ...
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bioRxiv - Immunology 2022Quote: ... The suspension of mouse CD4+ T cells and the spinal cord sections were stained with APC-Cy7-conjugated anti-mouse CD4 (clone GK1.5, Biolegend), BV510-conjugated anti-mouse CD4 (clone RM4-5 ...
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bioRxiv - Microbiology 2022Quote: Serum cytokine levels were determined using a beads-based immunoassay with a custom-designed mouse cytokine panel (LEGENDplex, BioLegend) following the manufacturer’s instructions ...
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bioRxiv - Pathology 2021Quote: The levels of serum TNF-α were determined using the LEGEND MAX Mouse TNF-α ELISA kit (Biolegend, CA) according to the manufacturer′s instructions.
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bioRxiv - Immunology 2020Quote: The serum IgE levels of the mice were measured using Mouse IgE ELISA MAX™ Deluxe (BioLegend cat. 432404) according to the manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Cells overexpressing Rab35 were detected using an anti-HA primary antibody (data not shown; Rabbit, Cell Signaling; Mouse, Biolegend) + Alexa Fluor-405 secondary antibody ...
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bioRxiv - Immunology 2020Quote: Non-specific binding of antibodies to Fc-receptors was blocked with anti-mouse CD16/CD32 (TruStain fcX from BioLegend) in single cell suspensions prepared from Spl ...
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bioRxiv - Immunology 2019Quote: ... OVA-specific IgG1 was detected using biotinylated anti-mouse IgG1 monoclonal antibody (0.02µg/mL RMG1-1, Biolegend – CA, USA) followed by SA-HRP (1:2000 dilution #P030701-2 ...
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bioRxiv - Pathology 2022Quote: ... cell suspensions were centrifuged again at 300 g for 5 min and then preincubated with 2.5 µg/ml Fc blocker (156604 for mouse, 422302 for human, BioLegend) on ice for 10 min ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies were: Syn303 [a mouse monoclonal antibody that recognizes residues 1-5 of human α-synuclein (50)] (BioLegend) at 1:4,000 ...
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bioRxiv - Immunology 2022Quote: ... sera were collected from mice blood and measured by the LEGENDplex mouse inflammation panel according to the manufacturer’s instructions (Cat#740446, BioLegend). Samples were read on the BD FACSymphony Flow Cytometer.
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bioRxiv - Microbiology 2019Quote: ... SERINC5 levels were assessed via C-terminal HA tag using the mouse monoclonal HA.11 (Biolegend, San Diego, CA) at 1 μg/ml in Odyssey blocking buffer (LI-COR Biotechnology ...
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bioRxiv - Bioengineering 2019Quote: ... and CD31+ cells were isolated using flow cytometry sorting after staining cells with anti-mouse CD31 antibody (Biolegend 102417) and plated onto Vitronectin-coated plates (1mL/well at 1µg/cm2 ...
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bioRxiv - Microbiology 2020Quote: IL-1β release was measured in murine serum using the LEGENDplex™ mouse inflammation panel bead-based immunoassay (BioLegend) and flow cytometry as described above except shaking was performed at 800 rpm.
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bioRxiv - Physiology 2019Quote: CD4+CD25+ cells were isolated using negative selection of CD4+ T cells using the MojoSort Mouse CD4 Naïve T-cell Isolation Kit (catalog #480040, Biolegend) followed by positive selection of CD25+ ...
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bioRxiv - Immunology 2020Quote: ... Cells were resuspended at 20 ×106 cells/mL in Cell Staining Buffer and non-specific staining was then blocked by addition of 2μg/mL anti-CD16/32 (mouse TrueStain FcX Biolegend), and 2% rat serum (Invitrogen ...
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bioRxiv - Neuroscience 2020Quote: ... mouse monoclonal anti-Aβ (anti-β-amyloid 17-24, clone 4G8, #800712, 1:500, Biolegend, San Diego, CA, USA) overnight at 4°C ...