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Citations for BioLegend :
351 - 400 of 490 citations for Steroid Receptor Co Factor Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... Cells were stained with Zombie-violet or Zombie-NIR in PBS at 1:2000 dilution for 5 minutes at room temperature prior to resuspending in PBS + 2% HI-FBS + 2mM EDTA in the presence of Fc receptor blocking antibody (BioLegend, 101302) for blocking ...
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bioRxiv - Microbiology 2023Quote: ... Each well was blocked using 50 μL of FACS buffer (PBS + 5% FBS) + 2.5 μL Fc receptor (BioLegend, Cat No. 422302) block per well and then 150 μL FACS buffer was added and spun down ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were then stained for transferrin receptor expression by adding 0.25 μg of PE/Cy7 anti-human CD71 [CY1G4] (Biolegend, Cat# 334111) directly to the blocking solution for 30 min at room temperature ...
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bioRxiv - Immunology 2023Quote: ... Cells were rinsed with FACS buffer (PBS containing 0.5% BSA and 2mM EDTA) for 3 min at 300g and incubated with Fc Receptor Blocking solution (Human TruStain FcX™, Biolegend) for 10 minutes prior to cell surface staining with fluorescently labeled antibodies in Brilliant Stain buffer (BD Biosciences ...
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bioRxiv - Immunology 2023Quote: ... stained for 10 minutes at room temperature with Ghost Dye Violet 510 and Fc receptor blocking solution (Human TruStain FcX™, BioLegend), and washed once in FACS buffer ...
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bioRxiv - Immunology 2023Quote: ... buffer (PBS containing 2% FBS and 2 mM EDTA) and then incubated with human Fc receptor block (1:100 dilution, BioLegend, 422302) for 15 minutes on ice ...
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bioRxiv - Cancer Biology 2021Quote: ... Co-cultures were harvested and then stained with fixed viability dye (Zombie Aqua, Biolegend) and fluorochrome-labelled T-cell-specific antibodies targeting CD5 (clone 53-7.3 ...
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bioRxiv - Cell Biology 2020Quote: ... total co-culture cells were stained with an antibody raised against CD45.2 (104; Biolegend), and 25,000 FACS-sorted CD45.2+ cells were plated in duplicate wells of Methocult GF M3434 methylcellulose (StemCell Technologies ...
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bioRxiv - Cancer Biology 2022Quote: ... MM samples were co-stained with mouse anti-human CD138-Alexa647 (Biolegend, clone MI15). PLA was scored manually in CD138+ cells in a blinded fashion as either – or + ...
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bioRxiv - Physiology 2019Quote: ... co-IP experimental conditions using anti-C2CD5 or anti-HA (1:150, Biolegend, #901513) were used as described above.
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bioRxiv - Microbiology 2021Quote: ... Purified CD4+ T cells were co-stimulated with plate-bound CD3 antibody (Biolegend #300333) with media containing soluble CD28 (Biolegend #302943 ...
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bioRxiv - Molecular Biology 2019Quote: ... The sample was co-incubated with the mouse anti-Aβ 6E10 (BioLegend, 1:100) antibody and the rabbit anti-NUCB1 (Aviva Systems Biology ...
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bioRxiv - Neuroscience 2022Quote: ... Intrathecal injections of Inducible co-stimulatory (ICOS) agonist antibody (C398.4 Biolegend, 0.5 µg/µl) in total volume of 5 µl volume or PBS as vehicle control was administered using a 30-gauge (0.5’’ ...
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bioRxiv - Genetics 2022Quote: ... the sample was co-stained using an APC-conjugated CD34 (clone 581, 343509, BioLegend) to sort live CD66b-CD34+ and otherwise CD66b-bone marrow mononuclear cells ...
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bioRxiv - Immunology 2022Quote: ... After 4 days of co-culture cells were collected stained for CD8-PECy5 (BioLegend) and assessed for cell proliferation with flow cytometry ...
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bioRxiv - Immunology 2023Quote: ... These cells were then co-incubated with a mixture of LD-NIR (Biolegend, USA) and anti-mCD16/32 (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... 30μl of peptide-exchanged monomer and 3.3μl of PE-streptavidin (BioLegend Cat#405203) were mixed in a new plate ...
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bioRxiv - Cell Biology 2023Quote: ... PE-TGF-beta1 (latency-associated peptide/LAP) (clone TW7-20B9, Cat#141305; Biolegend), PE-CD278 (ICOS ...
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bioRxiv - Immunology 2019Quote: After isolation cells were treated with Fc receptor blocking solution and stained with anti-CD14-APC (2 μg/ml, 61D3; Biolegend, San Diego, USA), anti-CD41-PE (2 μg/ml ...
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bioRxiv - Immunology 2020Quote: ... washed twice with cell staining buffer (0.5% bovine serum albumin/PBS) and incubated with 5 µl of human Fc receptor blocking solution (Trustain FcX, Biolegend, San Diego, CA) for 10 minutes at room temperature ...
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bioRxiv - Immunology 2020Quote: ... washed twice with cell staining buffer (0.5% bovine serum albumin/PBS) and incubated with 5 μl of human Fc receptor blocking solution (Trustain FcX, Biolegend, San Diego, CA) for 10 minutes at room temperature ...
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bioRxiv - Microbiology 2021Quote: ... To assess ligands-receptor specificity to this interaction we performed these assays using anti-Cd11b antibody (Biolegend Cat # 101211, 10 μl/ml) and a non-PfEMP1-blocking anti-ICAM-1 antibody (Biolegend Cat # 322702 ...
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bioRxiv - Bioengineering 2022Quote: ... and then stained with the fluorescently labeled antibody that detects the corresponding cell surface receptor (anti-EGFR-BV421, Biolegend, Cat: 352911; anti-HER2-BV421, Biolegend, Cat: 324420; anti-EpCAM-AF647, Biolegend, Cat: 118211) at 4 ºC for >30 min ...
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bioRxiv - Neuroscience 2020Quote: ... Cell suspensions were incubated for 20 minutes on ice with anti-CD16/CD32 to block Fc receptors (1:50; BioLegend Cat #: 553141) and with a viability dye ...
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bioRxiv - Neuroscience 2022Quote: ... Cell suspensions were treated with anti-mouse-CD16/32 to block Fc receptors (BioLegend, 5 μg/mL, 20 min at 4 °C) before washing and transferring to PBS and treating with Zombie NIR (BioLegend ...
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bioRxiv - Microbiology 2023Quote: ... Nonspecific antigen binding to Fc receptors was inhibited via 10 minutes of blocking with Human TruStain FcX (BioLegend, San Diego, CA, USA). Cells were subsequently stained with mouse anti-human CD20 (Alexa-700) ...
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bioRxiv - Immunology 2023Quote: ... Cells were suspended in PBS + 2% HI-FBS + 2mM EDTA in the presence of Fc receptor blocking antibody (BioLegend, 101302, 1:400) and stained with antibodies at a 1:400 dilution against the following mouse markers ...
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bioRxiv - Cell Biology 2024Quote: ... Cells was washed with PBS and preincubated with 5 ml of TruStain FcX™ PLUS (anti-mouse CD16/32) Antibody (Fc Receptor Blocking Solution, BioLegend, 156603) for 10 minutes on ice to reduce nonspecific immunofluorescent staining ...
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bioRxiv - Immunology 2019Quote: ... the cells were fixed and permeabilized with a 1× dilution of True-Nuclear Transcription Factor kit (BioLegend) following the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... Intranuclear staining of Ki-67 was performed with the True-Nuclear™ Transcription Factor Buffer Set (BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: ... and M2 macrophages with 20ng/mL of macrophage –colony-stimulating factor (M-CSF, Cat. No. 574804, BioLegend). After 6 days ...
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bioRxiv - Immunology 2023Quote: ... After washing cells were fixed and permeabilized using the True-Nuclear™ Transcription Factor Buffer Set (BioLegend) followed by intracellular staining for 30 minutes at room temperature ...
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bioRxiv - Molecular Biology 2023Quote: ... 57,58 Bone marrow cells were cultured with 20 ng/ml of macrophage colony-stimulating factor (BioLegend #576404) for 7 days as previously described 58 to generate BMDMs ...
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bioRxiv - Immunology 2024Quote: ... cells were fixed and permeabilized using the True-Nuclear Transcription Factor Buffer Set (BioLegend Cat. No. 424401) using the manufacturer’s protocol with overnight fixation/permeabilization.
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bioRxiv - Immunology 2024Quote: ... When appropriate NK cells were fixed and permeabilised using the True-Nuclear Transcription Factor Buffer set (BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... were stained after cell fixation and permeabilization with True-NuclearTM transcription factor buffer set per protocol (BioLegend). Data were collected on BD LSR Fortessa or Beckman CytoFLEX flow cytometer and analyzed by FlowJo (Tree Star Inc. ...
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bioRxiv - Physiology 2023Quote: ... then seeded in 2mL of conditioned media with macrophage colony-stimulating factor (mCSF, 25 ng/mL, BioLegend). Five days later ...
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bioRxiv - Cancer Biology 2019Quote: ... YAP1 was co-stained with cytokeratin 5 (CK5) or 14 (CK14) (BioLegend, San Diego, CA) and imaged with a Nikon fluorescence microscope (Melville ...
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bioRxiv - Immunology 2020Quote: ... Six hours following co-incubation NR1 cells were harvested and stained for intracellular IFNγ (BioLegend) using an intracellular cytokine staining kit (BioLegend ...
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bioRxiv - Microbiology 2021Quote: ... or Lassa virus (LASV) GPC peptide pool together with 5μg/ml Brefeldin A (Biolegend). Following surface staining with Live/Dead-APC/Cy7 ...
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bioRxiv - Microbiology 2021Quote: ... or Lassa virus (LASV) GP peptide pool together with 5μg/ml Brefeldin A (Biolegend). Following surface staining with Live/Dead-APCCy7 ...
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bioRxiv - Immunology 2022Quote: ... or SARS-CoV-2 nucleocapsid peptide pool together with 5μg/ml Brefeldin A (Biolegend) for 16 hours ...
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bioRxiv - Microbiology 2023Quote: ... Membranes were probed with mouse monoclonal antibody generated against HA-tag peptide (YPYDVPDYA; BioLegend), rabbit polyclonal antibody generated against the entire FhaB mature C-terminal domain (FhaB residues 1981 to 2471 ...
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bioRxiv - Immunology 2019Quote: ... After isolation cells were treated with Fc receptor blocking solution and stained with anti-ICAM-1-PE (1 μg/ml, HA58; Biolegend, San Diego, USA), anti-VCAM-1-BV711 (0.5 μg/ml ...
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bioRxiv - Neuroscience 2020Quote: ... in PBS and then incubated with an Fc receptor blocking solution (Human TruStain FcX, 1:20 in staining buffer; Biolegend, San Diego, CA) for 10 min ...
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bioRxiv - Cell Biology 2022Quote: ... and fresh whole human blood diluted 1:5 in FACS buffer and supplemented with 5 ul of human TruStain Fc receptor blocker (Biolegend, San Diego, CA) for 5 mins on ice ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were then resuspended in 50 µl of flow buffer (1x PBS, 2% FBS, 10mM EDTA, 25mM HEPES) containing anti-CD16/CD32 antibody for Fc receptor blockade (Biolegend, 101320, 1:200) and incubated on ice for 5 minutes ...
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bioRxiv - Immunology 2022Quote: ... Cells were centrifuged for 5 min at 500 x g and 4 °C and stained with Fc receptor blocker (TruStain fcX™, BioLegend, 1:100) and viability stain (Zombie Aqua™ Fixable Viability Kit ...
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bioRxiv - Cancer Biology 2023Quote: ... NKG2D and DNAM-1 surface receptors on NK cells were blocked utilizing 25 μg/ml of purified anti-mouse NKG2D (Biolegend, clone CX5, 130202), purified anti-mouse DNAM-1 (Biolegend ...
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bioRxiv - Cancer Biology 2024Quote: ... Aliquots were thawed and incubated with the panel of antibodies following the blockade of non-specific binding of the immunoglobulin to the Fc receptors by TruStain FcX (BioLegend, San Diego, CA). LIVE/DEAD Fixable Near-IR Dead Cell Stain dye (Thermo Fisher Scientific ...