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Citations for BioLegend :
451 - 490 of 490 citations for Steroid Receptor Co Factor Peptide since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2021Quote: Biotinylated DPB1*04:01-monomers loaded with TFEYVSQPFLMDLE peptide (S167-180) were tetramerised using PE-Streptavidin (Biolegend, 405204). 1.1 volume PE-conjugated streptavidin was added to 1 volume of DPB1*04:01-monomer (1 mg/mL) ...
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bioRxiv - Cell Biology 2021Quote: ... Concentrations of tumor necrosis factor-alpha (TNF-α) and interleukin-6 (IL-6) were determined using ELISA kits (430907 and 431307, BioLegend, USA) according to the manufacturer’s instructions.
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bioRxiv - Physiology 2023Quote: Concentrations of tumour necrosis factor (TNF)-α from tissue culture supernatants post- stimulation were measured using ELISA MAX Standard Set Mouse TNF-α kit (Biolegend) according to the manufacturer’s protocol.
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bioRxiv - Genomics 2020Quote: ... isolated single cells were co-stained in FACS buffer with FITC-conjugated mouse anti-human CD45 antibody (1:25; clone HI30, Biolegend), PE-conjugated mouse anti-human CD31 antibody (1:25 ...
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bioRxiv - Microbiology 2021Quote: ... cells isolated from the lungs at 18 and 48 h post co-infection were also stained with either Ly6G clone 1A8 or RB6 clone RB6-8C5 (BioLegend) antibodies to confirm cell depletion ...
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bioRxiv - Bioengineering 2022Quote: ... Splenic cells at 1 × 106 were co-cultured with target cells at 1:2 effector to target ratio in the presence of anti-CD107a (Biolegend), brefeldin A and monensin and a serial dilutions (6x ...
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bioRxiv - Immunology 2022Quote: ... Cell samples at each timepoint were prepared as described and co-stained with PE-anti-CD19 and allophycocyanin-conjugated mouse anti-human CD23 (APC-anti-CD23, BioLegend, clone EBVCS-5 ...
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bioRxiv - Immunology 2020Quote: The cytokines IFNγ and TNFα contained in supernatants derived from 1-4 h BxPC3 - CAR T cell co-cultures were quantified by ELISA using Abs from Biolegend.
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bioRxiv - Immunology 2021Quote: ... Cell viability staining was routinely performed for plate stimulations and U87 co-culture using fixable violet or near-infrared viability dyes (Zombie UV fixable viability kit [Biolegend] ...
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bioRxiv - Immunology 2020Quote: ... Cell viability staining was routinely performed for plate stimulations and U87 co-culture using fixable violet or near-infrared viability dyes (Zombie UV fixable viability kit [Biolegend] ...
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bioRxiv - Bioengineering 2023Quote: IFNψ was measured from the supernatants of the co-culture experiments (TCE / CAR) using the colorimetric ELISA MAX Standard Set Human IFNψ kit (BioLegend) according to the manufacturer’s instruction ...
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bioRxiv - Cancer Biology 2023Quote: ... Supernatants were collected from effector-target cell co-cultures and analyzed for IFNy using either ELISA MAX Deluxe Human IFNγ kit (BioLegend) or Human IFNγ DuoSet ELISA (R&D Systems ...
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bioRxiv - Immunology 2023Quote: ... along with co-stimulatory antibodies (CD28 and CD49d at 1 μL/mL) and Brefeldin-A (1 μL/mL, BioLegend, USA). LIVE/DEAD and surface staining was performed as described above ...
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bioRxiv - Cancer Biology 2022Quote: ... Concentration of stable complexes following peptide exchange was quantified using the Flex-T HLA class I ELISA assay (Biolegend) per manufacturer’s instructions for HLA-A*02:01 ...
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bioRxiv - Immunology 2022Quote: ... or 2 μg/mL nucleocapsid protein (NP) peptide pool in the presence of 0.5 μg/mL anti-CD28 and anti-CD49d mAbs (Biolegend). Cells were incubated at 37°C for 9 hours and BFA was added at 3 h post incubation ...
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bioRxiv - Microbiology 2023Quote: ... MBL) or H-2q-restricted LCMV NP118 peptide (MBL) and 50 U/mL of recombinant mouse IL-2 (BioLegend) for 1 h ...
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bioRxiv - Cancer Biology 2023Quote: ... MEFs were trypsinized and stained with Apotracker-Green (Apo-15 peptide) and 7-AAD according to manufacturer’s instructions (Biolegend) to detect apoptotic cells ...
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bioRxiv - Genomics 2024Quote: ... were plated in a 96 well V-bottom plate (200,000 cells/well) and 1ug/mL peptides added to restimulate cells along with Monensin and Brefeldin A (Biolegend). Iberdomide (10nM ...
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bioRxiv - Immunology 2022Quote: ... TF and ICS was performed following permeabilization using True-Nuclear buffer kit according to the recommended protocol (True-Nuclear Transcription Factor Buffer Set; BioLegend, San Diego, CA). Samples were acquired on a Cytek Aurora (Cytek ...
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bioRxiv - Neuroscience 2020Quote: ... cells were treated with M-CSF (100ng/ml) Recombinant Human M-CSF (Macrophage-Colony Stimulating Factor, BioLegend cat. 574806, San Diego, CA, USA) for 5 min ...
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bioRxiv - Genomics 2021Quote: ... Cells were washed once in PBS and then fixed and permeabilized using the Foxp3/Transcription Factor Staining Buffer Set (eBioscience) before staining with PU.1-PE (BioLegend 7C2C34 cat #681307) or rat IgG2a-PE isotype (BioLegend RTK2758 ...
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bioRxiv - Cancer Biology 2021Quote: Cells were harvested at the indicated times and co-stained with Annexin-V-FITC and propidium iodide following manufacturer instructions (BioLegend, #640914). To assess active Caspase-3 ...
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bioRxiv - Cancer Biology 2022Quote: ... LEPs from co-cultures were separated after 10 days for gene expression qPCR analysis by FACS using anti-human CD133-PE (BioLegend, clone7) and anti-human CD271-APC (BioLegend ...
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bioRxiv - Cancer Biology 2023Quote: ... co-culture samples were collected at 120h and stained for live/dead fixable Aqua and CD3 (Clone HIT3a, Biolegend, Cat# 300306) in FACS buffer ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell suspensions were incubated with the peptides for 1 hour following fixation with formaldehyde and staining with cytochrome c antibody (BioLegend, Alexa Fluor® 647 anti-Cytochrome c - 6H2.B4 ...
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bioRxiv - Bioengineering 2021Quote: ... using a modified version of the commercial protocol as previously described.7 Concentration of stable complexes following peptide exchange was quantified using the Flex-T HLA class I ELISA assay (BioLegend) as per the manufacturer’s instructions ...
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bioRxiv - Immunology 2020Quote: ... biotinylated NP peptide loaded SLA monomers were freshly assembled into tetramer with streptavidin BV421 or BV650 (both from Biolegend, UK). Two million mononuclear cells were incubated with protease kinase inhibitor in PBS for 30 minutes at 37°C and tetramers added to the cells on ice for another 30 minutes ...
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bioRxiv - Immunology 2021Quote: ... cells were cultured in stimulation media without peptide stimulation or with phorbol 12-myristate 13-acetate (PMA) and ionomycin (BioLegend), respectively ...
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bioRxiv - Immunology 2020Quote: ... pooled from a minimum of four healthy human donors) and 50 ng/mL of recombinant human MΦ colony-stimulating factor (M-CSF) (Biolegend; San Diego, CA, USA). Monocytes were cultured in monocyte culture media at a cellular density of 1.5 x 106 cells/mL in 6-well cell culture plates ...
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bioRxiv - Immunology 2021Quote: ... NK were co-cultured for 8 h with target cells in the presence of CD107a PerCP-Cy5.5 antibody (H4A3, BioLegend, 250 ng/ml) and 2.5 ng/ml IL-15 (R&D) ...
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bioRxiv - Neuroscience 2021Quote: ... the cells were live-stained for two hours using a conjugated antibody targeted against an OVA257-264 peptide antigen (BioLegend, 141605). The cells were then fixed with 4% paraformaldehyde and imaged using the PerkinElmer Operetta CLS High-Content Analysis System ...
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bioRxiv - Microbiology 2020Quote: Splenocytes from intramuscularly vaccinated mice were incubated in culture with a pool of 253 overlapping 15-mer SARS-CoV-2 S peptides (Grifoni et al., 2020) for 12 h at 37°C before a 4 h treatment with brefeldin A (BioLegend, 420601). Following blocking with FcγR antibody (BioLegend ...
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bioRxiv - Microbiology 2023Quote: Splenocytes from intramuscularly vaccinated mice were incubated in culture with a pool of 253 overlapping 15-mer SARS-CoV-2 S peptides(25) for 12 h at 37°C before a 4 h treatment with brefeldin A (BioLegend, 420601). Following blocking with an anti-FcψR antibody (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... The levels of peptide-loaded MHC-I on the beads were tested using the PE-conjugated W6/32 antibody (Biolegend, 311406). Binding levels were measured in Luminex 100 Liquid Analyzer System and the results analyzed in GraphPad Prism v9.
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bioRxiv - Immunology 2024Quote: ... the harvested cells were restimulated using a pool of overlapping Wuhan Spike peptides (Miltenyi, 130-126-701) at 2 µg/mL for 16 hours in the presence of brefeldin A (Biolegend, #400602) at 5 µg/mL ...
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bioRxiv - Bioengineering 2021Quote: ... 1×106 P14 or pmel splenocytes were co-stained with Db-GP33 liposomes (10 μg/ml) and anti-mouse CD8-PE (Biolegend, Clone: 53-6.7) for 30 min on ice ...
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bioRxiv - Microbiology 2020Quote: ... After 48h of co-infection the cells were harvested and processed as above using an anti-MHC-II FITC-conjugated antibody (Biolegend San Diego, CA). Treatment with PMA (50 ng/ml ...
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bioRxiv - Immunology 2023Quote: Cytokine concentrations in supernatants harvested from CAR T cell: target cell co-cultures were determined by ELISA utilizing IFNγ and IL2 Deluxe kits (Biolegend; 431806 and 430106) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2022Quote: ... Cytokine staining of splenocytes after attLm-OVA infection was carried out after re-stimulation of CD8 T cell enriched splenocytes with 50 nM SIINFEKL peptide for 3 hours with Brefeldin A (Biolegend, cat#:420601).
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bioRxiv - Neuroscience 2023Quote: ... Extracellular deposits of Aβ peptide were immunostained with the mouse anti-β-amyloid 17-24 antibody (1:5000, clone 4G8, BioLegend, Koblenz, Germany). Alpha-synuclein pathology was detected with the anti-syn-1 antibody (1:2000 ...