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Citations for BioLegend :
351 - 400 of 819 citations for Borrelia burgdorferi C p Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: Splenocytes and peripheral lymph node cells were cultured for 5 h at 37°C in complete medium (1640 RPMI + 10% FBS) plus 0.5 μM OVA257-264 and Monensin solution (1,000×; Biolegend) for cytokines detection ...
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bioRxiv - Immunology 2024Quote: ... mast cells were stained using CD117 (c-Kit)-APC or APC/Cy7 and FcεRIα-PE (all from Biolegend) at 1:200 dilution for 20 min on ice ...
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bioRxiv - Neuroscience 2024Quote: ... centrifuged at 1500rpm for 5mins at 4°C and resuspended in 500µl Cell Staining buffer (BioLegend, Cat#: 420201) containing 1:4000 dilution of SYTOX Blue (ThermoFisher ...
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bioRxiv - Neuroscience 2024Quote: ... centrifuged at 1500rpm for 5mins at 4°C and resuspended in 500µl Cell Staining buffer (BioLegend, Cat#: 420201) containing 1:4000 dilution of SYTOX Blue (ThermoFisher ...
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bioRxiv - Neuroscience 2024Quote: ... centrifuged at 1500rpm for 5mins at 4°C and resuspended in 500µl Cell Staining buffer (BioLegend, Cat#: 420201) containing 1:4000 dilution of SYTOX Blue (ThermoFisher ...
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bioRxiv - Systems Biology 2024Quote: ... Permeabilization was performed at -20 °C for an hour with the True-Phos™ Perm Buffer from Biolegend. Staining was performed with FITC-coupled mouse monoclonal anti-ERK1/2 phospho (Thr202/Tyr204 ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated for 10 min at 4 °C with Fc receptor block (TruStain FcX, BioLegend, 1:10). Cells were washed and incubated in FACS buffer (1xPBS ...
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bioRxiv - Cancer Biology 2024Quote: Pre-coat a 12-well plate overnight at 4 °C using CD3 antibody (5 μg/ml, 317326, Biolegend), and before inoculation ...
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bioRxiv - Immunology 2019Quote: ... then incubated with antibodies specific for intracellular proteins and Brilliant Violet 605-streptavidin conjugate (BioLegend) to detect biotin-conjugated antibodies ...
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bioRxiv - Cell Biology 2019Quote: ... The proteins were analyzed by SDS-PAGE and immunoblotting with anti-GST (BioLegend MMS-112P) and anti-His (Qiagen 34660 ...
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bioRxiv - Immunology 2020Quote: Interferon beta (IFNβ) protein levels in plasma were measured using Legendplex Mouse Inflammation Panel (Biolegend).
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bioRxiv - Immunology 2021Quote: ... Bio-PyCSP protein (38.3 kDa) at 16 μM was combined with SA-APC antibody (Biolegend) at 2 μM ...
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bioRxiv - Immunology 2021Quote: The following antibodies have been used to stain extracellular proteins: anti-CD8-Alexa488 (Biolegend 100723), anti-PD1-PE (eBioscience 12-9981-83) ...
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bioRxiv - Microbiology 2019Quote: ... The following antibodies were used for detection of proteins of interest: HA.11 (mouse, Biolegend), β-actin (mouse ...
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bioRxiv - Biochemistry 2021Quote: ... The proteins were analyzed by SDS-PAGE and immunoblotting with anti-GST (BioLegend MMS-112P) and anti-His (Qiagen 34660 ...
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bioRxiv - Immunology 2021Quote: ... Samples were thawed and proteins quantified using a LEGENDplex Human Proinflammatory Chemokine Panel immunoassay (BioLegend) according to the manufacturer’s instructions ...
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Co-stimulatory molecules decide T cell fate through regulations of their invigoration and impairmentbioRxiv - Molecular Biology 2022Quote: Synchronized EL4 cells were stimulated by Ig Protein with anti-CD3 (final 2μg/ml, Biolegend) conjugated magnetic beads ...
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bioRxiv - Immunology 2022Quote: ... endogenous biotin and avidin proteins were successively blocked using an Avidin/Biotin Blocking Kit (Biolegend), for 10 min each ...
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bioRxiv - Neuroscience 2022Quote: ... The following antibodies were utilized for immunoblotting: myelin basic protein (MBP; Biolegend; 808401; 1:1000), PLP (rat hybridoma ...
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bioRxiv - Immunology 2024Quote: ... and FcεRIγ (FCABS400F antibody, Milli-pore)10 protein expression on live (Zombie red, 77475; BioLegend), CD3-negative (300318 antibody ...
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bioRxiv - Immunology 2024Quote: The following antibodies have been used to stain extracellular proteins: BV711 anti-CD45RA (Biolegend 304138), APC anti-CD45RO (Biolegend 304210) ...
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bioRxiv - Cell Biology 2019Quote: ... Fixed and blocked hMPCs were incubated overnight at 4°C with an anti-BrdU antibody (1:400 dilution, Biolegend) followed by PBS washes and a 60 min incubation with an Alexa-Fluor 488 conjugated anti-mouse secondary antibody (Invitrogen) ...
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bioRxiv - Immunology 2021Quote: ... were co-incubated with 6×105 target cells expressing mCherry at 37°C for 2 hours in the presence of 2µM monensin (BioLegend). The cells were centrifuged ...
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bioRxiv - Developmental Biology 2021Quote: ... E14 cells were collected from culture dishes and incubated for 30 min at 4 °C with CITE-seq cell hashing62 antibody Ab_CD15 (1:200) (Biolegend). XEN-eGFP were collected from culture plates and incubated for 30 min at 4 °C with CITE-seq cell hashing antibody Ab_CD140 (1:200 ...
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bioRxiv - Developmental Biology 2021Quote: ... XEN-eGFP were collected from culture plates and incubated for 30 min at 4 °C with CITE-seq cell hashing antibody Ab_CD140 (1:200) (Biolegend). In the gastruloid sample ...
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bioRxiv - Immunology 2021Quote: ... The depleted cell fraction was then stained for 20 min at 4 °C with the CD45-PerCPCy5.5 antibody (1:50) (Biolegend) and the EpCAM-FITC antibody (G8.8 clone ...
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bioRxiv - Immunology 2021Quote: ... The depleted cell fraction was then stained for 20 min at 4 °C with a cocktail of fluorophore-labeled antibodies CD45-PerCPCy5.5 (1:50) (Biolegend), Ly51-PE (1:800 ...
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bioRxiv - Neuroscience 2021Quote: ... the membranes were incubated overnight at 4°C with anti-Aβ primary antibody (6E10 Biolegend, SIG-39320, 1:1000) following incubation with goat anti-mouse secondary antibody (Thermo Scientific ...
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bioRxiv - Genomics 2019Quote: ... The resuspended cells were incubated for 20 min at 4 °C with the fluorophore-labeled antibodies CD45-PerCPCy5.5 (1:50) (Biolegend), Ly51-PE (1:800 ...
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bioRxiv - Immunology 2021Quote: ... Cells were incubated at 4°C for 15 minutes with an Fc receptor blocker (BioLegend TruStain FcX, cat # 422302) according to manufacturer instructions before cocktails of surface antibodies were added for 30 minutes at 4°C ...
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bioRxiv - Immunology 2021Quote: ... Samples were next stained for surface markers for 20min at 4°C with: PerCP-Cy5.5-conjugated anti-TCRβ (H57–597, BioLegend), APC-CY7-conjugated anti-B220 (RA3-6B2 ...
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bioRxiv - Neuroscience 2019Quote: ... Cells were incubated overnight at 4°C with mouse anti-HA primary antibody (1:1000, Anti-HA.11, Biolegend), washed 3× in PBS and incubated with Alexa-conjugated anti-mouse secondary antibody (1:500 ...
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bioRxiv - Immunology 2019Quote: ... in the presence of 1 μl BV421 anti-CD107a mAb for 2 hr at 37°C (H4A3, Biolegend 328626). Streptavidin M280 Dynabeads were conjugated to biotinylated HLA-C*05:01 refolded with P2-AV or P2-AW as described below.
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bioRxiv - Neuroscience 2019Quote: ... followed by overnight incubation at 4°C with primary antibodies diluted in 1% NDS-PBS-Tx (1:1,000 for mouse anti-HA, #901514 BioLegend; 1:1,000 for rabbit anti-TOM20 ...
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bioRxiv - Cell Biology 2021Quote: ... were incubated with Calbryte 630TM (2μM; Stratech) for 30 minutes at 37°C followed by staining with CD42b-BV421 (1:100; Biolegend) for 15 minutes at room temperature and supplementation with 2mM CaCl2 ...
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bioRxiv - Cell Biology 2020Quote: After coating with poly-L-Lysine, fluorodishes were coated overnight at 4 °C with αCD3ε (OKT3, #16-0037-85, eBioscience) + αCD28 (CD28.2, #302914 Biolegend), washed three times and pre-warmed at 37 °C for 10–15 min ...
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bioRxiv - Cancer Biology 2021Quote: ... then resuspended in FACS buffer containing 2 ug/ml APC/Cy7 anti-human HLA-A,B,C (BioLegend, #311425). Cells were incubated with the antibody on ice for 20 minutes in the dark ...
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bioRxiv - Immunology 2022Quote: ... and stained for 30 min at 4°C with a fluorescein isothiocyanate (FITC)-conjugated anti-mouse CD4 Ab (BioLegend). The cells were then fixed ...
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bioRxiv - Neuroscience 2022Quote: ... and incubated at 4°C overnight with the following primary antibodies: mouse anti-C3 (1:200 dilution, 846302, Biolegend), rabbit anti-CD31 (1:200 dilution ...
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bioRxiv - Cell Biology 2022Quote: ... iHeps were fixed in 1.6% paraformaldehyde for 20 min at 37°C and then permeabilized in saponin buffer (BioLegend). Cells were first probed using AAT (Santa Cruz Biotechnologies) ...
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bioRxiv - Molecular Biology 2020Quote: ... 600 μL of WCE was incubated overnight at 4°C with 10μg of monoclonal RNA Poll antibody (Biolegend, USA), 2μg of anti-myc antibody (Sigma) ...
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bioRxiv - Genetics 2022Quote: ... two-week C57BL/6 c-Kit+ bone marrow cells were cultured in media with recombinant mouse interferon alpha (Biolegend) as indicated with media changes every 2-3 days ...
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bioRxiv - Cancer Biology 2022Quote: ... and lineage-negative cells (Lin-) were analyzed for c-Kit (clone 2B8; eBioscience) and Sca-1 (clone D7; BioLegend) expression to discriminate Kit+ Sca-1- (KL ...
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bioRxiv - Microbiology 2019Quote: ... SERINC5 levels were assessed via C-terminal HA tag using the mouse monoclonal HA.11 (Biolegend, San Diego, CA) at 1 μg/ml in Odyssey blocking buffer (LI-COR Biotechnology ...
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bioRxiv - Cancer Biology 2021Quote: Single cell suspensions isolated from treated tumors are individually barcoded with hashtag antibodies (Anti-mouse TotalSeq-C antibodies, Biolegend). Each sample was washed 4 times with PBS + 2% BSA before pooling ...
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bioRxiv - Cell Biology 2021Quote: ... Unfractionated or c-Kit-enriched BM cells were then incubated with purified rat anti-mouse lineage antibodies (CD3, BioLegend, 100202 ...
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bioRxiv - Immunology 2020Quote: ... stained for 20 min at 4 °C in PBS/2% FCS in presence of Fc receptor blocking reagent (Biolegend) with anti-murine CD326-BV605 ...
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bioRxiv - Immunology 2020Quote: ... Sections were stained at 4°C on an agitation stage overnight with anti-CD169 and anti-Ly6G antibodies (BioLegend). Stained sections were microscopically analyzed using Leica SP8 confocal microscope (Leica Microsystem ...
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bioRxiv - Immunology 2020Quote: ... Bacteria were opsonised in heat-inactivated human serum for 30 minutes at 37°C and labelled via incubation with 10μM carboxyfluorescein succinimidyl ester (CFSE) (Biolegend) for 30 minutes at 37°C ...
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bioRxiv - Immunology 2022Quote: ... for 30mins at 37°C, washed, resuspended in FACS buffer (PBS, 2% FBS) and co-stained with anti-CD4 (GK1.5, Biolegend), anti-CD62L (MEL-14 ...