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Citations for BioLegend :
451 - 500 of 819 citations for Borrelia burgdorferi C p Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Molecular Biology 2020Quote: ... MNCs were harvested by ficoll gradient centrifugation and human hematopoietic cells were identified by flow cytometry using the following antibody cocktail: HLA-A/B/C FITC (clone W6/32, Biolegend), mouse CD45.1 PE-CY7 (clone A20 ...
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bioRxiv - Immunology 2021Quote: ... Cell-free BAL fluid was stored at 4°C and processed within 24hr to measure cytokine/chemokine content using a LEGENDplex bead array kit (BioLegend).
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bioRxiv - Immunology 2020Quote: ... 2 × 105 WT or CD56-KO NK92 and YTS cell lines were added to 12-well flat-bottomed plates that were pre-coated overnight at 4°C with anti-NKp30 (clone P30-15, Biolegend) and -CD18 (clone IB4 ...
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bioRxiv - Immunology 2020Quote: 96-well flat-bottomed plates were pre-coated overnight at 4°C with 5 µg/ml anti-NKp30 (clone P30-15, Biolegend) and -CD18 (clone IB4 ...
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bioRxiv - Neuroscience 2021Quote: ... and finally incubated overnight at 4°C with primary antibodies against NKCC1 (T4, 1:1000, DSHB) and tubulin (anti-Tuj1, 1:5000, Biolegend) diluted in the blocking buffer with 0.1% tween ...
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bioRxiv - Cell Biology 2021Quote: ... for 10 min at 4°C and stained with fluorophore conjugated antibodies for 20 min at 4°C (anti-Pdpn PE-Cy7, Biolegend 127411; anti-Thy1 A647, Biolegend 105318 ...
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bioRxiv - Cell Biology 2021Quote: ... for 10 min at 4°C and stained with fluorophore conjugated antibodies for 20 min at 4°C (anti-Pdpn PE-Cy7, Biolegend 127411; anti-Thy1 A647, Biolegend 105318; anti-CD31 APC/Fire 750, Biolegend 102433 ...
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bioRxiv - Microbiology 2021Quote: ... and cells were stained for 30 min at 4°C with a combination of the following anti mouse immunophenotyping antibodies: from Biolegend: PacificBlue-conjugated anti-Ly-6C (clone HK1.4 ...
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bioRxiv - Immunology 2019Quote: Macrophages were stained for 40 min at 4°C with fluorophore-conjugated mAb FITC-anti-CD36 (clone 5-271, Biolegend), or PE-anti-CD209 (clone 120507 ...
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bioRxiv - Microbiology 2021Quote: T cells were washed and incubated with antibodies (described above) at 4°C for surface staining and cell viability dye Zombie UV (Biolegend). Primary antibodies were detected with anti-human IgG secondary antibody ...
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bioRxiv - Bioengineering 2020Quote: ... The following antibodies and dyes were used: anti-human HLA-A,B,C Pacific Blue (clone W6/32, BioLegend®), anti-HA Alexa Fluor 647 (clone HA.11 ...
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bioRxiv - Immunology 2022Quote: ... and then cell surface staining was performed for 30 min at 4°C with fluorochrome-conjugated monoclonal antibodies to mouse antigens purchased from Biolegend: XCR1 ...
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bioRxiv - Immunology 2022Quote: ... BMDCs were pre-incubated for 20 minutes at 37 °C with anti-mouse IFNAR1 (clone MAR1-5A3, 10 μg/mL, Biolegend), anti-mouse IL-12p40 or anti-mouse IL-12p70 Abs or an isotype control before stimulation.
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bioRxiv - Immunology 2019Quote: ... and incubated for 1 h at 4°C in the dark: CD3 (anti-CD3-APC-780; clone 145-2C11, Biolegend), CD4 (anti-CD4-PE-Cy7 ...
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bioRxiv - Neuroscience 2019Quote: ... followed by overnight incubation at 4°C with either anti-beta amyloid 6E10 (mouse, 1:1,000 dilution of 1mg/mL stock) (Biolegend, USA), anti-tau MC1 (mouse ...
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bioRxiv - Immunology 2019Quote: ... to block non-specific binding of antibodies to Fc Receptors and then incubated at 4°C for 30 minutes with Fixable Viability Staining e780 and fluorochrome/biotin conjugated antibodies (purchased from BD Bioscience, Biolegend, Thermofisher Scientific or Miltenyi biotec ...
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bioRxiv - Immunology 2021Quote: ... Cells were harvested 72 hours post transduction and HLA-C expression determined by staining with anti-HLA (W6/32) (Biolegend) antibody ...
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bioRxiv - Genomics 2021Quote: ... Sample from each donor is also stained with unique hash-tag barcoded antibody (Biolegend totalseq-C, cat# 394661, cat# 39466310). After two washes ...
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bioRxiv - Bioengineering 2020Quote: ... cells were recovered from wells and were incubated at 4°C for 30 minutes with antiCD3 antibody (Biolegend, CA, USA) and then were examined by flow cytometry.
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bioRxiv - Immunology 2021Quote: ... Cells were then incubated with bead/Ab mixture for 1 hr at 37°C and then stained with PacificBlue-conjugated anti-CD66b (Biolegend) in PBS ...
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bioRxiv - Immunology 2021Quote: Cell samples went through a series of centrifugations (400g, 5 minutes, at 4°C) and were stained with PE anti-CD45 (30-F11, BioLegend) in staining buffer (see flow cytometry section ...
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bioRxiv - Immunology 2021Quote: ... as determined by 2% agarose gel electrophoresis.49 The soluble chromatin fraction was incubated with either anti-c-Rel antibody or anti-IgG control antibody (Biolegend) coupled to Protein G beads (Cell Signaling ...
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bioRxiv - Immunology 2020Quote: ... Slides were incubated with monoclonal antibodies overnight at 4°C using anti-CD3 (100202, clone 17A2, BioLegend, San Diego, CA) to detect T-cells ...
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bioRxiv - Immunology 2021Quote: ... followed by a 30 minute incubation with the TotalSeq-C antibody pool and 5 μl of a PE/Cyanine7 anti-human CD8 antibody (BioLegend) at 4°C ...
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bioRxiv - Immunology 2022Quote: ... samples were thawed from −80°C and were stained for surface markers: Zombie UV fixable viability dye (Biolegend, Cat#423107), anti-CD3-BUV395 (BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... cells were cultured for 4 h at 37°C in B cell medium containing 1x Cell Activation Cocktail (without Brefeldin A) (BioLegend) and 3h with 1x Protein Transporter Inhibitor Cocktail (ThermoFisher) ...
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bioRxiv - Cell Biology 2022Quote: ... or P14 bone marrow cells from 2-4 mice per genotype were c-kit selected and stained with tagged Total-Seq B antibodies (Biolegend) to CD150 ...
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bioRxiv - Immunology 2022Quote: ... Intracellular cytokine staining was performed in 1X perm buffer for 20-30 min at 4°C using the following conjugated antibodies all purchased from BioLegend: mouse anti-IFN-γ (clone XMG1.2) ...
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bioRxiv - Immunology 2022Quote: ... mice were inoculated with CEA10-mRFP FLARE conidia and lung cells were incubated with 0.05 mg/ml of pHrodo-Zymosan at 37 °C for 2 hours and stained with Survival dye (efluor780, eBioScience) and Ly6G (Pb, BioLegend) or Ly6G (Pb ...
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bioRxiv - Immunology 2022Quote: ... CD4+ T-cells were cultured alone or co-cultured with either of the B-cell populations at a ratio of 3:1 for 60 h at 37°C and 5% CO2 on anti-CD3 (2 μg/mL) (ULTRA-LEAF Biolegend) coated flat bottomed 384 CellCarrier Ultra microplates (PerkinElmer ...
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bioRxiv - Immunology 2022Quote: ... and 30 minutes at 4°C with the following surface antibodies: CD8 PerCP-Cy5.5 (clone 53–6.7, Biolegend, cat#100734), CD44 BV650 (clone IM7 ...
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bioRxiv - Immunology 2022Quote: ... Samples sorted for 10x Genomics single-cell RNAseq were individually labelled with an oligonucleotide-linked hashing antibody (Totalseq-C, Biolegend) in addition to the staining cocktail and sorted into a single tube according to the gating strategy shown in Fig ...
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bioRxiv - Neuroscience 2022Quote: ... Sections were then incubated overnight at 4°C with primary antibodies diluted in blocking solution: anti-βIII Tubulin (1/500, Biolegend), anti-RSK2 (1/100 ...
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bioRxiv - Immunology 2022Quote: ... Samples were then stained for extracellular markers in the presence of Fc block for 30 minutes at 4°C protected from light using the following antibodies: CD3 (APC/Cy7; 1:300; clone 17A2; BioLegend), CD4 (AF488 ...
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bioRxiv - Neuroscience 2022Quote: ... according to manufactures protocol and blocked in 5% BSA/TBS for 1 h and incubated overnight at 4 °C with the following antibodies: mouse anti-CD9 (1:1000; Biolegend), mouse anti-CD63 (1:1000 ...
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bioRxiv - Cancer Biology 2022Quote: ... and supernatants used for HLA-I IP in which purified anti-human HLA-A,B,C antibody (W6/32, BioLegend) was crosslinked to Protein G Dynabeads (Thermo Scientific ...
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bioRxiv - Cell Biology 2022Quote: ... were performed after confirming full differentiation into mature MCs using flow cytometric analysis of lineage-specific c-KIT (rat anti-CD117 Brilliant Violet 421-conjugated, 1:200, Biolegend) and FcεRI (armenian hamster anti-FcεRIα Alexa Fluor 647-conjugated ...
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bioRxiv - Cell Biology 2022Quote: ... Full differentiation into mature MCs was confirmed by expression of lineage-specific c-KIT (rat anti-CD117 Brilliant Violet 421- conjugated, 1:200, Biolegend) and FcεRI (armenian hamster anti-FcεRIα Alexa Fluor 647- conjugated ...
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bioRxiv - Immunology 2023Quote: ... Murine cells were stained for 30 min at 4°C with the following antibodies (all from Biolegend, unless otherwise stated): CD3 APC(Clone:17A2 Cat:100236 ...
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bioRxiv - Systems Biology 2023Quote: ... Cells were precooled for 20 min at 4 °C and then incubated with HDL-SOG for 40 min at 4 °C with 50 μg HDL per ml PBS or with anti-SCRB1 antibody-SOG (BioLegend, 363201 ...
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bioRxiv - Immunology 2023Quote: ... cells were stained in PBS containing 2% FCS at 4°C with the following antibodies: CXCR1-PerCP (1:50; 8F1/CXCR1, BioLegend), CD11b-PE (1:100 ...
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bioRxiv - Immunology 2023Quote: ... cells were stained in PBS containing 2% FCS at 4°C with the following antibodies: CXCR1-FITC (1:100; 8F1/CXCR1, BioLegend), CD19-PerCP (1:50 ...
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bioRxiv - Immunology 2023Quote: ... cells were stained in PBS containing 2% FCS at 4°C with the following antibodies: CD235a-PECy7 (1:20; HI264, BioLegend), CD31-APCCy7 (1:20 ...
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bioRxiv - Microbiology 2023Quote: ... for 30 minutes at 37°C and then incubated with 10 µg/ml of αASC TMS-1 (clone HASC-71, Biolegend) in blocking buffer ...
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bioRxiv - Immunology 2023Quote: ... cells were stained for sorting in PBS containing 2% FCS at 4°C with the following antibodies: CD138-FITC (1:20; MI15, BioLegend), CD45-PerCP-Cy5.5 (1:20 ...
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bioRxiv - Microbiology 2023Quote: ... cells were washed in cold PBS/1% (w/v) BSA and stained for 1 hour at 4°C with AlexaFluor647-conjugated anti-BST2 (Biolegend) or control isotype (Biolegend) ...
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bioRxiv - Microbiology 2023Quote: ... for 5 minutes at 4°C and then fixed using commercial fixation buffer for 3 minutes (ref no. 420801, BioLegend). For intracellular staining of Granzyme B and Perforin ...
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bioRxiv - Microbiology 2023Quote: ... single cell suspensions are incubated with 1X Brefeldin-A for 4 hours at 37°C to block secretion of cytokines (ref no.420601, BioLegend). For all ...
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bioRxiv - Immunology 2023Quote: ... 100 uL/well) were restimulated with coated anti-CD3 antibody (1 ug/mL, 100 uL/well, 4 °C overnight, Clone: OKT3, Biolegend) and soluble anti-CD28 antibody (2 ug/mL ...
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bioRxiv - Cell Biology 2023Quote: ... Red blood cells were lysed by incubation with Red Blood Cell Lysis Buffer containing ammonium chloride for 15 minutes at 4°C (BioLegend). CD34+ selection was performed using AutoMACS cell separation technology following incubation with CD34+ selection beads (Miltenyi Biotech ...