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Citations for BioLegend :
3651 - 3700 of 3777 citations for Recombinant Human KIT His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Cytokine concentration in these samples were determined using CBA mouse Inflammation Kit (BDTM) or ELISA according to the manufacturers’ instructions (Biolegend).
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bioRxiv - Immunology 2024Quote: To differentiate between live and dead cells were firstly stained with the Zombie NIR™ Fixable Viability Kit (BioLegend, 423106), the Zombie Yellow™ Fixable Viability Kit (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... plasma and tumor lysates were performed by flow cytometry using a multiplex bead-based system (LEGENDPlex Mouse Th Cytokine Panel and Mouse Inflammation Panel kits, Biolegend) that allowed the simultaneous determination of the concentration of several cytokines ...
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bioRxiv - Immunology 2023Quote: ... Nucleocapsid level on exosomes was measured by ELISA using precoated ELISA plates (Legend Max SARS-CoV2 Nucleocapsid protein ELISA Kit, 448007, BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... were performed as we described previously48 and according to the Mission Bio protocol using the custom-designed 37-gene myeloid panel kit and 48 oligo-conjugated antibodies against all major BM cell types (Biolegend). Briefly ...
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bioRxiv - Immunology 2023Quote: CD4+ and CD8+ T cells were isolated from the spleen lymphocytes of healthy mice using MojoSort mouse CD4 nanobeads and a MojoSort mouse CD8α selection kit (BioLegend), according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2023Quote: ... were isolated from single cell suspension of lymph nodes and spleen by negative selection using the MojoSort Mouse CD8 T cell isolation kit (BioLegend). Alternatively ...
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Mice generated with induced pluripotent stem cells derived from mucosal-associated invariant T cellsbioRxiv - Immunology 2023Quote: ... CD3+ T cells were purified from C57BL/6 spleen cells with MojoSort mouse CD3 T cell isolation kit (Biolegend, USA), while CD8+T cells were isolated by adding biotin-labeled anti-CD4 antibody into MojoSort mouse CD3 T cell isolation kit ...
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bioRxiv - Cancer Biology 2022Quote: ... the percentage of apoptotic cells was measured using Annexin V-fluorescein isothiocyanate (FITC)/PI kit using FACSCalibur™ Flow Cytometer (Becton Dickinson Biosciences) in accordance with the manufacturer’s instructions (Biolegend).
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bioRxiv - Cancer Biology 2023Quote: ... FITC Annexin V/Propidium Iodide (PI) staining was performed by FITC Annexin V Apoptosis Detection Kit with PI (BioLegend, 640914). Flow cytometry was performed on BD LSR Fortessa (BD Biosciences ...
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bioRxiv - Biochemistry 2023Quote: ... was added 1 day after serum deprivation and cell viability was assessed the following day by using Zombie Fixable Viability Kit (Biolegend) according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2023Quote: ... CD45.1+ GFP+ OT-II CD4+ T cells were purified from mouse spleens using magnetic beads (CD4 T cell MojoSort isolation kit, Biolegend) and activated with 10μg/ml recombinant mouse IL-2 IS (Miltenyi ...
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bioRxiv - Bioengineering 2023Quote: ... Mo were then isolated from the suspension of differentiated BMC using a MojoSort™ Mouse Monocyte Isolation Kit (BioLegend #480154). The expression of Mo surface markers was measured by flow cytometric analysis using fluorescently labeled antibodies against CD11b (BioLegend #101207) ...
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bioRxiv - Cancer Biology 2023Quote: Early and late apoptosis in AMO1 shIRE1 cl.1 and shXBP1 cl.1 cells previously treated with Dox was determined with the FITC Annexin V apoptosis detection kit with propidium iodide (PI) (BioLegend). Briefly ...
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bioRxiv - Immunology 2023Quote: ... CD8+ T cells were enriched from the spleen of OT- I mice using the MojosortTM Mouse CD8+ T cell isolation kit (Biolegend) and stained with 5µM CellTrace Violet (Thermo Fisher ...
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bioRxiv - Immunology 2023Quote: ... neutrophils were isolated by negative sorting by magnetic selection using the Mojosort neutrophil isolation kit according to the suggested protocol (Cat# 480058-BioLegend). Purity was checked by flow cytometry using CD11b and Ly6G surface staining ...
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bioRxiv - Cancer Biology 2023Quote: ... Viability of BMNC (0.25 x106) was assessed by Annexin V and 7-AAD staining (APC Annexin V Apoptosis Detection Kit 7-AAD, Biolegend) using FCM.
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bioRxiv - Physiology 2023Quote: ... Serum samples were prepared for Legendplex assays by diluting 1:50000 for the detection of immunoglobulin isotypes using the mouse immunoglobulin isotyping kit (BioLegend). The assay was performed in a V-bottom plate and data was acquired on a BD Fortessa X-30 cytometer (BD Biosciences) ...
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bioRxiv - Genetics 2023Quote: ... Lin-Sca-1+ c-Kit+ Flt3-CD150+ CD48-on a FACSAria into StemSpan SFEM II media with SCF (100 ng/ml, BioLegend), TPO (50 ng/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed twice using ice-cold PBS and stained using Zombie Aqua™ Fixable Viability Kit (Cat. 423101, Biolegend). Cells were washed and resuspended in FACS buffer (1% BSA ...
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bioRxiv - Cancer Biology 2024Quote: ... T cells were isolated from the spleen and lymph nodes of FVB/N mice using the MojoSort Mouse CD8 T cell isolation kit (BioLegend) as per the manufacturer’s protocol for negative selection strategy ...
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bioRxiv - Microbiology 2024Quote: Cytokine concentrations in supernatants from cultured BMDMΦ or sera of animals were assessed using the Legendplex mouse inflammation kit (BioLegend) following the manufacturer’s instructions as described previously (18 ...
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bioRxiv - Immunology 2024Quote: ... Apoptosis analysis was done with the APC Annexin V (1:50) apoptosis detection kit with PI (1:200) in Annexin V Binding Buffer (Biolegend).
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bioRxiv - Molecular Biology 2020Quote: ... and LCL A382T TDP-43 mutated patients (LCL-TDP382) were incubated with a vitality dye for 15 minutes (Zombie Violet™ Fixable Viability Kit, BioLegend). Then ...
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bioRxiv - Molecular Biology 2020Quote: ... together with the trypsinized cells were harvested into tubes and samples were prepared on ice for flow cytometry using the Pacific Blue Annexin V Apoptosis Detection Kit with 7-AAD (Biolegend: 640926) according to the manufacturer’s protocol ...
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bioRxiv - Molecular Biology 2020Quote: Freshly isolated CD3+ T cells were labeled using CFSE working solution (5 μM) using the CFSE Division Tracker Kit (Biolegend, USA). T cells were incubated for 20 min at 37 ◻ and quenched staining with culture medium ...
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bioRxiv - Immunology 2021Quote: Naïve CD8+ T cells (OT-1 or Pmel-1) were isolated from pooled spleen and lymph nodes using MojoSort™ mouse CD8 T cell isolation kit (Biolegend) following manufacturer’s instruction ...
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bioRxiv - Immunology 2019Quote: ... total CD4+ T cells were first isolated from Foxp3Thy1.1 reporter mice(Liston et al., 2008) by using mouse CD4 T cell isolation kit (Biolegend, catalog 48006), and stained with anti-Thy1.1-PE antibody (eBioscience ...
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bioRxiv - Evolutionary Biology 2021Quote: ... Lcn-2 levels were determined in fecal supernatants using a LEGEND MAX™ Mouse NGAL (Lipocalin-2) ELISA Kit (BioLegend #443708).
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bioRxiv - Cancer Biology 2020Quote: ... fixed and stained for intracellular Ki67 following BD Pharmingen’s procedure for FITC BrdU Flow Kit (Cat# 559619) with two modifications: DNase treatment step was omitted and Ki67-AlexaFluor488 (Biolegend #350507) was used instead of anti-BrdU FITC.
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bioRxiv - Pharmacology and Toxicology 2022Quote: ... The culture supernatants of stimulated cells were collected for cytokine (i.e., IL-4 and IFN-γ) assays using ELISA kits (BioLegend, CA).
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bioRxiv - Microbiology 2022Quote: Lung tissue leukocyte samples were subjected to viability staining and blocking of Fc-receptors using LIVE/DEAD™ Fixable Blue Dead cell stain kit (life technologies) and an anti-CD16/32 antibody (clone 93, BioLegend). Cells were then washed and incubated with a staining mix containing antibodies against the following murine antigens ...
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bioRxiv - Immunology 2022Quote: ... and then stained at room temperature for 15 min with a 1:200 dilution of the LIVE/DEAD Zombie Aqua™ Fixable Viability Kit (Biolegend) reagent to exclude dead cells ...
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bioRxiv - Microbiology 2020Quote: Levels of cytokines and soluble mediators present in plasma of infected mice were quantified using LEGENDplex™ Mouse Inflammatory Panel kit (Biolegend) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... DCs were isolated from the resulted mononuclear cells using a MojoSort™ Mouse Pan Dendritic Cell Isolation Kit (BioLegend cat. 480097). Single molecule FISH experiments were carried out using an RNAscope® Multiplex Fluorescent Assay v2 along with an RNAscope® Probe-Mm-Krt14-C2 (ACD cat ...
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bioRxiv - Immunology 2020Quote: Active TGF-β1 serum levels in patients and healthy donors were measured using LEGEND MAX™ Free Active TGF-β1 ELISA Kit with Pre-coated Plates (Biolegend). The assay was run according to the manufacturer’s recommendations.
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bioRxiv - Immunology 2022Quote: ... cells were washed twice with PBS buffer and stained with a kit of Zombie Aqua™ Fixable Viability (Biolegend, cat.423101) for 20 min at RT ...
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bioRxiv - Cancer Biology 2022Quote: ... the cells were stained with Annexin V/PI using the Apoptosis Detection Kit according to the manufacturer’s protocol (BioLegend, Cat# 640945). Cells were acquired on a BD LSR Fortessa ...
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bioRxiv - Immunology 2022Quote: ... True-Nuclear™ One Step Staining Mouse Treg Flow™ Kit (FOXP3 (clone MF-14) with CD25 and CD4) was used with FOXP3 Fix/Perm Buffer from BioLegend per manufacturer’s protocol ...
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bioRxiv - Immunology 2022Quote: ... Primary antibodies, anti-PDGFRa (AB Online, # ABIN726620), anti-Kitl (R&D, #AB- 455-NA) and anti-c-Kit (Biolegend, clone 2B8) were then stained at a 1:200 dilution for 72hrs ...
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bioRxiv - Microbiology 2022Quote: The amount of TNFα secreted in the supernatants from cell culture and cell lysate was determined by ELISA MAX™ Deluxe Set Mouse TNFα kit (BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Pharmacology and Toxicology 2022Quote: OVA-specific IgE levels were measured using a LEGEND MAX™ Mouse OVA Specific IgE ELISA Kit (BioLegend, San Diego, CA). The plate was washed 4 times with wash buffer followed by addition of Matrix A to standard wells and Assay Buffer to sample wells ...
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bioRxiv - Cancer Biology 2022Quote: Oligo-labeled antibody staining was done on both melanoblasts and melanom cells using the TotalSeq™-A Antibodies and Cell Hashing with 10× Single Cell 3′ Reagent Kit (Biolegend). The HashTag Oligonucleotidess used for the analysis are mHTO01 ...
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Autocrine Sfrp1 inhibits lung fibroblast invasion during transition to injury induced myofibroblastsbioRxiv - Cell Biology 2022Quote: ... an aliquot of 3×105 cells in 1ml MACS buffer was incubated at RT for 15 minutes with 1:500 dilution of Zombie UV™ Fixable Viability Kit (Biolegend). Cells were stained with 1 µl of anti-GFP antibody (Santa Cruz ...
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bioRxiv - Immunology 2024Quote: Cells were stained with 20 µL of surface antibody cocktail with Zombie NIR fixable viability kit (1:400-1000, Biolegend, 423105) in PBS for 20 minutes on ice ...
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bioRxiv - Immunology 2023Quote: ... CD4 T cells were isolated from the spleen and LNs of indicated naïve OT-II strains with Mouse CD4 T Cell Isolation Kit (STEMCELL Technologies 19852 or BioLegend 480033) and labeled with 1.0 µM CFSE (Thermo Fisher ...
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bioRxiv - Cancer Biology 2024Quote: Apoptotic and necrotic cell analysis was performed using Pacific Blue™ Annexin V Apoptosis Detection Kit with 7-AAD (BioLegend, #640926) as per manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... CORSET8 T cells were purified from the TCR Tg donor mice with the MojoSort® Mouse CD8 T Cell Isolation Kit (Biolegend) and labelled with Cell Proliferation Dye eFluor 450 (0.01 mM ...
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bioRxiv - Immunology 2024Quote: ... Lymph node cells were harvested from TCR Tg donor mice and CD8+ T cells were purified with MojoSort® Mouse CD8 T Cell Isolation Kit (Biolegend) and labelled with Cell Proliferation Dye eFluor 450 (0.01 mM ...
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bioRxiv - Neuroscience 2023Quote: ... libraries were prepared according to the Chromium Single Cell 3ʹ with Feature Barcoding technology for Cell Surface Protein Reagent Kit v3 protocol (10X Genomics) with modifications for incorporation of TotalSeq™-A HTO antibodies (clones M1/42; 30-F11, BioLegend). Briefly ...