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Citations for BioLegend :
3601 - 3650 of 3777 citations for Recombinant Human KIT His tagged since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Synthetic Biology 2021Quote: ... cells were stained for surface markers and then prepared for intracellular staining using True-Nuclear Transcription Factor staining kits (Biolegend). For experiments demonstrating stimulation response ...
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bioRxiv - Cancer Biology 2021Quote: NK cells were isolated from C57BL/6 mice (male and female) with the MojoSort mouse NK cell isolation kit (BioLegend) (purity >85 % based on the flow cytometric analysis with NK1.1 and CD49b antibodies) ...
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bioRxiv - Immunology 2021Quote: ... and CD4+ CD25- and CD8+ T cells were purified using the MojoSort Mouse CD4 (supplemented with biotinylated anti-CD25) and CD8 T Cell Isolation Kits (BioLegend), respectively ...
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bioRxiv - Genetics 2021Quote: ... we negatively isolated memory T cells with a modified Pan T cell magnetic-activated cell sorting (MACSR) kit with anti-CD45RA biotin and followed an optimized version of the CITE-seq protocol with TotalSeqTM-A (BioLegend) oligonucleotide-labeled antibodies for a panel of 31 surface proteins (43) ...
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bioRxiv - Immunology 2020Quote: ... Intracellular staining for Foxp3 was performed on single cell suspensions from spleen and LNs specifically using a FOXP3 Fix/Perm Kit (BioLegend).
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bioRxiv - Microbiology 2022Quote: IL-1 cytokine concentrations in the sera of mice or supernatants from cultured BMDMΦ were assessed using the Legendplex mouse inflammation kit (BioLegend) following the manufacturer’s instructions as described previously (42).
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bioRxiv - Molecular Biology 2020Quote: ... and c-Kit on mast cells were analyzed by Flow cytometry using fluorochrome labelled antibodies (PE-CY7–conjugated anti-FcεRIα, MAR-1 Biolegend #134317; allophycocyanin-CY7–conjugated anti–c-Kit, 2B8, Biolegend, #105825). The YFP+ cells were FACS-sorted and deletion of the Gata2 gene was determined by PCR to be greater than 99%.
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bioRxiv - Immunology 2021Quote: ... and donor-derived CD4 T cells were analyzed by flow cytometry following magnetic bead–enrichment with MojoSort Mouse CD4 T Cell Isolation Kit (BioLegend).
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bioRxiv - Immunology 2021Quote: ... CD4 T cells from the spleen and peripheral lymph nodes of C57BL/6 mice on 22-24 dpi with LCMV-c13 were harvested and enriched for CD4 T cells using a MojoSort Mouse CD4 T Cell Isolation Kit (BioLegend) prior to surface staining ...
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bioRxiv - Immunology 2019Quote: ... This pooled sample of ∼300 million cells was then evenly distributed across each of the 372 wells of a LEGENDScreen kit (BioLegend) containing reconstituted PE antibodies (Supplementary Table 2) ...
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bioRxiv - Immunology 2021Quote: ... cells were washed in 1xPBS supplemented with 0.5% bovine serum albumin (BSA) and 2 mM EDTA (FACS buffer) and stained with Zombie Aqua Fixable Viability kit (Biolegend) and incubated for 20 min at 4°C followed by surface staining for 30 min at 4°C ...
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bioRxiv - Immunology 2021Quote: CD3+ T cells from lung single-cell suspensions (1×107 cells) were isolated by negative selection using the MojoSort mouse CD3+ T-cell Isolation kit (Biolegend) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were washed twice using ice-cold PBS and stained using Zombie Aqua(tm) Fixable Viability Kit (Cat. 423101, Biolegend). Cells were washed and resuspended in FACS buffer (1% BSA ...
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bioRxiv - Cancer Biology 2020Quote: ... FOXP3 APC (Clone FJK-16s eBioscience) used after cell permeabilization with the kit Perm Buffer (10X) and Fix/Perm Buffer (4X) (BioLegend), according to manufacturer’s instructions ...
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bioRxiv - Neuroscience 2021Quote: ... Tissue samples were blocked/permeabilized using 10% normal goat serum in 0.3% Triton-X in PBS for 3 hours at room temperature followed by additional avidin biotin blocking using a kit according to manufacturer’s instructions (BioLegend, 927301). Primary immuno-staining was performed using antibodies ...
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bioRxiv - Biochemistry 2022Quote: To monitor the cytotoxicity of MbxA the release of cytosolic LDH was measured as an indicator of membrane damage using the LDH Cytox Assay Kit (Biolegend).
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bioRxiv - Immunology 2022Quote: Naïve CD4+ T cells were enriched from mouse spleen and lymph nodes by negative selection using MojoSort Mouse CD4 Naive T Cell Isolation Kit (MojoSort, Biolegend) following the manufacturer’s instructions ...
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bioRxiv - Physiology 2022Quote: ... Samples were incubated with anti-c-kit rat monoclonal antibody (1:500 v/v, ACK2, BioLegend, San Diego, CA, USA) overnight (4ºC) ...
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bioRxiv - Cancer Biology 2022Quote: ... and analyzed for apoptosis/necrosis using the APC Annexin V Apoptosis Detection Kit with Propidium Iodide (BioLegend, San Diego, CA). Cells were analyzed using a BD Symphony Flow Cytometer (BD Biosciences) ...
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bioRxiv - Immunology 2021Quote: ... Cell-free BAL fluid was stored at 4°C and processed within 24hr to measure cytokine/chemokine content using a LEGENDplex bead array kit (BioLegend).
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bioRxiv - Immunology 2020Quote: ... The cells were stained extracellularly to detect ILC surface markers and intracellularly for IFNγ expression using the intranuclear staining kit (Biolegend). Sorted ILC1-like and NK cells were stimulated with human (h ...
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bioRxiv - Immunology 2022Quote: ... Naïve CD4+ T cells were isolated using the Mojosort Mouse CD4 Naïve T Cell Isolation Kit (Biolegend, San Diego, CA) using LS columns on a QuadroMACS magnet (Miltenyi Biotec ...
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bioRxiv - Immunology 2022Quote: ... LN and thymic homogenates were stained with a cocktail of antibodies against CD4-FITC and CD25-APC (for LN Tregulatory cells; Mouse Regulatory T Cell Staining Kit #1, eBioscience) or CD4-PB (Biolegend), CD8-FITC ...
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bioRxiv - Immunology 2022Quote: ... TCR stimulation was evaluated by measuring IL-2 concentration in culture supernatant using an IL2 ELISA kit (#423001, #423501, #431001 and #421101, BioLegend).
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bioRxiv - Immunology 2022Quote: ... 5×104 Tconv (CD4+CD25-) cells from TFAM control were labeled with CFSE Cell Division Tracker Kit (Cat# 423801, BioLegend) and cultured with mitomycin-treated wild type splenocytes ...
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bioRxiv - Neuroscience 2022Quote: ... CD4+ T cells were isolated from the spleens and draining lymph nodes of 2D2 mice using the CD4+ isolation kit (BioLegend) and co-cultured with irradiated wild-type splenocytes at a ratio of 1:5 ...
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bioRxiv - Cancer Biology 2019Quote: ... approximately 1 * 106 cells from dLN and 5 × 106 tumor cells were first stained with Zombie Red Fixable Viability Kit (BioLegend) for 30’ at room temperature and then incubated on ice for 15’ with an anti-mouse CD16/32 antibody (BioLegend) ...
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bioRxiv - Cancer Biology 2019Quote: ... Cells were either stained with DAPI for viability or fixed with 1% PFA and stained with zombie aqua fixable viability kit (Biolegend). Viable cells were sorted on a FACSAria flow cytometer (Becton Dickinson) ...
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bioRxiv - Genomics 2020Quote: Cell and nuclei labelling were performed according to an adapted Biolegend cell hashing protocol (TotalSeq™-A Antibodies and Cell Hashing with 10x Single Cell 3’ Reagent Kit v3 3.1 Protocol, Biolegend). Briefly ...
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bioRxiv - Immunology 2021Quote: ... Cell viability staining was routinely performed for plate stimulations and U87 co-culture using fixable violet or near-infrared viability dyes (Zombie UV fixable viability kit [Biolegend] ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were washed with PBS and stained with Zombie Aqua fixable live-dead kit as per supplier’s instructions (Biolegend, 423101) for 30 minutes at 4°C ...
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bioRxiv - Immunology 2020Quote: ... Cell viability staining was routinely performed for plate stimulations and U87 co-culture using fixable violet or near-infrared viability dyes (Zombie UV fixable viability kit [Biolegend] ...
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bioRxiv - Immunology 2020Quote: ... The percentage of apoptotic and necrotic cells were then determined by flow cytometry using the FITC Annexin V apoptosis detection kit with PI (BioLegend) following manufacturer’s instructions.
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bioRxiv - Microbiology 2019Quote: ... and either directly fixed for ChIP or stained for dead cells using the Zombi NIR Fixable Viability Kit (Biolegend: #423106) as per the manufacturer’s instructions for subsequent FACS sorting (MHV-68-H2BYFP) ...
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bioRxiv - Cancer Biology 2020Quote: ... washed two times with ice cold PBS and processed for staining using FITC Annexin V Apoptosis Detection Kit with 7AAD (Cat # 640922, BioLegend), according to manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were then labeled according to the manual of the FITC ANNEXIN V Apoptosis Detection Kit with 7-AAD (BioLegend) with a modification where the amount of 7-AAD was doubled.
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bioRxiv - Immunology 2021Quote: ... lymphocytes were stained in 100 μl PBS with a viability dye (Zombie Aqua™ Fixable Viability Kit, Biolegend, the Netherlands) for 15 min at RT in the dark ...
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bioRxiv - Immunology 2021Quote: ... CD8+ T cells were then isolated from the spleens using the MojoSort™ Mouse CD8 T Cell Isolation Kit (BioLegend), following the manufacturer’s guidelines ...
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bioRxiv - Immunology 2022Quote: ... FSC-A/H and dead cell exclusion by using a Fixable Viability Kit (Near-IR staining, dilution 1:500, Biolegend).
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bioRxiv - Immunology 2022Quote: 3×106 cells from each tonsil donor (n=3) at each time point were stained with the Zombie Red fixable viability kit (Biolegend) for 10 min at RT ...
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bioRxiv - Immunology 2022Quote: The presence of IL-17A in T-cell culture supernatants and of IL-6 and IL-8 in fibroblast culture supernatants was quantified by ELISA according to manufacturer instructions (deluxe or standard kits, respectively, all BioLegend). Plates were read at 450 nm using a Spark 10M (Tecan) ...
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bioRxiv - Developmental Biology 2022Quote: ... Intracellular staining for epitopes was achieved using the fixation buffer and intracellular permeabilization buffer kit (BioLegend, San Diego, CA, USA). Antibodies are listed in Table S4 ...
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bioRxiv - Immunology 2022Quote: ... positive selection of the neutrophils was performed by staining the flow through cells with anti-Ly-6G-APC for 20 minutes at 40C followed by isolating the stained cells with anti-APC nanobeads using a mojosort APC nanobead kit (Biolegend). Purity and viability of the cells were checked by flow cytometry using an anti-mouse Gr1 antibody and fixable viability dye ...
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bioRxiv - Immunology 2022Quote: ... The PBMCs were fixed and permeabilized using Foxp3 / Transcription factor kit (eBioscience) and stained intracellularly with anti-Granzyme-B-Alexa-Fluor-700 (Biolegend), anti-TNF-α-efluor-450 (Invitrogen) ...
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bioRxiv - Cell Biology 2022Quote: ... were performed after confirming full differentiation into mature MCs using flow cytometric analysis of lineage-specific c-KIT (rat anti-CD117 Brilliant Violet 421-conjugated, 1:200, Biolegend) and FcεRI (armenian hamster anti-FcεRIα Alexa Fluor 647-conjugated ...
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bioRxiv - Cell Biology 2022Quote: ... Full differentiation into mature MCs was confirmed by expression of lineage-specific c-KIT (rat anti-CD117 Brilliant Violet 421- conjugated, 1:200, Biolegend) and FcεRI (armenian hamster anti-FcεRIα Alexa Fluor 647- conjugated ...
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bioRxiv - Immunology 2024Quote: ... CD4+ T-cells were enriched from the spleen of OT-II mice using the MojoSort mouse CD4+ T-cell isolation kit (BioLegend) and labelled with CellTrace Violet (5 μM ...
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bioRxiv - Microbiology 2024Quote: Levels of cytokines and soluble mediators present in serum of infected mice were quantified using LEGENDplex Mouse Inflammatory Panel kit (BioLegend) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... and the resulting cDNA was amplified using as PCR oligos the partial TruSeq read 1 adaptor (provided in the 10x kit) and an additive primer (a partial TruSeq read 2, sequence provided by Biolegend and synthesized by Integrated DNA Technologies) ...
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bioRxiv - Immunology 2024Quote: ... Naive CD8+ T cells were isolated by negative selection using the MojoSort Mouse CD8+ Naïve T cell Isolation Kit as per manufacturer’s instructions (BioLegend, >90% purity). Live cells were counted by trypan blue dye exclusion and resuspended at a concentration of 106 cells/mL in complete T cell media (RPMI-1640 (Cytiva ...