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Citations for BioLegend :
3501 - 3550 of 5512 citations for Recombinant Human APCS Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: ... Anti-CD69 (H1.2F3, PE/Cy7, B253212, 104512), Anti-CD3 (17A2, APC/Cyanine7, B283940, 100222) antibodies were purchased from BioLegend (Bxxxxxx) or BD Biosciences (xxxxxxx).
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bioRxiv - Immunology 2021Quote: ... Cells were then washed in 1x eBioscience Permeabilization Buffer twice and incubated with indicated intracellular antibodies including anti-TNF-PE (MAb1) and anti-IFN-γ-APC (4S.B3) from BioLegend at room temperature for 1 hour ...
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bioRxiv - Immunology 2021Quote: ... followed by selection of memory B cells by positive expression of CD20 and CD27 (CD20-FITC, clone 2H7; CD27-APC-Cy7, clone O323, both BioLegend) but not immunoglobulin D (IgD-PE ...
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bioRxiv - Immunology 2022Quote: ... prior to incubation with staining antibodies (PE/Cy7 anti-mouse CD45, BV711 anti-mouse CD3, BV421 anti-mouse CD19, FITC anti-mouse CD31, and APC anti-mouse podoplanin, all BioLegend) and one Hashtag antibody per replicate using BioLegend TotalSeq-B03 (catalog numbers 155831 ...
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bioRxiv - Microbiology 2022Quote: ... the BrdU-labeled splenocytes were surface stained for T-cell (CD3e-APC; eBioscience) marker after blocking with anti-mouse CD16/32 antibodies (BioLegend). Cells were then permeabilized and treated with DNase to expose BrdU epitopes followed by anti-BrdU-FITC and 7-AAD (7-amino-actinomycin D ...
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bioRxiv - Pathology 2022Quote: ... The pellet was further washed once with FACS buffer (0.5% BSA/0.5 mM EDTA in PBS) and stained with APC-conjugated anti-mouse CD45 (1:300, BioLegend, CAT#103112), BV510-conjugated anti-mouse CD11b (1:300 ...
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bioRxiv - Immunology 2022Quote: ... Samples were then stained for extracellular markers in the presence of Fc block for 30 minutes at 4°C protected from light using the following antibodies: CD3 (APC/Cy7; 1:300; clone 17A2; BioLegend), CD4 (AF488 ...
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bioRxiv - Genomics 2022Quote: ... eF450 (anti-GL7, eBioscience, 48-5902-82), APC (anti-CD86, eBioscience, 17-0862-82) and PE (anti-CXCR4, BioLegend, 146505). Also ...
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bioRxiv - Immunology 2023Quote: VLP-producing Expi293F cells were extracellularly and intracellularly stained with Allophycocyanin (APC) anti-Flag (DYKDDDDK) tag antibody (1:500) (Biolegend) and intracellularly stained with the Fluorescein isothiocyanate-labeled KC57 (anti-HIV-1 p24 ...
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bioRxiv - Immunology 2023Quote: ... Single cell suspensions were stained as above with efluor780 viability dye prior to incubation at 37C for 10 min with CCR7-APC-Fire810 (clone G043H7, Biolegend) and FcγR block ...
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bioRxiv - Microbiology 2024Quote: ... parasites were washed twice with FACS buffer and stained with (1:100) goat anti-mouse IgG-APC (BioLegend, cat# Poly4053). After incubation ...
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bioRxiv - Immunology 2024Quote: ... Following volumes were added in a single tube containing 100ul FACS buffer per sample: 0.625uL per sample of APC/Cyanine7 anti-mouse CD19 (Biolegend 152412), 1.25uL per sample of Brilliant Violet 421™ anti-mouse CD38 (Biolegend 102732) ...
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bioRxiv - Physiology 2023Quote: ... the resulting single cell suspension was incubated at 37°C for 30 minutes with Integrin β1 antibody (APC anti-mouse/rat CD29; 102216; Biolegend) at 1:100 dilution ...
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bioRxiv - Genomics 2024Quote: ... Antibodies were added to stain surface markers for 30 min at 4°C (CD33 BV711, BD; CD11b APC-Cy7, BD; Zombie Violet, BioLegend). After 30min ...
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bioRxiv - Immunology 2024Quote: ... and a secondary antibody goat anti-mouse antibody conjugated with APC or rat anti-mouse antibody conjugated with Alexa Fluro 700 (BioLegend). These stained cells were fixed with fixation buffer (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... which were obtained from indicated sources: APC anti-mouse I-A/I-E (MHC II) (clone M5/114.15.2, BioLegend, 1:500), FITC anti-mouse I-A/I-E (MHC II ...
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bioRxiv - Immunology 2024Quote: ... were isolated ex vivo from mouse peritoneal cavity by intraperitoneal lavage followed by magnetic bead-based enrichment for c-Kit+ cells using anti-CD117 (c-Kit)-APC antibody (Biolegend) and APC microbeads (Miltenyi Biotec) ...
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bioRxiv - Cell Biology 2023Quote: ... PP and PC populations were identified using anti-E-cadherin-PE and anti-CD73-APC antibody staining (1:150; BioLegend). PP- and PC-specific gates were set after spectral compensation using appropriate fluorescent minus (FMO ...
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bioRxiv - Bioengineering 2024Quote: ... Biotinylated rasburicase was reacted for 20 min on ice with 4:1 molar ratio of biotin to streptavidin-conjugated PE or streptavidin-conjugated APC (Biolegend). Streptavidin-conjugated FITC (BioLegend ...
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bioRxiv - Cancer Biology 2024Quote: ... The dissociated tumor cells were stained with AF488 anti-CD8 antibody and APC anti-CD11c antibody (BioLegend, San Diego, CA) and subjected to flow cytometry analysis to access infiltrated immune cells in the tumor microenvironment ...
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bioRxiv - Bioengineering 2023Quote: ... Biotinylated uricase was reacted for 20 min on ice with 4:1 molar ratio of biotin to streptavidin-conjugated PE or streptavidin-conjugated APC (Biolegend). Streptavidin-conjugated FITC (BioLegend ...
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bioRxiv - Biochemistry 2023Quote: ... Cells were stained for sgRNA expression with an APC conjugated anti-mouse CD90.1/Thy1.1 antibody (no. 202526, BioLegend; 1:400) and Human TruStain FcX Fc receptor blocking solution (no ...
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bioRxiv - Physiology 2023Quote: ... Samples were next stained with surface marker antibodies (CD45-AF700 1:500 103128 Biolegend, CD31-Pacific Blue 1:500 102421 Biolegend, TER119-BUV496 1:500 741079 BD, PDGFRα-APC 1:500 135907 Biolegend, ITGβ4-AF405 1:500 FAB405V Thermofisher ...
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bioRxiv - Immunology 2023Quote: ... electroporated Jurkat cells were interrogated by pMHC-multimer staining with the following surface panel: anti-CD3 APC Fire 50 (SK7, Biolegend), anti-CD8 Pacific Blue™ (RPA-T8 ...
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bioRxiv - Immunology 2023Quote: ... Pooled cells from n = 3 - 5 mice/cohort were stained with PE- or APC-conjugated antibodies (from either BD or BioLegend) against murine F4/80 (clone ...
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bioRxiv - Immunology 2023Quote: ... Pacific Blue (or v450), and APC-Cy7 (or Alexa Fluor-e780)-conjugated antibodies (1:100, CD4, CD8) purchased from Biolegend. After cell surface marker staining ...
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bioRxiv - Microbiology 2023Quote: ... cells were washed and stained by incubation at 4 °C for 30 min with 40 µl of mAb anti-membrane marker mix containing CD4 APC/Cy7 (Biolegend), CD19 FITC ...
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bioRxiv - Immunology 2023Quote: ... Cells were permeabilized for 20 mins at 4°C and then stained overnight with IFN-γ-APC (clone 4S.B3, Biolegend) antibody at 4°C.
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bioRxiv - Immunology 2023Quote: ... anti-CD25(Percp-Cy5.5, #302626), anti-CD69(APC, #310910), anti-mouse CD25(PE, #12-0251-83), anti-mouse CD69(FITC, #104506) from Biolegend, anti-human IgG(SureLight® PE ...
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bioRxiv - Bioengineering 2023Quote: ... the cells were stained for 30 min at 4°C with surface antibodies: anti-mouse CD3 (Biolegend, APC Fire 810), CD4 (Biolegend ...
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bioRxiv - Bioengineering 2023Quote: ... 50 µL aliquots of marrow cells were immunostained with 5 µL Alexa Fluor 488-conjugated rat IgG1 anti-mouse CD41 or APC-Cy7-conjugated rat IgG2b anti-mouse CD117 (BioLegend) and incubated at 4°C for 15 minutes ...
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bioRxiv - Cell Biology 2023Quote: ... Satellite cells were FACS-purified using either Pax7-nGFP reporter or the following combinations of cell surface markers: APC anti-mouse Ly-6A/E (Sca-1) Antibody (108111, BioLegend), mouse Integrin alpha 7 Alexa Fluor 750-conjugated Antibody (FAB3518S ...
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bioRxiv - Cancer Biology 2023Quote: ... and incubated for 30 minutes on ice with the following antibody: anti-CD44 APC/Fire 750 (1:100, Biolegend, 103061), anti-CD24 PE (1:20 ...
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bioRxiv - Cancer Biology 2023Quote: ... Viability of BMNC (0.25 x106) was assessed by Annexin V and 7-AAD staining (APC Annexin V Apoptosis Detection Kit 7-AAD, Biolegend) using FCM.
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bioRxiv - Pathology 2023Quote: ... Ldlr−/− mice fed a chow diet or HC diet for 4 days were anesthetized using Isoflurane and injected intravenously with 5 μg anti-mouse TIM4-APC antibody (RMT4-54, BioLegend). They were then euthanized 5 minutes after injection by cervical dislocation and perfused through the portal vein with PBS and then with 4% ice-cold PFA ...
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bioRxiv - Immunology 2023Quote: ... Single cell lymphocyte suspensions were isolated as described above and stained with anti-CD45-APC (Biolegend 103112, clone 30-F11), anti-CD3e-PE (Biolegend 100308 ...
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bioRxiv - Biochemistry 2023Quote: ... Cell pellets were collected and resuspend by 500 μL (for 80 mL cord blood) Dilution Buffer and then incubated by 10 μL CD34-APC antibody (343510, BioLegend) on ice for 30 min ...
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bioRxiv - Genetics 2023Quote: ... Primary antibody staining was carried out at 4°C overnight with the following antibody dilutions in blocking buffer: CD52-APC (clone HI186, BioLegend) 1:200 ...
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bioRxiv - Bioengineering 2024Quote: ... 100 μL of staining solution containing APC SIINFEKL tetramer (1:600 dilution) and fluorescein isothiocyanate (FITC) CD8 antibody (1:100 dilution, Biolegend) was added to each tube in the ice bath and incubated for 30 minutes in the dark ...
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bioRxiv - Neuroscience 2024Quote: ... This process was repeated once and then cells were resuspended in the antibody solution containing APC anti-rat CD45RA (Cat# 202313, Biolegend) and FITC anti-rat CD3 (Cat# 201403 ...
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bioRxiv - Microbiology 2024Quote: ... Cells present in the suspension were stained on ice for 30 minutes with APC-conjugated anti-Ly-6G (clone 1A8) or PE-conjugated anti-CD63 (Biolegend) and then washed two times in FACS buffer (Biolegend) ...
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bioRxiv - Immunology 2024Quote: ... Sections were saturated with BSA 2% PBS and then incubated overnight with an APC-conjugated anti-CD45 antibody (clone 30F11, Biolegend) at 4°C ...
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bioRxiv - Immunology 2024Quote: ... Apoptosis analysis was done with the APC Annexin V (1:50) apoptosis detection kit with PI (1:200) in Annexin V Binding Buffer (Biolegend).
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bioRxiv - Immunology 2019Quote: ... human T cells were directly enriched from PBMC by negative selection using MojoSort™ Human CD3 T Cell Isolation Kit (Biolegend, CA) following the manufacturer’s instructions ...
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bioRxiv - Microbiology 2020Quote: ... approximately 2-10 million total human lung cells were resuspended in MACS buffer and blocked with Human TruStain FcX (Biolegend cat# 422032) for 15min at 4°C followed by HTII-280 antibody (Terrace Biotech ...
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bioRxiv - Cancer Biology 2019Quote: ... The TDEs were then washed and stained for intracellular cytokines using the PE/Cy7 anti-human IL-10 antibody (clone JES3-9D7) and Brilliant Violet 421™ anti-human LAP (TGF-β1) antibody (TW4-2F8) (BioLegend, CA). TDEs isolated from mice serum were stained with APC anti-human CD63 Antibody (Clone H5C6 ...
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bioRxiv - Bioengineering 2024Quote: ... The levels of cytokines IL-2 and IFN-γ were quantified using ELISA MAX Deluxe Set Human IL-2 and ELISA MAX Deluxe Set Human IFN-γ (BioLegend).
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bioRxiv - Cancer Biology 2021Quote: ... cells were incubated with human Fc receptor block (BioLegend) and stained with cell surface antibodies (Extended Data Table 4 ...
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bioRxiv - Cancer Biology 2021Quote: ... followed by incubation with human Fc receptor block (BioLegend) for 10 min at 4°C ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were incubated with human Fc receptor block (BioLegend) and stained with cell surface antibodies (1:20 anti-CD3-Am Cyan [clone SK7 ...