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Citations for BioLegend :
3401 - 3450 of 5512 citations for Recombinant Human APCS Protein since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... and anti-CD49a APC (1:50; clone HMα1) and anti-CD11b BV570 (1:100; clone M1/70) from BioLegend. Cells were fixed for 30 min at 4°C using eBioscicencesTM FoxP3/Transcription factor staining buffer set (Thermo Fisher ...
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bioRxiv - Immunology 2022Quote: ... cells were incubated with A32-APC (3.3 μg/ml) for 25 minutes and labeled with anti-CD3-AF700 (SK7, Biolegend) and anti-CD32-PE-Cy7 (FUN-2 ...
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bioRxiv - Immunology 2022Quote: ... The suspension of mouse CD4+ T cells and the spinal cord sections were stained with APC-Cy7-conjugated anti-mouse CD4 (clone GK1.5, Biolegend), BV510-conjugated anti-mouse CD4 (clone RM4-5 ...
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bioRxiv - Cell Biology 2022Quote: ... Cells were stained 20 min with APC-conjugated anti-CD24 antibody (1:100 dilution, 138506, Biolegend, San Diego, CA) and FITC-conjugated anti-Epcam antibody (1:500 dilution ...
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bioRxiv - Immunology 2020Quote: ... anti-CD38-APC (90, eBioscience), anti-IgM-PE/Cy7 (II/41, eBioscience) and anti-IgD-PerCP/Cy5.5 (11-26c.2a, Biolegend). The working concentration of the antibodies is 2.5μg/ml.
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bioRxiv - Immunology 2021Quote: ... anti-mouse Ig light chain lambda APC # 407306 and anti-mouse IgG light chain kappa PE both from Biolegend, San Diego ...
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bioRxiv - Immunology 2020Quote: ... or fluorescent probes (Streptavidin-Percp and -APC from BD Pharmingen, Streptavidin-PE from Invitrogen and Streptavidin -BV421 from Biolegend) were used.
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bioRxiv - Cancer Biology 2020Quote: ... Samples were then washed and stained with an APC-conjugated secondary antibody and PE-Cy7-CD45 antibody (103114, Biolegend) and then analyzed by flow cytometry using BD FACS LSR-II (BD Biosciences ...
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bioRxiv - Genomics 2022Quote: CRISPR/Cas9 RNP-treated Th2 cells were stained intracellularly with APC anti-GATA3 antibody (Biolegend #653806, 1:40 dilution) at room temperature for 30 minutes using True-Nuclear Transcription Factor Buffer Set (Biolegend #424401 ...
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bioRxiv - Neuroscience 2019Quote: ... in Alexa Fluor® 488) and rat anti-mouse CD31 (1:100; Biolegend 390 in APC/Fire™ 750) for 30 minutes at 4°C ...
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bioRxiv - Immunology 2020Quote: ... and Tregs (anti-CD3-BV510, anti-CD4-PE, anti-CD8-AF700, anti-CD25-APC (1:100, Biolegend, Cat#302610), anti-FoxP3-AF488 (1:100 ...
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bioRxiv - Immunology 2020Quote: ... Th-17 (anti-CD3-BV510, anti-CD4-PE, anti-CD8-AF700, anti-IL17A-APC (1:100, Biolegend, Cat#512334)) and Tregs (anti-CD3-BV510 ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were collected by scraping and stained with APC-conjugated anti-mouse H-2Kb bound to SIINFEKL (141606, BioLegend), PerCP-conjugated anti-mouse CD86 (105025 ...
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bioRxiv - Cell Biology 2021Quote: ... Then, cells were staining with CD8 (mouse CD8-APC-Cy7, eBioscience, 47-0081-82) and Zombie UV (Biolegend, 427107), followed by flow cytometry analysis.
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bioRxiv - Systems Biology 2021Quote: ... and then stained the cells for 30 min with 1:500 anti-NGFR APC-labeled clone ME20.4 (BioLegend, 345107). We washed the cells five times with 0.1% BSA/PBS and followed with one final wash with PBS for 2 min at room temperature ...
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bioRxiv - Cancer Biology 2021Quote: ... Apoptosis was determined by detecting phosphatidylserine using the APC-Annexin-V Apoptosis Detection Kit with PI (Biolegend, CA, USA). Briefly cells were harvested ...
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bioRxiv - Cancer Biology 2020Quote: ... and naïve 129/SvJae spleens were stained for regulatory T cells with anti-CD127 APC (clone SB/199, Biolegend). Cells were fixed with fix buffer (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... stimulated library was stained for its respective marker with the following antibody: MHCII (APC anti-mouse I-A/I-E Antibody, Clone M5/114.15.2 Biolegend 107613), CD40 (APC anti-mouse CD40 Antibody ...
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bioRxiv - Developmental Biology 2020Quote: ... biotinylated anti-VE-cadherin (clone 11.D4.1) followed by incubation with Streptavidin-APC (all purchased from BD Pharmingen or Biolegend). Anti-mouse VE-cadherin (VC ...
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bioRxiv - Immunology 2022Quote: ... MR1 surface expression was evaluated by flow cytometry with APC-labeled mouse anti-MR1 mAbs 26.5 (Biolegend, Cat#361108) and APC-labeled mouse IgG2a (clone MOPC-173 Biolegend ...
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bioRxiv - Cell Biology 2022Quote: ... 1µg/mL for FC) APC mouse IgG1, k isotype Control (400120, 1µg/mL for FC) were purchased from Biolegend.
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bioRxiv - Cell Biology 2022Quote: ... anti-CD19 (APC, clone ID3), anti-CD45.2 (FITC, clone 104), and anti-TCRβ (PECy7, clone H57-597) from Biolegend, and anti-CD8a (efluor450 ...
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bioRxiv - Microbiology 2022Quote: ... then retro-orbitally injected with 2 μg/mouse of APC anti-mouse CD45 (Biolegend, cat#103112; clone 30-F11) antibody in 50 μL PBS for intravenous labeling of CD45+ circulating lymphocytes 15 minutes prior to euthanization ...
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bioRxiv - Immunology 2022Quote: ... Activation of CAR-MAIT and CAR CD8+ T cells were determined with CD25 staining using CD25-APC antibody (Biolegend). CAR expression was determined with Human Her2 / ErbB2 Protein ...
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bioRxiv - Pathology 2022Quote: ... The following antibodies were used at 1:200 unless specified: anti-CD9 (clone MZ3, FITC, APC/Fire750, biotin; BioLegend), anti-CD31 (clone 390 ...
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bioRxiv - Neuroscience 2023Quote: ... cat no: 130-123-284) and allophycocyanin (APC) conjugated rat anti-mouse CD45 (1:100, Biolegend, cat no: 103111) in blocking solution for 30 minutes on ice in the dark ...
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bioRxiv - Neuroscience 2023Quote: ... For further experiments viable CD45highCD8+ cells were labeled with rat anti-CD45 APC (1:100, catalog no. 103111; BioLegend), rat anti-CD8 PerCP/Cyanine5.5 (1:100 ...
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bioRxiv - Immunology 2022Quote: ... Followed by ACE2-biotin at 1 μg/mL (RnD Systems) incubation for 30 minutes and then SA-APC (Biolegend) incubation for 1 hour ...
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bioRxiv - Physiology 2022Quote: ... Cells were centrifuged at 350xg for 2.5 minutes and re-suspended in a primary antibody cocktail including CD45-APC (BioLegend), F4/80-AF488 (BioLegend) ...
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bioRxiv - Biochemistry 2024Quote: ... and then in a T cell activation analysis cocktail including: anti-mouse CD3 clone 17A2 APC conjugate (BioLegend, 100235), rat anti-mouse CD4 AlexaFluor700 conjugate (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... Intracellular cytokine staining of APC-labeled anti-IFNγ (clone: XMG1.2; eBioscience) and FITC-labeled anti-TNFα (clone: MP6-XT22; BioLegend) were performed using the BD Cytofix/Cytoperm ...
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bioRxiv - Immunology 2024Quote: ... CD33 APC (clone P67.6, catalog #: 366605), LOX-1 PE (clone: 15C4, catalog #: 358603), CD11b APC-Cy7 (clone ICRF44, catalog #: 301341) from BioLegend; CD16 BV421 (clone 3G8 ...
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bioRxiv - Immunology 2023Quote: ... Antibody staining was performed on ice for 1 hour with APC/Cy7 anti-mouse CD45 (clone 30-F11; BioLegend), rat monoclonal FITC anti-mouse Ly6B.2 (clone 7/4 ...
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bioRxiv - Cancer Biology 2023Quote: ... naïve CD4+ T cells were co-cultured with APCs at indicated ratio in the presence of 0.5 μg/mL (anti-CD3 BioLegend), 0.5ng/mL TGFβ (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... or 30 µL 1% FITC-Dextran70 (TdB Labs, 21024) to visualize vasculature and 7 µL APC-Ly6G (Biolegend, 127614) antibody to label neutrophil 30 min before imaging.
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bioRxiv - Genomics 2023Quote: ... Cells were stained on ice in the dark for 30 mins with CD34-APC (clone 581; Biolegend; 1:100), CD38-PE/Cy7 (clone HB7 ...
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bioRxiv - Immunology 2023Quote: ... and stained for 45 min on ice in the dark with an APC anti-IFNγ (1:100 dilution, clone XMG1.2, Biolegend) intracellular marker ...
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bioRxiv - Immunology 2022Quote: ... The c-kit+ fraction was stained with antibodies against CD117 (c-kit APC, clone 2B8, Biolegend, dilution 1/100), Sca-1 (APC-Cy7 ...
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bioRxiv - Bioengineering 2023Quote: ... Successful Differentiation was confirmed by flow cytometry using CD41 (APC, clone HIP8, Biolegend Cat. No. 303710, Dilution 1:200) and CD42b (PE ...
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bioRxiv - Immunology 2024Quote: ... cells were harvested and stained for 30min at 4°C with anti-CD25 APC (Biolegend, Cat: 102012, 1:50), and ZombieUV (Biolegend ...
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bioRxiv - Systems Biology 2023Quote: ... The resulting single cells suspension was used to detect leukocyte populations (CD45 BV421 [clone: 104; Biolegend], CD4 PerCPeFluor710 [RM4-5; eBioscience], CD8a APC-Cy7 [53-6.7; Biolegend], CD19 PE-Cy7 [6D5; Biolegend]) and CD4+ Foxp3+ Treg (CD45 BV421 ...
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bioRxiv - Cell Biology 2024Quote: ... cells were labeled with antibodies detecting the following cell-surface markers: CD133-APC (Biolegend, clone 7, dilution 1:20), CD19-PECy7 (Biolegend ...
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bioRxiv - Bioengineering 2024Quote: ... and stained with fluorescently-labeled antibodies (i.e., PE/Cy7-CD11c, APC-MHC II, PE-CD86, and FITC-CD40 – Biolegend) for 30 minutes ...
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bioRxiv - Bioengineering 2024Quote: ... CD8a (clone 53-6.7, PE), CD206 (clone C068C2, PE-Cy5), and Ly6G (clone 1A8, APC) were purchased from BioLegend. Deionized water was of ultrapure grade obtained through a Milli-Q water system (EMD Millipore).
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bioRxiv - Bioengineering 2024Quote: ... supernatant was removed for analysis with ELISA MAX Deluxe Set Human IL-2 and ELISA MAX Deluxe Set Human IFN-γ (BioLegend). Cytotoxicity at the various indicated timepoints was tested using a luciferase-based assay ...
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bioRxiv - Immunology 2024Quote: ... cells were co-incubated with human CD16-expressing NK cells (NK92humCD16) for 4 hours at 37°C and the 421™ anti-human CD107a (Biolegend) antibody as a degranulation marker ...
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bioRxiv - Immunology 2024Quote: ... Alexa Fluor® 594 anti-mouse/human Ki-67 and Alexa Fluor® 647 anti-mouse/human CD324 (E-cadherin) antibodies (#801203, # 151213 and #147308, BioLegend). Each batch of anti-tubulin β3 antibody was titrated before use to minimize background staining ...
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bioRxiv - Immunology 2024Quote: ... B cells were isolated using the EasySep Human B Cell Isolation Kit (StemCell #17954) or the MojoSort Human Pan B Cell Isolation Kit (BioLegend #480082) according to the manufacturer’s instructions ...
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bioRxiv - Cancer Biology 2023Quote: ... PDX tumors were digested to a single cell suspension in Collagenase digest buffer and sorted for human cells using anti human HLA-ABC (Biolegend, 555555), DNA and RNA were extracted using an AllPrep Kit (Qiagen ...
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bioRxiv - Immunology 2024Quote: ... B cells were isolated by negative selection using the EasySep Human B Cell Isolation Kit (StemCell, #17954) or the MojoSort Human Pan B Cell Isolation Kit (BioLegend, # 480082) according to the manufacturer’s instructions.