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Citations for BioLegend :
301 - 350 of 604 citations for Sulfur Free Cobalt Metallo Organic Standard Co @ 5000 µg g in Hydrocarbon Oil since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: The cytokines IFNγ and TNFα contained in supernatants derived from 1-4 h BxPC3 - CAR T cell co-cultures were quantified by ELISA using Abs from Biolegend.
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bioRxiv - Immunology 2021Quote: ... Cell viability staining was routinely performed for plate stimulations and U87 co-culture using fixable violet or near-infrared viability dyes (Zombie UV fixable viability kit [Biolegend] ...
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bioRxiv - Immunology 2020Quote: ... Cell viability staining was routinely performed for plate stimulations and U87 co-culture using fixable violet or near-infrared viability dyes (Zombie UV fixable viability kit [Biolegend] ...
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bioRxiv - Cancer Biology 2023Quote: ... Supernatants were collected from effector-target cell co-cultures and analyzed for IFNy using either ELISA MAX Deluxe Human IFNγ kit (BioLegend) or Human IFNγ DuoSet ELISA (R&D Systems ...
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bioRxiv - Immunology 2023Quote: ... along with co-stimulatory antibodies (CD28 and CD49d at 1 μL/mL) and Brefeldin-A (1 μL/mL, BioLegend, USA). LIVE/DEAD and surface staining was performed as described above ...
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bioRxiv - Bioengineering 2024Quote: ... After 48h of incubation at 37 °C and 5% CO2 the co-cultures were prepared for flow cytometry analysis by staining for CD3 (cat. # 317318, Biolegend) and viability (DAPI ...
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bioRxiv - Immunology 2021Quote: ... B16-F1 and A375 cells were treated using carrier-free recombinant mouse (Biolegend) or human (SinoBiological ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed with pre-warmed serum-free media and TGF-β (Biolegend) was added in a final concentration of 1 ng/mL ...
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bioRxiv - Bioengineering 2023Quote: ... GM-CSF (20 ng/mL; recombinant Mouse GM-CSF (carrier-free) from BioLegend) ...
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bioRxiv - Bioengineering 2023Quote: ... GM-CSF (20 ng/mL; recombinant Mouse GM-CSF (carrier-free) from BioLegend) ...
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bioRxiv - Immunology 2024Quote: ... and 100ng/mL final concentration of recombinant human Flt3L (carrier-free formulation, BioLegend). Plates were briefly centrifuged and then imaged at 6-hour intervals for mCherryNLS fluorescence using the BioTek Cytation 7 Cell Imaging Multimode Reader (Agilent ...
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bioRxiv - Neuroscience 2020Quote: ... These microglia were labeled with FITC anti-mouse CD45 (2 µg/mL, Biolegend, USA), APC anti-mouse CD11b (5 µg/mL ...
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bioRxiv - Developmental Biology 2021Quote: ... rat anti-mouse CD31 (labelled with AF647) (BioLegend – 102516; 0.25 µg per million cells) and rat anti-mouse CD45 (labelled with V500 ...
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bioRxiv - Neuroscience 2021Quote: ... The primary antibodies used were CD45 (Clone 30-F11, FITC, 0.6 µg/ml, Biolegend), CD11b (Clone M1/70 ...
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bioRxiv - Immunology 2020Quote: 2 µg of primary conjugated anti-mouse Ly6G-Alexa Fluor 647 (clone 1A8, Biolegend) diluted into 200 µl PBS were injected intravenously via tail vein ten minutes prior to imaging as previously published [15,16].
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bioRxiv - Immunology 2021Quote: ... and biotinylated anti-mouse CD49b antibody (25 µg/ 1×108 via.cells, clone DX5, Biolegend) per each kits’ instructions ...
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bioRxiv - Immunology 2022Quote: ... cells were treated with 10 µg/ml anti-IL-10R antibody (clone 1.3B1a, BioLegend) for 30 min prior to IL-10 stimulation ...
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bioRxiv - Immunology 2024Quote: ... Cells were resuspended in FACS buffer containing 0.5 µg/mL 7-AAD (Biolegend, 420403) and incubated on ice for 10 minutes and then acquired directly on the Attune NxT flow cytometer (Thermofisher Scientific) ...
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bioRxiv - Developmental Biology 2024Quote: ... 2 µL (1 µg) of the corresponding hashing antibody (Totalseq-A, Biolegend, Table S4) were added to the blocked samples ...
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bioRxiv - Immunology 2024Quote: ... plates were coated with 2 µg/mL of anti-IgE (clone RME-1, BioLegend) and for IgG1 ELISAs ...
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bioRxiv - Immunology 2024Quote: ... with 3 µg of APC-labeled anti-CD8b.2 antibody (clone 53-5.8, Biolegend) 5 min prior to euthanasia to mark vasculature-associated cells in vivo.
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bioRxiv - Immunology 2024Quote: Plates were coated with 1.25 µg/ml anti-CD3 (145-2C11 or OKT3, Biolegend) with or without 10 µg/ml Fc proteins (R&D Systems Inc.) ...
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bioRxiv - Immunology 2023Quote: Mice were treated with 50 µg anti-CD3 Ultra-LEAF (Clone 145-2C11) (Biolegend) or sterile PBS (Gibco ...
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bioRxiv - Immunology 2023Quote: ... Staining was performed using 5 µg/ml anti-human CD106 APC Antibody (Biolegend, USA) or 5 µg/ml mouse IgG2a kappa Isotype control (eBM2a ...
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bioRxiv - Immunology 2023Quote: ... cells were incubated with 200 µg/mL recombinant Galectin-1 and Galectin-3 (Biolegend). Galectin binding was competitively inhibited by adding 50 mM lactose during the staining procedure.
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bioRxiv - Cell Biology 2024Quote: ... We coated 100mm tissue culture dishes with 42 µg anti-Mouse CD45 (BioLegend, 103101) and 16 µg anti-Mouse CD32 (BioLegend ...
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bioRxiv - Cancer Biology 2024Quote: ... Cells were stimulated with anti-CD3/CD28 (3.5 µg/ml anti-CD3 mAb, BioLegend, #100339 ...
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bioRxiv - Immunology 2024Quote: ... TotalSeq-C0255) (0.5 µg per 1,000,000 cells) and TotalSeq-C0050 anti-CD19 antibody (BioLegend) for demultiplexing individual samples in downstream analysis ...
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bioRxiv - Synthetic Biology 2024Quote: ... Alexa Fluor® 647 anti-mouse CD86 Antibody (0.125 µg/sample; Biolegend; cat#105020) and FITC anti-mouse CD206 (MMR ...
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bioRxiv - Immunology 2024Quote: ... and 0.333 µg/mL rat-anti-mouse CD8alpha-APC (clone 53-6.7, 100711, Biolegend) for 30 min at 4 °C ...
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bioRxiv - Immunology 2024Quote: ... mice were injected intravenously with 1 µg of CD45-PE antibody (30-F11, Biolegend) 5 min before sacrifice.
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bioRxiv - Immunology 2024Quote: ... 1.3×106 PBMCs/well were seeded with 1 µg/mL soluble αCD28 (CD28.2, Biolegend) in 1 mL Iscove’s Modified Dubecco’s Medium (IMDM ...
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bioRxiv - Cancer Biology 2024Quote: ... mice were treated with 150 µg/mice anti-PD1 (clone 29F.1A12, BioLegend, 135248) or isotype control (clone RTK2758 ...
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bioRxiv - Neuroscience 2021Quote: ... The exception is that secondary labeling was accomplished using anti-rat DyLight 405 (1:5000, Novus, NBPI-72983) or anti-mouse DyLight 405 (1:1000, BioLegend, Poly-24091). Fixed cells were then imaged on a Nikon Eclipse TE2000 inverted microscope using a 60x 1.49NA oil-immersion TIRF objective and 405nm and 560nm solid lasers using a CoolSNAP HQ2 CCD camera ...
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bioRxiv - Plant Biology 2021Quote: ... Biotin labeled DNA was detected with 1:5000 solution of an anti-biotin HPR-conjugated antibody (BioLegend, San Diego, California, United States) in TBST with 5% BSA ...
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bioRxiv - Microbiology 2023Quote: ... Tissue was rinsed 3 times in 1X PBS and then incubated with 1:5000 dilution rabbit polyclonal anti-Keratin-14 antibody (Biolegend, Clone Poly19053) for 1 hour and then detected with 1:10000 dilution goat anti-Rabbit IgG Secondary Antibody conjugated with Alexa Fluor™ 488 (ThermoFisher Scientific ...
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bioRxiv - Immunology 2021Quote: ... Secreted cytokines were measured with ELISA MAX Standard Set Mouse or Human kits according to the manufacturer’s protocol (BioLegend). Cellular viability was determined by resuspending cells in cRPMI with 0.1mM resazurin (Sigma Aldrich ...
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bioRxiv - Pathology 2022Quote: Serum levels of IL-6 and TNFα were measured by a mouse ELISA MAX Standard IL-6 kit (BioLegend) and a mouse ELISA MAX Standard TNFα kit (BioLegend).
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bioRxiv - Microbiology 2023Quote: ... Detection of IgE from mouse serum was performed by ELISA using ELISA MAX™ Standard Set Mouse IgE (Biolegend) according to the manufacturer’s instructions.
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bioRxiv - Immunology 2023Quote: ... The supernatants were obtained and IFN-γ and TNF-α concentrations were quantified using ELISA MAX Standard Sets (Biolegend; cat.# 430801 for IFN-γ ...
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bioRxiv - Immunology 2023Quote: ... Spleen standard cell suspensions were stained in the same conditions but used anti-CD45-FITC (Biolegend 109806, clone 104) to distinguish it from the other samples once pooled ...
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bioRxiv - Immunology 2024Quote: ELISA targeting IFN-γ were conducted using the ELISA MAX™ Standard Set Human IFN-γ (Biolegend, California, USA). The procedure was done according to the manufacturers protocol with slight alterations ...
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bioRxiv - Bioengineering 2024Quote: The T cells generated under standard conditions were stained on day 5 of culture with the antibodies Thy1.1-APC (Biolegend) and pan-TCR-α/β-PE (Beckman Coulter) ...
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bioRxiv - Immunology 2022Quote: ... or anti-VSV G (1:1,000) in Intracellular Staining Permeabilization Wash Buffer (Biolegend) combined with Alexa Fluor (AF ...
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bioRxiv - Microbiology 2020Quote: ... Protein G agorose beads that were pre-adsorbed to either anti-HA (BioLegend) or anti-FLAG (Millipore ...
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bioRxiv - Immunology 2021Quote: ... cells were stained with anti-IFN-g or anti-IL-17A antibodies (Biolegend); For intracellular FoxP3 staining ...
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bioRxiv - Cancer Biology 2021Quote: Cells were harvested at the indicated times and co-stained with Annexin-V-FITC and propidium iodide following manufacturer instructions (BioLegend, #640914). To assess active Caspase-3 ...
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bioRxiv - Cancer Biology 2022Quote: ... LEPs from co-cultures were separated after 10 days for gene expression qPCR analysis by FACS using anti-human CD133-PE (BioLegend, clone7) and anti-human CD271-APC (BioLegend ...
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bioRxiv - Cancer Biology 2023Quote: ... co-culture samples were collected at 120h and stained for live/dead fixable Aqua and CD3 (Clone HIT3a, Biolegend, Cat# 300306) in FACS buffer ...
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bioRxiv - Microbiology 2022Quote: ... Endotoxin-free recombinant mouse IL-1α and IL-1β proteins were purchased from BioLegend.