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Citations for BioLegend :
501 - 550 of 604 citations for Sulfur Free Cobalt Metallo Organic Standard Co @ 5000 µg g in Hydrocarbon Oil since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2023Quote: ... Cell surface expression level of syndecan-1 was evaluated by incubating cells with anti-syndecan-1 antibody (10 µg/ml, B280312, Biolegend) for 1h at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... Following combinations of cytokines and antibodies were used for the differentiation of T cells: for Th1 cells - 1 µg/ml anti-IL-4 (clone 11B11; Biolegend), 30 U/ml rhIL-2 (NIH) ...
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bioRxiv - Microbiology 2023Quote: ... for 30 minutes at 37°C and then incubated with 10 µg/ml of αASC TMS-1 (clone HASC-71, Biolegend) in blocking buffer ...
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bioRxiv - Cancer Biology 2023Quote: ... PBMCs and HeLa target cells were co-cultured in u-bottom 96 well plates at a range of T:E ratios (indicated in figure legends) T cells were stimulated with 10 µg/mL plate-immobilised anti-CD3 (OKT3, BioLegend) and cultured for 4 days prior to staining with anti-CD8-APC (SK1 ...
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bioRxiv - Cell Biology 2023Quote: ... injection of 500 mg/kg), and vascular markers (20 µg) of interest conjugated to AF647 (CD31, ICAM1, ICAM2, and CD36 (BioLegend), P-selectin (BD Pharmingen)) ...
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bioRxiv - Cancer Biology 2023Quote: ... 5 x105 cells were labeled with 1 μg anti-hCD81-PE clone 5A6 or 1 µg isotype control-PE (349506, 400114; Biolegend)32 and by Western blotting (4-12% SDS-PAGE ...
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bioRxiv - Immunology 2023Quote: ... naïve CD8+ T cells were cultured in RPMI (10% FCS) and stimulated with plate-bound mCD3 mAbs (3 µg ml-1, clone 145-2C11, Biolegend), soluble mCD28 (0.5 µg ml-1 ...
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bioRxiv - Immunology 2024Quote: ... and 2.5 mM HEPES into 6-well plates pre-coated for more than 1 h with 5.4 µg of Ultra-LEAFTM purified anti-mouse CD3e antibody (clone 145-2C11, Biolegend, # 100340) and 3 µg of Ultra-LEAFTM purified anti-mouse CD28 antibody (clone 37.51 ...
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bioRxiv - Immunology 2024Quote: ... cells were treated with 10 µg/ml of Ultra-LEAFTM purified anti-mouse IFNγ antibody (clone R4-6A2, Biolegend, #505709), 10 µg/ml of Ultra-LEAFTM purified anti-mouse TNF antibody (clone MP6XT22 ...
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bioRxiv - Immunology 2024Quote: ... and 5 µg/mL of soluble Ultra-LEAF™ purified anti-human CD28 monoclonal antibody (clone 28.2, BioLegend, cat. 302933) in R10 medium [RPMI-1640 medium supplemented with 10% heat-inactivated FBS ...
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bioRxiv - Immunology 2024Quote: ... CTLs were seeded on sapphire discs coated with poly-L-ornithine (0.1 mg/ml) and anti-CD3 UCHT1 (30 µg/ml, BioLegend # 300402) in flat specimen carriers (Leica) ...
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bioRxiv - Immunology 2024Quote: ... which were coated with poly-L-ornithine (0.1 mg/ml) and mouse anti-CD3 UCHT1 (30 µg/ml, BioLegend # 300402), for 10 min at RT (20°C ± 2°C ...
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bioRxiv - Immunology 2024Quote: ... Tregs were split and incubated at a concentration of 1 x 106 cells/mL cRPMI for 30 minutes at 37°C in cRPMI with 10 µg/mL anti-CD226 mAb (BioLegend) or IgG2a isotype control (BioLegend) ...
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bioRxiv - Immunology 2024Quote: Naïve CD4+ T cells isolated from WT mice spleen and LN were treated with or without 10 µg/ml anti-β2 integrin non-function blocking antibody (BioLegend) for 1 hour and added onto 96 well plate coated with anti-CD3/CD28 along with either 2.5µg/ml of rLum or rBgn or rDcn or 1µg/ml of ICAM-1 for 30 minutes to check phospho-Akt-1 expression or 6 hours to check activation CD69 expression or 72 hours to check proliferation (CD4+ T cells were pre-labeled with CFSE for proliferation assay) ...
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bioRxiv - Immunology 2024Quote: ... 0.3 x 106 OT-I T cells were placed on flat-bottom 96 well plates coated with 5 µg/mL anti-CD3ε (clone 145-2C11; Biolegend) and stimulated with 1 µg/mL anti-CD28 mAb (clone 37.51 ...
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bioRxiv - Genomics 2024Quote: ... and Fc receptors were blocked with 0.5 µg of TruStain FcX PLUS (anti-mouse CD16/32, BioLegend Cat. No. 156603) for 10 minutes on ice ...
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bioRxiv - Bioengineering 2020Quote: ... and treated with Fc-receptor blocker (2.5 μ g/mL, anti-mouse CD16/32; BioLegend, Cat# 101301, RRID: AB_312800) for 20 minutes at 4 °C in the dark ...
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bioRxiv - Cell Biology 2022Quote: ... 1µg/mL for FC) APC mouse IgG1, k isotype Control (400120, 1µg/mL for FC) were purchased from Biolegend.
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bioRxiv - Immunology 2022Quote: ... in the presence of plate-bound anti-CD3 (1 μg/ml) and soluble anti-CD28 (0.5 μg/ml) supplemented or not with IL-27 (30 ng/ml, BioLegend). Proliferation of Tconv cells was determined by CellTrace Violet Dye dilution in FACS analysis ...
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bioRxiv - Systems Biology 2022Quote: Sorted live single cells were centrifuged at 400 x g for 5min at 4°C and resuspended in 25μL of cell staining buffer (BioLegend). Human TruStain FcX FC blocking reagent (BioLegend ...
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bioRxiv - Cancer Biology 2023Quote: ... C57BL/6J mice bearing orthotopic KPC tumors were treated with 10 μg g−1 anti-TNF-α antibody (BioLegend) in combination with 100 mg/kg gemcitabine (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2024Quote: ... Supernatants were collected (spun down at 10,000 x g for 15 mins) for cytokine analysis using LegendPlex 13-plex Mouse Inflammation Panel (Biolegend). Adhered cells were washed with 1x PBS and collected using 0.25% trypsin ...
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bioRxiv - Bioengineering 2024Quote: ... anti-mouse IgG2a/b-FITC (5 g/ml) and anti-mouse CD138-PE (4 ug/ml; BioLegend cat# 142504); ii ...
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bioRxiv - Immunology 2021Quote: ... cell-free supernatants were collected followed by ELISA to detect IL-10 and TGFβ using capture and detecting antibodies (Biolegend). Other M2 phenotype characteristics including the cell surface expressions of CD206 ...
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bioRxiv - Cancer Biology 2022Quote: Single-cell suspensions were washed after collection and stained in ice-cold Ca+2/Mg+2-free PBS with a viability dye (Zombie NIR, BioLegend) for 20 min at 4°C in the dark ...
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bioRxiv - Immunology 2024Quote: Secretion of soluble cytokines and chemokines were assayed from cell-free culture supernatants of in vitro stimulated neutrophils and monocytes using bead-based immunoassays (LegendPlex, Biolegend). Two assays were used allowing the quantification of IL-1β ...
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bioRxiv - Immunology 2023Quote: ... Cells were lysed in RLT buffer and supernatants were collected for analysis using ELISA (Total Tgf-β1 Legend MAX and Free Active Tgf-β1 Legend MAX, Biolegend). The limit of detection was 8 pg/mL for Tgf-β1.
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bioRxiv - Physiology 2023Quote: Kupffer cells cultured in 96-well plates were supplemented with 30 μg EPO per well (recombinant rat EPO carrier-free, Biolegend). After 1,5 hours ...
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bioRxiv - Cancer Biology 2023Quote: ... Jurkat transfectants were activated for 5 minutes at 37°C in serum-free RPMI-1640-sodium bicarbonate by crosslinking the anti-CD3 antibody (1μg/ml, clone UCHT1, BioLegend #300402) with secondary anti-mouse antibodies for immunoblot analysis of GSK-3 α/β and Akt phosphorylation.
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bioRxiv - Immunology 2023Quote: ... Tgf-β concentration was measured using ELISA (Total Tgf-β1 Legend MAX and Free Active Tgf-β1 Legend MAX, Biolegend). The limit of detection was 8 pg/mL for Tgf-β1 ...
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bioRxiv - Cell Biology 2024Quote: ... Excess media was eluted off and replaced with serum-free media containing typically 250 nM MitoSpyTM Red CMXRos (424801, BioLegend)(in some experiments MitoTracker was used instead ...
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bioRxiv - Neuroscience 2024Quote: ... The remaining 80% cleared supernatant was transferred to a clean RNase-free tube and incubated with mouse anti-HA antibody (1:250; clone 16B12, Biolegend) on a rotating mixer for 4 h at 4°C while 300 uL protein G magnetic beads (Dynabeads ...
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bioRxiv - Developmental Biology 2021Quote: ... The following primary antibodies were used for immunostaining: rat anti-EpCAM-AF647 (Biolegend, clone G8.8, 1 µg ml−1, 1:250); rabbit anti-K5 (Covance ...
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bioRxiv - Microbiology 2021Quote: ... followed by washing and incubated with a solution of AlexaFluor® 647 conjugated anti-His antibody (BioLegend #652513, 10 µg/mL). After washing and drying ...
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bioRxiv - Immunology 2022Quote: ... prM-DENV2-treated PBMCs were treated (1h, 37 °C) in the presence or absence of anti-TNF-α antibody (4 µg/mL, BioLegend). The TNF-α depleted supernatants were used to stimulate HUVEC for 6 hours and the expression of activation markers were analyzed by flow cytometry ...
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bioRxiv - Microbiology 2022Quote: ... bacteria were labeled with 5 µg ml−1 carboxylfluorescein diacetate succinimidyl ester (CFSE) or Tag-IT Violet™ proliferation and cell tracking dye (Biolegend) in PBS containing 5 mM MgSO4 ...
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bioRxiv - Cancer Biology 2022Quote: ... FACS buffer recipe was D-PBS + 5% FBS + 2% BSA + 5 mM EDTA + 50 µg/ml DNase-I with Human Trustain (Biolegend, 422302). All the compensation was done using UltraComp eBeads™ Compensation Beads (Invitrogen ...
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bioRxiv - Immunology 2022Quote: ... CD8 T cells were stimulated in presence of self-made human IL-2 on α-CD3 (5 µg/ml) (145-2C11, BioLegend), α-CD28 (5 µg/ml ...
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bioRxiv - Immunology 2024Quote: ... Some mice were intraperitoneally (i.p.) injected with Ultra-LEAF Purified anti-mouse NK-1.1 Antibody (clone#PK136; 200 µg/mouse, Biolegend, San Diego, CA) every other day ...
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bioRxiv - Immunology 2024Quote: ... the harvested cells were restimulated using a pool of overlapping Wuhan Spike peptides (Miltenyi, 130-126-701) at 2 µg/mL for 16 hours in the presence of brefeldin A (Biolegend, #400602) at 5 µg/mL ...
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bioRxiv - Immunology 2023Quote: ... HTO stained PBMCs from all 10 donors were then pooled and stained with oligo-tagged TotalSeq C antibody for cell surface proteins for T cell memory markers (CD45RA, CCR7, CD95 and IL7Rα) (0.5 µg/condition; Biolegend, Table S2B) for 30 minutes on ice ...
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bioRxiv - Bioengineering 2024Quote: Resting T-cells were cultured in RPMI-1640 media supplemented with 5 µg/mL brefeldin A (BioLegend, San Diego, CA, USA). After 24 hours of cultivation ...
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bioRxiv - Cell Biology 2024Quote: Single-cell suspensions were pre-incubated with 1 µg/mL of aCD16/32 Fc-Block (BioLegend, San Diego, CA, USA, 101310) in 300 µL staining buffer for 10 min ...
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bioRxiv - Bioengineering 2024Quote: ... were coated with 100 µL of 4 µg/mL polyclonal anti-lysostaphin antibody (AICBIOTECH-PAb102) in 1×ELISA coating buffer (BIOLEGEND-421701). Wells were washed with 0.05% Tween 20 (SIGMA-P9416-50ML ...
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bioRxiv - Microbiology 2023Quote: ... the cells were incubated with Alexa Fluor® 488-labeled anti-human CD69 antibody or an isotype control (1 µg/ml, BioLegend) for 45 min ...
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bioRxiv - Cancer Biology 2023Quote: ... centrifuged and 500,000 cells were stained in 200 µl 1x PBS plus 0.5 µg of indicated antibody for 30 min in the dark: APC anti-mouse CD274 (B7-H1, PD-L1, Biolegend #124311) or appropriate IgG isotype ...
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bioRxiv - Bioengineering 2023Quote: THP-1 cells were incubated with 2 µg/ml of anti-CD44 antibodies (clone: C44Mab-5; Biolegend, San Diego, CA, USA) for 20 minutes under standard cell culture conditions (95% humidity and 5% CO2 at 37°C ...
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bioRxiv - Immunology 2023Quote: ... The following antibodies were used at a concentration of 1 µg/mL except where otherwise indicated: APC-Cy7-CD117 (clone 2B8; Biolegend #105826), PE-FceRI (clone MAR-1 ...
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bioRxiv - Immunology 2023Quote: Ligand-independent reporter cell activation was assessed by overnight stimulation with 6.25 µg mL-1 Ultra-LEAF™ Purified anti-human CD3 (OKT3, BioLegend, #317326). Cells were incubated in antibody-coated wells and TCR responses were measured by flow cytometric analysis of GFP expression after 16h ...
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bioRxiv - Immunology 2023Quote: ... For the memory B cell EliSpot, PBMC were stimulated with 1 µg/ml R848 (SigmaAldrich, SML0196) and 10 ng/ml recombinant human IL-2 (Biolegend, 791904) for 5 days before harvesting and plating of 50-100.000 cells/well ...