5 Benzyloxy pyrimidine 2 carbonitrile citations for BioLegend

Quote: ... anti-IL-2 BV650 (clone MQ1-17H12; 5 μL; cat# 500334) and anti-IFNγ PE/Dazzle 594 (clone B27; 5 μL; cat# 506530) both from Biolegend; anti-IL-17a Alexa Fluor 488 (clone eBio64DEC17 ...

Leishmania amazonensis infection induces PD-L1 expression on dendritic cells in an mTor-dependent manner and impairs Th1 responses in vitro and in vivo

Herbert L. de Matos Guedes, et al., bioRxiv - Immunology 2022

Quote: ... or DC in vitro culture (5×105) were washed with PBS at 400 g for 5 min at 4°C and blocked with FcX (BioLegend) for 15 min ...

CryoSCAPE: Scalable Immune Profiling Using Cryopreserved Whole Blood for Multi-omic Single Cell and Functional Assays

Alexander T. Heubeck, et al., bioRxiv - Immunology 2024

Quote: ... or 5 uL of Mojosort Human Dead Cell Removal Beads and 5 uL of Mojosort Human anti-PE Nanobeads (BioLegend) for two round bead depletion ...

Distal super-enhancer drives aberrant CXCL13 expression in Cancer cells driving growth and p53 dysregulation via CXCR5-CXCL13 axis

Santosh Kumar Gothwal, et al., bioRxiv - Cancer Biology 2024

Quote: ... was filled with RPMI (5% FBS) or RPMI (5% FBS) medium containing rhCXCL12 (200 ng/ml) or rhCXCL13 (1000 ng/ml) (Biolegend). Cell suspension of 5*105 cells or 1.5*105 cells for normal human B cells ...

Stress Induced Signaling Pathways in Burkitt’s Lymphoma Play Novel Mechanisms in Fate Determination and Pathogenesis of Germinal Center-Derived B-Lymphomas

Santosh K Gothwal, Jacqueline H Barlow, bioRxiv - Cancer Biology 2024

Quote: ... REF #3421) containing RPMI medium (5% FBS) or RPMI medium (5% FBS) supplemented with rmCXCL13 (1000 ng/ml) (BioLegend #583902) for 5 minutes ...

Surface-associated antigen induces B-cell permeabilization and lysosome exocytosis facilitating antigen uptake and presentation to T-cells

Fernando Y. Maeda, et al., bioRxiv - Cell Biology 2021

Quote: ... the concentration of IL-2 in the supernatant was measured using an IL-2 ELISA kit (Biolegend).

State-dependent protein-protein interactions mediating 4-1BB CAR Signaling

Samuel A. Ritmeester-Loy, et al., bioRxiv - Immunology 2022

Quote: ... IL-2 concentration in harvested supernatant was then analyzed using Human IL-2 ELISA Max Kit (BioLegend) per provided instruction ...

Inosine Induces Stemness Features in CAR T cells and Enhances Potency

Dorota D. Klysz, et al., bioRxiv - Immunology 2023

Quote: ... and interleukin-2 (IL-2) and interferon-ψ concentrations were determined by enzyme-linked immunosorbent assay (BioLegend). Triplicate wells were plated for each condition.

Targets of influenza Human T cell response are mostly conserved in H5N1

John Sidney, et al., bioRxiv - Immunology 2024

Quote: ... while CD137 APC (2:100) and OX40 PE-Cy7 (2:100; Biolegend; clone Ber-ACT35; Cat# 350012) were used for CD4+ T cells.

Evolutionary fingerprint in rodent PD1 confers weakened activity and enhanced tumor immunity compared to human PD1

Takeya Masubuchi, et al., bioRxiv - Immunology 2024

Quote: ... Secreted IL-2 in the supernatant was measured using Human or Mouse IL-2 ELISA Kit (BioLegend). All incubations were performed at 37 °C / 5% CO2.

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