5 Azepane 1 sulfonyl 2 methoxy benzoic acid citations for BioLegend

Quote: ... Tetramers were prepared by mixing 1 or 5 pmol of biotinylated protein monomer with 0.25 to 1.25 pmol of streptavidin-PE (Biolegend #405245) respectively and incubating for 2 hours at room temperature ...

Cryo-EM structures of α-synuclein filaments from Parkinson’s disease and dementia with Lewy bodies

Yang Yang, et al., bioRxiv - Neuroscience 2022

Quote: ... Primary antibodies were: Syn303 [a mouse monoclonal antibody that recognizes residues 1-5 of human α-synuclein (50)] (BioLegend) at 1:4,000 ...

Naïve and in vitro-activated primary mouse CD8⁺ T cells retain in vivo immune responsiveness after electroporation-based CRISPR/Cas9 genetic engineering

Petra Pfenninger, Laura Yerly, Jun Abe, bioRxiv - Immunology 2021

Quote: ... and seeded in the antibody-coated flask at 3–5×105 cells/cm2 together with soluble 1 µg/mL anti-CD28 antibodies (clone 37.51; 102116, BioLegend). Cells were cultured for 48 hr at 37°C in a humidified 5% CO2 atmosphere ...

IDENTIFICATION OF A NOVEL MECHANISM FOR LFA-1 ORGANIZATION DURING NK CYTOLYTIC RESPONSE

Alejandro P. Pariani, et al., bioRxiv - Cell Biology 2022

Quote: ... poly-l-lysine coated glass slides were coated by overnight incubation with ICAM-1 (5 μg/mL, Biolegend 552906) or with ICAM-1 and anti-CD28 (5 μg/mL ...

Type 1 interferon remodels normal and neoplastic hematopoiesis in human

Chhiring Lama, et al., bioRxiv - Cancer Biology 2022

Quote: ... Either TotalSeq-A Anti-Nuclei Pore Complex Proteins Hashtag 9 or 10 Antibody (1uL at 1:5 dilution; BioLegend) was added to each aliquot of lysis buffer ...

New SNCA mutation and structures of α-synuclein filaments from juvenile-onset synucleinopathy

Yang Yang, et al., bioRxiv - Neuroscience 2022

Quote: ... Primary antibodies were: Syn303 [a mouse monoclonal antibody that recognises residues 1-5 of human α-synuclein (7)] (BioLegend) at 1:4,000 ...

Olfactory learning in Drosophila requires O-GlcNAcylation of mushroom body ribosomal subunits

Haibin Yu, et al., bioRxiv - Cell Biology 2023

Quote: ... Primary antibodies were dissolved in 5% BSA (Biofroxx, 4240GR005) and the dilutions were: Streptavidin-Cy3 (1:200, BioLegend, 405215), RL2 (1:200 ...

Single-cell multidimensional profiling of tumor cell heterogeneity in supratentorial ependymomas

Daeun Jeong, et al., bioRxiv - Cancer Biology 2024

Quote: ... centrifuged at 500g for 5 minutes and resuspended in a solution of 1% bovine ser albumin (BSA) (Biolegend, 644710) in phosphate-buffered saline (PBS ...

Quality of vaccination-induced T cell responses is conveyed by polyclonality and high, but not maximum, antigen receptor avidity

Katharina Kocher, et al., bioRxiv - Immunology 2024

Quote: ... and individual hashtag antibodies (2.5 μL per 5×106 PBMCs of TotalSeq-C anti-human hashtag antibodies 1–8, BioLegend, 394661 ...

Quality of vaccination-induced T cell responses is conveyed by polyclonality and high, but not maximum, antigen receptor avidity

Katharina Kocher, et al., bioRxiv - Immunology 2024

Quote: ... individual hashtag antibodies (2.5 μL per 5×106 PBMCs of TotalSeq-C anti-human hashtag antibodies 1–8, BioLegend, 394661 ...

Deep immune profiling of the maternal-fetal interface with mild SARS-CoV-2 infection

Suhas Sureshchandra, et al., bioRxiv - Immunology 2021

Quote: Freshly thawed decidual immune cells (1-2 10e6 cells) were incubated with Fc blocker (Human TruStain FcX, Biolegend) in PBS with 1% BSA for 10 minutes at 4C and then surface stained with CD45-FITC (HI30 ...

Human cytomegalovirus infection changes the pattern of surface markers of small extracellular vesicles isolated from first trimester placental histocultures

Mathilde Bergamelli, et al., bioRxiv - Cell Biology 2020

Quote: ... mouse anti-Vimentin (Santa-Cruz; 2 μg/mL) and mouse anti-placental alkaline phosphatase (Biolegend; 1 μg/mL). Immunostaining for hCMV was performed as previously described [32] ...

The lncRNA landscape of cardiac resident macrophages and identification of Schlafenlnc as a regulator of macrophage migratory function

Anne Dueck, et al., bioRxiv - Cell Biology 2022

Quote: ... Both cell fractions were stained with cell hashing antibodies (1:1000, #2 for heart, #3 for blood, Biolegend) and Propidium iodide (1:100 ...

LAT encodes T cell activation pathway balance

Adam J. Rubin, et al., bioRxiv - Genomics 2024

Quote: ... and resuspended in 50ul PBS plus 2% FBS containing 1:100 diluted anti-CD69-APC antibody (BioLegend 310909). Cells were stained in the dark on ice for 30 min ...

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