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Citations for BioLegend :
451 - 500 of 8337 citations for 5 Azepane 1 sulfonyl 2 methoxy benzoic acid since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: Splenocytes from TregΔCd226 and TregWT mice were plated in a 96-well plate at a concentration of 1,000,000 cells/mL with plate-bound α-CD3ε (2 μg/mL) and plate-bound CD155 (1 μg/mL; BioLegend) for 15 or 30 minutes at 37ºC ...
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bioRxiv - Immunology 2020Quote: ... and then plated into 12 well plates previously coated for 2 hours RT with 1 μg/ml of huCD28.2 (BioLegend) and 1 μg/ml of human CD3ε (BioLegend) ...
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bioRxiv - Cancer Biology 2021Quote: ... single cell suspensions were resuspended in FACS buffer (PBS, 2%FBS, 0.01%Tween) and stained for viability dye (1/1000, Biolegend) in 100ul PBS for 10min at RT followed by surface staining with antibody specific for CD45 (30-F11 ...
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bioRxiv - Immunology 2021Quote: ... with the caveat that CD8+ enrichment was achieved by incubating 1-2 million PBMCs with CD8 Mojobeads (Biolegend 480108) for 15 minutes on ice ...
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bioRxiv - Immunology 2022Quote: ... enriched CD8 T cells were incubated for 10’ at 4°C in Staining buffer (2%BSA/0.01% Tween in PBS) containing FcBlock (1/10 dilution ;TruStainTM FcX, Biolegend) followed by a 30’ incubation in Staining Buffer containing TotalSeqB antibodies against Ly108 ...
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bioRxiv - Cell Biology 2024Quote: ... Membranes were blocked overnight in Odyssey PBS Blocking Buffer (Li-Cor) and stained for 2 hours at RT with Syn1 (1:1,000; Biolegend) or γH2AX (1:1,000 ...
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bioRxiv - Immunology 2024Quote: ... then after 2 washes cells were incubated with streptavidin (SA) conjugated to Brilliant Violet 650 (BV650, 1:200, BioLegend) in PBS on ice for 15 minutes ...
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bioRxiv - Immunology 2023Quote: ... cells were stained for sorting in PBS containing 2% FCS at 4 °C with the following antibodies: CD271-PE (1:200; ME20.4, BioLegend), CD235a-PECy7 (1:20 ...
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bioRxiv - Immunology 2021Quote: ... in PBS with 2% FCS and 2% EDTA (FACS buffer) with Fc block (Biolegend) for 25 minutes ...
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bioRxiv - Immunology 2023Quote: ... and 2 ng/ml human interleukin (IL)-2 recombinant (BioLegend, San Diego, CA, 589104).
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bioRxiv - Immunology 2023Quote: ... IL-2 concentration in thawed supernatant was measured by ELISA (Biolegend, IL-2 human). For experiments with KIR2DS1 reporter cells ...
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bioRxiv - Immunology 2024Quote: ... Media included recombinant human interleukin-2 (IL-2) at 230IU/mL – 1,025IU/mL (Biolegend), with the lower concentrations used on days 1-2 ...
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bioRxiv - Microbiology 2022Quote: ... bacteria were labeled with 5 µg ml−1 carboxylfluorescein diacetate succinimidyl ester (CFSE) or Tag-IT Violet™ proliferation and cell tracking dye (Biolegend) in PBS containing 5 mM MgSO4 ...
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bioRxiv - Immunology 2022Quote: ... single-cell spleen suspensions from C57BL/6J mice were cultured at 5 million cells/ mL in complete RPMI with 1 μg/mL anti-CD3 (BioLegend, #100340) and 1 μg/mL anti-CD28 (BioLegend ...
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bioRxiv - Neuroscience 2023Quote: ... Membranes were incubated in primary antibodies diluted in 5% skim milk in TBST: mouse anti-brevican (1:1000, BioLegend, cat# 820101), sheep anti-neurocan (1:1000 ...
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bioRxiv - Neuroscience 2022Quote: ... Then the live microglial cells were incubated with PBS supplemented with 5% BSA + HLA-DR mouse monoclonal antibodies pre-conjugated with FITC (1:100, BioLegend, Inc.) + Hoechst 33342 (1:5000 ...
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bioRxiv - Synthetic Biology 2022Quote: ... The membrane was incubated with 5% nonfat milk in TBST for 1 hour followed by the addition of primary antibodies (BioLegend, 652505). After 1 hour of the primary antibody incubation ...
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bioRxiv - Cancer Biology 2022Quote: ... then incubated with their respective primary antibodies diluted in 5% skim milk in PBST at 4 °C overnight: purified mouse anti-βIII-tubulin (1:1,000) (BioLegend, cat. 801202), monoclonal mouse anti-GAPDH (1:50,000 ...
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bioRxiv - Synthetic Biology 2022Quote: ... The membrane was incubated with 5% nonfat milk in TBST for 1 hour followed by the addition of primary antibodies (BioLegend, 652505). After 1 hour of the primary antibody incubation ...
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bioRxiv - Immunology 2024Quote: ... diluted 1:500 in 1x Permeabilisation buffer with 5% Normal Donkey Serum at +40C and 1 h staining with DyLightTM 649 Donkey anti-rabbit IgG (BioLegend, #406406) diluted 1:500 in 1x Permeabilisation buffer ...
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bioRxiv - Immunology 2024Quote: The Fc receptors were blocked on 5X105 neutrophils resuspended in PBS with 5% FBS and TruStain FcX (1:50, BioLegend, 101320). Neutrophils were incubated with FITC conjugated anti-mouse Ly6G (1:50 ...
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bioRxiv - Microbiology 2024Quote: ... Cells were pelleted by centrifugation (2,000 rpm 5 min) and then treated with 1 mL 1X red blood cell (RBC) Lysis Buffer (Biolegend; cat# NC9067514) to lyse red blood cells ...
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bioRxiv - Biochemistry 2024Quote: ... the brain sections were treated for 1 hour at room temperature with 5 μg/ml of an anti-human Aβ antibody (6E10, BioLegend, 803015). The cells were then seeded on coverslips at a density of 3 × 105 cells and cultured for 5 days in their respective media before being fixed with 4% paraformaldehyde/sucrose for 15 minutes at room temperature ...
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bioRxiv - Cancer Biology 2024Quote: ... and conditioned for 16h at 37 °C with cell-free AKPS-supernatant (1:5) or the following mouse cytokines at a concentration of 20ng/µl: TGF-β1 (BioLegend, 763102), IL-10 (PeproTech ...
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bioRxiv - Cell Biology 2020Quote: ... Diamidino-2-Phenylindole (DAPI) (Biolegend) for live/dead exclusion and cell sorting ...
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bioRxiv - Immunology 2021Quote: ... IL-2 (MQ1-17H12, Biolegend), and IFNγ (4S.B3 ...
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bioRxiv - Immunology 2021Quote: ... and 2 μM monensin (BioLegend). For intracellular staining of IgM ...
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bioRxiv - Cancer Biology 2022Quote: ... and monensin (2 μM, Biolegend) in a humidified incubator at 37°C with 5% CO2 ...
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bioRxiv - Bioengineering 2020Quote: ... beta-2-microglobulin (BioLegend #316306) and Fixable Viability Dye eFluor780 or LIVE/DEAD Fixable Aqua Dead Cell Stain (ThermoFisher #65-0865-14 1:500 dilution ...
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bioRxiv - Immunology 2022Quote: ... and 2 nM Monensin (BioLegend) were added to each well and incubated for further 2 h ...
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bioRxiv - Immunology 2020Quote: ... anti-CD138 (281-2, Biolegend), anti-IgD (11-26c.2a ...
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bioRxiv - Genomics 2021Quote: ... IL-2 (Biolegend, Cat #500310), or CTLA-4 (Biolegend ...
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bioRxiv - Genomics 2021Quote: ... IL-2 (Biolegend, Cat #500310), or CTLA-4 (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... 2) huCD45 APC Cy7 (BioLegend), huCD3 PerCp-Cy5.5 (BioLegend) ...
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bioRxiv - Immunology 2020Quote: ... CD138 (clone 281-2, Biolegend), B220 (clone RA3-6B2 ...
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bioRxiv - Cancer Biology 2020Quote: ... CD138 (BioLegend; clone: 281-2) and analyzed for germinal center activated B cells (GCB) ...
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bioRxiv - Immunology 2020Quote: ... IL-2 PE (Biolegend 503808), CD4 PE-Cy7 (Biolegend 100528) ...
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bioRxiv - Immunology 2020Quote: ... IL-2 BV605 (Biolegend 500332), and Live/dead Near-IR (Invitrogen L10119) ...
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bioRxiv - Immunology 2021Quote: ... or -BV510 (281-2, Biolegend) and B220-BV421 (RA3-6B2 ...
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bioRxiv - Immunology 2022Quote: ... CD49d-FITC (R1-2, BioLegend), Tbet-APC (4B10 ...
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bioRxiv - Immunology 2023Quote: ... and CD138 (281-2, BioLegend), while human cells were incubated with CD20 (2H7 ...
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bioRxiv - Bioengineering 2023Quote: ... IL-2 (BioLegend, Cat. # 500326), and Perforin (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... and monensin (2 µM; Biolegend). Phorbol 12-myristate13-acetate (PMA)/ionomycin-stimulated (Cell Activation cocktail ...
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bioRxiv - Pathology 2023Quote: ... CD206 (Biolegend; clone 15-2) and CD163 (Biolegend ...
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bioRxiv - Cell Biology 2023Quote: ... mouse CD63 (NVG-2, Biolegend), CD9 (312102 ...
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bioRxiv - Immunology 2023Quote: ... IL-2 (JES6-5H4; Biolegend), TNFa-BV605 (MP6-XT22 ...
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bioRxiv - Immunology 2023Quote: ... and CADM1 (2:100, BioLegend) at 37°C for 25 min ...
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bioRxiv - Cancer Biology 2023Quote: ... and monensin (2 μM, Biolegend) in a humidified incubator at 37°C with 5% CO2 ...
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bioRxiv - Immunology 2023Quote: ... anti-CD63 (NVG-2, Biolegend) and anti-XCR1-AF647 (ZET ...
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bioRxiv - Immunology 2024Quote: ... BCL-2 (AF488, 100, Biolegend), anti-ID2 (Pe-Cy7 ...