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Citations for BioLegend :
3201 - 3250 of 8186 citations for 4 2 Hydroxy 1 Methoxyethyl 1 2 Benzenediol since 2020
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... A cocktail of Abs for surface markers at the predetermined concentrations (CD31-APC 1:100, Biolegend; CD45-APC 1:200, Biolegend, CD117-APC 1:100 ...
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bioRxiv - Immunology 2021Quote: ... at 2:1 ratio for 1 h at 4C to ensure maximum staining quality before surface staining with CD20-FITC (2H7, Biolegend) for an additional 30 minutes ...
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bioRxiv - Immunology 2021Quote: ... DC purity was assessed by antibodies against CD141 (1:10, APC, AD5-14H12, 130-113-314, Miltenyi) and XCR1 (1:10, PE, S15046E, 372604, BioLegend), and T cell phenotype was assessed by antibodies against CD25 (1:100 ...
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bioRxiv - Neuroscience 2020Quote: Free floating 30 μm sagittal brain sections were treated for antigen retrieval using sodium citrate buffer and subsequently stained using primary antibodies for Iba1 (1:250, rabbit, Wako) and anti-6E10 (1:1000, Biolegend), and the corresponding secondary antibodies and nuclear counterstain using DAPI ...
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bioRxiv - Cell Biology 2020Quote: ... All FACS antibodies were used at 1:100 dilution in the presence of TruStain fcX blocker (101320, Biolegend, 1:50). For PDGFRa immunofluorescence staining ...
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bioRxiv - Cancer Biology 2020Quote: ... and 200 μl of primary antibody (1:200 anti-αSMA antibody, eBioscience, clone 1A4, efluor 570; 1:200 anti-CD31 antibody, Biolegend, clone MEC13.3 ...
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bioRxiv - Cell Biology 2021Quote: ... 0.1% Sodium Azide and with one of the following primary antibody dilutions: Mouse Anti-HA (1:2000; HA.11, Biolegend), Mouse Anti-V5 (1:2000 ...
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bioRxiv - Cell Biology 2022Quote: ... The cells were then blocked (PBS-0.25% Triton X-100+ 1% BSA (bovine serum albumin)) for 1h at RT and then primary antibodies (anti-βIII tubulin (Tuj1, 1:1000, BioLegend), anti-NFH (1:1000 ...
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bioRxiv - Cell Biology 2022Quote: ... Stained cells and “fluorescence minus one” controls were then resuspended in SB + 1:1000 DNase + 1:1000 Draq7 (Biolegend, #424001) as a live/dead stain ...
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bioRxiv - Immunology 2022Quote: ... Bound virus was detected with rabbit polyclonal VP1 antibody (1:10000) followed by an anti-rabbit secondary (Biolegend, 1:200). Samples were acquired on an LSRFortessa flow cytometer (BD Biosciences ...
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bioRxiv - Molecular Biology 2021Quote: ... 1 U/ml erythropoietin) for 1 hour following disaggregation before being stained (anti-CD71 Brilliant Violet 421, 1:5000 dilution – 113813 Biolegend, RRID ...
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bioRxiv - Immunology 2020Quote: ... cells were incubated with K562 at a 1:1 E/T ratio with addition of a CD107a FITC mAb (H4A3, BioLegend) in a 96-round bottom plate with a centrifugation step of 5min at 500rpm ...
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bioRxiv - Molecular Biology 2022Quote: ... 1 mg of protein (1/10 of input transferred in a new tube) was incubated together with 1 μL of anti-HA antibodies (BioLegend 901502 anti-HA.11 clone 16B12 ...
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bioRxiv - Cancer Biology 2022Quote: ... 5mM EDTA and 1% BSA) and incubated with the following primary antibodies: anti-mouse CD45 (Brilliant Violet 570, 30-F11, 1:200, Biolegend), anti-mouse Fce-R1 alpha (PE-Cyanine7 ...
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bioRxiv - Immunology 2020Quote: ... and other epithelial cells markers (anti-mouse CD326 Brilliant Violet 605, 1:200 Biolegend 118227 and PerCP/Cy5.5 anti-mouse CD24, 1:200 Biolegend 101824) for 20 minutes at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... for sorting or CD34-eFluor 660 (eBioscience, clone RAM34, 1:100) and ITGA6-pacific blue (Biolegend, clone GoH3, 1:200) for analysis ...
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bioRxiv - Immunology 2020Quote: ... Cells were stained with the tetramer plus CD3-eFluor 450 (1/100, 48-0032-82 eBioscience) and CD8-FITC (1/100, 300905 BioLegend) at 4°C for 30 min ...
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bioRxiv - Immunology 2021Quote: ... Heparinized blood diluted 1:1 in PBS overlaid onto Lymphopure density gradient medium 1.077 g/mL (BioLegend, San Diego, USA) in Leucosep tubes (Greiner Bio-One ...
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bioRxiv - Immunology 2021Quote: ... Candidate TCR-transduced T cells were then added in at an E:T ratio of 1:1 for 6h in the presence of anti-CD107A-BV650 (Clone H4A3, Biolegend 328638) and Golgi Block ...
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bioRxiv - Immunology 2020Quote: ... total thymocytes were activated with 1 μg/mL plate bound anti-CD3 and soluble 1 μg/mL anti-CD28 in the presence of monensin (BioLegend). After 4hrs ...
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bioRxiv - Immunology 2020Quote: ... Cells were then stained with anti-CD3-FITC (clone 145-2C11; 1:100; eBioscience) and anti-TCRδ (clone GL3; 1:100; Biolegend) for 30 min on ice ...
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bioRxiv - Immunology 2023Quote: ... Wells were then incubated with 100 μl HRP-conjugated mouse anti-His antibody (1:2000 dilution in 1% BSA/TBST, BioLegend).
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bioRxiv - Neuroscience 2023Quote: ... were used to confirm results with McSA1: a mouse monoclonal antibody to Aβ residues 1-16 (1:1000; clone 6E10; Cat#803001, Biolegend) or a mouse monoclonal antibody to Aβ residues 17-24 (1:1000 ...
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bioRxiv - Synthetic Biology 2023Quote: ... and then incubated with the primary antibodies α-FLAG polyclonal mouse (1:12,000) and α-RpoA monoclonal mouse (1:1,000) (Biolegend). Then ...
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bioRxiv - Physiology 2023Quote: ... Samples were next stained with surface marker antibodies (CD45-AF700 1:500 103128 Biolegend, CD31-Pacific Blue 1:500 102421 Biolegend, TER119-BUV496 1:500 741079 BD ...
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bioRxiv - Immunology 2023Quote: ... CXCR5-PE-Dazzle 594 (J252D4, 1:40), and CD38-PE-Cy7 (HIT2, 1:200) for GC B cells (all BioLegend). For donors 321-07 and 321-08 the PBMC samples for days 0 and 5 were stained with TotalSeq-C anti-human hashtag oligos 9 and 10 ...
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bioRxiv - Immunology 2023Quote: ... and stained for 30 min at 25 °C with BCL6-PE (7D1, 1:50) and Ki-67-PE-Cy7 (Ki-67, 1:400) (both BioLegend). Cells were washed twice with permeabilization/wash buffer and resuspended in P2 for acquisition on an Aurora using SpectroFlo v2.2 (Cytek) ...
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bioRxiv - Immunology 2023Quote: ... Each sample was labelled with one specific hashtag (1:400 dilution) and CITE-seq antibodies (1:800 dilution, all Biolegend). After labelling for 30 min at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... Stained T-IEL and unstained lymph node T cells were mixed at a 1:1 ratio before stimulation with 30 μg/ml anti-CD3 (Clone 145-2C11, Biolegend) crosslinked with 5 μg/ml anti-Hamster (Jackson Immunoresearch) ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies, rabbit anti-phospho-cofilin (CST, #3311, 1:100) and mouse anti-SMI-32 (BioLegend, # SMI-32R, 1:250), were applied in blocking buffer at 4°C overnight ...
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bioRxiv - Microbiology 2022Quote: ... using 1:1000 of polyclonal rabbit anti-human MCT1 IgG (Cell Signalling) and 1:1000 mouse anti-human CD147 IgG (Biolegend) followed by HRP-conjugated secondary IgG antibody of specific host type ...
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bioRxiv - Cell Biology 2023Quote: ... Cell surface expression level of syndecan-1 was evaluated by incubating cells with anti-syndecan-1 antibody (10 µg/ml, B280312, Biolegend) for 1h at 4 °C ...
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bioRxiv - Immunology 2023Quote: ... along with co-stimulatory antibodies (CD28 and CD49d at 1 μL/mL) and Brefeldin-A (1 μL/mL, BioLegend, USA). LIVE/DEAD and surface staining was performed as described above ...
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bioRxiv - Neuroscience 2023Quote: ... the following antibodies were added in a 10 µl master mix: CD11b-FITC (M1/70, 1:400, 1:400, Biolegend), CD45-APC/Cy7 (30F11 ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were washed once with PBS and incubated in 50 µL PBS containing human Fc block (Supplementary Table 1) and Zombie Aqua viability dye (1:200, BioLegend) for 15 min at 4°C ...
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bioRxiv - Cell Biology 2023Quote: ... and NMHC-IIB antibody (#909901) binding to residues 1965-1976 in the C terminus (1:100 IF, 1:400 WB) were purchased from BioLegend. Rabbit polyclonal antibody against GFP tag (#50430-2-AP ...
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bioRxiv - Cancer Biology 2023Quote: ... 5 x105 cells were labeled with 1 μg anti-hCD81-PE clone 5A6 or 1 µg isotype control-PE (349506, 400114; Biolegend)32 and by Western blotting (4-12% SDS-PAGE ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed with FACs buffer (1× PBS, 1% FBS) and fixed at RT for 20 min with Intracellular Staining Fixation Buffer (BioLegend). Cells were washed with 2× Cyto-FastTM Perm Wash solution (CFPWS ...
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bioRxiv - Immunology 2024Quote: ... CD4 conv T cells were resuspended in complete RPMI 10% FBS and incubated for 1 hour at 37C with 1 ug/ml of anti-mouse CD3 (2c11 clone; Biolegend) or left untreated ...
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bioRxiv - Cancer Biology 2022Quote: ... and 1×PBS) mixed with TruStain FcX™ (anti-mouse CD16/32) Antibody (1:200, Clone: 93, BioLegend, CA, USA) and eBioscience™ Fixable Viability Dye eFluor™ 780 (1:1000 ...
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Convection and extracellular matrix binding control interstitial transport of extracellular vesiclesbioRxiv - Bioengineering 2022Quote: ... Fluorescently conjugated integrin antibodies (1:400 AF488 anti-integrin β1, clone-TS2/16, BioLegend; 1:200 PE anti-integrin β1, clone-TS2/16, BioLegend; 1:400 AF647 anti-integrin β1 ...
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bioRxiv - Developmental Biology 2023Quote: ... DNA and actin were stained with 1 μg/mL DAPI and phalloidin (1:1000, Flash Phalloidin™ Red 594; BioLegend). Testis were washed three times with PBS and mounted in (ProLong™ Gold Antifade Mountant ...
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bioRxiv - Cell Biology 2023Quote: ... Single cells were pelleted and re-suspended in a staining solution of DMEM containing 1% FBS and anti-Ki-67 Alex Fluor 647 (1:250 dilution in 3% BSA, Cat. No. 652407; BioLegend) for 1 hour on ice ...
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bioRxiv - Immunology 2024Quote: ... Apoptosis analysis was done with the APC Annexin V (1:50) apoptosis detection kit with PI (1:200) in Annexin V Binding Buffer (Biolegend).
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bioRxiv - Immunology 2024Quote: ... clone GK1.5-7; WEHI Antibody Facility); anti-IgD (1:200; clone 11-26c; eBioscience) and anti-GL7 (1:100; clone GL7; Biolegend).
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bioRxiv - Immunology 2024Quote: ... The pelleted cells were resuspended in FACS buffer (PBS + 1% bovine serum albumin + 2 mM EDTA + 0.1% sodium azide) containing 1:100 dilution of Fc Block and a 1:250 dilution of Zombie Violet dye (423113; Biolegend). After incubation at 4°C for 15 minutes ...
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bioRxiv - Bioengineering 2024Quote: ... 100 μL of staining solution containing APC SIINFEKL tetramer (1:600 dilution) and fluorescein isothiocyanate (FITC) CD8 antibody (1:100 dilution, Biolegend) was added to each tube in the ice bath and incubated for 30 minutes in the dark ...
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bioRxiv - Immunology 2024Quote: ... cells were plated at a density of 1×105 per mL of media and stimulated with anti-CD40 (1 μg/mL, clone 1C10, Biolegend), rmIL-4 (10 ng/mL ...
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bioRxiv - Immunology 2024Quote: ... before staining for 1 hour at room temperature with 250μL anti-TCRβ AF647 (H57-597, Biolegend, 1:100 in PBS). Cells were then washed three times in PBS ...
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bioRxiv - Immunology 2024Quote: ... the samples were biofunctionalized with antibodies by 90 min incubation in a 1:1 mix of biotinylated anti-human CD3 (OKT3 clone) and anti-human CD28 (both from Biolegend) at a concentration of 2 μg/mL in PSB with 2% BSA ...