4 2 Hydroxy 1 Methoxyethyl 1 2 Benzenediol citations for BioLegend

Quote: ... Incubation with primary antibodies (rat anti-human α-synuclein, 15G7, 1:100 Enzo Life Sciences; mouse anti-α-synuclein [Syn1, clone 4D6], 1:1000, 834301, BioLegend; rabbit anti-GAPDH 1:2000 (D16H11) Cell Signaling Technologies ...

The Olfm4-defined human neutrophil subsets differ in proteomic profile in septic shock

Hans Lundquist, et al., bioRxiv - Immunology 2022

Quote: ... 2×107 cells resuspended in 0.5 ml PBS were incubated with PE-conjugated mouse anti-human CD15 antibody (BioLegend, San Diego, CA, USA) diluted 1:40 ...

Nasal prevention of SARS-CoV-2 infection by intranasal influenza-based boost vaccination

Runhong Zhou, et al., bioRxiv - Immunology 2021

Quote: ... cells were washed with staining buffer (PBS containing 2% FBS) and surface stained with anti-mouse-CD4-PerCP/Cy5.5 (Biolegend Cat# 116012, RRID: AB_2563023), anti-mouse-CD8-BV785 (Biolegend Cat# 100750 ...

A comparative analysis of SARS-CoV-2 antivirals in human airway models characterizes 3CL^pro inhibitor PF-00835231 as a potential new treatment for COVID-19

Maren de Vries, et al., bioRxiv - Microbiology 2021

Quote: ... approximately 200,000 cells per sample were resuspended in staining buffer (PBS, 2% BSA, 0.01% Tween) and incubated for 10 minutes with Fc block (TruStain FcX, Biolegend; FcR blocking reagent, Miltenyi). Cells were then incubated with barcoded hashing antibodies for 30 min at 4 °C ...

G307S DNAM-1 mutation exacerbates autoimmune encephalomyelitis via enhancing CD4⁺ T cell activation

Rikito Murata, et al., bioRxiv - Immunology 2022

Quote: ... Sorted naïve 2D2 CD4+ T cells were activated with plate-coated 2 μg/mL anti-mouse CD3e mAb (clone 145-2C11, BioLegend, San Diego, CA) and soluble 1 μg/mL anti-mouse CD28 mAb (clone 37.51 ...

Exploring heteroaromatic rings as a replacement for the labile amide of antiplasmodial pantothenamides

Jinming Guan, et al., bioRxiv - Pharmacology and Toxicology 2020

Quote: ... 1 μg/mL anti-CD71 APC (clone R17217) (eBioscience, San Diego, CA) and 2 μg/mL anti-TER119 PE-Cyanine7 (BioLegend, San Diego, CA). The cells were stained on ice for 30 min ...

Replication-dead gammaherpesvirus vaccine protects against acute replication, reactivation from latency, and lethal challenge in mice

Wesley A. Bland, et al., bioRxiv - Microbiology 2023

Quote: 2 × 106 single cell suspensions prepared from the lung or spleen were resuspended in 200 ul of PBS with 2% fetal bovine serum and blocked with TruStain fcX (BioLegend, San Diego, CA). T cell subsets were identified with antibodies against CD45 (clone 30-F11 ...

Development of a Robust Consensus Modeling Approach for Identifying Cellular and Media Metabolites Predictive of Mesenchymal Stromal Cell Potency

Alexandria Van Grouw, et al., bioRxiv - Bioengineering 2023

Quote: ... PBMCs were collected and stained using Brilliant Violet 711 anti-CD4 and APC anti-CD8 (Supporting Information Table 2) (Biolegend, San Diego CA). PBMCs were first washed and stained with Zombie Yellow (Supporting Information Table 2 ...

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