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Citations for BioLegend :
3001 - 3050 of 8901 citations for DNAM 1 Human HEK 293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2022Quote: ... and of soluble triggering receptor expressed on myeloid cells 2 (sTREM2) and brain-derived neurotrophic factor (BDNF) were assessed using the Human LEGENDplex Multiplex Assay (BioLegend®) was used following manufacturer’s instructions (Lehmann et al ...
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bioRxiv - Immunology 2022Quote: ... CD8 T cells were stimulated in presence of self-made human IL-2 on α-CD3 (5 µg/ml) (145-2C11, BioLegend), α-CD28 (5 µg/ml ...
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bioRxiv - Genetics 2022Quote: ... Differentiation efficiency was measured using Fluorescence Activated Cell Sorting (FACS) following staining with an APC conjugated antibody to HLAG (APC anti-human HLA-G Antibody Clone: 87G, BioLegend UK), in unmodified hTSC this was consistently between 40-70% ...
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bioRxiv - Microbiology 2022Quote: The level of TNF-α protein expression was evaluated after cell transduction with TPA-TNFα in the cultured medium using human TNFα ELISA kit (Biolegend, UK). First ...
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bioRxiv - Neuroscience 2024Quote: ... PE/Cyanine7 anti-mouse/human CD45R/B220 Antibody (103222) and APC anti-mouse CD3 Antibody (100236) antibodies (Biolegend, San Diego, CA) were utilized for the lymphoid populations ...
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bioRxiv - Immunology 2024Quote: ... Cells were washed once with PBS supplemented with 1mM EDTA and resuspend in FcR blocking buffer using Human TruStain FcX (Biolegend, 422302) and Mouse TruStain FcX PLUS (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... Cultured Raji cells and MV-4-11 cells were washed twice with PBS and stained with FITC-conjugated mouse anti-human CD19 (Biolegend, 302205) for 30 mins ...
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bioRxiv - Cancer Biology 2023Quote: ... Wells were washed 3 times before adding 50 µL per well of Biotin anti-human IFN-γ Antibody (clone 4S.B3; #502504, BioLegend, USA) diluted 1:400 in 1% SureBlock solution and incubated 1 h at RT ...
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bioRxiv - Bioengineering 2023Quote: ... Successful blocking was confirmed by staining with anti-human CD44 antibody conjugated with Alexa Fluor 594 (clone: C44 Mab-5; Biolegend, USA) and quantified using the Cytek Aurora flow cytometer (4L 16V-14B-10YG-8R ...
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bioRxiv - Bioengineering 2023Quote: ... Cells were then stained for transferrin receptor expression by adding 0.25 μg of PE/Cy7 anti-human CD71 [CY1G4] (Biolegend, Cat# 334111) directly to the blocking solution for 30 min at room temperature ...
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bioRxiv - Cancer Biology 2023Quote: The antibody mix for CTC detection of human CTCs in animal models contained a rat anti-mouse CD45 (30-F11) antibody conjugated to PE (Biolegend; 103106) and mouse anti-human HLA conjugated to AlexaFluor 647 (Biolegend ...
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bioRxiv - Cancer Biology 2022Quote: ... LEPs from co-cultures were separated after 10 days for gene expression qPCR analysis by FACS using anti-human CD133-PE (BioLegend, clone7) and anti-human CD271-APC (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... K562 cells were settled with vehicle-treated or Verbenalin-treated NK cells in the presence of Brilliant Violet 421™ anti-human CD107a (LAMP1) antibody (Biolegend) and GolgiStop™ (BD Biosciences) ...
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bioRxiv - Microbiology 2023Quote: ... opsonized bacteria were re-suspended in sterile PBS with 2% fetal bovine serum and stained with APC-conjugated anti-human C3b (Biolegend 846106) and FITC-conjugated anti-human C5b-C9 (LSBio C210266 ...
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bioRxiv - Bioengineering 2023Quote: Cytokines from undiluted samples from chips/plates were analyzed by fluorescent bead-based multiplex sandwich immunoassays (Legendplex™ Human CD8/NK Mix and Match Subpanel; BioLegend) containing capture beads targeting IL-2 ...
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bioRxiv - Immunology 2023Quote: Cytokine levels in the supernatants from B cells that had been stimulated for 48 hr were measured using the LEGENDplex™ Human B cell Panel (13-plex) (Biolegend), using the same procedure as described for the antibody measurements ...
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bioRxiv - Immunology 2023Quote: Antibody levels in the supernatants from B cells that had been stimulated for 6 days were measured using the LEGENDplex™ Human Immunoglobulin Isotyping Panel (6-plex) (Biolegend), a bead-base immunoassay ...
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bioRxiv - Cell Biology 2023Quote: ... Remaining chemokine levels in the supernatants of cultured cells were measured in triplicate using the commercially available colorimetric LEGEND MAXTM human CCL5 ELISA kit (BioLegend, 440807) according to the manufacturer’s instructions and read with a SpectraMax M5 plate reader (Molecular Devices ...
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bioRxiv - Genetics 2023Quote: ... and 72 h by immunofluorescent staining with a PE-conjugated anti-human CD41/CD61 primary antibody (BioLegend, San Diego, CA, USA) using flow cytometric analysis.
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bioRxiv - Immunology 2023Quote: ... 1x106 B cells in 100 μl buffer were incubated with a panel of antibodies including 3 μl FITC anti-human CD20 antibody (BioLegend, 302304), 3.5 μl Brilliant Violet 421 anti-human CD27 antibody (BioLegend ...
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bioRxiv - Cell Biology 2023Quote: ... to assess viable cells and fluorophore-conjugated monoclonal antibodies to the following human cell-surface markers: anti-CD45-BV650 (Biolegend, 304044), anti-CD206-AF647 (Biolegend ...
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bioRxiv - Cell Biology 2023Quote: Lymph nodes were digested as described above and enriched for CD45- stromal cells by negative selection using MojoSort™ Human CD45 Nanobeads (Biolegend). CD45- cell enrichment was performed according to manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: The antibodies used for flow staining in this study are: rat anti-mouse/human CD11b conjugated to BV605 (clone M1/70, Biolegend, 101257), hamster anti-mouse CD3e conjugated to BV510 (clone 145-2C11 ...
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bioRxiv - Cell Biology 2024Quote: ... The secretion levels of IL-1β and TGF-β were quantified using enzyme-linked immunosorbent assay (ELISA) by human IL-1β ELISA Max Deluxe kit (437004, BioLegend) and human TGF-β1 duoset ELISA kit (Dy204-05 ...
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bioRxiv - Developmental Biology 2024Quote: ... Dissociated cells were first incubated with 5uL of FcR blocker for 5 min at room temperature and stained with anti-CD45 antibody (BV785 anti-human CD45 antibody, BioLegend, 304048) and DAPI (Sigma-Aldrich ...
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bioRxiv - Bioengineering 2024Quote: ... CD2 target gene activation was quantified by cell surface antibody staining of live cells using APC anti-human CD2 antibody (Biolegend, 309224) followed by flow cytometry on the cell population transfected with both gRNA and dCasMINI plasmids (performed on Cytoflex and analyzed using Flowjo software) ...
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bioRxiv - Cancer Biology 2024Quote: ... Stimulated PBMCs were maintained in RPMI-1640 supplemented media with 100 ng/ml CD3 and 10 ng/ml recombinant human IL-2 (Biolegend, 589104).
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bioRxiv - Cancer Biology 2024Quote: Output of CD34+ cells following hematopoietic differentiation of hiPSCs was determined by staining with PE/Dazzle594-labeled anti-human CD34 antibodies (BioLegend, #343534). Prior to addition of antibodies ...
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Cellular sialoglycans are differentially required for endosomal and cell-surface entry of SARS-CoV-2bioRxiv - Microbiology 2024Quote: ... washed cells were resuspended in 100 μL of FACS buffer with 5 μL of PE anti-human TMPRSS2 antibody (BioLegend #378403) per million cells and incubated for 30 min at 4 °C in the dark ...
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bioRxiv - Immunology 2024Quote: ... These cells were stained with a cocktail of mAb conjugated with DNA-barcoded oligonucleotide that are specific for immune cell lineages (TotalSeq™-C Human TBNK Cocktail, BioLegend) according to the manufacturer’s protocol ...
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bioRxiv - Developmental Biology 2024Quote: ... Dissociated cells were first incubated with 5uL of FcR blocker for 5 min at room temperature and stained with anti-CD45 antibody (BV785 anti-human CD45 antibody, BioLegend, 304048) and DAPI (Sigma-Aldrich ...
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bioRxiv - Developmental Biology 2021Quote: ... Cd49b-AF488 (Biolegend, clone HMα2, 1 µg ml−1, 1:500) and Sca1-AF647 (Biolegend ...
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bioRxiv - Immunology 2020Quote: ... flotillin-1 (W16108A; 1:1000; Biolegend), peIF2a (D9G8 ...
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bioRxiv - Cell Biology 2021Quote: ... Sca-1 (BioLegend, # 108126; 1:200), Ter119 (Thermo Fisher ...
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bioRxiv - Cell Biology 2021Quote: ... Mac-1 (Biolegend, 101226, 1:400), B220 (Biolegend ...
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bioRxiv - Cell Biology 2022Quote: ... CD48 (HM48-1; Biolegend; 1:100), CXCR4 (2B11 ...
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bioRxiv - Cell Biology 2020Quote: ... -CD48 (HM48-1, Biolegend, 1:100), -CD150 (TC15-12F12.2 ...
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bioRxiv - Cell Biology 2021Quote: ... 50 ng·ml−1 mCSF-1 (BioLegend), and 5 μg·ml−1 plasmocin (Invivogen ...
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bioRxiv - Developmental Biology 2023Quote: β1-Alexa647 (1:500, BioLegend, 102214)
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bioRxiv - Developmental Biology 2021Quote: ... and Sca1-AF647 (Biolegend, clone D7, 1 µg ml−1, 1:500). Cells were then stained with streptavidin-APC or Streptavidin-PE/Cy7 (BD-Biosciences ...
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bioRxiv - Cancer Biology 2023Quote: ... CD49f-PE/Cy7 (Biolegend, clone GoH3, 1 µg ml−1, 1:200). DAPI was used to detect dead cells ...
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bioRxiv - Immunology 2021Quote: ... the supernatants were collected to determine the levels of antibody productions (e.g., IgG1, IgG2, IgG3, IgG4, IgA, and IgM) by using LEGENDplex™ Human Immunoglobulin Isotyping Panel (Biolegend, CA, USA). The Gallios Flow Cytometer was utilized to analyze the data according to the manufacturer’s recommended protocol.
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bioRxiv - Immunology 2021Quote: Fluorophore- or biotin-conjugated antibodies specific for human cell-surface antigens and cytokines were as follows: anti-CD71 (CY1G4, BioLegend, DF1513, NovusBio), anti-CD235a (HI264 ...
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bioRxiv - Microbiology 2022Quote: ... Quantification of cytokine and chemokine levels in culture media was performed using the LEGENDPlex kit from BioLegend (human anti-virus response panel), according to the manufacturer’s recommendations.
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bioRxiv - Genomics 2020Quote: ... The cells were centrifuged at 300xg for 5 minutes at room temperature and resuspended in 85 ul of staining buffer and incubated with 5 ul of Human TruStain FcX(Biolegend cat: 422301) for 10 minutes on ice in 5 ml FACS tubes ...
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bioRxiv - Immunology 2021Quote: For bead-based isolation of human CCR5+ and CCR5− cPMN were sorted using anti-APC MojoSort™ nanobeads (Biolegend, San Diego, USA) according to the manufacturer’s recommendations ...
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bioRxiv - Cell Biology 2022Quote: ... of the sorted MBS-MEF and MCF7 cell populations was then counterstained with Alexa-488-labeled anti-human CD326 (Biolegend; Cat #: 324210) and PE-labeled anti-mouse CD321 antibody (BD Biosciences ...
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bioRxiv - Cancer Biology 2020Quote: ... Cell surface marker expression in the SORE6+ and SORE6-compartments of MDA-MB-231 cells cultured in vitro or recovered from primary tumors was assessed by flow cytometry following staining with Brilliant Violet 421TM anti-human CD133 (Biolegend Cat#372807), or APC anti-human CD44 (Biolegend Cat#338805 ...
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Inhibition of major histocompatibility complex-I antigen presentation by sarbecovirus ORF7a proteinsbioRxiv - Microbiology 2022Quote: ... with 1 × 106 cells/mL 5000 rad irradiated PBMC + 50 U/mL IL-2 + 10 ng/mL IL-15 (both from NCI BRB Preclinical Biologics Repository) + 0.1 μg/mL each of anti-CD3 (Ultra-LEAF purified anti-human CD3 antibody clone OKT3; BioLegend, San Diego, CA) and anti-CD28 (Ultra-LEAF purified Anti-human CD28 antibody clone 28.2 ...
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bioRxiv - Immunology 2022Quote: ... Supernatants were then evaluated for IFN-γ levels using the Legend Max ELISA kit with pre-coated plates (human IFN-γ) (Biolegend). Surface phenotype of cells that had been removed from the well was determined by flow cytometry using fluorochrome conjugated antibodies specific for 4-1BB or CD69.