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Citations for BioLegend :
2951 - 3000 of 8901 citations for DNAM 1 Human HEK 293 Fc since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Genomics 2023Quote: ... The cells were then incubated on ice for another 30 min after adding 0.5 μl of biotin anti-human CD15 antibody (BioLegend, 301913). After immunostaining ...
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bioRxiv - Genomics 2023Quote: ... the cells were resuspended in 100 μl chilled DPBS with 0.2% BSA and 10 μl of human TrueStain FcX (BioLegend, 422302) and incubated on ice for 10 min to reduce non-specific labeling ...
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bioRxiv - Immunology 2023Quote: ... cells were first resuspended in cell staining media (CSM) (Fluidigm, South San Francisco, CA, USA) with 5ml Human TruStain FcXTM block (Biolegend) for 5 min at room temperature to block Fc receptors ...
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bioRxiv - Immunology 2023Quote: ... then Fc gamma receptors were blocked for 15 minutes with heat-inactivated human serum prepared in-house or anti-mouse CD16/32 (TruStain FcX, Biolegend) for human samples and mouse samples respectively ...
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bioRxiv - Cancer Biology 2023Quote: ... Antibodies used to distinguish the different cell populations included Brilliant violet 421 anti-human CD19 (Biolegend, San Diego, CA, USA), PE anti-human CD45 (Biolegend) ...
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bioRxiv - Cancer Biology 2023Quote: ... 100 000-200 000 cells per sample were processed by blocking with 5 µl of Human TruStain FcX Blocking Solution (BioLegend) for 10 min at 4°C ...
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bioRxiv - Biochemistry 2024Quote: ... following an overnight incubation at 4°C with the primary antibody against residues 103-108 of human α-synuclein (BioLegend, 807801 [4B12] 1:1000 ...
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bioRxiv - Systems Biology 2024Quote: ... Ultra-LEAF anti-human HLA-A,B,C antibody (clone W6/32, cat#: 311448) for immunopeptidomics was purchased from BioLegend. Polyethylenimine (PEI ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were resuspended in FACS buffer (PBS plus 2% FBS) and blocked with monoclonal antibody to CD16/32 (Human TruStain FcX, #422302, Biolegend) for 10 min at 4°C before staining with surface antibodies ...
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bioRxiv - Cancer Biology 2023Quote: ... and cell surface proteins were stained with the following antibodies (5 μl per test in 100 μl flow buffer) at 4 °C for 20 minutes: FITC anti-human CD45 (368508; BioLegend), FITC anti-human CD14 (325604 ...
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bioRxiv - Molecular Biology 2023Quote: ... The percentage of purified CD34+ cell was analysed by flow cytometry staining with anti-human CD34 PE antibody (BioLegend, UK). The cells were cultured in StemSpan SFEM II medium (StemCell Technologies ...
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bioRxiv - Immunology 2024Quote: ... cells from each well were washed thrice with PBS and stained with APC/Cyanine7 anti-human CD14 Antibody (BioLegend, #123317) to assess THP-1 cell phagocytosis of HER2-positive tumor cells ...
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bioRxiv - Developmental Biology 2024Quote: ... For surface antigen staining cells were incubated for 20 min with APC-labelled anti-human CD56 (Biolegend, clone TS2/16) and PE-labelled anti-human CD82 (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... or intracellular staining for APC-conjugated anti-human IL-17A (17-7179-42, eBioscience, USA) and Foxp3 (320014, BioLegend, USA) was performed according to the manufacturer’s instructions.
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bioRxiv - Immunology 2024Quote: ... cells were incubated for 25 minutes at 4°C with the following antibodies: BV711 anti-mouse/human CD11b (Cat# 101242; BioLegend), PE anti-mouse CX3CR1 (Cat# FAB5825P ...
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bioRxiv - Bioengineering 2024Quote: The following anti-human antibodies were used in the Jurkat staining panel: anti-CD4 FITC (BioLegend catalog #317408, clone OKT4), anti-CD25 PerCP/Cyanine (Cy ...
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bioRxiv - Cell Biology 2024Quote: ... IL-8 concentration in medium samples was measured by using the ELISA MAX Deluxe Set Human IL-8 (BioLegend, 431504) according to the user manual.
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bioRxiv - Immunology 2024Quote: ... and 5 µg/mL of soluble Ultra-LEAF™ purified anti-human CD28 monoclonal antibody (clone 28.2, BioLegend, cat. 302933) in R10 medium [RPMI-1640 medium supplemented with 10% heat-inactivated FBS ...
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bioRxiv - Neuroscience 2019Quote: ... 1-16 (1:1000, Biolegend). Sections were viewed ...
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bioRxiv - Cell Biology 2020Quote: ... Sca-1 (1:200; BioLegend), and Ter119 (1:200 ...
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bioRxiv - Cancer Biology 2024Quote: ... PD-1 (BioLegend, 1:100), phopho-STING (ThermoFisher 1:100) ...
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bioRxiv - Biophysics 2021Quote: ... This step was performed in order to break the existing cadherin junctions and allow the blocking antibodies (anti N-Cadherin antibody: LEAF™ Purified anti-human CD325, #350804, Biolegend, USA ...
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bioRxiv - Immunology 2020Quote: ... Blood CD4+ T cells were isolated from healthy donor or patient PBMCs using a human CD4+ T cell isolation kit (BioLegend #480010). Purified CD4+ T cells were used without stimulation ...
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bioRxiv - Cell Biology 2022Quote: ... Human cell subpopulations were detected in the bone marrow of transplanted mice using the following antibodies: anti-human CD45-APC (Biolegend, 368512), anti-human CD3-Pacific Blue (Biolegend ...
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bioRxiv - Molecular Biology 2021Quote: ... cells were first blocked with 5% BSA for 30 min and stained with Alexa Fluor® 647 anti-human CD161 antibody (clone HP-3G10; BioLegend) at 10 μg/ml in blocking solution for 60 min ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were then incubated with APC-conjugated anti-human HLA antibody (clone W6-32; cat. no. 311410; BioLegend, San Diego, CA). Cells were subsequently analyzed by flow cytometry for GFP cells/DsRed cells (sensitive/resistant ...
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BRD2 inhibition blocks SARS-CoV-2 infection by reducing transcription of the host cell receptor ACE2bioRxiv - Cell Biology 2021Quote: ... CD81 expression was measured 7 days post-transduction by dislodging cells with TrypLE and live cells were stained with APC-conjugated anti-human CD81 antibody (Biolegend 349509). CD81 expression was assessed on a BD LSRII with >90% of Calu3-CRISPRi cells with CD81 knocked down compared to a non-targeting sgRNA control.
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bioRxiv - Cancer Biology 2022Quote: ... and Zombie Dye Violet Blue (Biolgend; 423114) for 15 minutes at 4degC followed by surface labeling with PE anti-human CD45 (Biolegend; 304008) for 30 minutes at 4degC ...
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bioRxiv - Immunology 2022Quote: ... the plate was washed five times with PBST and incubated with anti-mouse secondary antibody with minimal cross reactivity with human IgG (Biolegend, 405306). The plate was washed five times after 1-hour secondary antibody incubation and developed with tetramethylbenzidine substrate (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... Every 10^6 B cells in 100 μL solution were then stained with 3 μL FITC anti-human CD20 antibody (BioLegend, 302304), 3.5 μL Brilliant Violet 421 anti-human CD27 antibody (BioLegend ...
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bioRxiv - Immunology 2022Quote: ... 0.1% BSA/PBS 15 min on ice) and incubated with either FITC-anti-human CD3 (clone HIT3a; BioLegend, San Diego, CA) and PE-anti-human CD56/NCAM (HCD56 ...
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bioRxiv - Microbiology 2020Quote: ... 5 × 105 cells/mL of CD14+ enriched cells were stimulated using recombinant human (rh) GM-CSF and rhIL-4 (BioLegend, USA) at a concentration of each 500 IU/mL in RPMI1640 media supplemented with 10% FCS at 37°C in 5% CO2 ...
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bioRxiv - Microbiology 2020Quote: ... Annexin V-APC (e-biosciences, San Diego, CA) and with cell surface markers α-human CD4 PE (Biolegend, San Diego, CA), α-human CD3 PerCP (BD Biosciences ...
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bioRxiv - Microbiology 2020Quote: ... The levels of murine IL-6 and TNF-α and human IL-6 were measured using ELISA MAX™ kits (BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Cell Biology 2022Quote: The cytokines in adipocytes supernatant were quantified by a bead-based multiplex assay (LEGENDplexTM Human Panel; BioLegend, San Diego, CA, USA), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2022Quote: The concentration of IFN-γ was determined in the supernatant of 6×106 cells from each donor (6 healthy donors) using the Human IFN-γ ELISA deluxe set (BioLegend) according to the manufacturer’s protocol.
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bioRxiv - Immunology 2019Quote: ... The following ELISA kits were used according to manufacturer recommendations: ELISA Max human ELISA for IL-10 (Biolegend, San Diego, CA), anti-pan human IFN alpha (Interferon Source ...
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bioRxiv - Cancer Biology 2020Quote: ... Cells were harvested for 5 min at 400g at RT and stained with Pacific Blue™ anti-human Ki-67 Antibody (Biolegend) according to eBioscience(™ ...
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bioRxiv - Microbiology 2021Quote: ... CD4+ T cells from blood were isolated from healthy donor peripheral blood mononuclear cells (PBMCs) using the BioLegend human CD4+ T cell isolation kit (BioLegend #480010). Purified CD4+ T cells were co-stimulated with plate-bound CD3 antibody (Biolegend #300333 ...
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bioRxiv - Immunology 2021Quote: ... according to the manufacturer’s instructions and naïve CD8+ cells were obtained using the MojoSort Human CD8+ Naïve T Cell Isolation Kit (Biolegend, Cat no.480045). Purity was consistently over >75% CD8+ with no contamination of CD4+ T cells and >80% of CD8+ T cells being naive ...
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bioRxiv - Cancer Biology 2019Quote: ... Circulating leukemia cells were detected on day 8 by flow cytometry using a human-specific CD45 antibody (#404012, Biolegend, San Diego). Mice were sacrificed upon signs of morbidity.
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bioRxiv - Cancer Biology 2019Quote: ... TDEs in 100 μl final volume of PBS were stained using the PE anti-human CD9 antibody (Clone HI9a) (BioLegend, CA) and incubated for 1 h at 4°C ...
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bioRxiv - Cell Biology 2020Quote: ... The antibodies used for flow cytometry were as follows: PE-conjugated mouse anti-human CD29 (Biolegend, San Diego, CA, USA; 303004), PE-conjugated mouse anti-human CD73 (BD Biosciences ...
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bioRxiv - Immunology 2020Quote: ... in the presence or absence of human IFN-β (500 U/ml: Pestka Biomedical Laboratories) or IL-27 (100 ng/ml: BioLegend) without adding IL-2.
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bioRxiv - Genetics 2021Quote: ... A antibody panel was used to identify and sort the CD3+CD8+ T cell population: Human TrueStain FcX (Biolegend; Cat# 422302), PE anti-human CD45 (Biolegend ...
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bioRxiv - Immunology 2020Quote: The concentrations of cytokines and chemokines in MLs and fibroblasts were measured by using a LEGENDplex™ Human Adipokine Panel (740196; BioLegend) according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: 600 million cells from human tonsils were resuspended in Miltenyi magnetic-activated cell sorting (MACS) buffer and stained with biotinylated anti-human CD19 (clone: HIB19; BioLegend, #555411) on ice for 30 min ...
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bioRxiv - Immunology 2021Quote: Leukocytes from whole blood and ETA samples were incubated with oligo-conjugated Ig-A/D/G/M for 10 min at 4°C followed by the addition of Human TruStain FcX™ (BioLegend) and 10 min incubation at RT ...
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bioRxiv - Cell Biology 2021Quote: Analysis of CAR expression on transduced NK92 cells was done by staining with APC-conjugated anti-human CD314 (NKG2D) mAbs and anti-Flag Tag mAbs (Biolegend, USA). NKG2DLs expression in each cancer cell line was stained with recombinant human NKG2D Fc chimera (R&D Systems ...
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bioRxiv - Cancer Biology 2022Quote: ... FACS buffer recipe was D-PBS + 5% FBS + 2% BSA + 5 mM EDTA + 50 µg/ml DNase-I with Human Trustain (Biolegend, 422302). All the compensation was done using UltraComp eBeads™ Compensation Beads (Invitrogen ...