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Citations for BioLegend :
2951 - 3000 of 3701 citations for Marker 7 Pcb Mixture W Pcb 118 13C12 99% 100 Ng Ml In Nonane since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... washed FACS buffer and stained with a 1:100 dilution of anti-mF4/80 or anti-m/hCD11b antibody (Biolegend) FACS buffer for 30 min at 4 °C ...
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bioRxiv - Neuroscience 2023Quote: ... Primary antibodies, rabbit anti-phospho-cofilin (CST, #3311, 1:100) and mouse anti-SMI-32 (BioLegend, # SMI-32R, 1:250), were applied in blocking buffer at 4°C overnight ...
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Cancer-associated fibroblasts produce matrix-bound vesicles that influence endothelial cell functionbioRxiv - Cancer Biology 2023Quote: ... 106 cells were incubated with PE anti-CD31 ([WM59] 303105), PE anti-CD45 ([2D1] 368510) and PE anti-CD326 (EpCAM, [9C4] 324206) antibodies (1/100, BioLegend) and human TruStain FcXTM (1/200 ...
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bioRxiv - Immunology 2023Quote: ... Samples were then washed twice in 1X PW then fixed in 100 μl of FluoroFix buffer (BioLegend: San Diego, CA), washed twice with FACS ...
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bioRxiv - Genomics 2023Quote: ... the cells were resuspended in 100 μl chilled DPBS with 0.2% BSA and 10 μl of human TrueStain FcX (BioLegend, 422302) and incubated on ice for 10 min to reduce non-specific labeling ...
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bioRxiv - Genomics 2023Quote: ... and all the samples were treated with sybr green (1x) and mouse anti-human IgG conjugated with Alexflour 647 (1:100 dilution, from BioLegend) for 30 mins at room temperature ...
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bioRxiv - Immunology 2023Quote: ... Cells were then pelleted and resuspended in 100 µL of Zombie Green viability dye (1:500 in PBS; BioLegend, 423111). After 15 minutes on ice ...
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bioRxiv - Neuroscience 2023Quote: ... clone RM4.5), CD25 (1:500; eBioscience, clone PC61.5), ITGA2 (1:50; eBioscience, Clone DX5) and MCAM (1:100; Biolegend, Clone ME9F1) for 15 min at RT ...
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bioRxiv - Cell Biology 2023Quote: ... and NMHC-IIB antibody (#909901) binding to residues 1965-1976 in the C terminus (1:100 IF, 1:400 WB) were purchased from BioLegend. Rabbit polyclonal antibody against GFP tag (#50430-2-AP ...
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bioRxiv - Cancer Biology 2023Quote: ... and incubated for 30 minutes on ice with the following antibody: anti-CD44 APC/Fire 750 (1:100, Biolegend, 103061), anti-CD24 PE (1:20 ...
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bioRxiv - Cancer Biology 2023Quote: ... and cell surface proteins were stained with the following antibodies (5 μl per test in 100 μl flow buffer) at 4 °C for 20 minutes: FITC anti-human CD45 (368508; BioLegend), FITC anti-human CD14 (325604 ...
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bioRxiv - Immunology 2024Quote: ... The pelleted cells were resuspended in FACS buffer (PBS + 1% bovine serum albumin + 2 mM EDTA + 0.1% sodium azide) containing 1:100 dilution of Fc Block and a 1:250 dilution of Zombie Violet dye (423113; Biolegend). After incubation at 4°C for 15 minutes ...
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bioRxiv - Bioengineering 2024Quote: ... 100 μL of staining solution containing APC SIINFEKL tetramer (1:600 dilution) and fluorescein isothiocyanate (FITC) CD8 antibody (1:100 dilution, Biolegend) was added to each tube in the ice bath and incubated for 30 minutes in the dark ...
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bioRxiv - Immunology 2024Quote: ... in the presence of anti-Ly6G AF488 (1A8) and anti-CD68 AF647 (FA-11) or their isotypes (1/100 each, Biolegend), and counterstained using 500ng/mL DAPI dilactate (Invitrogen) ...
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bioRxiv - Immunology 2024Quote: ... LAD2 cells were washed and incubated with the reaction products or serum-purified C3 or C3a (100 nM) along with a PE-conjugated anti-LAMP1 antibody (H4A3, Biolegend) for 1 h at 37 °C ...
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bioRxiv - Immunology 2024Quote: ... The cell suspension was stained using Brilliant Violet 785™ anti-mouse CD45 antibody (1:100, Biolegend, Clone 30-F11), Brilliant Violet 421™ anti-mouse F4/80 antibody (1:100 ...
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bioRxiv - Immunology 2024Quote: ... Dissociated lung cells or peripheral blood (100 µL) was incubated 20-min in the dark with anti-CD45-allophycocyanin and anti-CD31-Pacific blue (Biolegend). Erythrocytes were lysed with Red Cell Lysis Buffer (Biolegend) ...
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bioRxiv - Immunology 2021Quote: ... with or without adding Gal-9 mAb (10 µg/mL, Biolegend, CA, USA).) ...
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bioRxiv - Molecular Biology 2020Quote: ... and cell pellets were resuspended in 0.5 ml of cell staining buffer (BioLegend) supplemented with 5 µl of 7AAD per million cells ...
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bioRxiv - Neuroscience 2022Quote: ... was added to differentiation media and 10µg/ml of control IgG (Biolegend 403501) was added to the differentiation and proliferation control samples ...
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bioRxiv - Neuroscience 2021Quote: ... Cell pellet was resuspended in cold 0.5 ml RBC lysis buffer (Biolegend, 420301), transferred to 15 ml falcon tubes and incubated for 30 sec ...
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bioRxiv - Microbiology 2020Quote: ... Secondary detection used biotin-conjugated anti-mouse IgG (BioLegend #405303, 0.5 μg/ml), followed by HRP-Streptavidin conjugates (BioLegend #405210 ...
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bioRxiv - Microbiology 2021Quote: ... in the presence of 2 μg/mL soluble αCD28 antibody(clone CD28.2, Biolegend). RNAi knockdown of DCAF1 was performed as described before52 using ON-TARGET plusHuman DCAF1 siRNA - SMARTpool (Dharmacon ...
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bioRxiv - Immunology 2022Quote: ... MEL624 was pre-treated with hIgG1-Fc (10ug/ml) (Biolegend, San Diego, CA) or Siglec-5-Fc chimeric protein (10ug/ml ...
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bioRxiv - Pathology 2022Quote: ... and incubated with anti-Ter119 (4µg/mL, BV421-conjugated, Biolegend, #116233, RRID: AB_10933426) in 1X Perm buffer (eBioscience™ Foxp3 / Transcription Factor Staining Buffer Set ...
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bioRxiv - Immunology 2022Quote: ... the sera were applied to 3 ug/ml goat anti-rat IgG (BioLegend), or 1% BSA (ThermoFisher ...
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bioRxiv - Microbiology 2022Quote: ... Cells were incubated with 1 μg mL-1 PE-anti HA.11 (BioLegend) at 4 °C with rocking for 1 h ...
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bioRxiv - Microbiology 2022Quote: ... containing 1 μg/ml of the antibodies anti-mouse EpCam (PE-Cy7, Biolegend), anti-mouse Podoplanin (PE ...
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bioRxiv - Immunology 2022Quote: ... CD8 T cells were either stimulated with 2.5ug/ml anti- CD3 (Biolegend #100202) and anti-CD28 antibodies (Biolegend #102115 ...
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bioRxiv - Bioengineering 2022Quote: ... and 50 μg/mL anti-IL-2 Alexa Fluor 488 (Biolegend, Catalog: 500314) in 50μL washing buffer per 6 μL nanovial volume at 37°C for 30 minutes protected from light ...
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bioRxiv - Immunology 2022Quote: Tissue sections were stained using 5µg/mL purified mouse anti-CD3 (UCHT1; BioLegend) and 4µg/mL purified hamster anti-CD31 (2H8 ...
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bioRxiv - Immunology 2022Quote: ... (106 cells/50 mL) with anti-CD45 PercpCy5.5 (Cat# 103132, Clone#30F11, BioLegend), anti-CD4 PE (Cat#100408 ...
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bioRxiv - Immunology 2020Quote: ... and cultured for 4 days +/- αIL10 antibody +/- isotype control (5μg/ml, both Biolegend). Assessment of the direct effect of IL-10 on human CD4 T cells was performed by plate bound αCD3/28 (1μg/ml ...
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bioRxiv - Cancer Biology 2020Quote: ... Pelleted cells were re-suspend in 5 ml RBC lysis buffer (BioLegend 420301) for 10 min in RT and washed with 20 ml MACS buffer ...
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bioRxiv - Microbiology 2021Quote: ... in the presence of 2μg/ml soluble anti-CD28 antibody (clone CD28.2, Biolegend). The CEM-A cell line was obtained through NIH AIDS Reagent Program ...
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bioRxiv - Immunology 2020Quote: ... 2 μg/ml rat anti-mouse CD40 antibody (BioLegend, San Diego, California, USA), 2 ng/ml interleukin 7 (IL7 ...
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bioRxiv - Immunology 2021Quote: ... with addition of a biotin anti-mouse CD25 antibody (0.5 μg/mL; BioLegend). Magnetically-enriched CD4+ T cells were cultured in (DMEM ...
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bioRxiv - Immunology 2020Quote: ... washed cells were incubated with 2μg/ml anti-16/32 (TruStain FcX, BioLegend) for 5-10min prior to antibody staining ...
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bioRxiv - Immunology 2020Quote: ... Cells were then incubated with 10 μg/mL anti-CD16/CD32 (2.4G2, BioLegend) on ice for 10 min to block non-specific antibody binding to Fc receptors and stained with fluorochrome-conjugated monoclonal antibodies (mAbs ...
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bioRxiv - Immunology 2020Quote: ... Blocking was performed by adding 20 μg/ml anti-CD16/32 (Biolegend #101301) and incubated for 15 minutes on ice in dark ...
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bioRxiv - Immunology 2021Quote: ... and seeded into 2 μg/mL anti-CD3 antibody (clone: 145-2C11, BioLegend) pre-coated 24-well plates ...
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bioRxiv - Cancer Biology 2022Quote: ... “Rescue” wells were treated with 0.8 ug/mL recombinant human periostin (BioLegend 770506). Scratches were imaged at 0 hours ...
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bioRxiv - Cancer Biology 2022Quote: ... we added 4 μg/ml PE-labeled anti-CD63 antibody (BioLegend, clone H5C6). We imaged the nanowell using Zeiss Axio Observer Z1 microscope equipped with 20x/0.8 NA objectives and a Hamamatsu Orca Flash v2 camera.
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bioRxiv - Cancer Biology 2022Quote: ... 2.5 mM CaCl2] containing 0.8 μg/mL APC-labeled annexin A5 (640919, BioLegend) or 4.8 μg/mL FITC-labeled GlaS and mixed for 45 min at room temperature ...
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bioRxiv - Microbiology 2022Quote: ... The cells were then stained with 0.5 µg/ml annexin V-PE (BioLegend) and 1 µg/ml LIVE/DEAD™ fixable near-IR dye in annexin V binding buffer for 15 minutes ...
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bioRxiv - Microbiology 2022Quote: ... IFN-gamma (IFN-γ) PE/Dazzle 594 (0.6 μg/mL, BioLegend Cat. 505845), and TNF-alpha (TNF-α ...
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bioRxiv - Immunology 2022Quote: ... alone or in combination with 2ug/mL biotinylated anti-CD28 (Clone 37,15, BioLegend) for 15min at room temperature ...
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bioRxiv - Immunology 2022Quote: ... and incubated with 1 μg/mL biotinylated anti-CD28 antibody (Clone 37.15, BioLegend) PBS+2%FCS for 30 minutes on ice to allow binding ...
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bioRxiv - Cell Biology 2024Quote: ... Red blood cells were removed by adding 1 mL RBC lysis buffer (Biolegend) to each tube and incubate at room temperature for 1 minute.
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bioRxiv - Bioengineering 2024Quote: ... and then activated by pre-coated plate-bound anti-CD3 (1μg/ml; Biolegend) and soluble anti-CD28 ((1μg/ml ...