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Citations for BioLegend :
2951 - 3000 of 3545 citations for IL 17A Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Microbiology 2021Quote: ... CD4+ T cells were isolated by negative selection using MojoSort Human CD4 T Cell Isolation kit (Biolegend). Primary CD4+ T cells were maintained in complete RPMI 1640 medium supplemented with 10 IU/ml IL-2 (Center for AIDS Reagents [CFAR] ...
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bioRxiv - Microbiology 2020Quote: ... cells were stained for 30 min at 4°C with FITC-conjugated anti-human CD3 antibody (Biolegend), followed by fixation and permeabilization of cells with BD Cytofix/Cytoperm™ (BD Bioscience) ...
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bioRxiv - Immunology 2021Quote: ... CD4+ cells were isolated by negative-selection using MOJOSORT™ Human CD4 T Cell Isolation Kit (Biolegend) and a EASYSEP™ Magnet (StemCell ...
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bioRxiv - Immunology 2021Quote: ... PE anti-human HLA-A,B,C (clone W6/32; dilution 1:30; Biolegend; Cat no 311406), PerCP/Cyanine5.5 anti-human CD3 (clone SK7 ...
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bioRxiv - Cancer Biology 2022Quote: ... T cells were separated from PBMCs using a MojoSort human CD3+ T cell isolation kit (Biolegend, USA).
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bioRxiv - Immunology 2022Quote: ... and Alexa Fluor 647-anti mouse/human CD324 (E-cadherin) (clone DECAMA-1) was obtained from Biolegend.
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bioRxiv - Immunology 2022Quote: ... Human transfected HLA-A2.1+ CD8+ T cells were stained with: anti-mouse TCR-β-FITC (Biolegend, 109205), anti-mouse TCR-β-BV421 (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... in the presence or absence of a mouse anti-human PD1 (2 μg/mL) blocking antibody (Biolegend). CD4+ T-cells were identified using a mouse-anti human CD4 (OKT4 clone ...
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bioRxiv - Immunology 2022Quote: ... and MDA-231 cells were stained with APC conjugated anti-human CD340 (erbB2/HER-2) Antibody (Biolegend). MR1 expression on T2 cells was assessed with anti-MR1-PE (Biolegend) ...
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bioRxiv - Cancer Biology 2024Quote: ... Alexa-fluor 488 anti-mouse CD49b (Clone HMα2; 103510) and mouse anti-human LAMP2 (354303) from BioLegend, primary antibodies for mouse anti-human CD49b (611017 ...
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bioRxiv - Neuroscience 2022Quote: ... Recovered cells were stained anti-mouse/human CD11b antibody clone M1/70 (BV605; Biolegend, San Diego, CA), washed ...
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bioRxiv - Cell Biology 2022Quote: ... cells were incubated with Alexa Fluor 488 anti-mouse/human Mac-2 (Galectin-3) antibody (125410, Biolegend) or alternatively with Brilliant Violet 421 anti-mouse/human Galectin-3 antibody (125416 ...
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bioRxiv - Cancer Biology 2024Quote: ... T cells were isolated using the MojoSort Human CD3 T Cell Isolation Kit (cat. no. 480021, Biolegend) and EasySep Magnet (cat ...
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bioRxiv - Cell Biology 2023Quote: ... U266B1 and IM-9 cells were evaluated directly with PE-conjugated anti-human DR4 antibody (B376455, Biolegend) and PE-conjugated anti-human DR5 antibody (B347680 ...
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bioRxiv - Immunology 2024Quote: ... Donor 5 and 6 PBMCs were stained with a cocktail of anti-human CD3 PerCP-Cy5.5 (Biolegend), CD8 PerCP-Cy5.5 (Biolegend) ...
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bioRxiv - Immunology 2024Quote: ... in complete medium supplemented with anti-human CD28 antibody (5 µg/mL) (BioLegend, San Diego, CA, US), rhIL2 (50 U/mL ...
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bioRxiv - Immunology 2024Quote: ... T cell activation was assessed by incubating cells with fluorescein isothiocyanate-conjugated anti-human CD8 (Biolegend, 100705) and phycoerythrin-conjugated anti-human CD25 (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... 1 mL 50 mg/mL PE/Dazzle594-mouse-anti-human CD16 (BioLegend; Cat. No. 302054; clone 3G8), 1 mL 100 mg/mL PE/Cy7-mouse-anti-human CD39 (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... 0.5 mL 200 mg/mL APC/Cy7-mouse-anti-human CD3 (BioLegend; Cat. No. 317342; clone OKT3), 2 mL 200 mg/mL BV711-mouse-anti-human CD49a (BD Biosciences ...
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bioRxiv - Immunology 2024Quote: ... 1 mL 100 mg/mL PE/Cy7-mouse-anti-human CD39 (BioLegend; Cat. No. 328212; clone A1), 1 mL 50 mg/mL APC-mouse-anti-human CD56 (BioLegend ...
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bioRxiv - Immunology 2024Quote: ... anti-human CD45 (clones T29/33 and BC8 from Leinco Technologies; clones 2D1 and HI30 from BioLegend).
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bioRxiv - Immunology 2023Quote: ... stable transfectants were screened by flow cytometry using mouse anti human PD1 (cat: 621610, BioLegend, CA, USA) and isotype control using allophycocyanin (APC)-conjugated mouse IgG1(cat ...
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bioRxiv - Immunology 2023Quote: Engineered T-cells were stained with increasing concentrations of AF647-conjugated anti-human αβTCR antibody (IP26: Biolegend) and HLA.A2/9V-AF647 for determination of label saturation concentration of CMV-specific RA14 TCR T-cells and HLA.A2/NY-ESO-1-specific engineered T-cells ...
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bioRxiv - Immunology 2023Quote: ... Target cell lines were stained using phycoerythrin (PE)-conjugated mouse anti-human PDL1 antibody (cat: 329706, BioLegend) and APC-conjugated mouse anti-human PDL2 antibody (cat ...
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bioRxiv - Cancer Biology 2023Quote: ... The pellet was resuspended in red blood cell lysis buffer (BioLegend, catalog no. 420301, all human samples) and incubated for 3 min on ice with periodic shaking ...
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bioRxiv - Immunology 2023Quote: ... supernatants from cell cultures were harvested for detecting cytokine using LEGENDplexTM Human Th1 Panel (5-Plex) (BioLegend). Samples were diluted 5-fold using assay buffer and then mixed with beads and shaken for 2 hours at 500 rpm in a 96 plate well ...
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bioRxiv - Genetics 2023Quote: ... splenic B cells were collected and stained with FITC anti-mouse/human GL7 (Biolegend, Cat. No. 144604) and PE hamster anti-mouse CD95 (BD ...
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bioRxiv - Cancer Biology 2023Quote: ... Fc-block was performed by incubating cells in a dilution of Human TruStain FcX (#422302, BioLegend, USA) and mouse TruStain FcX (#101320 ...
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bioRxiv - Microbiology 2023Quote: ... for 30 minutes at RT after adding Human TruStain FcX (1:200 dilution, Biolegend, San Diego, CA) for 5 minutes ...
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bioRxiv - Biophysics 2023Quote: ... Antibodies used for cell labeling were as follows: APC anti-human CD18 (clone LFA-1/2, Biolegend), and Propidium Iodide solution (Biolegend) ...
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bioRxiv - Immunology 2023Quote: ... we used Ultra-LEAF™ purified anti-human TIM-3 antibody (10μg/ml, clone F38-2E2, Biolegend) or its respective isotype Ultra-LEAF™ purified mouse IgG1 antibody (10μg/ml ...
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bioRxiv - Cancer Biology 2023Quote: ... and Brilliant Violet 510™ anti-human CD45 (clone HI30, dilution 1/20, Biolegend, Amsterdam, The Netherlands) antibodies were used for progenitor cell subset phenotyping ...
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bioRxiv - Immunology 2023Quote: ... Commercial anti-RBD antibodies (mouse IgG1κ, clone 1035753, R&D systems or human IgG1κ, clone AM001414, BioLegend) and isotype controls (mouse IgG1κ ...
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bioRxiv - Neuroscience 2023Quote: ... Concentrations of chemokines in cell supernatants were measured using the LEGENDplexTM Human Proinflammatory Chemokine Panel 1 (Biolegend). Readings were made on an AttuneTM Nxt Flow Cytometer.
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bioRxiv - Immunology 2023Quote: ... Intracellular proteins were stained using the following antibodies: anti-human T-bet PE (1:20, 4B10, BioLegend), and anti-human Ki-67 BUV395 (1:50 ...
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bioRxiv - Genomics 2023Quote: ... The PBMC pellet was resuspended and cells were stained with Human TruStain FcX Fc Blocking Reagent (BioLegend) for 10 min at 4°C and then with fixable viability dye DAPI (Merck) ...
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bioRxiv - Microbiology 2023Quote: ... Cells were incubated with 2 μg/mL of phycoerythrin (PE) anti-human IgG Fc (Clone M1310G05, BioLegend). Cells were washed once ...
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bioRxiv - Cancer Biology 2023Quote: TCR-transduced cell lines were stained with anti-human TCR α/β (Clone IP26, BioLegend Cat. #306717) and anti-human CD3 antibody (Clone HIT3a ...
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bioRxiv - Genetics 2024Quote: ... MDA-MB-231 and B80-T5 by flow cytometry (Biolegend, PE anti-human CD340 (erbB2/HER-2) Antibody ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were randomized on the assay plates and analyzed using the LEGENDplex human CD8/NK panel (BioLegend). Samples were read on a CytoFLEX S flow cytometer (Beckman Coulter) ...
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bioRxiv - Neuroscience 2024Quote: ... the iAstrocytes were stained with 1:50 PE anti-human HLA-A,B,C Antibody (BioLegend, 311406) and DAPI for 30 minutes at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... or Anti-human/mouse CD49f antibody (#313620, Biolegend) (isotype control, Pacific Blue Rat IgG2a, k; #400527, Biolegend). For GD3 FACS analysis ...
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bioRxiv - Cancer Biology 2024Quote: ... a single-cell solution of lung cells was incubated with Human TruStain FcX (Biolegend, #422302, 1:20) for 10 minutes ...
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bioRxiv - Genomics 2024Quote: ... for 10 minutes at 4 °C and then stained with FITC - anti-human EPCAM (BioLegend CAT #32404) and PE - anti-CD45 (BioLegend ...
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bioRxiv - Cancer Biology 2021Quote: ... The following antibodies were used: Alexa Fluor® 488 anti-human CD45 antibody (clone H130, catalog #304019, BioLegend); APC anti-human CD3 antibody (clone HIT3a ...
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bioRxiv - Immunology 2021Quote: Freshly thawed decidual immune cells (1-2 10e6 cells) were incubated with Fc blocker (Human TruStain FcX, Biolegend) in PBS with 1% BSA for 10 minutes at 4C and then surface stained with CD45-FITC (HI30 ...
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bioRxiv - Developmental Biology 2021Quote: ... The following antibodies and dilutions were used: anti-human CD172a/b (SIRPα/β) (Biolegend; clone SE5A5; 1:200) anti-human CD13 (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... B cells were washed with FACS buffer and stained with PerCP-cy5.5 anti-human CD19 antibody (Biolegend, 363016), FITC anti-human CD3 antibody (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... for 1 hour and subsequently stained overnight at 4°C with mouse anti-human TCR Vα7.2-PE (Clone 3C10, Biolegend), mouse anti-human CD161-AF488 (Clone NKR-P1A ...
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bioRxiv - Bioengineering 2019Quote: ... Samples were thawed on ice prior to detection and analysis using LEGENDplex human hematopoietic stem cell panel (BioLegend), according to manufacturer’s protocol.