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Citations for BioLegend :
2851 - 2900 of 3545 citations for IL 17A Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2020Quote: Human PBMCs were isolated by Ficoll and incubated with antibodies against CD3 (OKT3, BioLegend unless indicated), CD4 (RPA-T4) ...
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bioRxiv - Immunology 2019Quote: ... suspended in PBS containing 1% BSA and an Fc receptor-blocking (human TruStainFcX; Biolegend, CA, USA). The neutrophils were then permeabilized with 0.1% saponin and incubated with a mAb against TLR9 conjugated with FITC (catalog number ab134369/clone 26C593.2 ...
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Blunted Fas signaling favors RIPK1-driven neutrophil necroptosis in critically ill COVID-19 patientsbioRxiv - Immunology 2021Quote: ... Ultra-LEAF™ purified anti-human CD95 (FAS) blocking antibody (10 μg/ml, clone A16086F, Biolegend) or respective DMSO and H2O control were added prior to incubation ...
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bioRxiv - Immunology 2019Quote: ... at 0.1μg/ml each in 100μl total volume: AF647-conjugated anti-human/mouse CD11b (#101220, BioLegend), PE-conjugated anti-mouse Ly6C (#101220 ...
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bioRxiv - Microbiology 2019Quote: ... were subjected to CD14+ magnetic bead positive selection using the MojoSort Human CD14 Selection Kit (BioLegend). Purified CD14+ monocytes were cultured in complete RPMI supplemented with 100ng/mL each of recombinant human IL-4 and GM-CSF (PeproTech ...
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bioRxiv - Developmental Biology 2020Quote: ... Guinea pig anti-Cytokeratin 8+18 antibody and FITC rat anti-mouse/human CD45R/B220 (BioLegend) were used for fluorescence immuno-histochemistry ...
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bioRxiv - Genomics 2021Quote: ... 4 µl of the respective antibodies were used: APC anti-human SSEA-4 antibody (Biolegend, #330417) and PE anti-human TRA-1-60-R antibody (Biolegend ...
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bioRxiv - Microbiology 2020Quote: ... cells were stained with Alexa Fluor® 647-conjugated anti-human IFN-γ antibody (502516, Biolegend) and PE-conjugated anti-human IL-17A antibody (512305 ...
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bioRxiv - Immunology 2021Quote: ... Cytokine levels were analyzed by LegendPlex TGF-β 1 and multiplex human inflammation panel assay (Biolegend). All nine parameters were detectable in every sputum sample derived from patients with AR and AA and compared to healthy control subjects.
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bioRxiv - Immunology 2020Quote: ... BMDM were labeled with APC anti-mouse/human CD11b (1:400, Cat. 101211, BioLegend CA, USA) and PE anti-mouse CD45 (1:400 ...
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bioRxiv - Immunology 2021Quote: ... eBioscience Cat#12-1239-42) and CD304 (BV421 anti-human CD304, clone 12C2, BioLegend Cat#354514) (Appendix Figure S1) ...
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bioRxiv - Immunology 2022Quote: ... Jurkat cells were labelled with 25 μg/ml anti-human CD3 antibody (BioLegend, 317304, clone OKT3) and 3 μg/ml recombinant annexin V (BioLegend ...
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bioRxiv - Biochemistry 2022Quote: ... Cells were incubated with 1 μg/mL of phycoerythrin (PE)-conjugated anti-human IgG Fc (BioLegend). Cells were washed once and resuspended in ice-cold FACS buffer ...
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bioRxiv - Cancer Biology 2022Quote: ... MHC-II (LGII-612.14, CST, Catalog: 68258S) and Anti-human pan-Cytokeratin-Alexa647 (Biolegend, Catalog: 628604) were used for the staining ...
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bioRxiv - Genomics 2022Quote: ... resuspended in chilled 1% BSA in PBS and incubated with human TruStain FcX blocking solution (BioLegend) for 10 minutes at 4°C ...
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bioRxiv - Immunology 2022Quote: ... Cytokine secretion in the supernatant was evaluated by ELISA MAX Deluxe Set Human TNF-α (BioLegend), ELISA MAX Deluxe Set Human IFN-γ (BioLegend) ...
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bioRxiv - Immunology 2022Quote: ... Monocytes were cultured in 10 ng/mL recombinant human GM-CSF or M-CSF (both Biolegend) for 7 days at 37°C ...
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bioRxiv - Immunology 2022Quote: ... Monocytes were cultured in 10 ng/mL recombinant human GM-CSF or M-CSF (both Biolegend) for 7 days at 37°C ...
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bioRxiv - Genetics 2022Quote: ... for SynT and HLAG (1:100, APC anti-human HLA-G Antibody Clone: 87G, BioLegend UK) for EVT ...
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bioRxiv - Microbiology 2022Quote: ... blocked with Fc Block for 20 min at room temperature (Human TruStain FcX, Biolegend Cat# 422302), and simultaneously stained for surface antigenic Site Ø ...
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bioRxiv - Immunology 2022Quote: ... and stained again with biotinylated anti-mouse/human TLR7/8 mAb and PE streptavidin (Biolegend, USA). To detect intracellular mouse IL-6 in splenocytes stimulated with various ligands in the presence of Brefeldin A (10μg/ml) ...
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bioRxiv - Cell Biology 2024Quote: ... or 2.5 µl of fluorescein isothiocyanate (FITC)-conjugated anti-human CD41/CD61 (clone PAC-1, BioLegend) for 2 h at room temperature (RT ...
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bioRxiv - Bioengineering 2024Quote: Quantification of secreted cytokines was conducted using the 13-plex LEGENDplex Human Macrophage/Microglia panel (BioLegend) designed for the identification of IL-12p70 ...
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bioRxiv - Genomics 2024Quote: ... and stained with the TotalSeq-A Human Universal Cocktail panel according to the manufacturer’s instructions (BioLegend).
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bioRxiv - Microbiology 2023Quote: ... 2 million PBMC of each sample were first blocked with Human TruStain FcX (BioLegend; catalog #422301), then incubated with 40ng of various anti-human hashtag antibodies carrying unique cell barcodes and 12-100ng of various CITE-seq antibodies ...
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bioRxiv - Genomics 2024Quote: ... then resuspended in 105μL of cell staining buffer with 1:21 Human TruStain FcX (BioLegend 422301), followed by incubation at 4°C for 20 mins at 300rpm ...
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bioRxiv - Immunology 2024Quote: ... 1 mL 50 mg/mL APC-mouse-anti-human CD56 (BioLegend; Cat. No. 318310; clone HCD56), 0.5 mL 200 mg/mL APC/Cy7-mouse-anti-human CD3 (BioLegend ...
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bioRxiv - Developmental Biology 2023Quote: ... and incubated with the monoclonal anti-human antibodies anti-CD56-PE and anti-CD82-Alexa647 (BioLegend). After 30 minutes incubation on ice ...
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bioRxiv - Biophysics 2024Quote: ... PBMCs for microscopy measurements of monocytes were incubated with Alexa Fluor 647 anti-human CD14 (Biolegend) following the protocol described above and stained with Pro12A for GP quantification.
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bioRxiv - Immunology 2023Quote: ... These slides were incubated overnight with anti-human CD34 FITC (Clone 581, Biolegend 343504, 1:200), anti-human/mouse ACTA2 Cy3 (Clone 1A4 ...
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bioRxiv - Systems Biology 2022Quote: ... and resuspended in 25μL cell staining buffer and 25ul of TotalSeqA Human Universal Cocktail v1.0 (BioLegend) for 30min on ice ...
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bioRxiv - Neuroscience 2023Quote: ... HPCs were validated by staining them with 1:100 dilution of CD34 (anti-human FITC, Biolegend), CD43 (anti-human PE ...
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bioRxiv - Neuroscience 2022Quote: ... 4B12 [a mouse monoclonal antibody that recognises residues 103-108 of human α-synuclein (12)] (BioLegend) at 1:250 and MJFR14 [a rabbit monoclonal antibody that has been reported to recognise assembled human α- synuclein (15)] (Abcam ...
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bioRxiv - Immunology 2023Quote: ... the supernatant from cocultures was collected and measured by LEGENDplex™ Human CD8/NK Panel (Biolegend) according to the manufacturer’s instructions.
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bioRxiv - Microbiology 2023Quote: The antibodies used for flow cytometry are Alexa Fluor 647 mouse anti-human BST2 (Biolegend; 348404) and HIV-1 core antigen-FITC (KC57 ...
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bioRxiv - Immunology 2023Quote: ... Adoptively transferred human PBMC were stained with the following antibodies: CD4-PerCP-Cy5.5 (BioLegend, clone SK3), CD8a-APC-Cy7 (BioLegend ...
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bioRxiv - Microbiology 2023Quote: ... the infected cells were stained with a biotinylated anti-human CD271 (NGFR) antibody (BioLegend, Cat# 345122). Subsequently ...
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bioRxiv - Immunology 2023Quote: ... BV421™ anti-mouse/human CD45R/B220 Antibody (Clone RA3-6B2; BioLegend; Cat#103251; RRID: AB_2562905) BV605 Mouse Anti-Mouse CD45.1 (Clone A20 ...
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bioRxiv - Microbiology 2023Quote: ... infected or stimulated cells using a LEGENDplex Human Type 1/2/3 Interferon Panel assay (BioLegend) according to the manufacturer’s protocol.
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bioRxiv - Bioengineering 2023Quote: ... Samples were then stained for 30 minutes with: Human anti-CD3 (1:400; BioLegend, clone: SK7), human anti-CD4 (1:400 ...
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bioRxiv - Immunology 2023Quote: ... and the following antibodies: rabbit monoclonal anti-mouse/human Mac-2 (clone M3/M8; Biolegend #125401), rabbit monoclonal anti-mouse CD4 (clone EPR19514 ...
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bioRxiv - Cell Biology 2023Quote: ... and CF568-labeled antibodies specific for human CD9 (BioLegend, Cat. No. 312102, San Diego, CA, USA) and human CD81 (BioLegend ...
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bioRxiv - Immunology 2023Quote: ... Cells were washed and stained with 1:100 diluted anti-human CD66b-Pacific Blue (BioLegend, 305112). Cells were washed and bead uptake was measured on a BD LSR II (BD Biosciences) ...
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bioRxiv - Cancer Biology 2023Quote: ... Brilliant Violet 510™ anti-human CD45 (clone HI30, dilution 1/20, Biolegend, Amsterdam, The Netherlands), APC anti-human CD206 (clone 19.2 ...
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bioRxiv - Cancer Biology 2023Quote: ... Brilliant Violet 510™ anti-human CD45 (clone HI30, dilution 1/20, Biolegend, Amsterdam, the Netherlands), Brilliant Violet 450 anti-human CD3 (Clone UCHT1 ...
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bioRxiv - Cancer Biology 2023Quote: ... Samples were used directly for cytokine analysis with the LEGENDPlex Human Inflammatory Panel 1 (BioLegend, 740809) according to the manufacturer’s protocol ...
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bioRxiv - Cancer Biology 2023Quote: ... T cells were isolated and stained with fluorochrome-labeled anti human CD3 (BioLegend, Cat. No. 317342) and anti human CD8a (BioLegend ...
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bioRxiv - Cancer Biology 2023Quote: ... single cell suspensions were labelled with TotalSeqA anti-human hashtag oligonucleotide (HTO) antibodies (Biolegend, 1:50) to allow downstream de-multiplexing in silico as previously described9 ...
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bioRxiv - Immunology 2023Quote: ... Cultures were treated with anti-human CD2 mAb (10µg/mL; Cat# 300240; Clone: RPA-2.10; Biolegend) or IgG (10 µg/mL ...
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bioRxiv - Bioengineering 2023Quote: ... All flow cytometry anti–human mouse Ab probes and ZombieGreenTM were purchased from Biolegend (CA, USA). UltraComp eBeadsTM Plus Compensation beads (ThermoFisher Scientific ...