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Citations for BioLegend :
2701 - 2750 of 2969 citations for Rat Orexin Receptor Type 2 HCRTR2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cancer Biology 2021Quote: ... MHCll-APC/Cy7 (clone M5/114.15.2) and Live/Dead Zombie UVTM Fixable viability kit were purchased from Biolegend. All antibodies were tested with their isotype controls ...
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bioRxiv - Immunology 2021Quote: ... Staining for FOXP3 followed the instruction provided by True-Nuclear Transcription Factor Buffer Set kit (BioLegend, Cat#: 424401). For apoptosis analysis ...
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bioRxiv - Cell Biology 2020Quote: ... naïve CD4+ T cells isolated from OTII/CD45.2 TCR transgenic donor mice using naïve CD4+ mouse T cell isolation kit (Biolegend) were labeled with CFSE ...
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bioRxiv - Immunology 2021Quote: Splenic B cells were isolated from bone marrow chimeras (MojoSort™ Mouse Pan B Cell Isolation Kit; Biolegend). Control and WNK1-deficient B cells were labelled with CellTracker blue (CMAC ...
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bioRxiv - Developmental Biology 2020Quote: ... and labeled using a FITC Annexin V and propidium iodide staining kit as per manufacturer’s protocol (BioLegend, 640914). Both EdU- and Annexin V-labeled samples were analyzed on a LSRII flow cytometer (BD Biosciences ...
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bioRxiv - Developmental Biology 2021Quote: The Annexin V staining was performed using the FITC Annexin V apoptosis detection kit with PI (BioLegend, USA). The cells were washed with PBS followed by washing with staining buffer and resuspension in binding buffer containing anti-Annexin V and PI and incubation for fifteen minutes at room temperature in the dark ...
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bioRxiv - Immunology 2021Quote: ... the cells were washed with 1xPBS and fixed using buffer in True-Nuclear Transcription Factor Staining kit (Biolegend). After fixing ...
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bioRxiv - Immunology 2020Quote: ... Fluorescence labeled antibodies (CD209,CD206 et al) and multi cytokines kit were from Biolegend (San Diego, CA, USA). Specific inhibitors were acquired from MedChemExpress (Monmouth Junction ...
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bioRxiv - Cancer Biology 2020Quote: ... fixed and permeabilized using BD Cytofix/Cytoperm kit and then stained for IL-10 expression (JES3-19F1, Biolegend). Flow cytometry was performed on LSR Fortessa (BD Biosciences ...
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bioRxiv - Immunology 2022Quote: ... naïve CD4+ T cells isolated from OTII/CD45.2 TCR transgenic donor mice using naïve CD4+ mouse T cell isolation kit (Biolegend) were labeled with CFSE ...
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bioRxiv - Microbiology 2022Quote: IL-1 cytokine concentrations in supernatants from cultured BMDMΦ were assessed using the Legendplex mouse inflammation kit (BioLegend) following the manufacturer’s instructions as described previously (31,50).
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bioRxiv - Cancer Biology 2019Quote: Apoptosis detection was carried out using the FITC Annexin V Apoptosis Detection Kit with PI (640914, Biolegend, USA). Cells were washed twice with cold BioLegend’s Cell Staining Buffer (Biolegend) ...
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bioRxiv - Cancer Biology 2019Quote: One million cells were stained in 100µL of Zombie Aqua (Fixable Viability Kit BioLegend; San Diego, California, USA) for twenty minutes at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... After a quick wash with DPBS cells were stained with Zombie Red™ Fixable Viability Kit (#423110, BioLegend) according to manufacturer’s instructions ...
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bioRxiv - Developmental Biology 2021Quote: Apoptotic analysis was determined using the FITC Annexin V apoptosis detection kit with PI (Propidium Iodide) (Biolegend 640914) and performed according to the manufacturer’s manual ...
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bioRxiv - Cancer Biology 2021Quote: ... cells were stained with the appropriate surface markers [dead cell marker-Zombie Aqua™ Fixable Viability Kit (Biolegend)] and CD8 (APC conjugated ...
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bioRxiv - Immunology 2021Quote: ... Lung cytokine levels were assessed from the supernatant samples via LEGENDplex kit (mouse inflammation panel 13-plex; BioLegend) per manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: Naïve CD4+ T cells were isolated from spleens and lymph nodes of BDC-2.5 transgenic NOD mice using the Mouse CD4 Naïve T Cell Isolation Kit (BioLegend). Naïve T cells were further selected positively by treatment with anti-CD62L biotinylated antibodies (BD Pharmingen ...
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bioRxiv - Immunology 2020Quote: ... Sera were assayed for cytokines using a 13-plex LEGENDplex Mouse Inflammation Panel kit (Biolegend, San Diego, CA) following the manufacturer’s instructions using an Accuri C6 flow cytometer (BD Bioscience ...
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bioRxiv - Immunology 2020Quote: ... Cell death was analysed 18h later by FITC Annexin V/PI Apoptosis Detection Kit with PI (640914; Biolegend).
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bioRxiv - Immunology 2021Quote: ... CD3+ cell enrichment was achieved by MojoSort negative selection using MojoSort mouse CD3+ T-cell Isolation Kit (BioLegend) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... the isolated B cells were incubated with 1 μl Zombie UV Fixable Viability kit (Biolegend, cat. no. 423107) per 1 ml cell suspension for 30 min on ice ...
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bioRxiv - Immunology 2022Quote: CD4+ T cells (naïve or total) were enriched with naïve or total CD4+ T cell negative enrichment kits (Stemcell, Biolegend) from the spleens of female ...
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bioRxiv - Systems Biology 2023Quote: ... Dead cells were stained with Fixable Viability Dye eFluor 780 (eBioscience) or Zombie Violet Fixable Viability Kit (BioLegend). Cells were stained with the following fluorochrome-conjugated anti-human Abs ...
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bioRxiv - Immunology 2022Quote: ... Naïve CD4 T cells were presorted with the MojoSort™ Mouse CD4 Naïve T cell Isolation kit (BioLegend) based on provided directions ...
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bioRxiv - Immunology 2022Quote: ... Twelve days post-treatment tumors were dissociated and subjected to CD45.1 positive selection (MojoSort mouse CD45.1 selection kit, Biolegend) per the manufacterer’s instructions ...
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bioRxiv - Neuroscience 2024Quote: Cell suspensions were first stained for viability using Zombie NIR™ Fixable Viability Kit (BioLegend; San Diego, CA) for 20 min at room temperature in phosphate buffer solution (pH 7.2S ...
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bioRxiv - Immunology 2024Quote: ... and IL-6 were measured by LEGENDplex flow-based 13-plex mouse inflammation panel kit from Biolegend (740446) according to manufacturer’s instructions ...
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bioRxiv - Immunology 2024Quote: ... antibody or 100µL of PBS containing 0.2µg TruStain FcX™ and 0.04µL Zombie AquaTM Fixable Viability Kit (BioLegend cat#: 423102) and incubated at room temperature (RT ...
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bioRxiv - Immunology 2024Quote: ... mast cells were stained using CD117 (c-Kit)-APC or APC/Cy7 and FcεRIα-PE (all from Biolegend) at 1:200 dilution for 20 min on ice ...
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bioRxiv - Immunology 2022Quote: ... naïve CD4 T cells were magnetically isolated from the spleen and LNs of Rag2-/- TCR7B8 Tg or TCR1A2 Tg mice on CD45.2/CD45.2 background using the MojoSort™ Mouse CD4 Naïve T cell Isolation kit (BioLegend). Then ...
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bioRxiv - Cell Biology 2023Quote: ... and T cells were isolated from the spleen using the MojoSortTM Mouse CD3 T cell Isolation Kit (Biolegend). Experiments where purified T cells were cultured for longer than 24 hours ...
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bioRxiv - Microbiology 2023Quote: ... Blood or tonsil CD4+ T cells were isolated using a human CD4+ T cell isolation kit (BioLegend #480010). Purified CD4+ T cells were used without stimulation or co-stimulated with plate-bound CD3 and soluble CD28 antibodies in the presence of 20 ng/ml IL-2 for three days ...
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Fine tuning of CpG spatial distribution with DNA origami for improved therapeutic cancer vaccinationbioRxiv - Synthetic Biology 2023Quote: ... CD8 OT-I cells and CD4 OT-II were isolated by MojoSort kit purchased from BioLegend (#480035, #480033) according to the manufacturer protocol ...
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bioRxiv - Immunology 2023Quote: ... CD8+ T cells were isolated by negative selection using the MojoSort Mouse CD8+ T cell isolation kit (BioLegend) and cultivated as described above ...
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bioRxiv - Molecular Biology 2023Quote: ... cells were subjected to a live/dead staining with the Zombie NIR™ Fixable Viability Kit (423105; BioLegend) and incubated in the dark for 10 min ...
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bioRxiv - Cell Biology 2023Quote: ... 0.5% BSA and 0.07% NaN3 in PBS) with additional Zombie Aqua™ (1:1,000 Fixable Viability Kit, BioLegend) to stain for vital cells ...
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bioRxiv - Genomics 2023Quote: ... CD4+ T-cells were then isolated using the human CD4+ T-cell MojoSort negative magnetic selection kit (Biolegend). Enriched CD4+ T-cells were then rested overnight in a 37°C 5% CO2 incubator in complete RPMI (RPMI1640 ...
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bioRxiv - Immunology 2023Quote: ... both human and murine single cell suspensions were first stained with a viability marker (Fixable Viability Kit, Biolegend) according to manufacturer’s instructions to exclude dead cells ...
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bioRxiv - Neuroscience 2023Quote: ... Cell suspensions were first stained for viability using Zombie NIR™ Fixable Viability Kit (BioLegend, San Diego, CA) for 20 min at room temperature in PBS combined with FcR blocking antibody (1 μg ...
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bioRxiv - Molecular Biology 2023Quote: Cells were trypsinized and stained with Annexin V and 7-AAD using APC Annexin V staining kit (BioLegend) according to the manufacturer’s instructions ...
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bioRxiv - Microbiology 2023Quote: Naïve B cells were isolated from spleens by immunomagnetic depletion using MojoSort Pan B cell Isolation Kit (Biolegend) and cultured in RPMI supplemented with L-glutamine ...
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bioRxiv - Microbiology 2023Quote: ... resuspended in 100 μL Zombie NIR (1:1000 in PBS, Zombie NIR™ Fixable Viability Kit, Biolegend, #423106), transferred into a 96-well plate and incubated for 20 min at 4 °C in the dark ...
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bioRxiv - Cell Biology 2024Quote: ... The samples were subjected to staining using the Annexin V/Propidium Iodide (PI) apoptosis assay kit (BioLegend, USA) and subsequently analyzed using a BD FACSCalibur flow cytometer (USA) ...
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bioRxiv - Physiology 2024Quote: ... using the Pacific Blue Annexin V Apoptosis Detection Kit with 7-amino-actinomycin D (7-AAD) (BioLegend, #640926), according to the manufacturer’s instructions.
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bioRxiv - Bioengineering 2024Quote: ... cells were washed once more with PBS and stained with the Zombie VioletTM Fixable Viability Kit (Biolegend, USA) for 15 min following manufacturer’s instructions ...
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bioRxiv - Bioengineering 2024Quote: ... and cell pellets were washed with PBS before being treated with the Zombie NIRTM Fixable Viability Kit (Biolegend) following the manufacturer’s instructions ...
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Downregulation of Let-7 miRNA promotes Tc17 differentiation and emphysema via de-repression of RORγtbioRxiv - Immunology 2024Quote: CD8+ naïve T cells were isolated from spleen using Mojosort Mouse CD8 Naïve T cell isolation Kit (Biolegend) and adjusted to a concentration of 1.0×106 cells/mL ...
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bioRxiv - Immunology 2024Quote: ... cells were washed with PBS and stained for dead cells using Zombie UV Fixable Viability Kit (Biolegend, 423108) or Zombie NIR Fixable Viability Kit (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... Single cell suspensions were stained with the Zombie NIR™ Fixable Viability KIT (780nm of excitation; Biolegend, #423106) at a 1/250 dilution in PBS (Biolegend ...