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Citations for BioLegend :
2451 - 2500 of 2969 citations for Rat Orexin Receptor Type 2 HCRTR2 ELISA Kit since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Neuroscience 2020Quote: ... 50μM coronal sections containing dorsal hippocampus were blocked in normal goat serum for 2 h and incubated overnight using a biotin conjugated anti-HA (Biolegend 901505, 1:500), anti-pS6 244-247 (ThermoFisher 44-923G ...
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bioRxiv - Cancer Biology 2020Quote: ... coated with intercellular adhesion molecule (ICAM-1, ICAM-2) and vascular cell adhesion molecule (VCAM-1) antibodies conjugated to Alexa Fluor 680 (BioLegend, San Diego, CA). Sorted cells were cultured in Endothelial Growth Media (EGM-2 ...
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bioRxiv - Immunology 2022Quote: ... extracellular staining was done in ice-cold buffer (phenol red–free DMEM, 2% FBS) for 30 min using antibodies against CD45 (30-F11; BioLegend, dilution 1:200), CD4 (RM4-5 ...
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bioRxiv - Immunology 2022Quote: ... 2×107 cells resuspended in 0.5 ml PBS were incubated with PE-conjugated mouse anti-human CD15 antibody (BioLegend, San Diego, CA, USA) diluted 1:40 ...
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bioRxiv - Immunology 2021Quote: ... cells were washed with staining buffer (PBS containing 2% FBS) and surface stained with anti-mouse-CD4-PerCP/Cy5.5 (Biolegend Cat# 116012, RRID: AB_2563023), anti-mouse-CD8-BV785 (Biolegend Cat# 100750 ...
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bioRxiv - Microbiology 2021Quote: ... approximately 200,000 cells per sample were resuspended in staining buffer (PBS, 2% BSA, 0.01% Tween) and incubated for 10 minutes with Fc block (TruStain FcX, Biolegend; FcR blocking reagent, Miltenyi). Cells were then incubated with barcoded hashing antibodies for 30 min at 4 °C ...
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bioRxiv - Immunology 2022Quote: ... Sorted naïve 2D2 CD4+ T cells were activated with plate-coated 2 μg/mL anti-mouse CD3e mAb (clone 145-2C11, BioLegend, San Diego, CA) and soluble 1 μg/mL anti-mouse CD28 mAb (clone 37.51 ...
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bioRxiv - Pharmacology and Toxicology 2020Quote: ... 1 μg/mL anti-CD71 APC (clone R17217) (eBioscience, San Diego, CA) and 2 μg/mL anti-TER119 PE-Cyanine7 (BioLegend, San Diego, CA). The cells were stained on ice for 30 min ...
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bioRxiv - Cancer Biology 2022Quote: CAR-T cells were co-cultured with tumor at ratio of 2:1 for 6 hours at 37o C in CO2 incubator with CD107a antibody (Biolegend, 328620, clone-H4A3) and golgistop (BD Biosciences ...
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bioRxiv - Immunology 2024Quote: ... Each patient was also stained with one of TotalSeq™-C0251/2/3/4 Antibody (Biolegend, Cat: 394661/3/5/7, 1:100) for sample multiplexing ...
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bioRxiv - Bioengineering 2023Quote: ... PBMCs were collected and stained using Brilliant Violet 711 anti-CD4 and APC anti-CD8 (Supporting Information Table 2) (Biolegend, San Diego CA). PBMCs were first washed and stained with Zombie Yellow (Supporting Information Table 2 ...
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bioRxiv - Microbiology 2023Quote: ... 2 million splenocytes were resuspended in 50 µl of fluorescence-activated cell sorter (FACS) buffer (PBS with 2% fetal bovine serum) and blocked with TruStain fcX (clone 93; BioLegend, San Diego, CA). The cells were washed and stained to identify B cell subsets with fluorophore-conjugated antibodies against CD45.2 (clone 104) ...
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bioRxiv - Microbiology 2023Quote: 2 × 106 single cell suspensions prepared from the lung or spleen were resuspended in 200 ul of PBS with 2% fetal bovine serum and blocked with TruStain fcX (BioLegend, San Diego, CA). T cell subsets were identified with antibodies against CD45 (clone 30-F11 ...
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bioRxiv - Immunology 2024Quote: ... Splenocytes and ascitic cells were isolated and re-stimulated with SIINFEKL peptide (immunodominant ovalbumin peptide) for 12 hours in the presence of 2 uM Monensin (BioLegend, San Diego, CA) with the following intracellular staining of IFN-γ and measurement of IFN-γ-producing CD8+ T cells (meaning ovalbumin-specific T cells ...
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bioRxiv - Immunology 2021Quote: ... enriched with the MojoSort Mouse CD4 Naïve T Cell Isolation Kit (480040 BioLegend) and were purified by flow-cytometry ...
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bioRxiv - Immunology 2021Quote: ... washed then stained with a viability dye (Zombie Aqua Fixable Viability Kit, Biolegend) followed by surface staining ...
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bioRxiv - Immunology 2019Quote: ... Annexin V staining was performed using an APC Annexin V staining kit (BioLegend) according to manufacturer’s instructions.
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bioRxiv - Immunology 2019Quote: ... CD4+ cells were first enriched using Mouse CD4 T Cell Isolation Kit (Biolegend) before electronic sorting ...
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bioRxiv - Immunology 2021Quote: ... Cell viability was assessed using the Zombie NIR™ Fixable Viability Kit (Biolegend) as per the manufacturer’s guidelines ...
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bioRxiv - Neuroscience 2021Quote: ... Primary CD14+ monocytes were isolated using Biolegend MojoSort magnetic isolation kit (Biolegend, 480094) per manufacturer’s instructions ...
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bioRxiv - Microbiology 2021Quote: ... Live/dead (LD) cells were identified with Zombie aqua fixable viability kit (Biolegend). CD3 ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were harvested and stained with Live/Dead Aqua staining kit from BioLegend as per manufacturer’s protocol ...
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bioRxiv - Neuroscience 2021Quote: The cells were stained with Zombie Green™ Fixable Viability Kit (BioLegend, USA) in PBS for 15 min at RT and in the dark using a dilution of 1:500 ...
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bioRxiv - Cell Biology 2021Quote: ... they were stained with Zombie Violet™ Fixable Viability Kit (1:1000, BioLegend) for 30 minutes on ice during secondary antibody staining ...
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bioRxiv - Immunology 2022Quote: ... naïve CD4+ or naive CD8+ T cell enrichment kit (Biolegend, San Diego, CA). Cell purity for all enrichments ranged between 90-98% ...
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bioRxiv - Developmental Biology 2022Quote: ... were incubated with Zombie Aqua™ Fixable Viability Kit (Biolegend, #423101, 1:1000) for 15 min at room temperature ...
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bioRxiv - Cancer Biology 2022Quote: ... and stained with fluorescent antibodies: APC-conjugated anti-mouse CD117 (c-Kit) (Biolegend) and PE-Cy7-conjugated anti-mouse Ly-6A/E (Sca-1 ...
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bioRxiv - Immunology 2021Quote: ... Intracellular staining was performed with the True Nuclear Transcription Factor staining kit (Biolegend) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2020Quote: ... TILs and N-TILs were stained using Zombie Aqua fixable viability kit (Biolegend), following which surface staining was performed with a mixture of fluorescence-conjugated antibodies (BD Biosciences or BioLegend) ...
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bioRxiv - Developmental Biology 2019Quote: ... cells were surface stained with the Zombie Aqua Fixable Viability Kit (Biolegend, #423101) for 0.5hr at RT,1:1000 dilution ...
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bioRxiv - Neuroscience 2021Quote: The LEGENDplexTM Human Inflammation (#740809) and Growth Factor (#740180) kits (BioLegend, CA, USA) were used to detect cytokines and growth factors in conditioned media from 3D MDMi and ReNcell VM mono- and co-cultures ...
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bioRxiv - Immunology 2021Quote: ... were stained for live/dead dye (Zombie Aqua fixable viability kit, #423102 BioLegend) in PBS ...
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bioRxiv - Cell Biology 2021Quote: ... The green dye CFSE Cell Division Tracker Kit (BioLegend, San Diego, California, USA) and DAPI (Sigma-Aldrich ...
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bioRxiv - Cell Biology 2021Quote: Moncytes were isolated by MojoSort CD14 positive selection kits (Biolegend, California, United States) and LS columns (Miltenyi Biotec ...
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bioRxiv - Immunology 2021Quote: ... or MojoSort™ Mouse CD8 T Cell Isolation Kit (BioLegend, San Diego, CA). Purity of the isolate CD8+ cells constantly exceeded 95%.
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bioRxiv - Immunology 2021Quote: The following antibodies and kits were purchased from Biolegend (San Diego, CA, USA): APC/Cy7 anti-CD4 (clone GK1.5) ...
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bioRxiv - Immunology 2021Quote: ... the thymocytes were fixed and permeabilized using a Fixation/Permeabilization Solution Kit (Biolegend), followed by staining using indicated antibodies ...
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bioRxiv - Immunology 2020Quote: ... cells were washed and stained with Zombie NIR™ Fixable Viability Kit (BioLegend) to label dead cells ...
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bioRxiv - Immunology 2020Quote: ... Dead cells were excluded using Zombie Aqua Fixable Viability Kit (BioLegend, Cat: 423102). For intracellular cytokine stainings ...
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bioRxiv - Immunology 2021Quote: ... Dead cells were first excluded using Zombie Aqua™ Fixable Viability Kit (BioLegend). Cells were then incubated with fluorescence conjugated antibodies in HBSS containing 2% BCS at 4℃ for 30 minutes to stain surface markers ...
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bioRxiv - Immunology 2021Quote: ... Cells were then stained with Zombie NIR™ Fixable Viability Kit (Biolegend, CA). Prior to acquisition ...
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bioRxiv - Immunology 2021Quote: ... MojoSort mouse CD4 T cell isolation kit and SDF-1α were from BioLegend. Recombinant human VCAM-1-Fc ...
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bioRxiv - Immunology 2022Quote: ... BrdU analysis was performed utilizing the Phase-Flow kit per manufacturer’s instructions (BioLegend), with cells cultured in BrDU for 2 hours prior to staining ...
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bioRxiv - Physiology 2022Quote: ... and IL-13 using a custom LEGENDplex kit according to manufacturer’s instructions (Biolegend). Flow cytometry was performed using a BD Celesta flow cytometer in UCLA’s Broad Stem Cell Research Center Flow Cytometry Core facility.
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bioRxiv - Immunology 2022Quote: ... The cells were then fixed with the True-Nuclear Transcription Factor kit (Biolegend). After fixation and permeabilization ...
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bioRxiv - Molecular Biology 2024Quote: ... cells were incubated with Zombie NIR™ Fixable Viability Kit (Biolegend, Cat# 423105) for 30 min at 4 ℃ before cell surface staining ...
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bioRxiv - Cancer Biology 2023Quote: ... To exclude dead cells Zombie Aqua Fixable Viability Kit (#423102, 1:100, BioLegend) was added ...
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bioRxiv - Microbiology 2022Quote: ... and sCD40L were measured using the human B cell premix kit from Biolegend Legendplex™ using manufacturer instructions ...
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bioRxiv - Immunology 2023Quote: ... and stained with a viability dye (NIR Fixable Viability Kit (42301/5, BioLegend) or LIVE/DEAD Fixable Near-IR Dead Cell Stain Kit (L34975 ...
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bioRxiv - Immunology 2023Quote: ... Tissues were blocked for endogenous biotin using an avidin biotin blocking kit (BioLegend), according to the manufacturer’s instructions and incubated overnight at 40C with one of the following primary antibodies ...