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Citations for BioLegend :
2501 - 2550 of 2877 citations for 7 Chloro 5 methyl 1H pyrrolo 2 3 c pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... cells were blocked with 5% bovine serum albumin (BSA) and 5% goat serum for 1hr at RT and incubated with primary antibodies (anti-βIII-tubulin antibody [BioLegend]) overnight at 4°C ...
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bioRxiv - Bioengineering 2022Quote: ... at 0.5 μL/well for 5 hours and washed with PBS for detection of intracellular IFNγ using anti-hIFNγ APC antibody (Biolegend). The frequency of IFNγ positive cells was analyzed by flow cytometry (CytoFLEX II ...
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bioRxiv - Biochemistry 2022Quote: ... neutrophils (1×106 cells/mL) were added to each well in the presence of APC conjugated anti-CD11b antibody CBRM1/5 (BioLegend) at 1 μg/mL ...
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bioRxiv - Immunology 2020Quote: Cytokine concentrations in culture supernatant or mouse plasma were measured with the LegendPLEX Inflammation Panel (13-plex) or the Mouse Th1 Panel (5-plex) per the manufacturer protocols (BioLegend). Quantification was done using LegendPLEX software v8.0 for Windows ...
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bioRxiv - Immunology 2020Quote: ... CD4+CD25high Treg cells were enriched from PBMCs after staining with PE-cyanine 5 (PE-Cy5)-labeled anti-CD25 Ab (BioLegend), followed by positive selection using anti-PE and anti-Cy5 microbeads (Miltenyi Biotec) ...
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bioRxiv - Immunology 2022Quote: Splenic T-cells from Ldlr-/- and T-AbcdkoLdlr-/- mice were isolated as described above and stimulated with 5 μg/mL plate-bound αCD3 (Biolegend) and 100 U/mL IL-2 (Peprotech ...
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bioRxiv - Immunology 2022Quote: Splenic T-cells from Ldlr-/- and T-AbcdkoLdlr-/- mice were isolated as described above and stimulated with 5 μg/mL plate-bound αCD3 (Biolegend) and 100 U/mL IL-2 (Peprotech ...
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bioRxiv - Immunology 2022Quote: ... 5×104 Tconv (CD4+CD25-) cells from TFAM control were labeled with CFSE Cell Division Tracker Kit (Cat# 423801, BioLegend) and cultured with mitomycin-treated wild type splenocytes ...
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bioRxiv - Cell Biology 2019Quote: Pellet cells obtained from BAL (n= 5 mice/group in three different experiment) were incubated with a mix of conjugated antibodies (Biolegend) for the following T-cell subset superficial markers ...
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bioRxiv - Cancer Biology 2019Quote: ... approximately 1 * 106 cells from dLN and 5 × 106 tumor cells were first stained with Zombie Red Fixable Viability Kit (BioLegend) for 30’ at room temperature and then incubated on ice for 15’ with an anti-mouse CD16/32 antibody (BioLegend) ...
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bioRxiv - Immunology 2019Quote: ... isolated lymphocytes were first restimulated with 5 ng/ml phorbal 12-myristate 13-acetate (PMA) and 500 ng/ml ionomycin in the presence of monensin (Biolegend) for 3.5 hours ...
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bioRxiv - Neuroscience 2020Quote: ... Sections were incubated overnight at 4°C in primary antibodies diluted in 1-5% serum in PBS-T according to the following concentrations: novel anti-parkin mAbs from Biolegend clones D (BioLegend ...
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bioRxiv - Cancer Biology 2020Quote: ... Single tumor cells were resuspended to 5 × 107 cell/mL and stained with CD298-APC (10 µL/106 cells)(BioLegend) and Sytox Green (ThermoFisher ...
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bioRxiv - Cell Biology 2020Quote: ... The virus particles were stained at a concentration of 5×108 particles mL−1 and labeled with anti-GFP PE antibody (Clone FM264G, BioLegend) at 0.4 μg mL−1 in a 100 μL staining volume for a minimum of 30 minutes at room temperature ...
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bioRxiv - Immunology 2020Quote: ... Splenocytes at (5 × 104 per well) were incubated with PMA (0.01 μM) /Ionomycin (0.167 μM) cocktail (BioLegend, San Diego, CA) as a positive control ...
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bioRxiv - Immunology 2021Quote: ... T-25 or T-75 flasks were coated with 5 µg/mL anti-CD3ε antibodies (clone 145-2C11; 100340, BioLegend) in phosphate-buffered saline (PBS ...
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bioRxiv - Immunology 2021Quote: ... Splenocytes from uninfected or infected mice were incubated at 37C° in 5% CO2 for 4 h in medium containing Monensin and Brefeldin A solutions (Biolegend). Cells were then stained for surface antigens (TCRβ-PacificBlue and NK1.1-PE) ...
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bioRxiv - Immunology 2021Quote: ... were coated overnight at 4°C with 50 µL/well of either 10 µg/mL (flat-bottom) or 5 µg/mL (round-bottom) anti-mouse CD3ε (BioLegend) in PBS (for stimulated wells) ...
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bioRxiv - Microbiology 2021Quote: ... for 15 min at 4°C prior to wash in 1 ml PBS and centrifugation at 1600 rpm for 5 min and surface molecule staining with CD206 APCCy7 (BioLegend), and intracellular staining of anti-human Arginase1 FITC (Ref ...
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bioRxiv - Immunology 2020Quote: ... for 5 minutes at room temperature prior to staining with a surface-stain cocktail containing the following antibodies purchased from BioLegend® (San Diego ...
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bioRxiv - Immunology 2021Quote: ... Cells were then blocked with human Fc-block (Miltenyi) in the presence or absence of purified anti-CD36 antibody (Clone 5-271; Biolegend) at 10 μg/ml for a further 15 min at 4°C ...
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bioRxiv - Cancer Biology 2022Quote: ... 5-10 x 106 cells were washed with PBS and stained with Zombie NIR Fixable live/dead dye (Biolegend, 423106) at 4 °C for 20 min ...
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bioRxiv - Immunology 2022Quote: ... 50,000 CD4+ T cells were added to each well of mBECs in 12-well chamber slides in Hank’s balanced salt solution (HBSS) with 1% FBS supplemented with 5 nM CXCL10 (Biolegend 573604) or vehicle (PBS) ...
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bioRxiv - Immunology 2022Quote: ... isolated lymphocytes were first restimulated with 5 ng/mL phorbal 12-myristate 13-acetate and 500 ng/mL ionomycin in the presence of monensin (Biolegend) for 3.5 h ...
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bioRxiv - Immunology 2022Quote: ... 5×104 B cells were first incubated with 5 µg/ml anti-IgM complexes and 1 µg/ml recombinant Human CD40L (Biolegend) for 30 mins and then 1×104 Tfr cells were added ...
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bioRxiv - Immunology 2024Quote: ... Mice CD4+ T cells (4x105/well in 96-well plate) were activated with plate-coated αCD3 (5 µg/ml, Biolegend) and αCD28 (10 µg/ml ...
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bioRxiv - Cell Biology 2024Quote: ... Cells were immunolabeled in blocking solution for 1 hour at RT with antibodies against the following proteins: ALIX at 5 μg/ml (mouse; no. 634502, Biolegend); IST1 at 0.4 μg/ml (rabbit ...
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bioRxiv - Immunology 2024Quote: ... isolated naïve splenic B cells were cultured at a density of 0,5 x 106 cells/ml in the presence of 25 μg/ml LPS and 5 ng/ml IL-4 and stained with anti-CD138 (BioLegend). B cell proliferation was assessed by CellTrace Violet (Thermofisher ...
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bioRxiv - Immunology 2024Quote: ... for 40 min on ice or with biotinylated anti-MR1 antibody 26.5 (BioLegend, custom conjugation, used at 5 μg/ml) for 40 minutes on ice ...
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bioRxiv - Immunology 2024Quote: ... Plates were washed 5 times in 150 µL of PBST and incubated with 100 µL of streptavidin conjugated to horseradish peroxidase (HRP, BioLegend) diluted 1:2000 in PBS for 1 hour at RT ...
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bioRxiv - Immunology 2024Quote: ... Wells were washed 5 times with 200 µL PBST and streptavidin-conjugated to horseradish peroxidase was added (1:2000 in PBS, BioLegend) for 30 minutes at RT ...
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bioRxiv - Immunology 2024Quote: ... in the presence of S2 mAbs (5 μg/mL), protein transport inhibitor cocktail (1:500, eBioscience) and anti-CD107a APC (clone H4A3, BioLegend). Antibody-dependent NK cell activation was allowed to occur for 4 hours at 37°C and 5% CO2 ...
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bioRxiv - Bioengineering 2024Quote: T-cells were cultured in RPMI-1640 media supplemented with 5 µg/mL brefeldin A (BioLegend, San Diego, CA, USA). After 24 hours of cultivation ...
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bioRxiv - Immunology 2023Quote: For Langerhans cells analysis, after centrifugation (450g, 5 min) cells were stained with anti-CD45 FITC (BioLegend, clone 30-F11), anti-EpCAM APCFire750 (BioLegend ...
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bioRxiv - Cancer Biology 2023Quote: ... Pospho-GSK-3α/β and pospho-Akt were quantitated following 5 minutes of stimulation in transfected T cells fixed and permeabilized with fixation and permeabilization buffers (Biolegend) and stained with anti-pospho-GSK-3α/β (R&D systems ...
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bioRxiv - Genomics 2023Quote: ... Cells were pre-incubated with 5 µl of Human TruStain FcX™ (Fc Receptor Blocking Solution, BioLegend Cat. No. 422301) per 100µl of cell suspension for 5–10 minutes at room temperature ...
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bioRxiv - Immunology 2023Quote: ... anti-CD25(Percp-Cy5.5, #302626), anti-CD69(APC, #310910), anti-mouse CD25(PE, #12-0251-83), anti-mouse CD69(FITC, #104506) from Biolegend, anti-human IgG(SureLight® PE ...
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bioRxiv - Cancer Biology 2023Quote: ... CD8+ T-cells were plated in the presence of 5 μg mL−1 plate-bound anti-CD3ε (clone145-2C11, BioLegend) and 1 μg mL−1 soluble anti-CD28 (eBioscience ...
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bioRxiv - Biochemistry 2023Quote: ... Membranes were incubated one hour in 5% milk before adding the primary antibody (anti-HA 3F10, Roche 27573500, 1:2500, anti-Ubiquitin P4D1, Biolegend, 1:10,000 ...
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bioRxiv - Cancer Biology 2023Quote: ... 100 000-200 000 cells per sample were processed by blocking with 5 µl of Human TruStain FcX Blocking Solution (BioLegend) for 10 min at 4°C ...
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bioRxiv - Pathology 2023Quote: ... Ldlr−/− mice fed a chow diet or HC diet for 4 days were anesthetized using Isoflurane and injected intravenously with 5 μg anti-mouse TIM4-APC antibody (RMT4-54, BioLegend). They were then euthanized 5 minutes after injection by cervical dislocation and perfused through the portal vein with PBS and then with 4% ice-cold PFA ...
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bioRxiv - Immunology 2023Quote: ... lysis was performed at room temperature for 5 minutes in 1X RBC lysis buffer (10X RBC lysis buffer diluted in mqH2O, 420301, BioLegend) and reaction was stopped by diluting the 1X RBC lysis buffer with 35ml 1X DPBS ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were washed once with FACS buffer and pre-incubated with 5 μg/mL TruStain FcX anti-mouse CD16/32 antibody (clone 93, BioLegend) in 100 μL FACS buffer for 5 minutes on ice before immunostaining ...
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bioRxiv - Cancer Biology 2024Quote: 1 × 106 target cells (B16-F10 and Hepa1-6) were suspended in 1ml DPBS containing 5 µM Calcein-AM (BioLegend) and incubated at 37℃ for 30 minutes in the dark ...
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bioRxiv - Cancer Biology 2024Quote: ... PCLS were stained in 250 μl of 5 μg/ml Hoechst 33342 and 2.5 μg/ml AlexFluor 647-conjugated anti-EpCAM (Biolegend 118212) by incubating for 1 hr at 37°C ...
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bioRxiv - Immunology 2024Quote: ... 0.5-1×106 cells were resuspended in 50 μL/well T cell stimulation media (5 μg/mL anti-mouse CD28 (Biolegend), 5 ug/mL BFA) ...
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bioRxiv - Immunology 2024Quote: ... T cell stimulations were performed by first coating a 96-well plate with 5 μg/mL anti-mouse CD3 (Biolegend) in 50 μL/well PBS overnight the day before tissue harvest ...
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bioRxiv - Immunology 2024Quote: ... and 5 µg/mL of soluble Ultra-LEAF™ purified anti-human CD28 monoclonal antibody (clone 28.2, BioLegend, cat. 302933) in R10 medium [RPMI-1640 medium supplemented with 10% heat-inactivated FBS ...
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bioRxiv - Bioengineering 2024Quote: ... for 5 min on ice and then stained with fluorochrome conjugated antibodies: CD19 (PerCP-Cy5.5, clone: 1D3, BioLegend, cat: 152406) CD95 (PE-Cy7 ...
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bioRxiv - Neuroscience 2020Quote: ... the neurons were fixed and stained as previously described38 using primary antibodies for β-3-Tubulin (TUJ1; 1:200; BioLegend, San Diego, CA, USA; 801201) and TH (1:300 ...