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Citations for BioLegend :
2351 - 2400 of 2877 citations for 7 Chloro 5 methyl 1H pyrrolo 2 3 c pyridine since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Bioengineering 2021Quote: ... T cells were similarly isolated and stained with Ghost Dye and lineage/differentiation markers (without CD19) and with TIM-3 BV711 (Biolegend clone F38-2E2), LAG-3 BV421 (Biolegend clone 11C3C65) ...
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bioRxiv - Cancer Biology 2024Quote: ... Spleenocytes were resuspended in 3 ml MojoSort™ buffer and enriched for CD8+ T cells using the MojoSort™ Mouse CD8 T Cell Isolation Kit (BioLegend, 480035), following manufactureŕs instructions.
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bioRxiv - Microbiology 2024Quote: ... IL-10, IL-12p70, IFN-α2, IFN-β, IFN-λ1, IFN-λ2/3, IFN-γ, TNF-α, IP-10 and GM-CSF; BioLegend) assay was performed on patient plasma samples as per the manufacturer’s instructions.
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bioRxiv - Immunology 2021Quote: Isolated cells were stained at 4°C in FACS buffer (PBS supplemented with 2.5% bovine serum albumin) with and hematopoietic lineage markers including Ly6G (BioLegend, clone 1A8, 1:600), CD11b (BioLegend ...
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bioRxiv - Molecular Biology 2021Quote: ... blotted membranes were blocked with 5% milk in TBST for 30 minutes and probed overnight (4°C) with primary antibody (anti-β-actin [Sigma-Aldrich, A5441], anti-HA [Biolegend, 901501]). After 3 washes with TBST ...
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bioRxiv - Evolutionary Biology 2022Quote: ... PE-Cy7 conjugated anti-rat CD11b/c (clone OX-42, Cat#201818), APC-Cy7 conjugated anti-rat CD4 (clone W3/25, Cat#201518) from Biolegend (San Diego, USA), and AF647-conjugated anti-rat RT1B (clone OX-6 ...
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bioRxiv - Neuroscience 2020Quote: ... 0.05% Sodium Azide in PBS) and incubate overnight at 4°C with the monoclonal anti-amyloid beta antibody 6E10 (Biolegend, 803001, lot. B228658) at a concentration of 3μg/ml (Antibody buffer ...
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bioRxiv - Cancer Biology 2021Quote: ... Cells were stained for 30 min at +4°C with APC or PE-conjugated anti-CD133 antibodies (diluted at 1/200 or 1/400 respectively; Biolegend, San Diego, USA) or corresponding control Immunoglobulin G1 (IgG1 ...
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bioRxiv - Immunology 2022Quote: ... This is followed by secondary staining for 30 min at 4°C with APC-conjugated mouse anti-His antibody (BioLegend, #362605, 1:50) or isotype control ...
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bioRxiv - Immunology 2019Quote: ... slides were incubated for 50 min at 25 °C with different combinations of the following anti-mouse Abs (Thermo Fisher Scientific, Biolegend, BD Biosciences): Alexa Fluor 488-labeled anti-CD3 (HM3420 ...
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bioRxiv - Systems Biology 2019Quote: ... We washed the samples again with 0.1% BSA-PBS and then incubated for 10 minutes at 4°C with 1:250 anti-NGFR antibody conjugated directly to PE-Cy7 (Biolegend, 400126, clone ME20.4) in 0.5% BSA-PBS with 2mM EDTA ...
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bioRxiv - Immunology 2019Quote: ... Cell suspensions were incubated for 10 minutes at 4°C with 10μg/ml Fc Block (purified rat anti-mouse CD16/CD32, Biolegend TruStain FcX anti-mouse) to block non-specific binding of antibodies to Fc Receptors and then incubated at 4°C for 30 minutes with anti-CD45-PE (1:300 ...
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bioRxiv - Immunology 2019Quote: Cells were incubated for 10 minutes at 4°C with 10μg/ml of purified rat anti-mouse CD16/CD32 (Biolegend TruStain FcX anti-mouse) to block non-specific binding of antibodies to Fc Receptors and then incubated at 4°C for 30 minutes with Fixable Viability Staining e780 and fluorochrome/biotin conjugated antibodies (purchased from BD Bioscience ...
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bioRxiv - Pathology 2019Quote: ... The ears were fixed in 4% paraformaldehyde for 20 minutes at 4°C and incubated overnight in 0.05% Triton X-100 in PBS containing anti-human CD31 (Biolegend, San Diego, CA, USA) antibody conjugated to either PE or AF647 in a 1:200 dilution ...
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bioRxiv - Neuroscience 2019Quote: ... The triangularis sterni muscle was dissected and incubated overnight (4°C) in anti-βIII-tubulin antibody conjugated to Alexa488 (BioLegend 801203; 1:200) and Alexa594-conjugated α-bungarotoxin (Invitrogen B13423 ...
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bioRxiv - Cell Biology 2021Quote: ... Cells were incubated for 30 min at 4°C with pre-conjugated fluorescent labeled antibodies with the following combinations: CD45 (BioLegend, 368532 (PE/Cyanine7)) ...
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bioRxiv - Immunology 2021Quote: ... 1 million cells pooled from several individuals were stained with TotalSeq-C custom human panel together with Human TruStain FcX (Biolegend. Cat. No. 422301) on ice for 30 minutes ...
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bioRxiv - Immunology 2020Quote: ... and then incubated in the dark for 30 min at 4°C: Myeloid-lymphocyte common lineage panel – TruStain FcX (anti-CD16/32) (clone: 93; dilution: 1:100, BioLegend, San Diego, CA), CD45-Pacific Blue (clone ...
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bioRxiv - Genomics 2021Quote: ... Sections were incubated with the following primary antibodies at the indicated dilution in blocking buffer overnight at 4°C: NeuN (BioLegend 1B7, cat #834502), p65 (BioLegend Poly6226 ...
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bioRxiv - Immunology 2021Quote: ... Spike and RBD tetramers were synthesized by incubating biotinylated protein with fluorophore-conjugated streptavidin overnight at 4°C (streptavidin-PE, Tonbo, cat. no. 50-4317-U100, streptavidin-APC, Tonbo, cat. no. 20-4317-U100, streptavidin-BV421, BioLegend, cat. no. 405226) at a molar ratio of 5:1 ...
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bioRxiv - Bioengineering 2024Quote: ... Sections were then stained overnight at 4 °C in a humidified chamber with mouse monoclonal anti-human CD68 (hCD68, 1:400, BioLegend, clone Y1/82A) and rabbit polyclonal anti-mouse IL-10 (mIL-10 ...
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bioRxiv - Molecular Biology 2024Quote: ... according to manufacturer’s recommendations and stained for 1 hour at 4°C with the following antibodies: NGFR (345110; 1:600, BioLegend, San Diego, CA, USA), α-tubulin (NB100-69AF405 ...
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bioRxiv - Immunology 2024Quote: ... and incubated at 37°C for 30 min with either a 1/500 dilution of PE-labeled anti-6xHis tag mouse MAb (Biolegend, San Diego, CA) to detect VHHs or Alexa Fluor 647-labeled goat anti-human IgG (H+L ...
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bioRxiv - Immunology 2023Quote: CD3+ T cells were purified from BALB/c mouse spleen cells with the MojoSort mouse CD3 T cell isolation kit (Biolegend, San Diego, CA) according to the manufacturer’s instructions ...
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Cell therapy with IL-10-producing group 2 innate lymphoid cells suppresses Graft-versus-Host diseasebioRxiv - Immunology 2023Quote: ... Cells were incubated with a TotalSeq-C antibody cocktail as well as hashtag antibodies to allow for post sequencing differentiation of distinct expanded ILC subsets(BioLegend, Supplemental Table 1) for 30min ...
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bioRxiv - Immunology 2023Quote: ... prior to the end of the CD45- sort one vial of TotalSeq-C Human Universal Antibody Cocktail v1.0 (Biolegend, San Diego, CA, USA) was equilibrated to room temperature for 5min and then spun at 10,000g for 30 seconds ...
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bioRxiv - Immunology 2024Quote: ... Surface proteins were then stained at 4°C for 30 minutes using the following markers: NIR Zombie dye APC-Cy7 (Biolegend, Cat No. 423105), CD20 PE (Biolegend ...
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bioRxiv - Immunology 2023Quote: ... peripheral blood or BM were incubated for 20 min at 4°C with the following anti-mouse conjugated antibodies: anti-CD45-PerCP/Cy5.5 (BioLegend, clone 30-F11, #103132), anti-CD45.1-PE/Cyanine7 (BD Biosciences ...
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bioRxiv - Biophysics 2023Quote: ... we incubate 5-6*105 NK92 cells prior to mixing them into the collagen for one hour in serum-free cell culture medium at 37°C with or without the following antibodies: 10 μg/ml anti-integrin activating β1 (CD29) antibody (Biolegend, cat. no. 303002) and the corresponding IgG1 isotype (Biolegend ...
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bioRxiv - Cancer Biology 2023Quote: ... and stained overnight at 4°C in blocking solution with the following primary antibodies and dilutions: anti-Nestin (1:150, Biolegend, #656802, clone 10C2), anti-EGFR (1:300 ...
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bioRxiv - Bioengineering 2024Quote: ... Sections were then stained overnight at 4 °C in a humidified chamber with rat monoclonal anti-mouse CD68 (1:400, BioLegend, clone FA-11) and rabbit polyclonal anti-mouse IL-10 (1:25 ...
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bioRxiv - Immunology 2021Quote: ... lymph node cell IL-1β/IL-23 plus spermidine treatment group (LN sper) with hashtag 5 (BioLegend, Cat# 155809). The five cell populations were mixed and barcoded ...
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bioRxiv - Molecular Biology 2021Quote: CH12 cells were stimulated to undergo CSR to IgA by treatment with 1-5 μg/mL α CD40 (BioLegend), 5 ng/ml TGF (R&D Systems ...
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bioRxiv - Microbiology 2022Quote: ... the cell culture media was replaced with complete Huh-7.5 media with 5 μg/mL BFA (Brefeldin A, Biolegend) or equal volumes of DMSO (vehicle control ...
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bioRxiv - Immunology 2021Quote: ... for 30 min, washed with FACS buffer (PBS, 5% BSA, 0.05% sodium azide) and stained with anti-mouse IgG (Poly4053; Biolegend), IgA (Clone 11-44-2 ...
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bioRxiv - Immunology 2019Quote: ... or with culture medium only in the presence of 5¼g/ml Brefeldin A (Biolegend, San Diego, CA, USA) before being submitted to the flow cytometry staining process.
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bioRxiv - Cancer Biology 2021Quote: ... Red blood cells were lysed (15mM NH4Cl + 10mM KHCO3 for 5 min at R.T.) and CD16/32 antibody was used to block the endogenous FC (#101301, Biolegend). Cells were analysed using a BD FACS LSR Fortessa ...
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bioRxiv - Immunology 2021Quote: ... the cells were incubated for 5 min on ice with Fc Block (TruStain fcX anti-mouse CD16/32, Biolegend) and washed with 200 μL FACS buffer ...
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bioRxiv - Microbiology 2021Quote: ... resuspended in 100 μl FACS Buffer plus 5 μl TruStain fcX™ (anti-mouse CD16/32) (Biolegend Cat#101320), and incubated for 10 min on ice ...
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bioRxiv - Microbiology 2021Quote: ... Unspecific bindings were blocked by addition of 5 μl TruStain fcX™ (anti-mouse CD16/32) (Biolegend, Cat#101320) and incubation for 10 min on ice ...
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bioRxiv - Biochemistry 2020Quote: ... Tetramers were prepared by mixing 1 or 5 pmol of biotinylated protein monomer with 0.25 to 1.25 pmol of streptavidin-PE (Biolegend #405245) respectively and incubating for 2 hours at room temperature ...
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bioRxiv - Cell Biology 2021Quote: ... NK cells were pre-incubated for 20 minutes with anti-CD56 Alexa Fluor 647 (HCD56, Biolegend, 5 μg/ml). For granule polarization assays ...
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bioRxiv - Cancer Biology 2022Quote: ... or TFH mimicking signals (including 0.02 μg/mL recombinant human IL-4, Peprotech; 0.05 μg/ml recombinant human IL-21, Peprotech, and 5 μg/mL anti-human CD40, Biolegend). Complete RPMI medium contained RPMI 1640 (Gibco ...
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bioRxiv - Immunology 2022Quote: ... PVDF membrane transferred proteins were probed with antibodies against anti-Siglec 5/14 (clone 1A5, Biolegend, San Diego, CA).
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bioRxiv - Microbiology 2020Quote: ... cells were counted and plated on 6 well plates in RPMI + 5% FBS + 20 ng/ml GM-CSF (BioLegend). Cells were maintained at 37° C 5% CO2 and supplemented on day 3 ...
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bioRxiv - Pathology 2022Quote: ... cell suspensions were centrifuged again at 300 g for 5 min and then preincubated with 2.5 µg/ml Fc blocker (156604 for mouse, 422302 for human, BioLegend) on ice for 10 min ...
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bioRxiv - Neuroscience 2022Quote: ... Primary antibodies were: Syn303 [a mouse monoclonal antibody that recognizes residues 1-5 of human α-synuclein (50)] (BioLegend) at 1:4,000 ...
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bioRxiv - Biochemistry 2022Quote: ... per reaction were incubated with 200 μl antibody binding buffer [1X PBS/ 0.02% Tween 20] and 5 μg of anti-FMRP (Biolegend) and anti-FXR1(Santa Cruz Biotechnology #sc-374148) ...
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bioRxiv - Bioengineering 2019Quote: ... Cells were washed with 1X PBS + 5% FBS + 0.1% sodium azide before being incubated for 20 minutes with Zombie Aqua viability stain (Biolegend) at room temperature ...
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bioRxiv - Immunology 2020Quote: ... Th1 cells were differentiated for 4 days in the presence of plate-bound αCD3 (5 μg/ml; Biolegend, #100339), rIL-12 (25 ng/ml ...