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Citations for BioLegend :
2451 - 2500 of 3473 citations for PLGF 2 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2024Quote: ... Brefeldin A (5 μg/ml) and Monensin (2 μM) (BioLegend) were added to the cultures during the last 4 h of incubation to allow intracellular cytokine accumulation ...
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bioRxiv - Immunology 2022Quote: ... and the CD137 APC antibody (2:100, Biolegend, Cat# 309810) for an additional 4-hour incubation ...
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bioRxiv - Immunology 2022Quote: ... 2 mM EDTA and APC anti-mouse CD274 (Biolegend, 124312,) at 4°C for 30 min ...
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bioRxiv - Cancer Biology 2023Quote: ... anti-ERK1/2 phospho(Thr202/Tyr204) antibody (clone: 6B8B69, BioLegend); and anti-Actin Ab (clone ...
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bioRxiv - Immunology 2023Quote: ... IL-2 (PE/BV421, Biolegend, 1:50, clone JES6-5H4), PDCA-1 (Pacific Blue ...
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bioRxiv - Immunology 2023Quote: ... Mouse IgG1 and IgG2b isotypes (2 μg/ml, each; Biolegend) were used as isotype controls for primary antibody staining ...
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bioRxiv - Immunology 2023Quote: ... anti-mouse IgG1 (Biolegend; RMG1-1; at 2 μg/ml), antimouse IgG2b (BD Biosciences ...
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bioRxiv - Microbiology 2023Quote: ... IL-2 of supernatants were analyzed by ELISA (BioLegend #431004).
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bioRxiv - Immunology 2023Quote: ... in the presence of 20 ng/mL rmIL-2 (Biolegend), 5 ng/mL m/h rTGF-β (eBioscience ...
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bioRxiv - Bioengineering 2023Quote: ... which comprised 1.5 μg of mouse recombinant IL-2 (Biolegend) and 50 μg of anti-IL-2 monoclonal antibodies (S4B6 ...
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bioRxiv - Immunology 2023Quote: ... and biotin anti-mouse IL-2 (clone JES6-5H4, BioLegend) antibody was used as the secondary antibody ...
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bioRxiv - Cell Biology 2023Quote: ... CD18 (clone CBR LFA-1/2, AF647, Biolegend, 1:100), and CD29 (clone TS2/16 ...
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bioRxiv - Immunology 2024Quote: ... TotalSeq™-A0302 anti-mouse Hashtag 2 Antibody (Biolegend, 155803), and TotalSeq™-A0303 anti-mouse Hashtag 3 Antibody (Biolegend ...
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bioRxiv - Immunology 2024Quote: ... anti-IgD-PerCP-Cy5.5 (clone IA6-2, BioLegend, 1:50), anti-CD3-FITC (clone HIT3a ...
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bioRxiv - Cancer Biology 2021Quote: ... The following antibodies were used: Alexa Fluor® 488 anti-human CD45 antibody (clone H130, catalog #304019, BioLegend); APC anti-human CD3 antibody (clone HIT3a ...
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bioRxiv - Systems Biology 2020Quote: ... respective concentrations (ranging from 25 ng/mL to 100 ng/mL) of recombinant human IL-8 (Biolegend, 715404) and/or recombinant human CCL2 (Peprotech ...
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bioRxiv - Developmental Biology 2021Quote: ... The following antibodies and dilutions were used: anti-human CD172a/b (SIRPα/β) (Biolegend; clone SE5A5; 1:200) anti-human CD13 (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... B cells were washed with FACS buffer and stained with PerCP-cy5.5 anti-human CD19 antibody (Biolegend, 363016), FITC anti-human CD3 antibody (Biolegend ...
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bioRxiv - Immunology 2021Quote: IL-21 concentration from tonsil mono-cultures was measured using Human IL-21 ELISA MAX Deluxe kit (Biolegend) according to manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... for 1 hour and subsequently stained overnight at 4°C with mouse anti-human TCR Vα7.2-PE (Clone 3C10, Biolegend), mouse anti-human CD161-AF488 (Clone NKR-P1A ...
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bioRxiv - Bioengineering 2019Quote: ... Samples were thawed on ice prior to detection and analysis using LEGENDplex human hematopoietic stem cell panel (BioLegend), according to manufacturer’s protocol.
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bioRxiv - Immunology 2020Quote: ... The following antibodies were used for flow cytometry staining: APC/Cy7 anti-human CD3 Antibody (clone HIT3a, Biolegend), Brilliant Violet 605™ anti-human CD56 (NCAM ...
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bioRxiv - Cancer Biology 2019Quote: ... IL-6 levels of culture supernatants were determined using the LEGENDplex™ Human Inflammation Panel I (BioLegend, 740809), according to the manufacturer’s instructions ...
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bioRxiv - Immunology 2021Quote: ... and the production of IFN-γ and Granzyme B was checked with PerCP anti-human IFN-γ (BioLegend) and FITC anti-human Granzyme B (BioLegend ...
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bioRxiv - Biochemistry 2020Quote: ... bound proteins were stained for 30 minutes with anti-human IgG-APC (clone HP6017, 1/250 dilution; BioLegend) and anti-HIS-FITC (chicken polyclonal ...
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bioRxiv - Cancer Biology 2020Quote: ... cells were stained with an anti-human CD271(ΔNGFR) PE-conjugated antibody (BioLegend, Cat. No. 345106, RRID: AB_2152647) (dilution 1:200 ...
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bioRxiv - Neuroscience 2020Quote: ... cells were incubated with a mouse anti-human C3 APC conjugated antibody (1:50; Biolegend, 846106, clone 3E7iC3b) and mouse anti-Myc Alexa488 conjugated antibody (1:50 ...
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bioRxiv - Microbiology 2020Quote: ... the purity of the monocyte/macrophage population was checked by staining with FITC anti-human CD14 antibody (BioLegend) and FC and used for infection.
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bioRxiv - Cancer Biology 2022Quote: The cells used in this study were labeled with anti-human B7-H3 monoclonal antibody (Biolegend, clone MIH42) PerCP/Cyanine 5.5 conjugate at 4°C for 45 minutes in the dark ...
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bioRxiv - Cancer Biology 2022Quote: ... blocked in 1X PBS containing 1 mg/ml BSA and 2.5 µl of human FcX-TruStain (BioLegend Inc.) for 15 minutes and incubated with PE-c-CASPASE3 antibody (Cell Signaling #9978 ...
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bioRxiv - Immunology 2022Quote: ... Cells were washed twice after the primary stain and incubated with PE–anti-human Fc antibody (Biolegend, 410708) in MACS buffer for 20 min on ice ...
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bioRxiv - Immunology 2022Quote: ... HIV-infected cells were previously incubated with 10 μg/ml of anti-human HLA-E (clone 3D12, Biolegend) antibody for 20 mins at RT ...
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bioRxiv - Immunology 2021Quote: ... The following antibodies were used in the flow panel: mouse anti-human CD4 Alexa Fluor 700 (OKT4, Biolegend), mouse anti-human CD20 BV785 (2H7 ...
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bioRxiv - Microbiology 2020Quote: ... the purity of the monocyte/macrophage population was checked by staining with FITC anti-human CD14 antibody (BioLegend) and FC ...
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bioRxiv - Immunology 2020Quote: ... frozen PBMC were thawed and stained with 3 μl of anti-human CD5-APCeF780 antibody (clone L17F12, Biolegend), 2 μl of anti-human CD19-PECy7 antibody (clone HIB19 ...
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bioRxiv - Immunology 2020Quote: ... Human trachea was stained for 72 hours in PBS serum and the following antibodies: hCD103-AlexaFluor 594 (Biolegend) 1:40 ...
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bioRxiv - Cancer Biology 2021Quote: ... The stained samples were then incubated and stained with Alexa Fluor® 700 anti-human CD45 Antibody (BioLegend). Post staining ...
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bioRxiv - Physiology 2022Quote: ... 3 106 cells were then incubated with Fc receptor blocking solution (Human Trustain FcX, Biolegend, San Diego, USA) for 5 minutes at 4°C ...
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bioRxiv - Neuroscience 2022Quote: Sections were stained with the primary antibodies: anti-human CD105 (endoglin, ENG) biotin-conjugated (1:100, BioLegend, 323214), anti-human CD31-Alexa Fluor® 594 conjugated (1:400 ...
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bioRxiv - Immunology 2019Quote: ... Co-culture supernatants were assayed in duplicate for IgG1 production using the Legendplex Human Immunoglobulin Isotyping Panel (Biolegend) and data acquired on a BD Symphony A5 cytometer ...
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bioRxiv - Immunology 2019Quote: The following fluorescent conjugated antibodies were used: antibodies to human CD3ε-BUV395 or PeCy7 (UCHT1; eBioscience and Biolegend), CD4-PerCPCy5.5 (RPA-T4) ...
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bioRxiv - Immunology 2019Quote: ... Cell surface phenotyping of transduced cells was determined by staining with BV785-conjugated anti-human CD3 (UCHT1, BioLegend), BV605-conjugated anti-human CD4 (OKT-4 ...
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bioRxiv - Immunology 2019Quote: ... an Fc block step was performed by resuspending cells in 50μl of Fc block prepared in PBS/5%BSA (room temperature) using 5μl of Human Trustain FcX (Biolegend) per sample i.e ...
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bioRxiv - Cell Biology 2019Quote: ... incubated in the following order: anti-mouse/human CD44 (1:100 dilution, 30 min incubation; Biolegend cat#103001), rabbit anti-rat AlexaFluor® 488 (1:1000 dilution ...
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bioRxiv - Immunology 2019Quote: ... Human MAIT cells were isolated by sorting CD2 MACS-enriched (Miltenyi) leukocytes with CD161 and Vα7.2 antibodies (BioLegend). MAIT cells were grown for around 6 weeks in complete RPMI media supplemented with IL-2 ...
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bioRxiv - Cancer Biology 2020Quote: ... resuspended in FACS buffer (1X PBS containing 0.5% FBS and 0.01% sodium azide) and used for staining with the following anti-human antibodies (Biolegend): CD45 ...
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bioRxiv - Cell Biology 2021Quote: Cells dissociated from primary HMEC strains (passage 4) were stained with anti-human CD271-PerCP/Cy5.5 (Biolegend #345122) and anti-human CD133-PE (Biolegend #372804 ...
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bioRxiv - Immunology 2021Quote: ... cells were also stained with Alexa Fluor 488 anti-human TCR α/β (306712, BioLegend, San Diego, CA).
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bioRxiv - Immunology 2021Quote: ... Murine monoclonal FITC anti-human CD11b (activated) antibody (IgG1, clone CBRM1/5) was from Biolegend (San Diego, CA). Goat anti-mouse (GαM ...
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bioRxiv - Immunology 2021Quote: ... In order to measure secreted IL-1β we used the commercial Human IL-1β ELISA MAX kit (Biolegend), according to the manufacturer’s instruction.