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Citations for BioLegend :
2401 - 2450 of 3473 citations for PLGF 2 Human HEK293 His since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Immunology 2023Quote: ... stable transfectants were screened by flow cytometry using mouse anti human PD1 (cat: 621610, BioLegend, CA, USA) and isotype control using allophycocyanin (APC)-conjugated mouse IgG1(cat ...
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bioRxiv - Immunology 2023Quote: Engineered T-cells were stained with increasing concentrations of AF647-conjugated anti-human αβTCR antibody (IP26: Biolegend) and HLA.A2/9V-AF647 for determination of label saturation concentration of CMV-specific RA14 TCR T-cells and HLA.A2/NY-ESO-1-specific engineered T-cells ...
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bioRxiv - Immunology 2023Quote: ... Target cell lines were stained using phycoerythrin (PE)-conjugated mouse anti-human PDL1 antibody (cat: 329706, BioLegend) and APC-conjugated mouse anti-human PDL2 antibody (cat ...
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bioRxiv - Cancer Biology 2023Quote: ... The pellet was resuspended in red blood cell lysis buffer (BioLegend, catalog no. 420301, all human samples) and incubated for 3 min on ice with periodic shaking ...
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bioRxiv - Immunology 2023Quote: ... supernatants from cell cultures were harvested for detecting cytokine using LEGENDplexTM Human Th1 Panel (5-Plex) (BioLegend). Samples were diluted 5-fold using assay buffer and then mixed with beads and shaken for 2 hours at 500 rpm in a 96 plate well ...
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bioRxiv - Genetics 2023Quote: ... splenic B cells were collected and stained with FITC anti-mouse/human GL7 (Biolegend, Cat. No. 144604) and PE hamster anti-mouse CD95 (BD ...
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bioRxiv - Cancer Biology 2023Quote: ... Fc-block was performed by incubating cells in a dilution of Human TruStain FcX (#422302, BioLegend, USA) and mouse TruStain FcX (#101320 ...
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bioRxiv - Microbiology 2023Quote: ... for 30 minutes at RT after adding Human TruStain FcX (1:200 dilution, Biolegend, San Diego, CA) for 5 minutes ...
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bioRxiv - Immunology 2023Quote: ... we used Ultra-LEAF™ purified anti-human TIM-3 antibody (10μg/ml, clone F38-2E2, Biolegend) or its respective isotype Ultra-LEAF™ purified mouse IgG1 antibody (10μg/ml ...
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bioRxiv - Immunology 2023Quote: ... ELISA for IFN-γ and IL-10 were also performed with human ELISA MAX Deluxe Sets (BioLegend) to verify the multiplex ELISA results ...
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bioRxiv - Cancer Biology 2023Quote: ... and Brilliant Violet 510™ anti-human CD45 (clone HI30, dilution 1/20, Biolegend, Amsterdam, The Netherlands) antibodies were used for progenitor cell subset phenotyping ...
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bioRxiv - Immunology 2023Quote: ... Commercial anti-RBD antibodies (mouse IgG1κ, clone 1035753, R&D systems or human IgG1κ, clone AM001414, BioLegend) and isotype controls (mouse IgG1κ ...
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bioRxiv - Neuroscience 2023Quote: ... Concentrations of chemokines in cell supernatants were measured using the LEGENDplexTM Human Proinflammatory Chemokine Panel 1 (Biolegend). Readings were made on an AttuneTM Nxt Flow Cytometer.
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bioRxiv - Immunology 2023Quote: ... Intracellular proteins were stained using the following antibodies: anti-human T-bet PE (1:20, 4B10, BioLegend), and anti-human Ki-67 BUV395 (1:50 ...
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bioRxiv - Genomics 2023Quote: ... The PBMC pellet was resuspended and cells were stained with Human TruStain FcX Fc Blocking Reagent (BioLegend) for 10 min at 4°C and then with fixable viability dye DAPI (Merck) ...
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bioRxiv - Cancer Biology 2023Quote: TCR-transduced cell lines were stained with anti-human TCR α/β (Clone IP26, BioLegend Cat. #306717) and anti-human CD3 antibody (Clone HIT3a ...
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bioRxiv - Cancer Biology 2024Quote: ... Samples were randomized on the assay plates and analyzed using the LEGENDplex human CD8/NK panel (BioLegend). Samples were read on a CytoFLEX S flow cytometer (Beckman Coulter) ...
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bioRxiv - Neuroscience 2024Quote: ... the iAstrocytes were stained with 1:50 PE anti-human HLA-A,B,C Antibody (BioLegend, 311406) and DAPI for 30 minutes at 4°C ...
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bioRxiv - Cancer Biology 2024Quote: ... or Anti-human/mouse CD49f antibody (#313620, Biolegend) (isotype control, Pacific Blue Rat IgG2a, k; #400527, Biolegend). For GD3 FACS analysis ...
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bioRxiv - Cancer Biology 2024Quote: ... a single-cell solution of lung cells was incubated with Human TruStain FcX (Biolegend, #422302, 1:20) for 10 minutes ...
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bioRxiv - Genomics 2024Quote: ... for 10 minutes at 4 °C and then stained with FITC - anti-human EPCAM (BioLegend CAT #32404) and PE - anti-CD45 (BioLegend ...
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bioRxiv - Cell Biology 2023Quote: ... Between 50,000 to 100,000 cells were resuspended in 10 uL of PBS supplemented with 2% FBS and 2 mM EDTA (hereafter named “FACS buffer”) together with 5 uL of TruStain FcX (BioLegend, San Diego, CA, USA). Cells were incubated with the FcX block for 10 minutes at RT ...
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bioRxiv - Immunology 2019Quote: ... and anti-CD28 antibody (BioLegend, 2 μg/ml, clone 37.51) before use ...
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bioRxiv - Cancer Biology 2020Quote: ... CD18 (M18/2) and CD62L (MEL-14; all from Biolegend). In some experiments ...
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bioRxiv - Bioengineering 2021Quote: ... AlexaFluor488 anti-Bcl-2 and BV711 anti-CD11b antibodies (Biolegend) were used for staining of in vivo NP-treated alveolar and peritoneal macrophages ...
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bioRxiv - Neuroscience 2020Quote: ... 6-diamidino-2-phenylindole (DAPI) (diluted 1:20’000; BioLegend, #422801). Finally ...
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bioRxiv - Cancer Biology 2022Quote: ... anti-TNF-α BV785 and anti-IL-2 FITC (Biolegend) in PermWash buffer according to the manufacturer’s protocol ...
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bioRxiv - Immunology 2021Quote: ... SARS-CoV-2 ΔRBM RBD-APC (streptavidin-APC from BioLegend). Conjugations were performed as previously described (65) ...
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bioRxiv - Bioengineering 2020Quote: ... ELISA plates were coated with anti-IL-2 Ab (BioLegend) at a concentration of 0.25ug/ml overnight at 4 °C or 1 h at 37 °C ...
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bioRxiv - Immunology 2022Quote: ... CD90-2 (alexa fluor 700 AM; clone 30-H12, BioLegend), B220 (CD45R ...
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bioRxiv - Immunology 2022Quote: ... IL-2 was also neutralized (JES6-1A12, BioLegend, 5ug/mL). Cells were cultured on stimulation for 72-96h before analysis.
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bioRxiv - Cancer Biology 2021Quote: ... suspended in culture medium containing IL-2 (10ng/ml; BioLegend). Cells were directly stained with CFSE (Cayman Chemical ...
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bioRxiv - Immunology 2020Quote: ... anti-IL-2-PE/Cy7 (clone MQ1-17H12, BioLegend, USA).
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bioRxiv - Immunology 2019Quote: ... anti-TNF-α Alexa488 and anti-IL-2 APC (Biolegend) with the Cytofix/CytoPerm staining kit (BD Biosciences ...
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bioRxiv - Developmental Biology 2021Quote: ... Cd49f-BV421 (Biolegend 313623, 2 µg ml−1, 1:100), Cd49b-AF488 (Biolegend ...
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bioRxiv - Immunology 2021Quote: ... and biotin anti-mouse IL-2 (clone JES6-5H4, BioLegend) antibody was used as the secondary antibody ...
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bioRxiv - Immunology 2021Quote: ... LEAF™ purified anti-CD18 (clone M18/2, 101409, BioLegend), LEAF™ Purified Rat IgG2a ...
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bioRxiv - Immunology 2020Quote: ... CD3 AF700 (clone OKT3, BioLegend, cat# 317340, 2:100 dilution); CD4 PerCP-Cy5.5 (clone OKT4 ...
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bioRxiv - Immunology 2020Quote: ... CD45RA BV510 (clone HI100, BioLegend, cat# 304142, dilution 2:100). After cell surface staining ...
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bioRxiv - Immunology 2021Quote: ... anti-IFNγ antibody (2 ug/ml; clone MD-1, Biolegend), anti-IL-4 antibody (2 ug/ml ...
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bioRxiv - Immunology 2021Quote: ... anti-IFNγ antibody (2 ug/ml; clone MD-1, Biolegend), bexarotene (1 μM ...
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bioRxiv - Immunology 2021Quote: ... or biotin anti-mouse IL-2 (Clone: JES6-5H4, BioLegend) SA-HRP (Jackson immunoresearch ...
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bioRxiv - Immunology 2021Quote: ... or purified anti-mouse IL-2 (Clone: ES6-1A12, BioLegend). Plates were then incubated with blocking solution containing 10% FBS in PBST (0.05% Tween-20) ...
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bioRxiv - Neuroscience 2021Quote: ... 6- diamidino-2-phenylindole (DAPI) (diluted 1:20,000; BioLegend, #422801). Finally ...
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bioRxiv - Cell Biology 2022Quote: ... treated with streptavidin-labeled allophycocyanin (APC) (2 μg/mL BioLegend) for 15 min on ice ...
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bioRxiv - Genomics 2022Quote: ... 1 ng/mL of recombinant IL-2 (BioLegend, Cat#575402) and 1 μg /mL of soluble anti-CD28 (Clone ...
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bioRxiv - Immunology 2022Quote: ... anti-TRAV1-2 BV510 (clone 3C10; Biolegend, San Diego, CA), anti-Pan-gδ PE-Vio770 (clone 11f2 ...
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bioRxiv - Immunology 2022Quote: ... with soluble anti-CD28 (2 μg/mL) (ULTRA-LEAF Biolegend) in the presence or absence of a mouse anti-human PD1 (2 μg/mL ...
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bioRxiv - Cancer Biology 2024Quote: ... 50,000 Calcein-labeled (2 µM, Biolegend, San Diego, CA, USA) COLO 205 cells were co-cultured with 500,000 human whole leukocytes for 4 hours with or without 5-A-RU (50 nM ...
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bioRxiv - Immunology 2022Quote: ... and 2 ng/mL recombinant mouse IL-7 (577802; BioLegend) for 28 days ...