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Citations for BioLegend :
201 - 250 of 6674 citations for Primary Human Brain Microvascular Endothelial Cells since 2019
Citations are collected from bioRxiv only, the total number of publications could be much larger.
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bioRxiv - Cell Biology 2022Quote: ... and 0.1% Tween-20 and incubated overnight at 4°C in a humidified chamber with primary human anti-CD9 (Biolegend, catalog no. 312102, dilution 1:1000), human anti-CD63 (BD ...
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bioRxiv - Immunology 2023Quote: ... Human Fc receptors were blocked using Human TruStain FcX (Biolegend).
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bioRxiv - Immunology 2023Quote: ... Human Fc receptors were blocked using Human TruStain FcX (Biolegend). Phenotyping was performed on a NovoCyte Advanteon flow cytometer and FACS was performed on a BD LSRII.
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bioRxiv - Immunology 2024Quote: ... and stained with mouse anti-human HLA-E primary antibody or mouse anti-human HLA class I primary antibody (clone W6/32, purified from hybridoma cells) in 1:100 dilution with cell staining buffer (BioLegend) and a secondary antibody goat anti-mouse antibody conjugated with APC or rat anti-mouse antibody conjugated with Alexa Fluro 700 (BioLegend) ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were then stained with biotin anti-human CD326 (Cat#324216; BioLegend; 1:250) for 30 minutes at 4 °C ...
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bioRxiv - Genomics 2019Quote: ... using antibodies specific to human blood cell surface antigens for CD45 (Biolegend, catalog# 103115), CD3 (Biolegend ...
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bioRxiv - Cell Biology 2020Quote: ... Cells were treated with human or murine cytokines or chemokines (BioLegend, San Diego, CA) for 24 h prior to cell extract preparation ...
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bioRxiv - Bioengineering 2020Quote: ... The cells were then stained by an APC anti-human CD69 antibody (BioLegend, 310910) and analyzed by flow cytometry ...
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bioRxiv - Cell Biology 2021Quote: ... and the cells were washed and incubated with anti-human CD66b FITC (Biolegend #305104) and anti-human Semaphorin 7A-AF647 (R&D #FAB20681R ...
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bioRxiv - Microbiology 2020Quote: ... Cells were resuspended in 1:50 dilution of human FC blocking solution (BioLegend; #422302) and incubated on ice for 10 min ...
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bioRxiv - Immunology 2020Quote: ... this cell line was stained with anti-human CD19-PerCP-Cy5.5 (BioLegend, Clone HIB19). All cell culture was performed at 37°C/5% CO2 in a humidified environment.
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bioRxiv - Cancer Biology 2021Quote: ... Cells were pelleted and stained on ice with PE anti-human IL13Rα2 (Biolegend, 354403). Live cells were labeled with a fixable viability stain (Invitrogen ...
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bioRxiv - Cancer Biology 2019Quote: ... the MojoSort™ Human CD4 naïve T cell isolation kit (catalog; 480041) (BioLegend, CA) was used.
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bioRxiv - Immunology 2019Quote: ... Cells were washed with PBS and blocked with 50 µl/well (Human FcX, BioLegend) for 15 min ...
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bioRxiv - Cell Biology 2021Quote: Freshly isolated cells were blocked with 5μL of Human TruStain FcX™ (Biolegend, USA) and 5μL of True-Stain Monocyte Blocker™ (Biolegend ...
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bioRxiv - Immunology 2020Quote: Human CD8 T cells were stained with the following antibodies: CCR7 (Biolegend clone G043H7), APCCy7 (Biolegend clone SK1) ...
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bioRxiv - Immunology 2021Quote: Single cell suspensions from fluid and tissue were subjected to Human TruStain FcX (Biolegend) in FACS buffer (PBS supplemented with 5% FBS (HyClone) ...
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bioRxiv - Immunology 2022Quote: ... The cells were stained with an anti-human-MR1-APC mAb (clone 26.5, Biolegend) or with APC-labeled mouse IgG2a ...
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bioRxiv - Immunology 2022Quote: ... CD19+ B cells were then stained with FITC anti-human CD19 antibody (BioLegend, 392508), FITC anti-human CD20 antibody (BioLegend ...
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bioRxiv - Cancer Biology 2022Quote: ... Cells were subsequently labelled with APC anti-human PD-L1 (clone 29E.2A3; Biolegend), APC anti-human PD-L2 (clone 24F.10C12PE ...
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bioRxiv - Immunology 2022Quote: ... successfully transduced cells were collected after 10 min incubation with human IFN-α (BioLegend), IFN-γ (BioLegend) ...
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bioRxiv - Immunology 2022Quote: ... Resuspend cells in 100ul FACS staining buffer and add 5ul FcX (human, Biolegend 422302) into each sample at room temperature for 10min ...
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bioRxiv - Neuroscience 2023Quote: ... Cells were stained with 1:100 concentration of CD45 (anti-human PE-Cy7, Biolegend), CD45 (anti-mouse Pacific Blue ...
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bioRxiv - Cancer Biology 2023Quote: ... Cells were then stained with human KI67-APC antibody (Biolegend, Cat#151206, clone 11F6).
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bioRxiv - Immunology 2023Quote: ... The stimulated cells were harvested and stained with anti-human CD80-FITC (305206, BioLegend) for 15 min at room temperature ...
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bioRxiv - Genetics 2023Quote: Bone marrow cells were incubated with Human TruStain FcX (BioLegend, cat# 422302, RRID: AB_2818986) and TruStain FcX (anti-mouse CD16/32 ...
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bioRxiv - Cell Biology 2022Quote: ... Phycoerythrin-conjugated anti-human tetherin (BioLegend, RS38E, FC 2 μg / 100 μl / 106 cells), Pacific Blue-conjugated anti-CD71 (Exbio ...
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bioRxiv - Bioengineering 2023Quote: ... THP-1 cells were supplemented with 100 U/ml recombinant human IFN-γ (BioLegend) for 3 days to induce PD-L1 expression ...
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bioRxiv - Bioengineering 2023Quote: ... T-cells were fluorescently labeled with AlexaFluor 647 anti-human CD3 Antibody (BioLegend #300416) at a concentration of 5 μg/mL ...
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bioRxiv - Bioengineering 2023Quote: ... The cells were then stained with anti-human IgG Fc-APC (Biolegend, Cat#366906) on ice for 30 min and washed with PBS twice ...
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bioRxiv - Immunology 2023Quote: ... Cells were incubated with anti-human CCR2-biotin (FAB151B; R&D Systems/ 357224; BioLegend) in FACS buffer (HBSS ...
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bioRxiv - Genomics 2023Quote: ... For day 19 post CAR T cell delivery analyses anti-human CD45-FITC (Biolegend) staining was added to the above panels to increase sensitivity of CAR T cell detection ...
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Entorhinal cortex vulnerability to human APP expression promotes hyperexcitability and tau pathologybioRxiv - Neuroscience 2023Quote: ... fixed and permeabilized cells were incubated with human-specific APP antibody (SIG-39320, Biolegend) at dilution 1:250 for 1 h ...
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bioRxiv - Cell Biology 2023Quote: ... Cells were then pelleted and stained with anti-human CD45–BV605 (1:100; BioLegend), anti-human CD31–BV421 (1:100 ...
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bioRxiv - Cancer Biology 2024Quote: ... cells were treated with different combinations of 400 ng/mL human recombinant sFasL (Biolegend), 40 μM Fasudil HCl (SellekChem) ...
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bioRxiv - Immunology 2024Quote: ... cells were stained with Pacific Blue™ anti-human CD3 (clone: UCHT1; BioLegend®), Alexa Fluor 647 anti-human CD4 (clone ...
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bioRxiv - Immunology 2021Quote: CD4+ T-cells were isolated from PBMC using the MojoSort™ Human CD4 T Cell Isolation Kit (480009; BioLegend) or by flow-cytometric sorting ...
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bioRxiv - Cell Biology 2022Quote: ... Then cells were resuspended in Maxpar® cell staining buffer (Fluidigm) and blocked with Human TruStain FcX™(Biolegend) for 10 minutes at room temperature ...
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bioRxiv - Immunology 2023Quote: ... Each B cell sample was split into two aliquots of at least 500,000 cells for labeling with different TotalSeqC anti-human cell hashing antibodies (BioLegend). Cells at a concentration of 5 million/ml were first incubated with human Fc block (BD Biosciences ...
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Safety and efficacy of C9ORF72-repeat RNA nuclear export inhibition in amyotrophic lateral sclerosisbioRxiv - Systems Biology 2021Quote: ... cell cultures were incubated with the appropriate primary antibodies (rabbit anti-Nestin, 1:500 [Biolegend #841901] ...
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bioRxiv - Bioengineering 2019Quote: ... Additional primary antibodies used on in vitro-cultured cells include anti-mouse CD31 (Biolegend 102417), Neurofilament (Fisher Scientific MS359R7) ...
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bioRxiv - Synthetic Biology 2023Quote: ... Supernatants from primary cell assays were also collected and analyzed for IFNɣ by ELISA (BioLegend).
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bioRxiv - Cell Biology 2020Quote: ... Brilliant Violet 421 anti-human CD45 and CD31 combined with a panel of cell-surface proteins targeting 332 PE-conjugated monoclonal antibodies (Biolegend, LEGENDScreen Human PE Kit, 700007) and 10 isotype controls (Biolegend ...
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bioRxiv - Immunology 2022Quote: ... using human (BioLegend) and murine (PeproTech ...
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bioRxiv - Immunology 2022Quote: ... anti-human (Biolegend): GZMB-APC (Cat.372203) ...
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bioRxiv - Cell Biology 2021Quote: ... Primary antibodies: ATF4 (BioLegend, 693902 used in primary hepatocytes and liver tissue) ...
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bioRxiv - Neuroscience 2023Quote: ... TMEM119 (mouse primary, Biolegend, 853302 ...
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Antigen-dependent inducible T cell reporter system for PET imaging of breast cancer and glioblastomabioRxiv - Synthetic Biology 2022Quote: ... CD4+ T cells and CD8+ T cells were separated using Biolegend MojoSort Human CD4 T Cell Isolation Kit (Biolegend #480130, San Diego, CA) following manufacturer’s protocol ...
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bioRxiv - Immunology 2024Quote: ... Alexa Fluor® 647 anti-human human CD3 Antibody (BioLegend, 300416), Biotin anti-human CD3 Antibody (BioLegend ...
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bioRxiv - Molecular Biology 2020Quote: ... CD3 T cells were separated from freshly PBMCs by using a MojoSort™ Human CD3+ T cell Isolation Kit (Biolegend, https://www.biolegend.com/protocols/mojosort-isolation-kits-protocol-1/4599/).